Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Sapindus mukorossi
Species name
Chinese soapberry (Sapindus mukorossi) is a species of eudicot in the family Sapindaceae (soapberry family).
Species info
Gene sequence info
Sapindus_mukorossi.4CL.fa
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>whz_001496-RA
MEKSGYGRDGIYRSLRPPLVLPKDPNLSMVSFLFRNSSSHDSKLALIDADSSSSLTYSQLKSTLIKVAHGFRHLGVTRNDVVLIFAPNSIQFPICFLGVIALGAIATTANPVYTVSELAKQVKDCNPKVVVTVPELYDKVKDFNLTTVVLGSKEKLSSFVVTPSSKAKVVHFHDLVELSGDVSEFPNVSIKQSDTASLLYSSGTTGVSKGVILTHGNYIAASLMITMDQDLAGELHGVYLCVLPMFHVFGLAVLVYSQLQKGNTIVSMAKFEFEMALRAIEKYRVTYLWVVPPIVLALAKHSLVKKFDLSSLKHIGSGAAPLGKDLMEECSRNIPRVIVLQGYGMTETSGIISVEDPRSEIRNTGSAGFLAAGVEAQIVSVDTQKPLPPTQMGEIWVRGPNMMQGYFNNKQATMQTIDKQRWVHTGDLGYFDENGHLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPYPDVEAGEVPVAYVVRSPTSSLTEEEVQNFIAKQVAPFKRLRRVTFINSVPKSASGKILRRELIAKVRSKI
>whz_003179-RA
MISIADHHPVEPQKTDQLSSSNTSSNNSNITTHVFKSKLPDIPISNHLPLHTYCFQNVSDFPDNPCLIVGSTAKTYSFAETLQICRKTAAGLSNLGIKQGDVIMILLQNCAEFVFAFMGASMIGAVTTTANPFYTCSEIFKQLNTSRAKLIITVSQYVDKLRDSTDENHPKIGQDFTIITIDEPPENCLHFTVLSEANENDIPEVTINPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLSLIHQDVVLCVLPLFHIFSLNSVLLCSLRAGAGVLLMQKFEIGTLLELIQKHKVSVAAVVPPLVLALAKNPKVAEFDLSSIRMVLSGAAPLGKELEEALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPYPTKSGSCGTVVRNAQLKVIDPETGCSLTYNQPGEICIRGPQIMKGYLNDPEATASTIDLDGWLHTGDIGYVDDDDEVFIIDRVPPAELEALLINHPSIADAAVVPQKDDVAGEVPVAFVVRSNGFELTEEAIKEYIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLASAASPTVC
>whz_013217-RA
MSPTDPDNSISEITHEIQLSRPSVAFATSHTSYKLPSNLRRILIDSPEFVSLLTRDTDCGGNASCIIDNGEVVVNQSDVAAIMYSSGTTGRVKGVPLTHRNLIATIACSYNQKIRTATQQTVSLTTRVFFHLFGFAMMMRAISHGEALVILETFELEAILEAIEKFKATYAPISPNYIVALLKSETTNKYNLTSLRYFVCAGASLGMEVAHKFKEKFPNIAIVTGYGMTETASAGAFMREPKERERVGSVGRLANFMEAKIVDVVTGEALPPGGKGELWLRGPCIMRGYVRDETTTAQVLDSHGWLKTGDLCYFDSHGFLYIVDRLKELIKYNGFQVAPAELEHLLQANSNIADAAVVGYPDEEAGQIPIAFVVKKPGTAITGAQIMDFIADQVAPHRRIRRVTFIDSIPRSVQGKILRKELIARALSSN
>whz_014727-RA
MFLTIVWLMVLQVCSCIFHFYLASPHCTATTLVYASYHSVCTPEGPFMATTNTNTNSKIDPNSGFCSETKIFHSLRPSISLPPPSQPLSITQFILTLLHSTSTNFSETPFLINDVTGHRITYSEFLHQTKSLACSLQKQYSLSKGNVAFILCPSSLNVPVLYLSLLSLGVIIAPANPDDSISELTHQIQLSRPTIVFASSHTSHKLPPTLQPTILIDSPQFSDLLIDQHSNINISSDVVNQSDIATICFSSGTTGRNKGVLLTHGNLIWCTANFPAIVPVKQRVAMIRRPLYSAYLIAYMLKAASIGETLVLTQTSDFETALRAVQRYKLNHFTAPPSFIVEMMKSDITKHDLSSLRFVVTTGAPLGKEVSSKFKENFPNVELLDQYGSSEAGTLAAMRSPNEINKVGSVGRLVEHVEAKIVDPLTGEVLSPGQKGELWVRAPTVMKGYVGDEKATAEALDSEGWLKTGDVGLFDSDGFLFIVDRLKEIFKCNGCQVAPAELEHLLLSNPDISDAAVVPYSDEVAGQIPIAFVVKKPGTTITEAEVMDFIAKQV
>whz_014726-RA
METSYGCTANFPAIVPVKQRVAMIRRPLYSAYLIAYMLKAASIGETLVLTQTSDFETALRAVQRYKLNHFTAPPSFIVEMMKSDITKHDLSSLRFVVTTGAPLGKEVSSKFKENFPNVELLDQYGSSEAGTLAAMRSPNEINKVGSVGRLVEHVEAKIVDPLTGEVLSPGQKGELWVRAPLS
>whz_025929-RA
MEAPAHNEEHIFRSRHRGVPIPDVTLPEFVLQDVELYGDKVAFVEAVTGKAYTYSEVARDIKRFAKALRSLGLRKGRVVVVVLPNVAEYGIVALGIMAAGGVFSGANPTAHSSEIKKQVEAADAKLIVTSGPIYEKVKGLGLPVIVLGEERIESAMNWKELLEAADRAGNNFTNNEIHQTDLCALPFSSGTTGMSKGVMLTHRNLVANLCSTLFSVGPEMIGQVTTLGLIPFFHIYGITGICCATLRNKGKVVVMNRFELRTFLNAFITQEVTFAPIVPPIILALVKNPIVEEFDLTKLKLRAVMTAAAPLAPELLAAFEKKFPTVQVQEAYGLTEHSCITLTHGDPTKPNGSNKNSVGYILPNLEVKFIDPETGLSLPKNTPGEICVRSQCVMQGYFNNEEETARTIDKNGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLSHPSVEDAAVVPLPDEEAGEIPAACVVINPNSKETEEDLMNYVASNVATYKRVRALQFVRTIPKSPSGKIMRRLLREKMIENTQPKAKANNNA
>whz_017916-RA
MAPELINQEEDIIFRSKLPDIYIPNHLPLHTYVFQNLSKLSSRPCLINGSTGHIYTYGEVELIARKVASGFKKIGIEQHQVIMILLPNCPEFVFSFLGASYLGAIATMANPFFTPAEISKQAEASNARVLITQACYVDKVKDFAERKNVQVVCIDTAPEGCLHFSDLSQADETKLPEVDIHPDDAVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHNEDVILCVLPMFHVYALNSIMLCGLRVGATILIMQKFEINSLLQLVEKHKVTVAPMVPPIVLSIAKSPDTVKYDLSSVRIVKSGAAPMGKELEDSVRAKFPNAVLGQGYGMTEAGPVLAMCLSFAKEPYSIKPGACGTVVRNAEMKIVDPETGKSLPRNQPGEICIRGAQIMKGYLNDPEATKNTIDQDGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLINHPDIRDAAVVAMKDDAAGEVPVAFVVRTNDSQITEDEIKQFISKQVIFYKRINRVFFIDAIPKAPSGKILRKDLRAKLAA
>whz_021319-RA
MEAQEDQQEFIFRSKLPDIYIPNHLPLHTYCFENISQFKDRPCIINGPTGDIYTYADVELTSRKVAAGLDKFGIKQGDVIMLLLQNCPQFVFAFLGASYIGAISTTANPFYTPAEIAKQATASKAKLIITQVLYAEKVKDFALGSGVKVMTVDSTSPDHEGNENYFFHFSELTKADENDSPAVKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHKEDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIITLMELVQKYKVTIAPFVPPIVLAIAKSPDINKYDLSSIRTVMSGAAPMGKELEDSVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPDSGASLPRNQSGEICIRGSQIMKGYLNDEEATERTVDKQGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHANISDAAVIPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVVFYKRINRVFFTNTIPKAPSGKILRKDLRAKLAAGLPN
MEKSGYGRDGIYRSLRPPLVLPKDPNLSMVSFLFRNSSSHDSKLALIDADSSSSLTYSQLKSTLIKVAHGFRHLGVTRNDVVLIFAPNSIQFPICFLGVIALGAIATTANPVYTVSELAKQVKDCNPKVVVTVPELYDKVKDFNLTTVVLGSKEKLSSFVVTPSSKAKVVHFHDLVELSGDVSEFPNVSIKQSDTASLLYSSGTTGVSKGVILTHGNYIAASLMITMDQDLAGELHGVYLCVLPMFHVFGLAVLVYSQLQKGNTIVSMAKFEFEMALRAIEKYRVTYLWVVPPIVLALAKHSLVKKFDLSSLKHIGSGAAPLGKDLMEECSRNIPRVIVLQGYGMTETSGIISVEDPRSEIRNTGSAGFLAAGVEAQIVSVDTQKPLPPTQMGEIWVRGPNMMQGYFNNKQATMQTIDKQRWVHTGDLGYFDENGHLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPYPDVEAGEVPVAYVVRSPTSSLTEEEVQNFIAKQVAPFKRLRRVTFINSVPKSASGKILRRELIAKVRSKI
>whz_003179-RA
MISIADHHPVEPQKTDQLSSSNTSSNNSNITTHVFKSKLPDIPISNHLPLHTYCFQNVSDFPDNPCLIVGSTAKTYSFAETLQICRKTAAGLSNLGIKQGDVIMILLQNCAEFVFAFMGASMIGAVTTTANPFYTCSEIFKQLNTSRAKLIITVSQYVDKLRDSTDENHPKIGQDFTIITIDEPPENCLHFTVLSEANENDIPEVTINPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLSLIHQDVVLCVLPLFHIFSLNSVLLCSLRAGAGVLLMQKFEIGTLLELIQKHKVSVAAVVPPLVLALAKNPKVAEFDLSSIRMVLSGAAPLGKELEEALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPYPTKSGSCGTVVRNAQLKVIDPETGCSLTYNQPGEICIRGPQIMKGYLNDPEATASTIDLDGWLHTGDIGYVDDDDEVFIIDRVPPAELEALLINHPSIADAAVVPQKDDVAGEVPVAFVVRSNGFELTEEAIKEYIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLASAASPTVC
>whz_013217-RA
MSPTDPDNSISEITHEIQLSRPSVAFATSHTSYKLPSNLRRILIDSPEFVSLLTRDTDCGGNASCIIDNGEVVVNQSDVAAIMYSSGTTGRVKGVPLTHRNLIATIACSYNQKIRTATQQTVSLTTRVFFHLFGFAMMMRAISHGEALVILETFELEAILEAIEKFKATYAPISPNYIVALLKSETTNKYNLTSLRYFVCAGASLGMEVAHKFKEKFPNIAIVTGYGMTETASAGAFMREPKERERVGSVGRLANFMEAKIVDVVTGEALPPGGKGELWLRGPCIMRGYVRDETTTAQVLDSHGWLKTGDLCYFDSHGFLYIVDRLKELIKYNGFQVAPAELEHLLQANSNIADAAVVGYPDEEAGQIPIAFVVKKPGTAITGAQIMDFIADQVAPHRRIRRVTFIDSIPRSVQGKILRKELIARALSSN
>whz_014727-RA
MFLTIVWLMVLQVCSCIFHFYLASPHCTATTLVYASYHSVCTPEGPFMATTNTNTNSKIDPNSGFCSETKIFHSLRPSISLPPPSQPLSITQFILTLLHSTSTNFSETPFLINDVTGHRITYSEFLHQTKSLACSLQKQYSLSKGNVAFILCPSSLNVPVLYLSLLSLGVIIAPANPDDSISELTHQIQLSRPTIVFASSHTSHKLPPTLQPTILIDSPQFSDLLIDQHSNINISSDVVNQSDIATICFSSGTTGRNKGVLLTHGNLIWCTANFPAIVPVKQRVAMIRRPLYSAYLIAYMLKAASIGETLVLTQTSDFETALRAVQRYKLNHFTAPPSFIVEMMKSDITKHDLSSLRFVVTTGAPLGKEVSSKFKENFPNVELLDQYGSSEAGTLAAMRSPNEINKVGSVGRLVEHVEAKIVDPLTGEVLSPGQKGELWVRAPTVMKGYVGDEKATAEALDSEGWLKTGDVGLFDSDGFLFIVDRLKEIFKCNGCQVAPAELEHLLLSNPDISDAAVVPYSDEVAGQIPIAFVVKKPGTTITEAEVMDFIAKQV
>whz_014726-RA
METSYGCTANFPAIVPVKQRVAMIRRPLYSAYLIAYMLKAASIGETLVLTQTSDFETALRAVQRYKLNHFTAPPSFIVEMMKSDITKHDLSSLRFVVTTGAPLGKEVSSKFKENFPNVELLDQYGSSEAGTLAAMRSPNEINKVGSVGRLVEHVEAKIVDPLTGEVLSPGQKGELWVRAPLS
>whz_025929-RA
MEAPAHNEEHIFRSRHRGVPIPDVTLPEFVLQDVELYGDKVAFVEAVTGKAYTYSEVARDIKRFAKALRSLGLRKGRVVVVVLPNVAEYGIVALGIMAAGGVFSGANPTAHSSEIKKQVEAADAKLIVTSGPIYEKVKGLGLPVIVLGEERIESAMNWKELLEAADRAGNNFTNNEIHQTDLCALPFSSGTTGMSKGVMLTHRNLVANLCSTLFSVGPEMIGQVTTLGLIPFFHIYGITGICCATLRNKGKVVVMNRFELRTFLNAFITQEVTFAPIVPPIILALVKNPIVEEFDLTKLKLRAVMTAAAPLAPELLAAFEKKFPTVQVQEAYGLTEHSCITLTHGDPTKPNGSNKNSVGYILPNLEVKFIDPETGLSLPKNTPGEICVRSQCVMQGYFNNEEETARTIDKNGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLSHPSVEDAAVVPLPDEEAGEIPAACVVINPNSKETEEDLMNYVASNVATYKRVRALQFVRTIPKSPSGKIMRRLLREKMIENTQPKAKANNNA
>whz_017916-RA
MAPELINQEEDIIFRSKLPDIYIPNHLPLHTYVFQNLSKLSSRPCLINGSTGHIYTYGEVELIARKVASGFKKIGIEQHQVIMILLPNCPEFVFSFLGASYLGAIATMANPFFTPAEISKQAEASNARVLITQACYVDKVKDFAERKNVQVVCIDTAPEGCLHFSDLSQADETKLPEVDIHPDDAVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHNEDVILCVLPMFHVYALNSIMLCGLRVGATILIMQKFEINSLLQLVEKHKVTVAPMVPPIVLSIAKSPDTVKYDLSSVRIVKSGAAPMGKELEDSVRAKFPNAVLGQGYGMTEAGPVLAMCLSFAKEPYSIKPGACGTVVRNAEMKIVDPETGKSLPRNQPGEICIRGAQIMKGYLNDPEATKNTIDQDGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLINHPDIRDAAVVAMKDDAAGEVPVAFVVRTNDSQITEDEIKQFISKQVIFYKRINRVFFIDAIPKAPSGKILRKDLRAKLAA
>whz_021319-RA
MEAQEDQQEFIFRSKLPDIYIPNHLPLHTYCFENISQFKDRPCIINGPTGDIYTYADVELTSRKVAAGLDKFGIKQGDVIMLLLQNCPQFVFAFLGASYIGAISTTANPFYTPAEIAKQATASKAKLIITQVLYAEKVKDFALGSGVKVMTVDSTSPDHEGNENYFFHFSELTKADENDSPAVKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHKEDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIITLMELVQKYKVTIAPFVPPIVLAIAKSPDINKYDLSSIRTVMSGAAPMGKELEDSVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPDSGASLPRNQSGEICIRGSQIMKGYLNDEEATERTVDKQGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHANISDAAVIPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVVFYKRINRVFFTNTIPKAPSGKILRKDLRAKLAAGLPN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University