Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Salix purpurea
Species name
Purple osier (Salix purpurea) is a species of eudicot in the family Salicaceae (willow family).
Species info
Gene sequence info
Salix_purpurea.ROMT.fa
Download
>Sapur.013G111600.1.v5.1
MELINEESASELLQAQTHVWNHIFNFINSMTLKCAVQLGIPDVIHKHGKPMSLSELVSSLPVHPSKTQYVHRIMRILVHSGFFSQQNNNQEIYSLSQSGRLLLEDNPLSIRSLLLMDLGPILTKPWDCLSTWFQNDEVTAFSVAHGRTFWEYAGQDPRLNNLFNAAMASDSVLVSKVIVSKCKGIFEGLNSLVDVGGGVGTMAKAISEAFPHIHCIVFDLPHVISDLQDSKNLEYAAGDMLEAVPPADALLLKWILHNWSDEDCIKILQQCKQAIMRNGKQKVGKVIIIDIVRENNNRDEVSTETQLFFDLLMMVNLNGMERNEKEWAKLFFYAGFLNYKIHPVLGTRSLIELHL
>Sapur.013G111500.1.v5.1
MELINEDSASELLQAQTHVWNHVFNFINSMTLKCAVQLGIPDAIHKHGKPMTLSELVSALPIHPSKTQYVHRLMRILVHSGVFSQQNLNGINNNQEVYSLSQFAHLLLEDSPLSMRPLLLILLDPVFTKPWDCLSTWFRNDEVAAFSVAHGKTIWEYTGQDPRLNNLFNEAMASDSILVSKVIVSKCKGIFEGVNSLVDVGGGTGTMAKAISEAFPHIDCTVFDLPCVVSDLQGSKNLKYVAGDMFEIVPPADAVLLKWILHDWSDEDCIKILQQCKQAITRNGKQKVGKVIVIDMVRENKNRDEAVSTETQLFFDLEMMVTLNGMERNEKEWAKLFFDAGFLNYKIHPVLGTRALIELHP
>Sapur.013G111800.1.v5.1
MELINEESASELLQAQTHVWNHIFNFINSMTLKCAVQLGIPDVIHKHGKPMTLSELVSALPIHPSKTQYVHRLMRVLVHSGFFSQQNLNGVHSQEAYFLTPSTHLLLRDNPLSMRPLLLLLLDPVLTEPHDCLSTWLQNDEVTAFSVAHGRTMWEYASQDPRLGNLFNEAMASDSILVSKLVVSRCKGMFEGVNSLVDVGGGSGAMAKGIAEAFPHVDCTVFDLPRVVSDLQGSKNLKYVGGDMFEGVPPADMILLKWTLHDWRDGDCIKILKQCKTAIMGKGQRKAGKVMIIDIVRGNQNDDESNETQQLWDLEMMVTVAGLERNEMEWAKLFLDAGFISYKIHNVLGTRALIELHP
>Sapur.013G111700.1.v5.1
MTLSDLVSSLPIHPSKTQYVHRIMHILVHSGFFSQQNNDQEIYSLSQSGRLLVEDNPLSIRSLVLMLLDPVLTKPWDCLSTWFRNDEVAAFSVAHGKTIWEYTDQDPRLNNLFNAAMASDSILVSKVLVSKCKGIFEGVNFLVDVGGGIGTMAKAISEAFPHIHCTVFDLPHAVSDLQGSKNLEYVVGDMFEAVPSADTLLLKWILHDWSDEDCIKIIP
>Sapur.019G089000.1.v5.1
MSLAEGNLHGAELLQAQTHVWNHIFNFINSMSLKCAVELGIPDIILNHGKPMTLSELVAVLPIHPTKAPFVYRLMRILVHSGFFAQQNGSGNTVGEGYVLTNASQLLVKDNPFSVTPLLLAELDPILTQPWHHVSAWFQNDDPSPFHTAHERTFWEYASQEPKLNQFFNEAMASDARLVSSVMINECKGVFEGLNSLVDVGGGTGTVAKAIAKEFQHLDCTVFDLPHVVAELEGSGNLKYLGGDMFEGIPQADAILLKWILHDWNDEECVKILKQCKEAIKGREGGKVIVIDMVVENNKRDAGSTETQLFFDMLMMVMVTGKQRNKNEWSKLFSDAGFSNFKINPVLGLRSLIEVYP
>Sapur.019G089100.1.v5.1
MLEKSMELIDEEGANELLQAQTRVWNHALAFINSMALKCAVQLDIPDAIQNHGKPITLSELVSSLPIHPSKSQHLYRLMRILVHSGIFSLKKEGYSLTPTSRLLLKGNPMSARPLLLFWLDPVPMEPLRCLSTWFRNEDPTAFDTAHGKCIWELAGENARLNNLFNEAMASDGALACGVVMKKCEGVFEGLKSLVDVGGGTGAMAKCIAETFPTMNCTVFDLPHVVSGLPGSKNLKFVGGDMFESVPSADAILLKWILHDWSDEECVKILKQCKEAIGRNGNIGGKVIIMEMILGNQNWDENSIETQLFFDLYIMTCVTGRERSEDEWAKLFIAAGFSNYKINPVLGVRALIEVYP
>Sapur.019G088200.1.v5.1
MHSAEGNLHGAELLQAQAHVWNHIFNFINSMSLKCSVELGIPDLIHNHGKPMTLAQLVAVLPIHPTKTHFVYRLMRILVHSGFFVQQNGSGDTEGEGYVLTNASQLLLKDNPFSVTPFLLAYLDPILTQPWHHVSAWFQNDVPSPFHTAHERTLWEYASHEPKLNQFFNEAMASDARLVSSVMINECKGVFDGLNSLVDVAGGTGTLAKALAREFQHLDCTVFDLPHVVADLEGSGNLKYLGGDMFEEIPQADAILLKWILHDWNDEECVKILRQCKEAIKGREGGKVIIIDIVVENNKVEARSTETQLYFDMLMMILFTGKERNKNEWSQLFSDAGFTNFKITPVLGLRSLIEVYP
>Sapur.019G088500.1.v5.1
MHLAEGNLHGTEILLAQAHVWNHIFNFINSMSLKCAVELGIPDIILNHGKPMTLSELVAVLPIHPTKAPFVYRLMRILVQSGFFAQQNASGNTKGEGYVLTNASQFLVKDNPLSVAPFLLAMLDPILTQQWHHVSVWFQNDVPSPFDTAHERTLWEYASHETKLNQFFNEDMASDARLVSSVMINECKGVFEGLNSLVDVGGGTGTVAKAIAKEFQHLDCTVFDLPHVVADLEGSGNLKYLGGDMFEEIPQWILHDWNDEECGREGGKVIIIDMVVENNKVEAGSTETQFFFDMLMMILVTGKERKKSEWSKLFSDAGFSNFKINPVPGLRSLIEVYP
>Sapur.019G088800.1.v5.1
MELINEEGANELLQAQTSVWNHALEFINSMALKCAIELEIPDAIQSHGKPITLSELVSSLPIHPSKGEYLYRLMRILVHSGIFSLKKVGDDPQQEGYSLTPTSCFLLKGNPLSVRPLLLFWLDPVPMEALQCLSTWFQNEDLTAFNTAHGKCAWELTGKNARLNNLFNEAMASDGALTSGVVMKKCKGVFEGLKSLVDVGGGTGAMAKCIAETFPTMNCIVFDLPHVVSGLQGSDKLKFVEGDMFESVPSADAILLKWILHDWSDEECVKILKQCKEALGRNDKIGGKVIIIDMILGNQTWDEKSTETQLFFDLFIMTCVSGRERSEKELANLFFAAGFSNYKINPVLGVRGLIEVYP
>Sapur.019G088900.1.v5.1
MSLAEGNLHGDELLQAQAHVWNHIFNFINSMSLKCAVELGIPDLILNHGKPMTLSELVAVLPIHPAKAPFVYRLMRILVHSGFFAQQNGSGSTEGEGYVLTNASQLLVKDNPFSVAQFLLAMLDPILTQPWHHVSAWFQNDVPSPFHTAHERTFWEYASQEPKLNQFFNEAMASDARLVSSVMKNECKGVFEGLNSLVDVGGGTGTVAKAIAKEFQHLDCTVFDLPHVVADLEGSGNLKYLGGDMFEEIPQADAILLKWILHD
>Sapur.019G088400.1.v5.1
MKMSLAEGNLHGAELLQAQAHVWHHILNFINSMSLKCAVELGIPDLIHNHGKPMTLSELVAVLPIHPTKAPFVYRLMRILVHSGFFAQQNASGNTEGEGYVLTNASQLLVKDNPFSVAQFVLGNLDPTTIQPWYHVSAWFQNNVPSPFQTAHERTFWEYSHEPKLNQLFNDAMASDARLVSIVLTNECKGVFEGINSLVDVGGGTGTVAKAIAKEFQHLDCTVFDLPYVVADLEGSGNLKYLGGDMFEEIPQADAILLKWVLHDWNDEECVKILRQCKEAIKGREGGKVIIIDIVVENNEVEAGSTETQLCFDMLMMIVCTGKERNKNEWSKLFSDAGFSNFKISPVLGLRSLIEVYP
>Sapur.019G088700.1.v5.1
MELINEEGANELLQAQTSVWNHALEFINSMALKCAIELEIPDAIQSHGKPITLSELVSSLPIHPSKGEYLYRLMRILVHSGIFSLKKVGDDPQQEGYSLTPTSCFLLKGNPLSVRPLLLFWLDPVPMEALQCLSTWFQNEDLTAFNTAHGKCAWELTGKNARLNNLLNEAMASDGALTSGVVMKKCKGVFEGLKSLVDVGGGTGAMAKCIAETFPTMNCIVFDLPHVVSGLQGSDNLKFVEGDMFESVPSADAILLKWILHDWSDEECVKILKQCKEAIGRNDKIGGKVIIIDMILGNQNWDEKSTETQLFFDLFIMTCVSGRERSEKELANLFFAAGFSNYKINPVLGVRGLIEVYP
MELINEESASELLQAQTHVWNHIFNFINSMTLKCAVQLGIPDVIHKHGKPMSLSELVSSLPVHPSKTQYVHRIMRILVHSGFFSQQNNNQEIYSLSQSGRLLLEDNPLSIRSLLLMDLGPILTKPWDCLSTWFQNDEVTAFSVAHGRTFWEYAGQDPRLNNLFNAAMASDSVLVSKVIVSKCKGIFEGLNSLVDVGGGVGTMAKAISEAFPHIHCIVFDLPHVISDLQDSKNLEYAAGDMLEAVPPADALLLKWILHNWSDEDCIKILQQCKQAIMRNGKQKVGKVIIIDIVRENNNRDEVSTETQLFFDLLMMVNLNGMERNEKEWAKLFFYAGFLNYKIHPVLGTRSLIELHL
>Sapur.013G111500.1.v5.1
MELINEDSASELLQAQTHVWNHVFNFINSMTLKCAVQLGIPDAIHKHGKPMTLSELVSALPIHPSKTQYVHRLMRILVHSGVFSQQNLNGINNNQEVYSLSQFAHLLLEDSPLSMRPLLLILLDPVFTKPWDCLSTWFRNDEVAAFSVAHGKTIWEYTGQDPRLNNLFNEAMASDSILVSKVIVSKCKGIFEGVNSLVDVGGGTGTMAKAISEAFPHIDCTVFDLPCVVSDLQGSKNLKYVAGDMFEIVPPADAVLLKWILHDWSDEDCIKILQQCKQAITRNGKQKVGKVIVIDMVRENKNRDEAVSTETQLFFDLEMMVTLNGMERNEKEWAKLFFDAGFLNYKIHPVLGTRALIELHP
>Sapur.013G111800.1.v5.1
MELINEESASELLQAQTHVWNHIFNFINSMTLKCAVQLGIPDVIHKHGKPMTLSELVSALPIHPSKTQYVHRLMRVLVHSGFFSQQNLNGVHSQEAYFLTPSTHLLLRDNPLSMRPLLLLLLDPVLTEPHDCLSTWLQNDEVTAFSVAHGRTMWEYASQDPRLGNLFNEAMASDSILVSKLVVSRCKGMFEGVNSLVDVGGGSGAMAKGIAEAFPHVDCTVFDLPRVVSDLQGSKNLKYVGGDMFEGVPPADMILLKWTLHDWRDGDCIKILKQCKTAIMGKGQRKAGKVMIIDIVRGNQNDDESNETQQLWDLEMMVTVAGLERNEMEWAKLFLDAGFISYKIHNVLGTRALIELHP
>Sapur.013G111700.1.v5.1
MTLSDLVSSLPIHPSKTQYVHRIMHILVHSGFFSQQNNDQEIYSLSQSGRLLVEDNPLSIRSLVLMLLDPVLTKPWDCLSTWFRNDEVAAFSVAHGKTIWEYTDQDPRLNNLFNAAMASDSILVSKVLVSKCKGIFEGVNFLVDVGGGIGTMAKAISEAFPHIHCTVFDLPHAVSDLQGSKNLEYVVGDMFEAVPSADTLLLKWILHDWSDEDCIKIIP
>Sapur.019G089000.1.v5.1
MSLAEGNLHGAELLQAQTHVWNHIFNFINSMSLKCAVELGIPDIILNHGKPMTLSELVAVLPIHPTKAPFVYRLMRILVHSGFFAQQNGSGNTVGEGYVLTNASQLLVKDNPFSVTPLLLAELDPILTQPWHHVSAWFQNDDPSPFHTAHERTFWEYASQEPKLNQFFNEAMASDARLVSSVMINECKGVFEGLNSLVDVGGGTGTVAKAIAKEFQHLDCTVFDLPHVVAELEGSGNLKYLGGDMFEGIPQADAILLKWILHDWNDEECVKILKQCKEAIKGREGGKVIVIDMVVENNKRDAGSTETQLFFDMLMMVMVTGKQRNKNEWSKLFSDAGFSNFKINPVLGLRSLIEVYP
>Sapur.019G089100.1.v5.1
MLEKSMELIDEEGANELLQAQTRVWNHALAFINSMALKCAVQLDIPDAIQNHGKPITLSELVSSLPIHPSKSQHLYRLMRILVHSGIFSLKKEGYSLTPTSRLLLKGNPMSARPLLLFWLDPVPMEPLRCLSTWFRNEDPTAFDTAHGKCIWELAGENARLNNLFNEAMASDGALACGVVMKKCEGVFEGLKSLVDVGGGTGAMAKCIAETFPTMNCTVFDLPHVVSGLPGSKNLKFVGGDMFESVPSADAILLKWILHDWSDEECVKILKQCKEAIGRNGNIGGKVIIMEMILGNQNWDENSIETQLFFDLYIMTCVTGRERSEDEWAKLFIAAGFSNYKINPVLGVRALIEVYP
>Sapur.019G088200.1.v5.1
MHSAEGNLHGAELLQAQAHVWNHIFNFINSMSLKCSVELGIPDLIHNHGKPMTLAQLVAVLPIHPTKTHFVYRLMRILVHSGFFVQQNGSGDTEGEGYVLTNASQLLLKDNPFSVTPFLLAYLDPILTQPWHHVSAWFQNDVPSPFHTAHERTLWEYASHEPKLNQFFNEAMASDARLVSSVMINECKGVFDGLNSLVDVAGGTGTLAKALAREFQHLDCTVFDLPHVVADLEGSGNLKYLGGDMFEEIPQADAILLKWILHDWNDEECVKILRQCKEAIKGREGGKVIIIDIVVENNKVEARSTETQLYFDMLMMILFTGKERNKNEWSQLFSDAGFTNFKITPVLGLRSLIEVYP
>Sapur.019G088500.1.v5.1
MHLAEGNLHGTEILLAQAHVWNHIFNFINSMSLKCAVELGIPDIILNHGKPMTLSELVAVLPIHPTKAPFVYRLMRILVQSGFFAQQNASGNTKGEGYVLTNASQFLVKDNPLSVAPFLLAMLDPILTQQWHHVSVWFQNDVPSPFDTAHERTLWEYASHETKLNQFFNEDMASDARLVSSVMINECKGVFEGLNSLVDVGGGTGTVAKAIAKEFQHLDCTVFDLPHVVADLEGSGNLKYLGGDMFEEIPQWILHDWNDEECGREGGKVIIIDMVVENNKVEAGSTETQFFFDMLMMILVTGKERKKSEWSKLFSDAGFSNFKINPVPGLRSLIEVYP
>Sapur.019G088800.1.v5.1
MELINEEGANELLQAQTSVWNHALEFINSMALKCAIELEIPDAIQSHGKPITLSELVSSLPIHPSKGEYLYRLMRILVHSGIFSLKKVGDDPQQEGYSLTPTSCFLLKGNPLSVRPLLLFWLDPVPMEALQCLSTWFQNEDLTAFNTAHGKCAWELTGKNARLNNLFNEAMASDGALTSGVVMKKCKGVFEGLKSLVDVGGGTGAMAKCIAETFPTMNCIVFDLPHVVSGLQGSDKLKFVEGDMFESVPSADAILLKWILHDWSDEECVKILKQCKEALGRNDKIGGKVIIIDMILGNQTWDEKSTETQLFFDLFIMTCVSGRERSEKELANLFFAAGFSNYKINPVLGVRGLIEVYP
>Sapur.019G088900.1.v5.1
MSLAEGNLHGDELLQAQAHVWNHIFNFINSMSLKCAVELGIPDLILNHGKPMTLSELVAVLPIHPAKAPFVYRLMRILVHSGFFAQQNGSGSTEGEGYVLTNASQLLVKDNPFSVAQFLLAMLDPILTQPWHHVSAWFQNDVPSPFHTAHERTFWEYASQEPKLNQFFNEAMASDARLVSSVMKNECKGVFEGLNSLVDVGGGTGTVAKAIAKEFQHLDCTVFDLPHVVADLEGSGNLKYLGGDMFEEIPQADAILLKWILHD
>Sapur.019G088400.1.v5.1
MKMSLAEGNLHGAELLQAQAHVWHHILNFINSMSLKCAVELGIPDLIHNHGKPMTLSELVAVLPIHPTKAPFVYRLMRILVHSGFFAQQNASGNTEGEGYVLTNASQLLVKDNPFSVAQFVLGNLDPTTIQPWYHVSAWFQNNVPSPFQTAHERTFWEYSHEPKLNQLFNDAMASDARLVSIVLTNECKGVFEGINSLVDVGGGTGTVAKAIAKEFQHLDCTVFDLPYVVADLEGSGNLKYLGGDMFEEIPQADAILLKWVLHDWNDEECVKILRQCKEAIKGREGGKVIIIDIVVENNEVEAGSTETQLCFDMLMMIVCTGKERNKNEWSKLFSDAGFSNFKISPVLGLRSLIEVYP
>Sapur.019G088700.1.v5.1
MELINEEGANELLQAQTSVWNHALEFINSMALKCAIELEIPDAIQSHGKPITLSELVSSLPIHPSKGEYLYRLMRILVHSGIFSLKKVGDDPQQEGYSLTPTSCFLLKGNPLSVRPLLLFWLDPVPMEALQCLSTWFQNEDLTAFNTAHGKCAWELTGKNARLNNLLNEAMASDGALTSGVVMKKCKGVFEGLKSLVDVGGGTGAMAKCIAETFPTMNCIVFDLPHVVSGLQGSDNLKFVEGDMFESVPSADAILLKWILHDWSDEECVKILKQCKEAIGRNDKIGGKVIIIDMILGNQNWDEKSTETQLFFDLFIMTCVSGRERSEKELANLFFAAGFSNYKINPVLGVRGLIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University