Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Sapindus mukorossi
Species name
Chinese soapberry (Sapindus mukorossi) is a species of eudicot in the family Sapindaceae (soapberry family).
Species info
Gene sequence info
Sapindus_mukorossi.COMT.fa
Download
>whz_003718-RA
MSSTHDDDDQHKPPKDEQNWLSAMQLVTGSVFPMVMKTAIELGLLKIMSKASPSQLSASQIASQLPTRNQEAPLIIERILRLLASHSILTCSLVTSEDGHVHRSYGLAPLCKYFVENEDGISLAPYLLVFQDKVSIDSWFHLKETILDGGFPFVKAHGMQVFEYAAIDGRMNQLFNEIMCNQTAFVMKKLLEVYKGFEELNQMVDIGGGLGSNLSFIVSKYPHIKGIYFDFPNAIKDAPYCPGVEHVSGDMFVEIPTGQAIFMKKNCYRALPKGGKVIVVESIVSAIPETDVVSQNICRLDMSLFTTVPGAKERTKEDFQALATAAGFAALKIICRVYCYWVIELHKDIYSPDVLN
>whz_009566-RA
MGSSETQMTPTQVSDEEANLFAMQITSGSVLPMVLKSAIELDLLEIIAKAGLGAHLSPTEIASQLPTTNPDAPVMLDRILRLLASYSILTCSIRTLPDGKVERLYGLAPVCKFLTKNEDGVSISALSLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFDYHGTDPRFNKVFNNGMSSHSTITMKKILETYKGFEGLKSIVDVGGGTGATVNMIVSQYPSIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSVPKADAIFMKWICHDWSDEHCLKFLKNCYEALPENGKVIVCECILPVAPDPSLGSKVVFHIDCIMLAHNPGGKERTEDDFRALAKGAGFQGFKVACCAFNTYIMEFLKKP
>whz_016717-RA
MAFAAEARANEVKEETFNYAIQLVDSVVLPMTLHVALELGVFEILAKAVRAEHREGAWLSAPNAVLHCYILQQIQSNAIYNAPNTTLMALTQDKVFLDSWSQLTSAVLEGGIPFNRVHGTQAYEYPGLDSRFNEVFNKAMHNHTTIVIKKILKVYKGFEKVKQLVDVGGRLGVTLRAVTAKYPHIESINFYLPHVIQHAPQYPDDGKVIVVEAILPVSPQTDVATKATSQIDVLMMTQCPGGKERARHEFMALATGAGFSGIRFECFACNFYVMELYK
>whz_016718-RA
MHNNILFYKTAFCVIGIEHVEGDMFESIPKGDAILMKWILHDWSDEHCLKLLKNCYKAIPDDGKLIVIEAITPVLPETDAATKSTFQIDVLMMTQNPGGKERSRPEFMALAIGAGFSDIKFVTCACNFWVMEFYK
>whz_022050-RA
MDSTYVSYHDQEADYLHAMELASGSVLPMVLKSAIELDLLEIIARAGPGASMSPHEIASHLPTNNPDAPSMLDRIFRLLASYSILTCSLRSLPAGKVERLYGLAPICKYFTKSEDGVSLSAYSLWSHDKVTMEAWHYLKDAVLEGGIPFNKAHGMPIFQYLGKNPRTNKIFNNAMSSWSTLNEKKILENYKGFEGLKSIVDVGGGTGVTLSMIVAKFPSIKGINFDLTHVIQGAPSYPGVVHVEGDMFASVPKGDAILLKRVCHDWSDEHCSKLLRNCYEALPKEGKIIVCDYILPVAPEQNLGSKLAFHMDCTMMSLSQFGKERTKDEFEALAKEAGFQGFQLVCCVPTIYIMEFLKKA
>whz_006614-RA
MENEGKESRNEARLAIMELANMISVPMSLNAIVQLNVADAIWQGGSNIPLSASQILSRALHSGTPADPQNLQRILRMLTSYGVFAEHLDPSDSSLRKYSLTEIGQTLVTDSEGISYAPYVLQHHQEALMKAWPLVHEAVLDTTSEPFVKANGEPAYSYYGKRPEMNGLMQKAMSGVSVPFVKAILDGYDGFEGVERLVDVGGSAGDCLRMILQKHPNVREGINFDLPEVVAKAPDIPGVTHVGGDMFKSIPAGDAIFMKWILTTWTDDECKLIMENCCKALPAGGKLIACEPVLPNESDDSHRTRALLEGDIFVMTIYRAKGKHRTEEEFKKLGLAAGFPNFRAIYIDYFYTVLEFQK
MSSTHDDDDQHKPPKDEQNWLSAMQLVTGSVFPMVMKTAIELGLLKIMSKASPSQLSASQIASQLPTRNQEAPLIIERILRLLASHSILTCSLVTSEDGHVHRSYGLAPLCKYFVENEDGISLAPYLLVFQDKVSIDSWFHLKETILDGGFPFVKAHGMQVFEYAAIDGRMNQLFNEIMCNQTAFVMKKLLEVYKGFEELNQMVDIGGGLGSNLSFIVSKYPHIKGIYFDFPNAIKDAPYCPGVEHVSGDMFVEIPTGQAIFMKKNCYRALPKGGKVIVVESIVSAIPETDVVSQNICRLDMSLFTTVPGAKERTKEDFQALATAAGFAALKIICRVYCYWVIELHKDIYSPDVLN
>whz_009566-RA
MGSSETQMTPTQVSDEEANLFAMQITSGSVLPMVLKSAIELDLLEIIAKAGLGAHLSPTEIASQLPTTNPDAPVMLDRILRLLASYSILTCSIRTLPDGKVERLYGLAPVCKFLTKNEDGVSISALSLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFDYHGTDPRFNKVFNNGMSSHSTITMKKILETYKGFEGLKSIVDVGGGTGATVNMIVSQYPSIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSVPKADAIFMKWICHDWSDEHCLKFLKNCYEALPENGKVIVCECILPVAPDPSLGSKVVFHIDCIMLAHNPGGKERTEDDFRALAKGAGFQGFKVACCAFNTYIMEFLKKP
>whz_016717-RA
MAFAAEARANEVKEETFNYAIQLVDSVVLPMTLHVALELGVFEILAKAVRAEHREGAWLSAPNAVLHCYILQQIQSNAIYNAPNTTLMALTQDKVFLDSWSQLTSAVLEGGIPFNRVHGTQAYEYPGLDSRFNEVFNKAMHNHTTIVIKKILKVYKGFEKVKQLVDVGGRLGVTLRAVTAKYPHIESINFYLPHVIQHAPQYPDDGKVIVVEAILPVSPQTDVATKATSQIDVLMMTQCPGGKERARHEFMALATGAGFSGIRFECFACNFYVMELYK
>whz_016718-RA
MHNNILFYKTAFCVIGIEHVEGDMFESIPKGDAILMKWILHDWSDEHCLKLLKNCYKAIPDDGKLIVIEAITPVLPETDAATKSTFQIDVLMMTQNPGGKERSRPEFMALAIGAGFSDIKFVTCACNFWVMEFYK
>whz_022050-RA
MDSTYVSYHDQEADYLHAMELASGSVLPMVLKSAIELDLLEIIARAGPGASMSPHEIASHLPTNNPDAPSMLDRIFRLLASYSILTCSLRSLPAGKVERLYGLAPICKYFTKSEDGVSLSAYSLWSHDKVTMEAWHYLKDAVLEGGIPFNKAHGMPIFQYLGKNPRTNKIFNNAMSSWSTLNEKKILENYKGFEGLKSIVDVGGGTGVTLSMIVAKFPSIKGINFDLTHVIQGAPSYPGVVHVEGDMFASVPKGDAILLKRVCHDWSDEHCSKLLRNCYEALPKEGKIIVCDYILPVAPEQNLGSKLAFHMDCTMMSLSQFGKERTKDEFEALAKEAGFQGFQLVCCVPTIYIMEFLKKA
>whz_006614-RA
MENEGKESRNEARLAIMELANMISVPMSLNAIVQLNVADAIWQGGSNIPLSASQILSRALHSGTPADPQNLQRILRMLTSYGVFAEHLDPSDSSLRKYSLTEIGQTLVTDSEGISYAPYVLQHHQEALMKAWPLVHEAVLDTTSEPFVKANGEPAYSYYGKRPEMNGLMQKAMSGVSVPFVKAILDGYDGFEGVERLVDVGGSAGDCLRMILQKHPNVREGINFDLPEVVAKAPDIPGVTHVGGDMFKSIPAGDAIFMKWILTTWTDDECKLIMENCCKALPAGGKLIACEPVLPNESDDSHRTRALLEGDIFVMTIYRAKGKHRTEEEFKKLGLAAGFPNFRAIYIDYFYTVLEFQK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University