Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Aquilegia coerulea
Species name
Rocky mountain columbine (Aquilegia coerulea) is a species of flowering plant in the family Ranunculaceae (buttercup family).
Species info
Gene sequence info
Aquilegia_coerulea.4CL.fa
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>Aqcoe1305s0001.1.p
MTEAGALSMCLAFAKEPHKIKSGSCGTVIRNAEMKIVDLKTGACLHQNQSGEICIRGSQIMKGYLNDPVATANTVDIDGWLHTGDVGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHPNISDAAVVPMKDDVAGEVPVAFVVRSKGSKISEEEIKQYISKQVVFYKRISKVFFQDTILKAPSGKILRKELRKKLDDSFSN*
>Aqcoe4G203600.1.p
MGVLEQNQDIIFRSKLPDIYIPNHLPLHSYCFENISEVCTRPCLINGTTGETHTYEEVELIARKVASGLNKIGIQQGQVMMILLPNSLEFVFAFLGASYRGVTVTTANPFYTSAEITKQAKASNARIIITLATHVNKLKNFAQEHDVKIMCIDSPPEGCLHFSELTQSDETELPAVKIKPEEDIVALPFSSGTTGLPKGVMLTHKGLITSIAQHVDGENPNLYIHKEDVSLCVLPLFHIFSLTSILLSGLRVGATILIMPKFELKQLMELVEKYKVTIAPFVPPIVLAIAKSPDLHKYDLSSIRTLISGAAPMGKELEDTLRAKLPTAKLGQGYGMTEAGALSMCLAFAKEPHKIKSGSCGTVIRNAEMKIVDLKTGACLHQNQSGEICIRGSQIMKGYLNDPVATANTVDIDGWLHTGDVGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHPNISDAAVVPMKDDVAGEVPVAFVVRSNGSKISEEEIKQYISRQVVFYKRISKVFFQDTILKAPSGKILRKELRKKLDDSFSN*
>Aqcoe7G241700.1.p
MGTLEKQMLVEEEEEHVFKSQFSAVPVPENMTLPEFVLHEAELYADKVAIVEPVTGKEFTYAQVTRDTRRFCKALRSLGLRKGKVVVVVLPNVAEYAIIALGIMAAGGVFSGANPQAHTSEIKKQVEASDAKLIVTIGSIYEKVKDLGVPVIVLGEERIDNTICWEDLLDASDHAGSGAMNETIKQDDLCALPFSSGTTGTSKGVMLTHKNLVANLCSTLFSVGPDIIGQVTTLGLMPFFHIYGIVGICCATLRNKGKVVVMGRFELRTFLNALITHEISFAPIVPPIMLALVKNPVVGEFDLSRLKLRAVMTAAAPLAPELLSAFEEKFPGVQVQEAYGLTEHSCITLTHGDPRKGHCTAKKSSVGFILPNLEIKFLDPDTGRSLPKNIPGEICVRSQCVMQGYYKNKEETDRTIDTKGWLHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLSHPSVEDAAVVPLPDEEAGEIPAACVVMNKNAKETEEEIMNFVASNVAAYKKVRVVHFVDTIPKSPSGKIMRRYLKEKLEKKKEVSSLSGAHNWTAT*
>Aqcoe7G325200.1.p
MGALEQSQDIIFRSKLPAIYIPNHLPLHSYCFENISEFSSRPCLINGTTGEIHTYENVELIARKVASGLNKIGIQQGQVIMILLPNSPEFVFAFLGASYRGAIVTTANPFYTPAEIAKQAKASNTRLIITLASYVDKLKELAQDDDVKVMCIDSPPEGCLHFSELIQSDETELPDVKIRPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHKEDVVVCVLPMFHIYSLNSVLLCGLRVGAAILILPKFEIKQLMELVEKYKVTIAPFVPPIVLAIVKSPDLHKYDLSSIRTLMSGAAPMGKELEDTLRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPHEIKSGSCGTVVRNAEMKIVDPETGASLPRNQPGEICIRGSQIMKGYLNDPEATANTIDIDGWLHTGDVGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPNISDAAVVPMKDDAAGEVPVAFVVKSNGSKISEEDIKQYISKQVVFYKRISKVFFQETIPKAPSGKILRKELRKKLADGFPN*
>Aqcoe0099s0002.1.p
MISIATPETNTTPQTQVSNSDIEINSSETFVFRSKLPDIPISYELPLHDYCFEKLSEVSDKPCLISGSTGKIYTYAETHLLCQRTSVGLSTLGVKKGDVIMIILQNCPEFVFSFIGASMLGATTTTANPFYTPAEIFKQMATSGSKLVITQSQYVDKLRCVTENSAKIGKDFTVVTIDDKQENCIHFSEISGGDEKKVQPVAFDPNDAVALPFSSGTTGLPKGVILTHKSLISSIAQQVDGENPNLYLKSDDIVLCVLPLFHIFSLHSVLLCSLRAGVGVLIMPKFEIGALLELIQTYRVSVAPVVPPLVLALAKNPALEKFDLSSIRIVLSGAAPLGKELEEALKCRLPQAIFGQGYGMTEVGPVLSMCSGFAKQPFPTKSGSCGTVVRNAELKILDPETGLSLRRNQRGEICIRGPQIMKGYLNDAVATASTIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKYKGFQVPPAELESLLISHPSIADAAVVPQMDTLAGEVPVAFVVRSNDLDLTEEAVKEFIAKQVVFYKRLHKVFFIHAIPKSPAGKILRKDLRAKLVSSS*
>Aqcoe1G133100.1.p
MEKSGYGRDGIYRSLRPPLILPRDLNISMVSFLFRNSNSYSDKLALIDADSEETLTFGQFKSTVAKVSHGLLQLGIKKGDVVLIFAPNSIQFPISFLGIVAIGAIATTVNPLYTVTEISKQIKDSKPKLVVTVPQLWEKLKDFGLPAVILGSQDSSMIVSKSKIVTFSELVKLAGGGVSDLPLNTVKQTDTAALLYSSGTTGVSKGVILTHRNFIASSLQSSVDLEFYGERNNIFLCFLPMFHIFGLAIITLGQLQRGSTIISVGRFDIEMVLKSVERYKVTNLFVVPPVVLALAKQNLVNKYDLSSLRQLGSGAAPLGKDLMEECAKNLPQATVLQGYGLTESCGIVSLENAYEGTRHSGSAGTLVPGVESKIVSVDTMKALPPTELGEIWIRGPNMMQGYFNNPEATKLTIDKQGWVHTGDLGYFDERGQLFVVDRIKELIKYKGYQVAPAELEGLLLTHPEINDAVVIPFPDAEAGEVPIAYVVRSPNSSLTEEAVKNFIAKQVAPFKRLRRVTFINTVPKSASGKILRRELIDKVRSKM*
MTEAGALSMCLAFAKEPHKIKSGSCGTVIRNAEMKIVDLKTGACLHQNQSGEICIRGSQIMKGYLNDPVATANTVDIDGWLHTGDVGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHPNISDAAVVPMKDDVAGEVPVAFVVRSKGSKISEEEIKQYISKQVVFYKRISKVFFQDTILKAPSGKILRKELRKKLDDSFSN*
>Aqcoe4G203600.1.p
MGVLEQNQDIIFRSKLPDIYIPNHLPLHSYCFENISEVCTRPCLINGTTGETHTYEEVELIARKVASGLNKIGIQQGQVMMILLPNSLEFVFAFLGASYRGVTVTTANPFYTSAEITKQAKASNARIIITLATHVNKLKNFAQEHDVKIMCIDSPPEGCLHFSELTQSDETELPAVKIKPEEDIVALPFSSGTTGLPKGVMLTHKGLITSIAQHVDGENPNLYIHKEDVSLCVLPLFHIFSLTSILLSGLRVGATILIMPKFELKQLMELVEKYKVTIAPFVPPIVLAIAKSPDLHKYDLSSIRTLISGAAPMGKELEDTLRAKLPTAKLGQGYGMTEAGALSMCLAFAKEPHKIKSGSCGTVIRNAEMKIVDLKTGACLHQNQSGEICIRGSQIMKGYLNDPVATANTVDIDGWLHTGDVGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHPNISDAAVVPMKDDVAGEVPVAFVVRSNGSKISEEEIKQYISRQVVFYKRISKVFFQDTILKAPSGKILRKELRKKLDDSFSN*
>Aqcoe7G241700.1.p
MGTLEKQMLVEEEEEHVFKSQFSAVPVPENMTLPEFVLHEAELYADKVAIVEPVTGKEFTYAQVTRDTRRFCKALRSLGLRKGKVVVVVLPNVAEYAIIALGIMAAGGVFSGANPQAHTSEIKKQVEASDAKLIVTIGSIYEKVKDLGVPVIVLGEERIDNTICWEDLLDASDHAGSGAMNETIKQDDLCALPFSSGTTGTSKGVMLTHKNLVANLCSTLFSVGPDIIGQVTTLGLMPFFHIYGIVGICCATLRNKGKVVVMGRFELRTFLNALITHEISFAPIVPPIMLALVKNPVVGEFDLSRLKLRAVMTAAAPLAPELLSAFEEKFPGVQVQEAYGLTEHSCITLTHGDPRKGHCTAKKSSVGFILPNLEIKFLDPDTGRSLPKNIPGEICVRSQCVMQGYYKNKEETDRTIDTKGWLHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLSHPSVEDAAVVPLPDEEAGEIPAACVVMNKNAKETEEEIMNFVASNVAAYKKVRVVHFVDTIPKSPSGKIMRRYLKEKLEKKKEVSSLSGAHNWTAT*
>Aqcoe7G325200.1.p
MGALEQSQDIIFRSKLPAIYIPNHLPLHSYCFENISEFSSRPCLINGTTGEIHTYENVELIARKVASGLNKIGIQQGQVIMILLPNSPEFVFAFLGASYRGAIVTTANPFYTPAEIAKQAKASNTRLIITLASYVDKLKELAQDDDVKVMCIDSPPEGCLHFSELIQSDETELPDVKIRPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHKEDVVVCVLPMFHIYSLNSVLLCGLRVGAAILILPKFEIKQLMELVEKYKVTIAPFVPPIVLAIVKSPDLHKYDLSSIRTLMSGAAPMGKELEDTLRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPHEIKSGSCGTVVRNAEMKIVDPETGASLPRNQPGEICIRGSQIMKGYLNDPEATANTIDIDGWLHTGDVGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPNISDAAVVPMKDDAAGEVPVAFVVKSNGSKISEEDIKQYISKQVVFYKRISKVFFQETIPKAPSGKILRKELRKKLADGFPN*
>Aqcoe0099s0002.1.p
MISIATPETNTTPQTQVSNSDIEINSSETFVFRSKLPDIPISYELPLHDYCFEKLSEVSDKPCLISGSTGKIYTYAETHLLCQRTSVGLSTLGVKKGDVIMIILQNCPEFVFSFIGASMLGATTTTANPFYTPAEIFKQMATSGSKLVITQSQYVDKLRCVTENSAKIGKDFTVVTIDDKQENCIHFSEISGGDEKKVQPVAFDPNDAVALPFSSGTTGLPKGVILTHKSLISSIAQQVDGENPNLYLKSDDIVLCVLPLFHIFSLHSVLLCSLRAGVGVLIMPKFEIGALLELIQTYRVSVAPVVPPLVLALAKNPALEKFDLSSIRIVLSGAAPLGKELEEALKCRLPQAIFGQGYGMTEVGPVLSMCSGFAKQPFPTKSGSCGTVVRNAELKILDPETGLSLRRNQRGEICIRGPQIMKGYLNDAVATASTIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKYKGFQVPPAELESLLISHPSIADAAVVPQMDTLAGEVPVAFVVRSNDLDLTEEAVKEFIAKQVVFYKRLHKVFFIHAIPKSPAGKILRKDLRAKLVSSS*
>Aqcoe1G133100.1.p
MEKSGYGRDGIYRSLRPPLILPRDLNISMVSFLFRNSNSYSDKLALIDADSEETLTFGQFKSTVAKVSHGLLQLGIKKGDVVLIFAPNSIQFPISFLGIVAIGAIATTVNPLYTVTEISKQIKDSKPKLVVTVPQLWEKLKDFGLPAVILGSQDSSMIVSKSKIVTFSELVKLAGGGVSDLPLNTVKQTDTAALLYSSGTTGVSKGVILTHRNFIASSLQSSVDLEFYGERNNIFLCFLPMFHIFGLAIITLGQLQRGSTIISVGRFDIEMVLKSVERYKVTNLFVVPPVVLALAKQNLVNKYDLSSLRQLGSGAAPLGKDLMEECAKNLPQATVLQGYGLTESCGIVSLENAYEGTRHSGSAGTLVPGVESKIVSVDTMKALPPTELGEIWIRGPNMMQGYFNNPEATKLTIDKQGWVHTGDLGYFDERGQLFVVDRIKELIKYKGYQVAPAELEGLLLTHPEINDAVVIPFPDAEAGEVPIAYVVRSPNSSLTEEAVKNFIAKQVAPFKRLRRVTFINTVPKSASGKILRRELIDKVRSKM*
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University