Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Secale cereale
Species name
Rye (Secale cereale) is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Secale_cereale.HI4_OMT.fa
Download
>ScWN1R01G452600.1
MALTVKSTDDRALLDAEHELWATTFSYIKSMALKSALDLRLADAIHHHGGAATLPQIVARVAVHPSKIPCLRRLMRTLTVSGVFSVVEQQDVVDPAASVSNGTSNGAAAAEPSYALTPVSRLLVGSRNLVSMITMLLSPNFVTSFLGIGAWFEHALPDPCLFTQAHGKALWTMADRDATFDALINDAMVSDSSFIMDIAIRECGEVFQGIGSLMDVGGGHGAAAQAISRAFPRLECTVMDLDHVVAKAPAGTDVKYVVGDMFQSVPPADAVFLKWVLHDWGHEDCVKILKNCKKSISPIGKGGKVIIIDIVIGAGPSHLKHRELHSMFDLYMTIVDGIERDEQEWKKIFLEAGFSGYTIIPVLGFRSIIEVYP
>ScWN1R01G452800.1
MAPTREQHILDQGLLDAQLELWHHTFSYVKSMALKSALDLGIADAIHRQGGAATLSQIAATATLHPTKISCLRRLMRVLIVSGIFSVDHPSDDGVEGEAVYALTPASRLLVCSASVNMVHITKMLLHTNLVSPLSDLGTWFQHELPEPDLFKLKHGKTFWELTGQDPAYNALVNDGMVSDSSFIMDIAIRECGAVFQGIGSLVDVAGGYGGAAQAISKAFPDVKCSVMDLAHVVAEAPNGTDVEYIAGDMFESVPPADAVFLKWVMHDWGDEDCIKILKNCKKAIAPRDVGGKVIIVDIVVGAGPQDPKHKETQVMFDLFIMFINGIERDEHEWKKIIFEAGFNDYKITPILGVRSIIERSARFSANNTTLRYPHYGKSKLLACSQST
>ScWN2R01G657800.1
MAMASSAELMQAEADLMRHSLGYLKSMALHSAVKLGVADALHRCGGSASLPDLLATLPLPPSKQPYLSRLMKVLATEGIFVAEEGVNCGDEATCVYHLNTMSRLLVNDAGINGGSAWKLSSCVLIATTPQFVGSALQLGQWFQSDGEATPFVMANGRPPYGVAAHDAEFNSVFNEAMAADSRYLADLVVRECGEVFEGITSLVDVAGGTGTMARAITKAFPHVKCSVLDLPHVIQGVSAPADNVEFVAGDMMEFIPPADRILLKYVLHNWNDQDCVKILTRCREAIAHGEKEGKVIIIDEVVGSGSHNILQAQLLMDMQMISLFMAKERYEQDWNKIFTEAGFVNWKIRPILGVRSVIELYI
>ScWN2R01G021500.1
MEEQDEGHSDAELLQATNELMDHTLSFVRSMALGCAVKLGVADAIHRGGGHVFLHDLIAALPMAPNKLPHLCRLMRVLTTSGVFAQADDGAYCLTPVSSLLVSDGGKRKNQTPLVRLHLSPLAVSPFTNMAEWFTSDDGEETAFKMTHGTDFWGFCDRHVEFNKLFNEAMAADSQFIMEAAIHGAGCQVFEGIMSLVDVGGGTGEAAKAVARAFPHIKCIVLDRPQVIDGIPAGGLVEFVGGDFMDFIPQADALLLKFVLHDWSDKDCVRILKRCKEAIPPRQAGGKVIIIDIVVGSCSKATYQATQQLYDLHMSMVTPGMERDENEWCKLFMESGFTKYKINRVLGLRSIIEVFP
>ScWN4R01G293100.1
MATSSAELLQAEAELVRHSLGYLKSMALHSAVKLGIPDALHRSGGAASLPDLSSALALPCSKRPYLSRLMKMLAVEGIFTAVAAVDVPAAGGGEGAAAAPSVRYGLNPVSRLLVSGSSGACLSPCVLMGTSPLFLEASLRLPQWFQRDDGEGEPAFAMAHGESPYAAAGHDVEFNALVNEAMGSDSRYMAELVVRECGEVFAGVTSLVDVGGGNGTMATAIAKAFPHVRCSVLDLPHVVQGVSAHESVEFAAGDMMEYVPPADAVLLKCVLHNWSDEDCVKILTKCREAIAQGAKAGAGKVIIIDAVVGSPSHSHQVLEAQVLMDMQMMMLFMSKEREELDWQKIFMEAGFSHYKIQPVLGMRSIIQLYP
>ScWN5R01G678400.1
MALNGQKSSALADAELELWTNTFSYIKSMALKSALDLRLADAINHHGGAATLSQIVARVTLHPSKIPCLRRLMRVLTLTGVFSVEQDQAASTDDEPTYGLTPASRLLVGSHNLASMMAMLLNPIMLTPFLGIGDWFKHAQPDPSLFEQTHGDGLWKMAHRDATFGALINDGMVSDSRFIMDIAVKECGEVFQGITSLVDVGGGLGAASQAISRAFPHLECTVMDLGHVIANAPTGTDVKFVAGDMFDSVPRADAVFLKWVLHDWSDEECVRILKNCKEAISAQEGGKVIIMDIVIGAGLSDAKHREMQAMFDLYMIIINGIERDEQEWMKIFLEAGFSRYKIMPVQSFRSIIEVYP
>ScWN6R01G390200.1
MAHVQAENVVMSADDRLQAQLELYHHSLAFVKSMALRAAADLRIPEAIHRHGGTATLSDIASDTGIHATKLTHLRRLMRVLTITGIFSSSGDAVFKLTPVSRLLAEGSESSRNLSPVVVGVFVNPVAVTALLSICEWFTDERAAALSLFEVAHGCTRWEMAAKEKGDSSLLDAGMIADSTIIMEVLLRDHGSIFEGVSSLVDVGGGHGAVAAAIARAFPAVNCTVLDLPHVVAGAPADENIQFVAGDMFEHIPSADVVLLKWIMHCWQDEHCINILRRCKEAIPSREAGGKVIIIDMVVGYAGAASEENVSKETQVLSDVYKMYMDGVEREEDEWAKIFYEAGFSDYKISPVLGFRSIIEVYP
>ScWN7R01G531100.1
MTLKLLAEVSPQELLQALAELQNHMLGYVKSMSLRCVVDLGIPEAIHCRGGTATLADIATDAKVHPCKVPDLQRLCIRHALTTSGIFTATANTGGGDTVVYGLTTACRILVGWRNLSPMVPFILNPLVVSSFFSMPEWFRSEPEAAGAGSLFELAHGCSQWEMVSKDTGFNNIVNDSMAADSRVFLEVIIVDKGRIFRGLRLLVDVGGGNGAGTQVIAKAFPRSKCTVMDLPHVVGQAATGDDNLSFVAGDMFECIPSADAILLKNILHDWGHDDCVKILQRCKEAIPARNAGGKVIIIDMVRGSANGDRKINEMEAIQNLFMMYITGVERDEIEWKRIFSDAGFSDDYKILPVLGPYSVIEIYP
>ScWN7R01G572500.1
MAHVQTENEVMSTEVFLQAQIELYNLCFSYVKSVALRAAIDLQIPDAIHRRGGSATLSDVAAETRVHGSKVSHLRRLMRVLTISGVFSAEGRDGDVVYKLTQVSGLLVAGALSPIVPVLVDPLGATALFSLGEWFTDERASALTLFEVAHGCTRSEMTAKKGTGGLFNAGMVADSRIVMEILLNEHMGIFEGVGSLVDVGGGHGAAAAAVAKALPHVKCTVLDLPHVVQRAPTGGNVQFVAGDVFQYVPPADAVLLKWVMGQWQDEDAVKVLRRCKEAIPAGGKVIIFEAVVGSDSGEDAPHRETQLLFDIFMMRVDGGGERDEQQWKKIIFEAGFSDYKISAVLGFRSIIEAYP
>ScWN7R01G530800.1
MTLKLLAEVSPQELLQALAELQNHMLGYVKSMSLRCVVDLGIPEAIHCRGGTATLADIATDAKVHPCKVPDLQRVMELLTTSGIFTATANTGGGDTVVYGLTTACRILVGWRSLSPMVPFLINPLVVSSFFSMPEWFRSEPDAAGAGSLFELAHGCSQWEMVSKDAAFNNVLNDSMAADSRVFLEVIIVDKGRIFRGLRSLVDVGGGNGAGTQVIAKAFPRIKCTVMDLPHAVGQAAAGDDNLSFVPGDMFESIPSADAILLKNILHDWGHDDCVKILQRCKEAIPARNAGGKVIIIDMVRGSANVDRKINEMEAIQNLFMMYITGVERDEIEWKKIFSDAGFSDDYKILPVLGPYSVIEIYP
>ScWNUn01G042200.1
MALVREQHILDQGLLDAQLELWHHTFSYVKSMALKSALELGIADAIHRQGGVATLSQIAATATLHPSKISCLRRLMRALTVSGIFGVEHSGDGGVEGEAVYALTPASRLLVGSASANMVHFTKMLLHPILVSPFSELGTWFQHELPEPNLFKLKHGKTFWELANQDPAYNALVNDGMVSDSSFLMDIAIRECGGIFQGICSLVDVAGGHGGAAQAISKAFPDVKCSVMDLAHVVAEAPNGTNVEYIAGDMFESVPPADAVFLKWVMHDWGDEDCIKILKNCKKAIAPKDVGGKVIIVDMVVGAGSQGPKHKETQVMFDLFIMFVNGIERDEQEWKKIIFEAGFSDYKITPVLGVRSIIEVYP
MALTVKSTDDRALLDAEHELWATTFSYIKSMALKSALDLRLADAIHHHGGAATLPQIVARVAVHPSKIPCLRRLMRTLTVSGVFSVVEQQDVVDPAASVSNGTSNGAAAAEPSYALTPVSRLLVGSRNLVSMITMLLSPNFVTSFLGIGAWFEHALPDPCLFTQAHGKALWTMADRDATFDALINDAMVSDSSFIMDIAIRECGEVFQGIGSLMDVGGGHGAAAQAISRAFPRLECTVMDLDHVVAKAPAGTDVKYVVGDMFQSVPPADAVFLKWVLHDWGHEDCVKILKNCKKSISPIGKGGKVIIIDIVIGAGPSHLKHRELHSMFDLYMTIVDGIERDEQEWKKIFLEAGFSGYTIIPVLGFRSIIEVYP
>ScWN1R01G452800.1
MAPTREQHILDQGLLDAQLELWHHTFSYVKSMALKSALDLGIADAIHRQGGAATLSQIAATATLHPTKISCLRRLMRVLIVSGIFSVDHPSDDGVEGEAVYALTPASRLLVCSASVNMVHITKMLLHTNLVSPLSDLGTWFQHELPEPDLFKLKHGKTFWELTGQDPAYNALVNDGMVSDSSFIMDIAIRECGAVFQGIGSLVDVAGGYGGAAQAISKAFPDVKCSVMDLAHVVAEAPNGTDVEYIAGDMFESVPPADAVFLKWVMHDWGDEDCIKILKNCKKAIAPRDVGGKVIIVDIVVGAGPQDPKHKETQVMFDLFIMFINGIERDEHEWKKIIFEAGFNDYKITPILGVRSIIERSARFSANNTTLRYPHYGKSKLLACSQST
>ScWN2R01G657800.1
MAMASSAELMQAEADLMRHSLGYLKSMALHSAVKLGVADALHRCGGSASLPDLLATLPLPPSKQPYLSRLMKVLATEGIFVAEEGVNCGDEATCVYHLNTMSRLLVNDAGINGGSAWKLSSCVLIATTPQFVGSALQLGQWFQSDGEATPFVMANGRPPYGVAAHDAEFNSVFNEAMAADSRYLADLVVRECGEVFEGITSLVDVAGGTGTMARAITKAFPHVKCSVLDLPHVIQGVSAPADNVEFVAGDMMEFIPPADRILLKYVLHNWNDQDCVKILTRCREAIAHGEKEGKVIIIDEVVGSGSHNILQAQLLMDMQMISLFMAKERYEQDWNKIFTEAGFVNWKIRPILGVRSVIELYI
>ScWN2R01G021500.1
MEEQDEGHSDAELLQATNELMDHTLSFVRSMALGCAVKLGVADAIHRGGGHVFLHDLIAALPMAPNKLPHLCRLMRVLTTSGVFAQADDGAYCLTPVSSLLVSDGGKRKNQTPLVRLHLSPLAVSPFTNMAEWFTSDDGEETAFKMTHGTDFWGFCDRHVEFNKLFNEAMAADSQFIMEAAIHGAGCQVFEGIMSLVDVGGGTGEAAKAVARAFPHIKCIVLDRPQVIDGIPAGGLVEFVGGDFMDFIPQADALLLKFVLHDWSDKDCVRILKRCKEAIPPRQAGGKVIIIDIVVGSCSKATYQATQQLYDLHMSMVTPGMERDENEWCKLFMESGFTKYKINRVLGLRSIIEVFP
>ScWN4R01G293100.1
MATSSAELLQAEAELVRHSLGYLKSMALHSAVKLGIPDALHRSGGAASLPDLSSALALPCSKRPYLSRLMKMLAVEGIFTAVAAVDVPAAGGGEGAAAAPSVRYGLNPVSRLLVSGSSGACLSPCVLMGTSPLFLEASLRLPQWFQRDDGEGEPAFAMAHGESPYAAAGHDVEFNALVNEAMGSDSRYMAELVVRECGEVFAGVTSLVDVGGGNGTMATAIAKAFPHVRCSVLDLPHVVQGVSAHESVEFAAGDMMEYVPPADAVLLKCVLHNWSDEDCVKILTKCREAIAQGAKAGAGKVIIIDAVVGSPSHSHQVLEAQVLMDMQMMMLFMSKEREELDWQKIFMEAGFSHYKIQPVLGMRSIIQLYP
>ScWN5R01G678400.1
MALNGQKSSALADAELELWTNTFSYIKSMALKSALDLRLADAINHHGGAATLSQIVARVTLHPSKIPCLRRLMRVLTLTGVFSVEQDQAASTDDEPTYGLTPASRLLVGSHNLASMMAMLLNPIMLTPFLGIGDWFKHAQPDPSLFEQTHGDGLWKMAHRDATFGALINDGMVSDSRFIMDIAVKECGEVFQGITSLVDVGGGLGAASQAISRAFPHLECTVMDLGHVIANAPTGTDVKFVAGDMFDSVPRADAVFLKWVLHDWSDEECVRILKNCKEAISAQEGGKVIIMDIVIGAGLSDAKHREMQAMFDLYMIIINGIERDEQEWMKIFLEAGFSRYKIMPVQSFRSIIEVYP
>ScWN6R01G390200.1
MAHVQAENVVMSADDRLQAQLELYHHSLAFVKSMALRAAADLRIPEAIHRHGGTATLSDIASDTGIHATKLTHLRRLMRVLTITGIFSSSGDAVFKLTPVSRLLAEGSESSRNLSPVVVGVFVNPVAVTALLSICEWFTDERAAALSLFEVAHGCTRWEMAAKEKGDSSLLDAGMIADSTIIMEVLLRDHGSIFEGVSSLVDVGGGHGAVAAAIARAFPAVNCTVLDLPHVVAGAPADENIQFVAGDMFEHIPSADVVLLKWIMHCWQDEHCINILRRCKEAIPSREAGGKVIIIDMVVGYAGAASEENVSKETQVLSDVYKMYMDGVEREEDEWAKIFYEAGFSDYKISPVLGFRSIIEVYP
>ScWN7R01G531100.1
MTLKLLAEVSPQELLQALAELQNHMLGYVKSMSLRCVVDLGIPEAIHCRGGTATLADIATDAKVHPCKVPDLQRLCIRHALTTSGIFTATANTGGGDTVVYGLTTACRILVGWRNLSPMVPFILNPLVVSSFFSMPEWFRSEPEAAGAGSLFELAHGCSQWEMVSKDTGFNNIVNDSMAADSRVFLEVIIVDKGRIFRGLRLLVDVGGGNGAGTQVIAKAFPRSKCTVMDLPHVVGQAATGDDNLSFVAGDMFECIPSADAILLKNILHDWGHDDCVKILQRCKEAIPARNAGGKVIIIDMVRGSANGDRKINEMEAIQNLFMMYITGVERDEIEWKRIFSDAGFSDDYKILPVLGPYSVIEIYP
>ScWN7R01G572500.1
MAHVQTENEVMSTEVFLQAQIELYNLCFSYVKSVALRAAIDLQIPDAIHRRGGSATLSDVAAETRVHGSKVSHLRRLMRVLTISGVFSAEGRDGDVVYKLTQVSGLLVAGALSPIVPVLVDPLGATALFSLGEWFTDERASALTLFEVAHGCTRSEMTAKKGTGGLFNAGMVADSRIVMEILLNEHMGIFEGVGSLVDVGGGHGAAAAAVAKALPHVKCTVLDLPHVVQRAPTGGNVQFVAGDVFQYVPPADAVLLKWVMGQWQDEDAVKVLRRCKEAIPAGGKVIIFEAVVGSDSGEDAPHRETQLLFDIFMMRVDGGGERDEQQWKKIIFEAGFSDYKISAVLGFRSIIEAYP
>ScWN7R01G530800.1
MTLKLLAEVSPQELLQALAELQNHMLGYVKSMSLRCVVDLGIPEAIHCRGGTATLADIATDAKVHPCKVPDLQRVMELLTTSGIFTATANTGGGDTVVYGLTTACRILVGWRSLSPMVPFLINPLVVSSFFSMPEWFRSEPDAAGAGSLFELAHGCSQWEMVSKDAAFNNVLNDSMAADSRVFLEVIIVDKGRIFRGLRSLVDVGGGNGAGTQVIAKAFPRIKCTVMDLPHAVGQAAAGDDNLSFVPGDMFESIPSADAILLKNILHDWGHDDCVKILQRCKEAIPARNAGGKVIIIDMVRGSANVDRKINEMEAIQNLFMMYITGVERDEIEWKKIFSDAGFSDDYKILPVLGPYSVIEIYP
>ScWNUn01G042200.1
MALVREQHILDQGLLDAQLELWHHTFSYVKSMALKSALELGIADAIHRQGGVATLSQIAATATLHPSKISCLRRLMRALTVSGIFGVEHSGDGGVEGEAVYALTPASRLLVGSASANMVHFTKMLLHPILVSPFSELGTWFQHELPEPNLFKLKHGKTFWELANQDPAYNALVNDGMVSDSSFLMDIAIRECGGIFQGICSLVDVAGGHGGAAQAISKAFPDVKCSVMDLAHVVAEAPNGTNVEYIAGDMFESVPPADAVFLKWVMHDWGDEDCIKILKNCKKAIAPKDVGGKVIIVDMVVGAGSQGPKHKETQVMFDLFIMFVNGIERDEQEWKKIIFEAGFSDYKITPVLGVRSIIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University