Enzyme
One of three specific glucosyltransferases in pea (Pisum sativum) that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside (see also EC 2.4.1.91, flavonol 3-O-glucosyltransferase and EC 2.4.1.240, flavonol-3-O-glycoside glucosyltransferase). TDP-glucose can replace UDP-glucose as the glucose donor but the reaction proceeds more slowly.
UGT79B30
Enzyme
UDP-glycosyltransferase 79B30
Enzyme name
EC:2.4.1.239
Entry
Transferases;Glycosyltransferases;Hexosyltransferases
Class
UDP-glucose:flavonol-3-O-beta-D-glucoside 2''-O-beta-D-glucosyltransferase
Sysname
UDP-glucose + a flavonol 3-O-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside [RN:R07339]
Reaction
UDP [CPD:C00015];flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside [CPD:C15581]
Product
Species information
Salix purpurea
Species name
Purple osier (Salix purpurea) is a species of eudicot in the family Salicaceae (willow family).
Species info
Gene sequence info
Salix_purpurea.UGT79B30.fa
Download
>Sapur.011G041800.1.v5.1
MSSNIAAHEGIKTLHIAMYPWFALGHLTAFVHFSNKLAERGHRISFFLPKNTQSKFEPFNLHPDLITFIPITVPHVHGLPTGTETTADVPFPLQPLLMTAMDLTEHAIEDHLRILNPHFIFFDFTHWLPELSRKLGIKSVHFCIISPATIGYLLSPERKLESLTAADLMQPPPSFPLSSIKLRAHEARGILAVTVQQYGRNISFHERHLYALSQCDAMAFKTCREMEGPYSDYIEDQFGKPVILAGPIVPEPPKSVLEEEIAKVLDTFKAGTLVFCAFGSECVLKKDQFQELVLGLELTGLPFFAVLKPPTGAETIESALPEKFEERVKGRGFVYTGWVQQQLILRHPSVGCFVTHCGSGSLSEGMVNTCQLVLLPNVGDQIINARVVGGDLKVGVEVEKGEEDGLFTRHGVCEAVKAVMDDDREVGKLVRANHAKWREFMLGEGLENSYVDGFVDKLHELLE
>Sapur.017G031400.1.v5.1
MPRLVRGLGIKSIYYYVVSSVTIDIGYIMSLARRLSGNKLTKASLMRPPLGFPDLPIKLKAHEAEVYIARRIMKFGGDVMFCDRIYMGMSQCDAIGFRTCREIEGPYADYVGNQFDKPVLFSGPVIPEPCTSALEEKWYKWLGEFKASSVIYCAFGSECTLEKDQFEELLLGLELTGMPFMAALKPPAGHESIELALPQGFQERICGRGIVHGGWVQQQLILEHPSVGCFITHCGSGSLSEALINKCQLVLLPHVGDHIFQARMMSLHLKMGVEVEKGEEDGLFTKETVYKAVRMLMEEESEFSREVKTNRAKLREFLSSKTLESSYIDSFNEQIQALLG
MSSNIAAHEGIKTLHIAMYPWFALGHLTAFVHFSNKLAERGHRISFFLPKNTQSKFEPFNLHPDLITFIPITVPHVHGLPTGTETTADVPFPLQPLLMTAMDLTEHAIEDHLRILNPHFIFFDFTHWLPELSRKLGIKSVHFCIISPATIGYLLSPERKLESLTAADLMQPPPSFPLSSIKLRAHEARGILAVTVQQYGRNISFHERHLYALSQCDAMAFKTCREMEGPYSDYIEDQFGKPVILAGPIVPEPPKSVLEEEIAKVLDTFKAGTLVFCAFGSECVLKKDQFQELVLGLELTGLPFFAVLKPPTGAETIESALPEKFEERVKGRGFVYTGWVQQQLILRHPSVGCFVTHCGSGSLSEGMVNTCQLVLLPNVGDQIINARVVGGDLKVGVEVEKGEEDGLFTRHGVCEAVKAVMDDDREVGKLVRANHAKWREFMLGEGLENSYVDGFVDKLHELLE
>Sapur.017G031400.1.v5.1
MPRLVRGLGIKSIYYYVVSSVTIDIGYIMSLARRLSGNKLTKASLMRPPLGFPDLPIKLKAHEAEVYIARRIMKFGGDVMFCDRIYMGMSQCDAIGFRTCREIEGPYADYVGNQFDKPVLFSGPVIPEPCTSALEEKWYKWLGEFKASSVIYCAFGSECTLEKDQFEELLLGLELTGMPFMAALKPPAGHESIELALPQGFQERICGRGIVHGGWVQQQLILEHPSVGCFITHCGSGSLSEALINKCQLVLLPHVGDHIFQARMMSLHLKMGVEVEKGEEDGLFTKETVYKAVRMLMEEESEFSREVKTNRAKLREFLSSKTLESSYIDSFNEQIQALLG
Sheng jun & Dong yang Teams, Yunnan agriculture University