Enzyme
Isolated from the plants Perilla frutescens and Gentiana triflora (clustered gentian). Acts on a range of anthocyanidin 3-O-glucosides, 3,5-di-O-glucosides and cyanidin 3-rutinoside. It did not act on delphinidin 3,3',7-tri-O-glucoside. Recombinant Perilla frutescens enzyme could utilize caffeoyl-CoA but not malonyl-CoA as alternative acyl donor.
ANAT
Enzyme
Anthocyanin acyltransferase
Enzyme name
EC:2.3.1.215
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanin-3-O-glucoside 6''-O-acyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3-O-beta-D-glucoside = CoA + an anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [RN:R10237]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [CPD:C20461]
Product
Species information
Aquilegia coerulea
Species name
Rocky mountain columbine (Aquilegia coerulea) is a species of flowering plant in the family Ranunculaceae (buttercup family).
Species info
Gene sequence info
Aquilegia_coerulea.ANAT.fa
Download
>Aqcoe2G375700.1.p
MALTSMVKVLEECRISPPAGSVSTTSLPLTFFDLIFLKIPPVQRLFFFEFNHPKTHFMTTILPNIKHSLSLALQPFYPLAGHITWPQESDIPEILYTDGDSICLTVAESDNDFYHLSGNHPRDANEFYPLVPNFEQIQSSDKLVPILALKITLFPDKGLCIGVTINHIAADGRTSNQFMKTWASISRLHGHGHNTSLPTELTQPLYDRTIINHPKEIEQIRLDILAKVRATKNASTFSAAPLVPVSAGSVRATFVMGRSDVIKLKQWILTRLHNEKQIPAQLHLSTFVVTCAYVWVCLVKARIGNKYVANVSETEDQKEYFAFAMDCRDQLSPPVPVTYFGNCLGFCMATSNRKDLIQEDGIATAAELLGKTIQIARDPATVWELMARRFSNIFSIDLQCVVSIAGSPKLNVYKTDFGWGKPNKVEVISIEGTGAISISEHTKEEGALEVGLVRNKKEMDAFSSLFLNHINSLPKM*
>Aqcoe6G290900.1.p
MELPHTVKVLEECQIAPPSGSVPHTTLPLTFFDLVYLTMPPVHRLFFYNYHYPKAHFIDTVLPNIKKSLSLTLQHFYPFAGNLKWPQESAKPELVYEEDDAVPLTVVESDYEFYQLSGNHVRDATVFPPLIPKLRYTTSNEKLVPLLALQVTLFPGKGICIGITINHVVSDGRTSIHFIKSWASVCRLEGDTSLMTASLPYYDRSIISYLDPKAMDYLNIFEKYNVVTKDTFHTLKVDHLPPIDRVRATFVMGLNDIKRLKKWVSARLEMEAPFNLSSLVVTCAYVWVCLLKARWGETDHENEAEEHIIVPIDARARLDPPLPVTYFGNCIGGIDVMCNRSVLIGEDGIAVAANLIGKAIKNYDFYAQLDRDLSYFMSIPKGQIEGVGSSPRFAVYDTDFGWGKPEKVEMNPIDGGQSIALSECPNEAGAIEIGLVRKKAEMDPFAYSFANSIELLS*
>Aqcoe7G374900.1.p
MEFPQHMVDVVEECKISPPLGSVPCTTLPFTAFDLNLLMLPPVQRLFFYEFHQSKAHFMDTIFPNIKHSLSLTLQHFFPLAGNMIWTQESTIPEFFYKDGDGVSLTLAESTYDFDLLSSNQQRDASMFVPLIPKFSATSSEGVVPLLALQVTFFPNKGICIGVAIKHVVADGRTTTHFIKSWSSICRLEGDTSLISNSLPSHDRSTVKHVKPKRITTNIILMEKLGVTRDTFHQIINKRDAHNTVPVGKVRATFVMLSSDVTKLKQWVLSKEREILHLSSLVVTCAYVWVCLIKARRSASGETDVSANDEATEYFLVPADARARLKPPLPITYFGNCLDSAVATCKRSELIGEDGIAFAAELISKAIKKMDINDETQEKSLSFYISLPREHFSAVSSSPRFAVYETDYGWGKPKKVEFVSGESVSLSECPSVEGGLEIGVVRNKIEMDSFSSLFANTLATLS*
>Aqcoe7G374800.1.p
MEFPQHIVNVVEECQISPPLGSVPTTSLPPTCFDLSFLMLPPVQRLFFYELHHSKAHFMDTILPKIKHSLSITLQHFYPLAGNLTWPQESTIPEFLYKDGDAVTLTLAESDYDFCLLSGNQQRDASAFYPLIPTFKCTTSEKVVPLLALQVTLFPNKGICIGFSIKHVVADGRSYTHFIKSWATICRMEGDISLLIDSLPTYDRSTVKHLKMKKISSNFNVMEKRGVTRDTLHDIINKRDDHHTVPVDKVRATFVLVSSDIEKLKQWILSKEKELLHLSSLVVTCAYVWVCMIKAQKLTLGGPDVNDEVIEYLLLPADARARLKPPLPVTYFGNCLDSAAVTAKRSDVIGEDGIACAAKLISKKIREMDINDDTKEKDLSFYISLPRNHCVAISSSPKFGVYETDYGWGKPKKVEFVSGESMTISECPNVGGGLEIGLVRNKIEMDAFASFFADSLAVFS*
>Aqcoe1G305000.1.p
MGSPDKVQVLEKCRVAPPKGSISSTSLPLTFLDLPWLVIAPVDRLFFYEFEHSKAHFMNTILPNIKHSLSLTLQQYYPLAGNLTWSQESTKPEILYTIGDSVSLTVAECDRDFYNLTGNHARDANEFHPLVPTFAQVNSSEKLVPAMALQVTLFPYKGICIGIKMNHVVADGRTSNHFMKSWATLCRLEGDHTSVATESFVPLYDRSIVKDPKEIEKTLLNDMAKLNITSDTFKFSAVAFVSDKSDNVQATFVIGQSDVKKLKEWISTRLRNEEGILPQLHLSTFVLACAYVWVCLIKAEWVSNDDVVKVSEDQKEQFAFAVDGRSQLNPPVPITYFGNCLGYCLVKSNRKALLQDDGIAIAAELIAKGIQKIRNPDELWTLVANFYCRNPSVPRLDPDCTITVAGSPKLNVYETDFGWGKPKKVEVISIRGSGAISLTEYPHEEGALEIGLVRTKKKMDAFSSLFINNLKSLPSGSGN*
>Aqcoe1G305200.1.p
MGMSCRVRVVEQCRIAPPSDSVSTTTMPLTFFDLMWLALPPVHRIFFYETDHSKAQFTQTIIPNLKHSLSLTLQQFFPLAGNLTWPQESTRPEIQYINGDSVALTVAISDYNFDRLLCNHAKPVKDFYPLVPQLCNTASSKVFPLLALQVTSFPNRGICIGLTLDHVVADGRTFHHFIKSWASTSRLEGKNSTMSESLPCYDRYMVKDPNGMDMVFLNHYKKLNITKESFSITKDPMAPMDKVLASFVLSRTDIKRIKQWVLTRLDNDKERKPPFHLSTFVLTCAYVWVCLIKARWVVSGSEDPIEYFLFPMDCRARLEPPLPNTYFGNCLKSGLIMIKRSDLTGEAAMAIAAELIAKVIEKKVDEDDILKDMETLFSSDFSSAPDQILSVAGSTKLGIYDTDFGWGNPKKSEVISIANTGAILLSECPSEIGAVEIGLVRNKSEMENFASFFTKTVEGFPPIEDDNC*
>Aqcoe1G182900.1.p
MALPYKVEVLEICQISPPLGSVSTTSIPLTFFDLPWLLVPPVQKLLYYELHQPKAYFMNTILPNLKHSLSITLQHFFPLTGRLSWPQDSAKPQILCMDNDSAPLIVAECHYDFHHLSGTLSRNANELYPLAPTFEHAISDGKLIPLLALQVTLFPNKGICLGITFDHAALDGRSLHHFIKSWAAICKSQGGTDFMTDSLPFYDRTIVKDPNGFETINLKDLATFNITKETFNFLKKVPMVPVDKVRNSFVLSSSDIKKLKQQVLSLLDKDKQNVPIFNLSTFVLISAYVWICSIKARWETEKVINVRDCEDQREYFMFPVDCRARLNPPLPLTYSGTCVGGVFVKSDRNALIGENGIAIAAEVIGKGIIDADTEVWTVMEKGFRSYASIPPGQLLTIAGSPNFGIYETDFGWGKPKKSEASLTDSRGAIFFNNHPGEEGAVEILLVRDAIEMEAFASAFTNTLSPALFNSIVGKNKSVAILSGEILPSNL*
>Aqcoe1G182800.1.p
MALPYNVEVLEICQISAPLGSVSTTSLPLTFFDLPWLVLPPVQNIFYYEFHHPKDYFMNTILPNLKHALSLTLQHFCPLTGHFSWPQDSAKPQIVFKDGDSVPLIVAECNYDFNRLLGNHPRNANEFYPLVATYEHAKLTFPLLALQVTLFPNKGICIGITIDHAAMDGRTFHHFIKSWASICKSQGDTSFLTESLPFYDRAIVKDRNGFEIVNLNDLKMINITKETFNSIKKVPTAPFDKVRGTFVMSCSEIKQLKQWVLTHLEKDKKNVPPVHLSTFVLISAYVWICSIKARCGTESAVDVCEDAKEYFMFSVDCRARLDPPLPTAYWGNCIGGVVVKSDRKQLLGENGIAIATKAIGKGIMNADTEVWSLMEKGFSYYTSIPPGQFDTVSGSNKFGIYEIDFGWGNPKKNEANLTDFGGAIYLTDSPNEEGAVEIALVRHAVEMDEFASIFAKTLKVLDS*
>Aqcoe5G119000.1.p
MGLPDTVQVLEQCRIAPPTGSSISTTSLLLTFLDLPMLVLPPIERIFFYEFNHSKSHFMNTILPNIKHSLSLALQHYYPLAGSLTWSQEANTPKILYTIGDTVSLTVAECDHDFYHLAGYHARDDNDFHPLVPMFGRVNSSDKLFPVMALQVTLFPNKGICIGIKLNHAVADGRTSNQFMKLWASFCKLEGNHSLVATDSSLPLYDRSIVKDPKELEKKILKNMANANITRDTFKFLAMPSSVSARADPPVRATFVIGQSDVKKLKQWVLTRLNNKKEIPPQLHLSTFVLGCAYVWVCLIKTRWGADDVALSVDQEERFAFVFDCRSQLNPPVPTTYFGNCLGFSMVICNRKELLQEDGIAIAAELLGKKIQSIRNDPEEIWKPLASFYCGSVDREHMLGVAGSPKLNVYETDFGWGKPKKVEITSTGSSAVSLSEHPIEERALEIGLVLSKKEMDAFASLFENNLKTLPA*
>Aqcoe5G384100.1.p
MELPHTLKVLEECQISPPSGSVPPTSLPLTFFDLVYVKLPPVHRLFFYKYHHPKAHFIDTVLPNIKNSLSLTLQHFYPYAGNLKWPQESPKPELVYEEGDAVPLTVAESDYEFSQLSGNHARDAMTIFPEKGICIGITINHVVSDGRTSIHFIKSWASVCRLEGDTSLMTASLPYYDRSVISYLDPKATNYLNIFEKYNVVTKDTFHTLKVDHLPPIDRVRATFVMGLNDIKRLKKWVSSRLEMEEPIYLSSLVVTCAYVWVCLLKARWGETDHENEAEEHIIVPIDARARLDPPLPVTYFGNCIGAVDVMCNRSVLIGEDGIAIAAKLIGKAIKNYDFNAQLEELDLSYFMSIPKGQIEGVGSSPRFAVYDTDFGWGKPEKVEMNPIDGGQSIALSECPNEAGAIEIGLVRKKTEMDPFAYIFANSIELLS*
MALTSMVKVLEECRISPPAGSVSTTSLPLTFFDLIFLKIPPVQRLFFFEFNHPKTHFMTTILPNIKHSLSLALQPFYPLAGHITWPQESDIPEILYTDGDSICLTVAESDNDFYHLSGNHPRDANEFYPLVPNFEQIQSSDKLVPILALKITLFPDKGLCIGVTINHIAADGRTSNQFMKTWASISRLHGHGHNTSLPTELTQPLYDRTIINHPKEIEQIRLDILAKVRATKNASTFSAAPLVPVSAGSVRATFVMGRSDVIKLKQWILTRLHNEKQIPAQLHLSTFVVTCAYVWVCLVKARIGNKYVANVSETEDQKEYFAFAMDCRDQLSPPVPVTYFGNCLGFCMATSNRKDLIQEDGIATAAELLGKTIQIARDPATVWELMARRFSNIFSIDLQCVVSIAGSPKLNVYKTDFGWGKPNKVEVISIEGTGAISISEHTKEEGALEVGLVRNKKEMDAFSSLFLNHINSLPKM*
>Aqcoe6G290900.1.p
MELPHTVKVLEECQIAPPSGSVPHTTLPLTFFDLVYLTMPPVHRLFFYNYHYPKAHFIDTVLPNIKKSLSLTLQHFYPFAGNLKWPQESAKPELVYEEDDAVPLTVVESDYEFYQLSGNHVRDATVFPPLIPKLRYTTSNEKLVPLLALQVTLFPGKGICIGITINHVVSDGRTSIHFIKSWASVCRLEGDTSLMTASLPYYDRSIISYLDPKAMDYLNIFEKYNVVTKDTFHTLKVDHLPPIDRVRATFVMGLNDIKRLKKWVSARLEMEAPFNLSSLVVTCAYVWVCLLKARWGETDHENEAEEHIIVPIDARARLDPPLPVTYFGNCIGGIDVMCNRSVLIGEDGIAVAANLIGKAIKNYDFYAQLDRDLSYFMSIPKGQIEGVGSSPRFAVYDTDFGWGKPEKVEMNPIDGGQSIALSECPNEAGAIEIGLVRKKAEMDPFAYSFANSIELLS*
>Aqcoe7G374900.1.p
MEFPQHMVDVVEECKISPPLGSVPCTTLPFTAFDLNLLMLPPVQRLFFYEFHQSKAHFMDTIFPNIKHSLSLTLQHFFPLAGNMIWTQESTIPEFFYKDGDGVSLTLAESTYDFDLLSSNQQRDASMFVPLIPKFSATSSEGVVPLLALQVTFFPNKGICIGVAIKHVVADGRTTTHFIKSWSSICRLEGDTSLISNSLPSHDRSTVKHVKPKRITTNIILMEKLGVTRDTFHQIINKRDAHNTVPVGKVRATFVMLSSDVTKLKQWVLSKEREILHLSSLVVTCAYVWVCLIKARRSASGETDVSANDEATEYFLVPADARARLKPPLPITYFGNCLDSAVATCKRSELIGEDGIAFAAELISKAIKKMDINDETQEKSLSFYISLPREHFSAVSSSPRFAVYETDYGWGKPKKVEFVSGESVSLSECPSVEGGLEIGVVRNKIEMDSFSSLFANTLATLS*
>Aqcoe7G374800.1.p
MEFPQHIVNVVEECQISPPLGSVPTTSLPPTCFDLSFLMLPPVQRLFFYELHHSKAHFMDTILPKIKHSLSITLQHFYPLAGNLTWPQESTIPEFLYKDGDAVTLTLAESDYDFCLLSGNQQRDASAFYPLIPTFKCTTSEKVVPLLALQVTLFPNKGICIGFSIKHVVADGRSYTHFIKSWATICRMEGDISLLIDSLPTYDRSTVKHLKMKKISSNFNVMEKRGVTRDTLHDIINKRDDHHTVPVDKVRATFVLVSSDIEKLKQWILSKEKELLHLSSLVVTCAYVWVCMIKAQKLTLGGPDVNDEVIEYLLLPADARARLKPPLPVTYFGNCLDSAAVTAKRSDVIGEDGIACAAKLISKKIREMDINDDTKEKDLSFYISLPRNHCVAISSSPKFGVYETDYGWGKPKKVEFVSGESMTISECPNVGGGLEIGLVRNKIEMDAFASFFADSLAVFS*
>Aqcoe1G305000.1.p
MGSPDKVQVLEKCRVAPPKGSISSTSLPLTFLDLPWLVIAPVDRLFFYEFEHSKAHFMNTILPNIKHSLSLTLQQYYPLAGNLTWSQESTKPEILYTIGDSVSLTVAECDRDFYNLTGNHARDANEFHPLVPTFAQVNSSEKLVPAMALQVTLFPYKGICIGIKMNHVVADGRTSNHFMKSWATLCRLEGDHTSVATESFVPLYDRSIVKDPKEIEKTLLNDMAKLNITSDTFKFSAVAFVSDKSDNVQATFVIGQSDVKKLKEWISTRLRNEEGILPQLHLSTFVLACAYVWVCLIKAEWVSNDDVVKVSEDQKEQFAFAVDGRSQLNPPVPITYFGNCLGYCLVKSNRKALLQDDGIAIAAELIAKGIQKIRNPDELWTLVANFYCRNPSVPRLDPDCTITVAGSPKLNVYETDFGWGKPKKVEVISIRGSGAISLTEYPHEEGALEIGLVRTKKKMDAFSSLFINNLKSLPSGSGN*
>Aqcoe1G305200.1.p
MGMSCRVRVVEQCRIAPPSDSVSTTTMPLTFFDLMWLALPPVHRIFFYETDHSKAQFTQTIIPNLKHSLSLTLQQFFPLAGNLTWPQESTRPEIQYINGDSVALTVAISDYNFDRLLCNHAKPVKDFYPLVPQLCNTASSKVFPLLALQVTSFPNRGICIGLTLDHVVADGRTFHHFIKSWASTSRLEGKNSTMSESLPCYDRYMVKDPNGMDMVFLNHYKKLNITKESFSITKDPMAPMDKVLASFVLSRTDIKRIKQWVLTRLDNDKERKPPFHLSTFVLTCAYVWVCLIKARWVVSGSEDPIEYFLFPMDCRARLEPPLPNTYFGNCLKSGLIMIKRSDLTGEAAMAIAAELIAKVIEKKVDEDDILKDMETLFSSDFSSAPDQILSVAGSTKLGIYDTDFGWGNPKKSEVISIANTGAILLSECPSEIGAVEIGLVRNKSEMENFASFFTKTVEGFPPIEDDNC*
>Aqcoe1G182900.1.p
MALPYKVEVLEICQISPPLGSVSTTSIPLTFFDLPWLLVPPVQKLLYYELHQPKAYFMNTILPNLKHSLSITLQHFFPLTGRLSWPQDSAKPQILCMDNDSAPLIVAECHYDFHHLSGTLSRNANELYPLAPTFEHAISDGKLIPLLALQVTLFPNKGICLGITFDHAALDGRSLHHFIKSWAAICKSQGGTDFMTDSLPFYDRTIVKDPNGFETINLKDLATFNITKETFNFLKKVPMVPVDKVRNSFVLSSSDIKKLKQQVLSLLDKDKQNVPIFNLSTFVLISAYVWICSIKARWETEKVINVRDCEDQREYFMFPVDCRARLNPPLPLTYSGTCVGGVFVKSDRNALIGENGIAIAAEVIGKGIIDADTEVWTVMEKGFRSYASIPPGQLLTIAGSPNFGIYETDFGWGKPKKSEASLTDSRGAIFFNNHPGEEGAVEILLVRDAIEMEAFASAFTNTLSPALFNSIVGKNKSVAILSGEILPSNL*
>Aqcoe1G182800.1.p
MALPYNVEVLEICQISAPLGSVSTTSLPLTFFDLPWLVLPPVQNIFYYEFHHPKDYFMNTILPNLKHALSLTLQHFCPLTGHFSWPQDSAKPQIVFKDGDSVPLIVAECNYDFNRLLGNHPRNANEFYPLVATYEHAKLTFPLLALQVTLFPNKGICIGITIDHAAMDGRTFHHFIKSWASICKSQGDTSFLTESLPFYDRAIVKDRNGFEIVNLNDLKMINITKETFNSIKKVPTAPFDKVRGTFVMSCSEIKQLKQWVLTHLEKDKKNVPPVHLSTFVLISAYVWICSIKARCGTESAVDVCEDAKEYFMFSVDCRARLDPPLPTAYWGNCIGGVVVKSDRKQLLGENGIAIATKAIGKGIMNADTEVWSLMEKGFSYYTSIPPGQFDTVSGSNKFGIYEIDFGWGNPKKNEANLTDFGGAIYLTDSPNEEGAVEIALVRHAVEMDEFASIFAKTLKVLDS*
>Aqcoe5G119000.1.p
MGLPDTVQVLEQCRIAPPTGSSISTTSLLLTFLDLPMLVLPPIERIFFYEFNHSKSHFMNTILPNIKHSLSLALQHYYPLAGSLTWSQEANTPKILYTIGDTVSLTVAECDHDFYHLAGYHARDDNDFHPLVPMFGRVNSSDKLFPVMALQVTLFPNKGICIGIKLNHAVADGRTSNQFMKLWASFCKLEGNHSLVATDSSLPLYDRSIVKDPKELEKKILKNMANANITRDTFKFLAMPSSVSARADPPVRATFVIGQSDVKKLKQWVLTRLNNKKEIPPQLHLSTFVLGCAYVWVCLIKTRWGADDVALSVDQEERFAFVFDCRSQLNPPVPTTYFGNCLGFSMVICNRKELLQEDGIAIAAELLGKKIQSIRNDPEEIWKPLASFYCGSVDREHMLGVAGSPKLNVYETDFGWGKPKKVEITSTGSSAVSLSEHPIEERALEIGLVLSKKEMDAFASLFENNLKTLPA*
>Aqcoe5G384100.1.p
MELPHTLKVLEECQISPPSGSVPPTSLPLTFFDLVYVKLPPVHRLFFYKYHHPKAHFIDTVLPNIKNSLSLTLQHFYPYAGNLKWPQESPKPELVYEEGDAVPLTVAESDYEFSQLSGNHARDAMTIFPEKGICIGITINHVVSDGRTSIHFIKSWASVCRLEGDTSLMTASLPYYDRSVISYLDPKATNYLNIFEKYNVVTKDTFHTLKVDHLPPIDRVRATFVMGLNDIKRLKKWVSSRLEMEEPIYLSSLVVTCAYVWVCLLKARWGETDHENEAEEHIIVPIDARARLDPPLPVTYFGNCIGAVDVMCNRSVLIGEDGIAIAAKLIGKAIKNYDFNAQLEELDLSYFMSIPKGQIEGVGSSPRFAVYDTDFGWGKPEKVEMNPIDGGQSIALSECPNEAGAIEIGLVRKKTEMDPFAYIFANSIELLS*
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University