Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Sclerocarya birrea
Species name
Sclerocarya birrea is a species of eudicot in the family Anacardiaceae (sumac family).
Species info
Gene sequence info
Sclerocarya_birrea.ANR.fa
Download
>Sclbi88S16948_SCLBI
MEKRNSKVCVTGGTGYFGSWLVKKLLEKGYTVHSTLRNLDKELLGYGSNVDGDSLEVITLAVGLVGGDSLLSYTPSTVAMYISQLTNSKYLYQALRFTEDLLGKIPMVHIDDACEAHIFCMEKPSINGRFLCASSYVSSAEIANYYQENYPEFHVKQEYLDGPKREIKWGGTKLMEKGFEYKYDTKMILDASIKCARRTGGLNQCKEYCFFALLLKFSWDSRSNKLIPMVLLKVPAVNMGSNR
>Sclbi147S07671_SCLBI
MAYLLSPPNPQRSVSETESSMCACACIYIYIYTGRRLRLHSKQAMDQIKGNVCVTGASGFLASWLVKRLLLAGYHVTGTVRDPGNEKKLAHLWRLEGAKERLRLVRANLMEEGSFDDAIKGCQGVFHTASPVVKPSFDPKACCVPTPSPVMLIDQDYLHIRALRYSYLASLACDIHHDQKPVNTNLLGIFPPTQAEILEPAVKGTLNVLRSCKKNPSLRRVVLTSSSSTVRARDDFDPKIPLDESSWSSVDLCEKFQIWYALSKTQAERAAWAFCEENRIDLVTVLPSFVVGPSLPPDLCSTASDVLGLLKGEAEKFQWHGRMGYVHIDDVALCHILVYEHKHCSGRYLCSSTVVDNNELASLLSSRYPWLRLQLRFEQLNRPYYEFNTSKLTSLGFKFKSIEVMFDDCIAWFVEQGYIRDKEQSYRNN
>Sclbi307S11192_SCLBI
MKSVLTYILYIIHRTHCKKCLALAAAELEEMEDLVSTVEGKREGDKSTYCVTGATGYIGSWLVKSLLERGCIVHATIRDPESYVQSNIIDPAIKGTLNLLRACLKSGSVKRVVFTSSISTITAKDNKGKWRPVVDESCQTPIDHVWNTKASGWVYVLSKLLTEEAAFKFAKENNIDLVSVITTTVAGPFLTSSVPSSIHVLLSPITGDSKFFSILTAVYTRMGSVALVHIEDVCNAHIFLMENAGAEGRFICCARSCSMSELVDHLNQEYPSSNKHSSIPSEISSKKLRDLGFTYNHGVEDIIRQTMASCLDCGLLSPIIK
>Sclbi1865S04675_SCLBI
MILDQPAELIDPALKGTLNVLGSCAKVQSIRRVVVTSSMASVYYNGNPLTTDVVVDETWFSNPALCKE
>Sclbi1865S09121_SCLBI
MLNFSVEVILNIINGAQTFPRPYRLVDVRDVAYVHIQALEVPTASGRYLLVERVAQLCEILKILREHYPTPHLPEKFKAAKYEPTDQISKERAKSLGTDFMPWGVSIRDTVESLKEKGFLNVYMHFGIRMHPFGIHLGSSISISSEINLILIVFQYWYHLSKTLAEEASWILVDDDGIDWVTLHPGSVIGPFLQSVLNFSVEGIENEWRGEGGVCNRSFRLHSFVAELIDPAVKGTLNVLRSSAKVESVKRVVVTSSIATVLFNGHSLTPDVVVDETWFSDPAFCTESKYWYLLSKTLAEEAACKFAKENGIELVTLHPGLVIGPFLQSTLNLSLELILDIINGAETWPSPFTVVDVRDVACAHIQALEVPTASGRYLLVERVSQLYEVLKILHENYPIFHLPEKFGDVKNEPTYQLSNERAKSLGVNFMPLEESLKDTVECFKEKGLLNLLLQRGYTVKATAELIDPAVKGTLNVLRSCAKFQSVKRVVVTSSLATVSFSGSPMTSDVVIDETWFSNSSFCKESKLWYHLAKTLAEEAAWKFAKENGIDLVPLHPGVVIGPFLQPKLNLSVAWILRLINGDQTLSSPFKLVDVRDVAFAHIQALEIPTASGRYLLVGSITHRSEIFEMLHQHYPTLQLPESGNNTNETTYQICKERAKSLGINFIPWEVSFRDTFENFKEKGFLNFTQIRRDRENEWRKEGGGCNRSFSCYSNVVTLSKPLFVTQAELIDPAVKGTLNVLRSCAKAQSVKKVVVTSSFASVFYNGNPLTPDVVVDETWFSNPTLCKESQNWYLLSKTLAEEAAWKFAKESGIDLVTLHPGFVIGPFLQSVPNFTVEVILNLTNGTQTYPSPYRLVDVRDVALAHIQALEVPTASGRYLLVGRVTQLCEILKTLHEHYPTLHLSEKFEASKCELTYQISQERAKSLGINFIPWEVSLRDTVESLKEKDDPKVQHLHALDGAKERLHFIFSPVFQAELIDPAVKGTLNVLRSCAKVQSVKRVVVTSSSTSVIFNGSPLTPDVVVDETWFSDPAFCKESKLWYALSKTLAEEAAWKFAKENAIDLVTLHPGFVIGPFLHSKLNASMEVILNMIKGVETGRGPHRFVDVRDVAYAHIQALEVPTSSGRYLLVGRVTQNCEVQKTLHEHYPTLHCPEKFEAANYEPTYQFSKEKAQSLGVSFMPWEVSLRDTVESLKEKGFLSVEKATEK
>Sclbi1865S04674_SCLBI
MWWVPDIEEYDPSYDVRWASEVTKCSSNEEVDNINLKSQRTIEDRLQMLEGASGYIASCLVKQLLEHDYTVKATVRDPNDSSKIKHLHALDGAKERLRIVKANLLEEGSFDSAVDGCEGVFHTASPVLLSSNEPQSVKRVIITSSMASVCFNERPLTPDVVIDETWFSDPTFCKESKIWYALSKTLAEEAAWKFAKENAIDLVTLHPGFVIGPFLQSMLNVSMEAILNIIKVAETFPSPYRFVDVRDVACAHIQALEIPAANGRYLLVGRVTKRCEVLKILYEHYPTLHLPEKFGAANYELTYQISKERAKSLGVNFMPLEVSLRNTVESFVEKDDSSRIKHLHELDGAKERLHLFNANLLKEGSFDSAVDGCEGVFPTASPVLLSSNEPQVNLILIYFQYWYHLSKTLAEEAAWSFAKENGTDLVTLHPGLSMPTFSVAVILNLVNGNNQQAFEDNECGRKTFPSPYRLVDVRDVAYARIQALEVPTASGRYWLVGRVTQLYEILKILHEHYPNFHLPEKFGDVKHEPVFQVCNERARSLGVNLMPWDVSLRDTLESLSRKASSVADKLLKGSGRSIVSIPDQSNKHGLFYDKLSCTSPFNHVKNICN
>Sclbi2838S10743_SCLBI
MALEKGRVCVTGAGGFLASWVVKLLLSKDYFVHGTVREPSDEKNAHLYKLDKASENLKLFKADLLDYDSLFSAISGCSGVFHVASPVPSIVVEVIEPAVKGTLNVLKACHEAKVKRVVYVSSVAAVGLNPSWPKDRLVDETSWSDKEYCRTTKNWYCLSKTEAEREALEYGKRTGLDVVSICPTLISGPLLQSTLNSSSLVLVKLVKEGYESVENKLRMIVDVRDVAEALLLAYEKPEAEGRYICTAHLIRAQDLVDKLKSIYPNYIYPKNFTDAGEERRPTTEKLQKLGWKMALEKGRVCVTGAGGFLASWVVKLLLSRHYFVHGTVQVIEPAVKGTLNVLKACVEAKIKRVVLVSSDAAIFMNPGWPKGQVMDETCWSDKEYCRTTKNWYYLSKTEAESEALEYAKRSGLDLVTICPTLILGPLLQSLVNASSLILIKLLKVLHIEGYESVENKLWRIVDVRDVAEALLLAYEKPEAEGRYICMAHMIETKDLVDKLKNMYPNYKYPKSFTEEGERIMLSSEKLQRLDLTKGNRKSNESLDWKGVTEMDVRVREEKERVCVTGAGGFIASWLVKFLLSKGYMVHGTNYYCLAKTMAESEALEYAEKGELDIVTVCPSIVLGPMLQTTINASSLLLLALLKGLFFHSVFQYTSFEGVEMTWKLDEHKSIEDKDRPLVDVRDVAEALLLVYEKPEAEGRYICTSYRIRMQALAEKLKSMYPNYSYPKSFTSDEDEVKLSSERLQKLGWKYRPLEETINKERVCVTGAGGYLASWVVKFLLSQAYLGHGTVRDLSDNKNAHLKKLEMASENLQLFKIDLRDNEGLCAAIDGCESVFHVACPVPPVGSVENPEVELLQPSVSGTRNVLNASLKAEVKEVWLCHLLGLLVKPELAQGSAHQWYCLAKTIAESEVLEYDKRGELNIVTICPSIIIGPLLQSTINASSLYLLKFLRGFSFLPLKIAFSFSKAEGSESEDNEVRPFLDVRDVADAILPVYKKTEANGRYICSSYEIGIRDLVEKLKIFYPNCNYPKRQREQTQLDAVKNYEESGVLTSHKKQPNVASKKCGYLEALSRYRVYWTPFDPNPSHRLHLLNVIG
MEKRNSKVCVTGGTGYFGSWLVKKLLEKGYTVHSTLRNLDKELLGYGSNVDGDSLEVITLAVGLVGGDSLLSYTPSTVAMYISQLTNSKYLYQALRFTEDLLGKIPMVHIDDACEAHIFCMEKPSINGRFLCASSYVSSAEIANYYQENYPEFHVKQEYLDGPKREIKWGGTKLMEKGFEYKYDTKMILDASIKCARRTGGLNQCKEYCFFALLLKFSWDSRSNKLIPMVLLKVPAVNMGSNR
>Sclbi147S07671_SCLBI
MAYLLSPPNPQRSVSETESSMCACACIYIYIYTGRRLRLHSKQAMDQIKGNVCVTGASGFLASWLVKRLLLAGYHVTGTVRDPGNEKKLAHLWRLEGAKERLRLVRANLMEEGSFDDAIKGCQGVFHTASPVVKPSFDPKACCVPTPSPVMLIDQDYLHIRALRYSYLASLACDIHHDQKPVNTNLLGIFPPTQAEILEPAVKGTLNVLRSCKKNPSLRRVVLTSSSSTVRARDDFDPKIPLDESSWSSVDLCEKFQIWYALSKTQAERAAWAFCEENRIDLVTVLPSFVVGPSLPPDLCSTASDVLGLLKGEAEKFQWHGRMGYVHIDDVALCHILVYEHKHCSGRYLCSSTVVDNNELASLLSSRYPWLRLQLRFEQLNRPYYEFNTSKLTSLGFKFKSIEVMFDDCIAWFVEQGYIRDKEQSYRNN
>Sclbi307S11192_SCLBI
MKSVLTYILYIIHRTHCKKCLALAAAELEEMEDLVSTVEGKREGDKSTYCVTGATGYIGSWLVKSLLERGCIVHATIRDPESYVQSNIIDPAIKGTLNLLRACLKSGSVKRVVFTSSISTITAKDNKGKWRPVVDESCQTPIDHVWNTKASGWVYVLSKLLTEEAAFKFAKENNIDLVSVITTTVAGPFLTSSVPSSIHVLLSPITGDSKFFSILTAVYTRMGSVALVHIEDVCNAHIFLMENAGAEGRFICCARSCSMSELVDHLNQEYPSSNKHSSIPSEISSKKLRDLGFTYNHGVEDIIRQTMASCLDCGLLSPIIK
>Sclbi1865S04675_SCLBI
MILDQPAELIDPALKGTLNVLGSCAKVQSIRRVVVTSSMASVYYNGNPLTTDVVVDETWFSNPALCKE
>Sclbi1865S09121_SCLBI
MLNFSVEVILNIINGAQTFPRPYRLVDVRDVAYVHIQALEVPTASGRYLLVERVAQLCEILKILREHYPTPHLPEKFKAAKYEPTDQISKERAKSLGTDFMPWGVSIRDTVESLKEKGFLNVYMHFGIRMHPFGIHLGSSISISSEINLILIVFQYWYHLSKTLAEEASWILVDDDGIDWVTLHPGSVIGPFLQSVLNFSVEGIENEWRGEGGVCNRSFRLHSFVAELIDPAVKGTLNVLRSSAKVESVKRVVVTSSIATVLFNGHSLTPDVVVDETWFSDPAFCTESKYWYLLSKTLAEEAACKFAKENGIELVTLHPGLVIGPFLQSTLNLSLELILDIINGAETWPSPFTVVDVRDVACAHIQALEVPTASGRYLLVERVSQLYEVLKILHENYPIFHLPEKFGDVKNEPTYQLSNERAKSLGVNFMPLEESLKDTVECFKEKGLLNLLLQRGYTVKATAELIDPAVKGTLNVLRSCAKFQSVKRVVVTSSLATVSFSGSPMTSDVVIDETWFSNSSFCKESKLWYHLAKTLAEEAAWKFAKENGIDLVPLHPGVVIGPFLQPKLNLSVAWILRLINGDQTLSSPFKLVDVRDVAFAHIQALEIPTASGRYLLVGSITHRSEIFEMLHQHYPTLQLPESGNNTNETTYQICKERAKSLGINFIPWEVSFRDTFENFKEKGFLNFTQIRRDRENEWRKEGGGCNRSFSCYSNVVTLSKPLFVTQAELIDPAVKGTLNVLRSCAKAQSVKKVVVTSSFASVFYNGNPLTPDVVVDETWFSNPTLCKESQNWYLLSKTLAEEAAWKFAKESGIDLVTLHPGFVIGPFLQSVPNFTVEVILNLTNGTQTYPSPYRLVDVRDVALAHIQALEVPTASGRYLLVGRVTQLCEILKTLHEHYPTLHLSEKFEASKCELTYQISQERAKSLGINFIPWEVSLRDTVESLKEKDDPKVQHLHALDGAKERLHFIFSPVFQAELIDPAVKGTLNVLRSCAKVQSVKRVVVTSSSTSVIFNGSPLTPDVVVDETWFSDPAFCKESKLWYALSKTLAEEAAWKFAKENAIDLVTLHPGFVIGPFLHSKLNASMEVILNMIKGVETGRGPHRFVDVRDVAYAHIQALEVPTSSGRYLLVGRVTQNCEVQKTLHEHYPTLHCPEKFEAANYEPTYQFSKEKAQSLGVSFMPWEVSLRDTVESLKEKGFLSVEKATEK
>Sclbi1865S04674_SCLBI
MWWVPDIEEYDPSYDVRWASEVTKCSSNEEVDNINLKSQRTIEDRLQMLEGASGYIASCLVKQLLEHDYTVKATVRDPNDSSKIKHLHALDGAKERLRIVKANLLEEGSFDSAVDGCEGVFHTASPVLLSSNEPQSVKRVIITSSMASVCFNERPLTPDVVIDETWFSDPTFCKESKIWYALSKTLAEEAAWKFAKENAIDLVTLHPGFVIGPFLQSMLNVSMEAILNIIKVAETFPSPYRFVDVRDVACAHIQALEIPAANGRYLLVGRVTKRCEVLKILYEHYPTLHLPEKFGAANYELTYQISKERAKSLGVNFMPLEVSLRNTVESFVEKDDSSRIKHLHELDGAKERLHLFNANLLKEGSFDSAVDGCEGVFPTASPVLLSSNEPQVNLILIYFQYWYHLSKTLAEEAAWSFAKENGTDLVTLHPGLSMPTFSVAVILNLVNGNNQQAFEDNECGRKTFPSPYRLVDVRDVAYARIQALEVPTASGRYWLVGRVTQLYEILKILHEHYPNFHLPEKFGDVKHEPVFQVCNERARSLGVNLMPWDVSLRDTLESLSRKASSVADKLLKGSGRSIVSIPDQSNKHGLFYDKLSCTSPFNHVKNICN
>Sclbi2838S10743_SCLBI
MALEKGRVCVTGAGGFLASWVVKLLLSKDYFVHGTVREPSDEKNAHLYKLDKASENLKLFKADLLDYDSLFSAISGCSGVFHVASPVPSIVVEVIEPAVKGTLNVLKACHEAKVKRVVYVSSVAAVGLNPSWPKDRLVDETSWSDKEYCRTTKNWYCLSKTEAEREALEYGKRTGLDVVSICPTLISGPLLQSTLNSSSLVLVKLVKEGYESVENKLRMIVDVRDVAEALLLAYEKPEAEGRYICTAHLIRAQDLVDKLKSIYPNYIYPKNFTDAGEERRPTTEKLQKLGWKMALEKGRVCVTGAGGFLASWVVKLLLSRHYFVHGTVQVIEPAVKGTLNVLKACVEAKIKRVVLVSSDAAIFMNPGWPKGQVMDETCWSDKEYCRTTKNWYYLSKTEAESEALEYAKRSGLDLVTICPTLILGPLLQSLVNASSLILIKLLKVLHIEGYESVENKLWRIVDVRDVAEALLLAYEKPEAEGRYICMAHMIETKDLVDKLKNMYPNYKYPKSFTEEGERIMLSSEKLQRLDLTKGNRKSNESLDWKGVTEMDVRVREEKERVCVTGAGGFIASWLVKFLLSKGYMVHGTNYYCLAKTMAESEALEYAEKGELDIVTVCPSIVLGPMLQTTINASSLLLLALLKGLFFHSVFQYTSFEGVEMTWKLDEHKSIEDKDRPLVDVRDVAEALLLVYEKPEAEGRYICTSYRIRMQALAEKLKSMYPNYSYPKSFTSDEDEVKLSSERLQKLGWKYRPLEETINKERVCVTGAGGYLASWVVKFLLSQAYLGHGTVRDLSDNKNAHLKKLEMASENLQLFKIDLRDNEGLCAAIDGCESVFHVACPVPPVGSVENPEVELLQPSVSGTRNVLNASLKAEVKEVWLCHLLGLLVKPELAQGSAHQWYCLAKTIAESEVLEYDKRGELNIVTICPSIIIGPLLQSTINASSLYLLKFLRGFSFLPLKIAFSFSKAEGSESEDNEVRPFLDVRDVADAILPVYKKTEANGRYICSSYEIGIRDLVEKLKIFYPNCNYPKRQREQTQLDAVKNYEESGVLTSHKKQPNVASKKCGYLEALSRYRVYWTPFDPNPSHRLHLLNVIG
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University