Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Salix purpurea
Species name
Purple osier (Salix purpurea) is a species of eudicot in the family Salicaceae (willow family).
Species info
Gene sequence info
Salix_purpurea.COMT.fa
Download
>Sapur.002G137700.1.v5.1
MASAIENRSSQVDEANDEDFGYAMQLALGSVLPMTLYSAIQLGIFEIIAKAGPDARLSASDIAVKLPTKNPDAPKMLDRILRLLASHQVLCCSVNGLERLYSLAPVSNYFVRNQNGVSLAPFMALLQDSVFLQSWSQLKDSVLEGGVAFDRVHGAHAFEYPGLDPRFNQVFYAAMYNQTNLVIGNMLETYTGFNDLKQLVDIGGGLGHTIKAITSKYPQIKGINFDLPHVIEHAPAYPGVEHVGGDMFECVPKGDAIFLKWILHDWSDDHCLKLLKNCYKATPDDGKVIVMESVLPTTTKTSAAAKAASQLDVLMMTQNPGGKERTEDEFIALAAGAGFRGIKFETFVHNFWVMEFFK
>Sapur.006G098600.1.v5.1
MALNDESAEKRKSARLAILELANMISVPMSLNAVVRLNVADAIWQGGSNTPLSASQILQRIASTGSDPENLQRILRMLTSYGVFEEHLTNESSPLDGSASERKYSLTEIGKTLVTDADGLSYAPYVLQHHQDALMKAWPLVHEAVLDPTTEAFVKANGEPAYDYYGKQEEMNELMLKAMSGVSVPFMKAMLDGYDGFKGVERLVDVGGSAGDCLRMILKKYPGVRHGINFDLPEVVAKAPNIPGVTHVGGDMFKSIPDGDAIFMKWILTTWTDNECKLIMENCYKALPEGGKLIACEPVLPKDSDDSHRTRALLEGDIFVMTIYRAKGKHRTGEEFMQLGQSVGFSHFRAFHVDYFYTVLEFQK
>Sapur.012G002900.1.v5.1
MGSTGDQTQMTPTQVSDEEAHLFAMQLASASVLPMILKTAIELDLLEIMAKAGPGAFLSSSEIASHLPTKNPDAPVMLDRILRLLASYSILTCSLKDLPDGKVERLYGVAPVCKFLTKNEDGVSVSPLCLMNQDKVLMESWYYLKDAILDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTITMKKILETYKGFEGLKSLVDVGGGTGAVVNTIVSKYPSIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSIPKADAVFMKWICHDWSDEHCLSFLKKCYDALPENGKVILVECILPVAPDTSLATKGVVHVDVIMLAHNPGGKERTEKEFEGLAKGAGFQGFEVMCCAFNTYVIEFRKKA
>Sapur.013G111700.1.v5.1
MTLSDLVSSLPIHPSKTQYVHRIMHILVHSGFFSQQNNDQEIYSLSQSGRLLVEDNPLSIRSLVLMLLDPVLTKPWDCLSTWFRNDEVAAFSVAHGKTIWEYTDQDPRLNNLFNAAMASDSILVSKVLVSKCKGIFEGVNFLVDVGGGIGTMAKAISEAFPHIHCTVFDLPHAVSDLQGSKNLEYVVGDMFEAVPSADTLLLKWILHDWSDEDCIKIIP
>Sapur.014G084100.1.v5.1
MGRKNQKQKNQKHRVSQSMPMAASVTEEEATQNDNQKQTVEEERESFTNAMTLVNASVLPLALKTVIDLGVLEVLATADPDVGLTAAEIAKRIPALNPEAPVMLERILRLLMSEGVVYCSHALFDEAPMKYRLGRVGKYFVRDDSGVSLAPLMALAHDKVYLETWSNLKDAILEGRTPFNRAHGTNLFEYSARDARFSQVYNTAMFNHTNLVFKKILESYSGFENLKQVVDVGGGIGVALSLIAFKYPFINAINFDLPHVIQHAPPFPGVKHVEGDMFKSVPKGDAIILKWILHDWDDEHCLTLLKNCYLSVPADGKIIVVEQILPTFAEITAVSRDKSQHDMISLTQTPGGKERMQNQLFNLAFSAGFKSVRLVSYVYHYWVMEFMK
>Sapur.014G084300.1.v5.1
MGSLKESHVGHVAEAKDENFGYAMQLALSSVLPMTLFSAIELGVFEIIAKAGPDAKLSASDVAAQLPTTNPDAPMMLDRILRLLASHDVLACSVDGLERLYSLAPVSKHYVRNEDGVSLGPFMALIQDNVFLKSWSQLKDSVLEGGVAFDRVHGVHSFEYTGLDPRFNQVLNTAMYNQTTIILENMLEAYSGFKNLKTLVEIGGGMGHTIKAIISKYPQIKGINFDLPHVIEHAPPCRGVEHVGGDMFGSVPKGDAIFLKWILHDWSDDRCLKLLKNCYKAIPDNGKVIVMESVLSITAKSSPAARAISQLDVLMMTQNPGGKERTEHEFMALATGAGFRGIRYEAFACNFWVMEFFK
>Sapur.15WG002100.1.v5.1
MGSTGEAQMSPAQALNEDANCALLLVSSSVLPMVLKTAVELDLLEIMAKAGPGASMSPSDIASHLPTKNPDAPSMLDRILRLLASYSILTCSLRDLPDGKVERLYGLASVCKFLTKNEDGGSLGPLCLMNQDKVLMESWYHLKDAILDGGVPFNKAYGMTAFEYHGKDPRINKVFNKGMSDHSTITMKSILETYRGFEGIASLVDVGGGTGAVISAIVSKYPSIKGINFDLPHVIADAPSFPGVENVGGDMFVSVPKADAVFMKWICHDWSDEHCFKLLKNCYDALPENGKVILVDCIIPVVPDTSLATKGVIHADVIMLAHNPGGKERTEKEFESLAKGCGFKGLEVMCCVFNTYVIEFRKQL
>Sapur.15ZG002100.1.v5.1
MGSTGEAQMSPAQALNEDANCALLLVSSSVLPMVLKTAVELDLLEIMAKAGPGASMSPSDIASHLPTKNPDAPSMLDRILRLLASYSILTCSLRDLPDGKVERLYGLASVCKFLTKNEDGGSLGPLCLMNQDKVLMESWYHLKDAILDGGVPFNKAYGMTAFEYHGKDPRINKVFNKGMSDHSTITMKSILETYRGFEGIASLVDVGGGTGAVISAIVSKYPSIKGINFDLPHVIADAPSFPGVENVGGDMFVSVPKADAVFMKWICHDWSDEHCFKLLKNCYDALPENGKVILVDCIIPVVPDTSLATKGVIHADVIMLAHNPGGKERTEKEFESLAKGCGFKGLEVMCCVFNTYVIEFRKQL
>Sapur.016G291000.1.v5.1
MATSSDEEDYHLQYALQLASASTLPMVFKAVIELGVLEIIEKAGPAAMLSASQIASQLPTQNNPDAHIVLDRILSLLASHSILTCSLATENQDSDQIQRLYGLAPVAKYFIRKEDGGSLSPYFRVIHDKVIMDLWYHLKDVVLQGGALFQKAYGMSSMEYVKKDPRFGEVFGGFVRGFNPLFMKRILDIYDGFEGLTSLVDVGGGDGSVLNMIVSKYPAIKGINFDLASVIENSPSYPGIEHVAGDVFLTIPEGEAIFMKWVSHFFSDDNFLKVLKNCYGALPDNGKLIVVEMVIPEIPGTSAADRSLMQNYLFVTSMNPKRNERTEKDFERLAKVAGFSHFRVACSACTFSVVEFVKNKATAGAVAIAAK
MASAIENRSSQVDEANDEDFGYAMQLALGSVLPMTLYSAIQLGIFEIIAKAGPDARLSASDIAVKLPTKNPDAPKMLDRILRLLASHQVLCCSVNGLERLYSLAPVSNYFVRNQNGVSLAPFMALLQDSVFLQSWSQLKDSVLEGGVAFDRVHGAHAFEYPGLDPRFNQVFYAAMYNQTNLVIGNMLETYTGFNDLKQLVDIGGGLGHTIKAITSKYPQIKGINFDLPHVIEHAPAYPGVEHVGGDMFECVPKGDAIFLKWILHDWSDDHCLKLLKNCYKATPDDGKVIVMESVLPTTTKTSAAAKAASQLDVLMMTQNPGGKERTEDEFIALAAGAGFRGIKFETFVHNFWVMEFFK
>Sapur.006G098600.1.v5.1
MALNDESAEKRKSARLAILELANMISVPMSLNAVVRLNVADAIWQGGSNTPLSASQILQRIASTGSDPENLQRILRMLTSYGVFEEHLTNESSPLDGSASERKYSLTEIGKTLVTDADGLSYAPYVLQHHQDALMKAWPLVHEAVLDPTTEAFVKANGEPAYDYYGKQEEMNELMLKAMSGVSVPFMKAMLDGYDGFKGVERLVDVGGSAGDCLRMILKKYPGVRHGINFDLPEVVAKAPNIPGVTHVGGDMFKSIPDGDAIFMKWILTTWTDNECKLIMENCYKALPEGGKLIACEPVLPKDSDDSHRTRALLEGDIFVMTIYRAKGKHRTGEEFMQLGQSVGFSHFRAFHVDYFYTVLEFQK
>Sapur.012G002900.1.v5.1
MGSTGDQTQMTPTQVSDEEAHLFAMQLASASVLPMILKTAIELDLLEIMAKAGPGAFLSSSEIASHLPTKNPDAPVMLDRILRLLASYSILTCSLKDLPDGKVERLYGVAPVCKFLTKNEDGVSVSPLCLMNQDKVLMESWYYLKDAILDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTITMKKILETYKGFEGLKSLVDVGGGTGAVVNTIVSKYPSIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSIPKADAVFMKWICHDWSDEHCLSFLKKCYDALPENGKVILVECILPVAPDTSLATKGVVHVDVIMLAHNPGGKERTEKEFEGLAKGAGFQGFEVMCCAFNTYVIEFRKKA
>Sapur.013G111700.1.v5.1
MTLSDLVSSLPIHPSKTQYVHRIMHILVHSGFFSQQNNDQEIYSLSQSGRLLVEDNPLSIRSLVLMLLDPVLTKPWDCLSTWFRNDEVAAFSVAHGKTIWEYTDQDPRLNNLFNAAMASDSILVSKVLVSKCKGIFEGVNFLVDVGGGIGTMAKAISEAFPHIHCTVFDLPHAVSDLQGSKNLEYVVGDMFEAVPSADTLLLKWILHDWSDEDCIKIIP
>Sapur.014G084100.1.v5.1
MGRKNQKQKNQKHRVSQSMPMAASVTEEEATQNDNQKQTVEEERESFTNAMTLVNASVLPLALKTVIDLGVLEVLATADPDVGLTAAEIAKRIPALNPEAPVMLERILRLLMSEGVVYCSHALFDEAPMKYRLGRVGKYFVRDDSGVSLAPLMALAHDKVYLETWSNLKDAILEGRTPFNRAHGTNLFEYSARDARFSQVYNTAMFNHTNLVFKKILESYSGFENLKQVVDVGGGIGVALSLIAFKYPFINAINFDLPHVIQHAPPFPGVKHVEGDMFKSVPKGDAIILKWILHDWDDEHCLTLLKNCYLSVPADGKIIVVEQILPTFAEITAVSRDKSQHDMISLTQTPGGKERMQNQLFNLAFSAGFKSVRLVSYVYHYWVMEFMK
>Sapur.014G084300.1.v5.1
MGSLKESHVGHVAEAKDENFGYAMQLALSSVLPMTLFSAIELGVFEIIAKAGPDAKLSASDVAAQLPTTNPDAPMMLDRILRLLASHDVLACSVDGLERLYSLAPVSKHYVRNEDGVSLGPFMALIQDNVFLKSWSQLKDSVLEGGVAFDRVHGVHSFEYTGLDPRFNQVLNTAMYNQTTIILENMLEAYSGFKNLKTLVEIGGGMGHTIKAIISKYPQIKGINFDLPHVIEHAPPCRGVEHVGGDMFGSVPKGDAIFLKWILHDWSDDRCLKLLKNCYKAIPDNGKVIVMESVLSITAKSSPAARAISQLDVLMMTQNPGGKERTEHEFMALATGAGFRGIRYEAFACNFWVMEFFK
>Sapur.15WG002100.1.v5.1
MGSTGEAQMSPAQALNEDANCALLLVSSSVLPMVLKTAVELDLLEIMAKAGPGASMSPSDIASHLPTKNPDAPSMLDRILRLLASYSILTCSLRDLPDGKVERLYGLASVCKFLTKNEDGGSLGPLCLMNQDKVLMESWYHLKDAILDGGVPFNKAYGMTAFEYHGKDPRINKVFNKGMSDHSTITMKSILETYRGFEGIASLVDVGGGTGAVISAIVSKYPSIKGINFDLPHVIADAPSFPGVENVGGDMFVSVPKADAVFMKWICHDWSDEHCFKLLKNCYDALPENGKVILVDCIIPVVPDTSLATKGVIHADVIMLAHNPGGKERTEKEFESLAKGCGFKGLEVMCCVFNTYVIEFRKQL
>Sapur.15ZG002100.1.v5.1
MGSTGEAQMSPAQALNEDANCALLLVSSSVLPMVLKTAVELDLLEIMAKAGPGASMSPSDIASHLPTKNPDAPSMLDRILRLLASYSILTCSLRDLPDGKVERLYGLASVCKFLTKNEDGGSLGPLCLMNQDKVLMESWYHLKDAILDGGVPFNKAYGMTAFEYHGKDPRINKVFNKGMSDHSTITMKSILETYRGFEGIASLVDVGGGTGAVISAIVSKYPSIKGINFDLPHVIADAPSFPGVENVGGDMFVSVPKADAVFMKWICHDWSDEHCFKLLKNCYDALPENGKVILVDCIIPVVPDTSLATKGVIHADVIMLAHNPGGKERTEKEFESLAKGCGFKGLEVMCCVFNTYVIEFRKQL
>Sapur.016G291000.1.v5.1
MATSSDEEDYHLQYALQLASASTLPMVFKAVIELGVLEIIEKAGPAAMLSASQIASQLPTQNNPDAHIVLDRILSLLASHSILTCSLATENQDSDQIQRLYGLAPVAKYFIRKEDGGSLSPYFRVIHDKVIMDLWYHLKDVVLQGGALFQKAYGMSSMEYVKKDPRFGEVFGGFVRGFNPLFMKRILDIYDGFEGLTSLVDVGGGDGSVLNMIVSKYPAIKGINFDLASVIENSPSYPGIEHVAGDVFLTIPEGEAIFMKWVSHFFSDDNFLKVLKNCYGALPDNGKLIVVEMVIPEIPGTSAADRSLMQNYLFVTSMNPKRNERTEKDFERLAKVAGFSHFRVACSACTFSVVEFVKNKATAGAVAIAAK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University