Enzyme
The enzyme catalyses the synthesis of catechin, catechin-4beta-ol (leucocyanidin) and the related flavan-3-ols afzelechin and gallocatechin, which are initiating monomers in the synthesis of plant polymeric proanthocyanidins or condensed tannins. While 2,3-trans-3,4-cis-leucocyanidin is the preferred flavan-3,4-diol substrate, 2,3-trans-3,4-cis-leucodelphinidin and 2,3-trans-3,4-cis-leucopelargonidin can also act as substrates, but more slowly. NADH can replace NADPH but is oxidized more slowly.
LAR1
Enzyme
Leucoanthocyanidin reductase
Enzyme name
EC:1.17.1.3
Entry
Oxidoreductases;Acting on CH or CH2 groups;With NAD+ or NADP+ as acceptor
Class
(2R,3S)-catechin:NADP+ 4-oxidoreductase
Sysname
(2R,3S)-catechin + NADP+ + H2O = 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ [RN:R06532]
Reaction
2,3-trans-3,4-cis-leucocyanidin [CPD:C05906];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Salix purpurea
Species name
Purple osier (Salix purpurea) is a species of eudicot in the family Salicaceae (willow family).
Species info
Gene sequence info
Salix_purpurea.LAR1.fa
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>Sapur.008G093600.1.v5.1
MTVSASTPTTKSRVLIAGATGFIGQFVASASLDSGRQTYVLVRPGLAGCLSKSKVVKSLHDKGAIILHGSISDRENMAKILKDHEIDIVISAVGGANVLDQIGLVEAIKAVGTIKRFLPSEFGHDVVRAVPVEPGEQMYKDKRVIRELVEKYSIPYNYICCNSIASWPYYDNKHPANVLPPLDHFKIYGDGTVKAYFVAGTDIGKFTMKIVDDVRAINKSVHFRPSSNFYNMNELASLWEKKIGRTLPRATVTEQDLLDIAAENIIPESIVASFTHDIFIKGCQSNFSINGPDDIEVGTLYPDEAFRTVDECFNDFALELKDTAMDLGSKANAAPNHAVESMALSAPCALSV
>Sapur.010G100300.1.v5.1
MTVSAAPSTVKSRVLIAGATGFIGQFVAKASLDSGRPTYVLVRPGAAASPSKAEVLKSLHDKGAIILQGLVSDRENMEKLLKVHEIDIVISALGGENVLDQLGLIEAIKAVGTIKRFLPSEFGHDVARADPVEPGLHMYKEKREIRRFVEEYGIPYTYICCNSIASWPYYDNKHPADALPPLDRFKIYGDGTVKAYFVAGTDIGKFTMKTVDDARTLNKSVHFRPPSNFYNMNELASLWEKKIGRTLPRVTVTENDLLAIAADNIMPDSIVAAFTHDIFIKGCQFNFSISSPEDVDVGTLYPGESFRTLDECFNDFALKLKGNNKDAGGHGNATPNHVVESLAISSSCVACDKKISLFAGM
>Sapur.15ZG057000.1.v5.1
MNGDSPNVWEGGQVMIIGSVGFNGGFIAEVSLECGRPTYLLIRSELASLCKASAIRFLEDKRASTMYGSIEDEDLKKVVREHKIEMVISAVGGASIADQVKLVNAIEAAGTVKFGHDIDKADPVEPGLTMYEENRQLPWPYHDNTHHADALPPLDRLQVYAYFVAGSDIGKLAIKSIVDNRTLNKTVHFRPPSSLLSTNELASLWEEKLGYKPPRVTITEGDLLADARGMRIPQSIVAAITHDIFIKSCQINYSLEQPNDVEVCSLYPELPFRTLDE
MTVSASTPTTKSRVLIAGATGFIGQFVASASLDSGRQTYVLVRPGLAGCLSKSKVVKSLHDKGAIILHGSISDRENMAKILKDHEIDIVISAVGGANVLDQIGLVEAIKAVGTIKRFLPSEFGHDVVRAVPVEPGEQMYKDKRVIRELVEKYSIPYNYICCNSIASWPYYDNKHPANVLPPLDHFKIYGDGTVKAYFVAGTDIGKFTMKIVDDVRAINKSVHFRPSSNFYNMNELASLWEKKIGRTLPRATVTEQDLLDIAAENIIPESIVASFTHDIFIKGCQSNFSINGPDDIEVGTLYPDEAFRTVDECFNDFALELKDTAMDLGSKANAAPNHAVESMALSAPCALSV
>Sapur.010G100300.1.v5.1
MTVSAAPSTVKSRVLIAGATGFIGQFVAKASLDSGRPTYVLVRPGAAASPSKAEVLKSLHDKGAIILQGLVSDRENMEKLLKVHEIDIVISALGGENVLDQLGLIEAIKAVGTIKRFLPSEFGHDVARADPVEPGLHMYKEKREIRRFVEEYGIPYTYICCNSIASWPYYDNKHPADALPPLDRFKIYGDGTVKAYFVAGTDIGKFTMKTVDDARTLNKSVHFRPPSNFYNMNELASLWEKKIGRTLPRVTVTENDLLAIAADNIMPDSIVAAFTHDIFIKGCQFNFSISSPEDVDVGTLYPGESFRTLDECFNDFALKLKGNNKDAGGHGNATPNHVVESLAISSSCVACDKKISLFAGM
>Sapur.15ZG057000.1.v5.1
MNGDSPNVWEGGQVMIIGSVGFNGGFIAEVSLECGRPTYLLIRSELASLCKASAIRFLEDKRASTMYGSIEDEDLKKVVREHKIEMVISAVGGASIADQVKLVNAIEAAGTVKFGHDIDKADPVEPGLTMYEENRQLPWPYHDNTHHADALPPLDRLQVYAYFVAGSDIGKLAIKSIVDNRTLNKTVHFRPPSSLLSTNELASLWEEKLGYKPPRVTITEGDLLADARGMRIPQSIVAAITHDIFIKSCQINYSLEQPNDVEVCSLYPELPFRTLDE
Sheng jun & Dong yang Teams, Yunnan agriculture University