Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Salix purpurea
Species name
Purple osier (Salix purpurea) is a species of eudicot in the family Salicaceae (willow family).
Species info
Gene sequence info
Salix_purpurea.4CL.fa
Download
>Sapur.001G045400.1.v5.1
MTQQTATNPIPCQFHTSKFHLSPNYSLIPCTRHDHRKFLTISPSYKTQLNFASLLPCLISTMGTPIPCSAQEEHIFQSRHPAVPVPDITLPDFVLQDAELYADKVAFVEAATGKTYTYAEVVRDTRRFAKALRSLGLRKGHVVVVALPNVAEYGIVALGIMTAGGVFSGANPTAHESELKKQAEAADAKLIVTDDLNYGKVKGLGLPVIVLGEESISTAVNWNELLDAADRAGDKLSYEEVLQSDLCALPFSSGTTGMSKGVMLTHRNLVANLCSSLFGVGPEMVGQVATLGLIPFFHIYGITGICCATLRNKGKVVVMGRFELRTFLNALITQEVNFAPIVPPIILALVKNPIVEEFDLSKLKLKAIMTAAAPLAPELLTAFENKFPGVQVQEAYGLTEHSCITLTHGDPSKGHGIAKKNSVGFLLPNLEIKFINPENGQSLPKNTPGEICVRSQCVMQGYFNNKEETARTIDADGWLHTGDIGYIDNDGDIFIVDRIKELIKYKGFQVAPAELEAILLSHPSVEDAAVVPLPDEEAGEIPAACVVMSKGAEESEEDIMEFVASNVARYKKVRVVQFVDSIPKSPSGKIMRRLLKEKMGSGSKASHFHK
>Sapur.001G029400.1.v5.1
MDTMMNPQEEFIFRSKLPDIYIPKNLPLHTYVFENLSKHSSKPCLINGANGDVYTYADVELTARRVASGLNKIGIQQGDVIMLFLPSSPEFVLAFLGASHRGAITTAANPFSTPAELAKHAKASKAKLLITQACYYEKVKDFSQENGVKIMCVDSAPDGCLHFSDLTQADENEMPQVDLHPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGENPNLYFHSEDVILCVLPMFHIYALNSIMLCGLRVGASILILPKFEIGSLLGLIEKYRVSIAPVVPPVMLAIAKSADLDKHDLSSVRMLKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFAKEPFDIKPGACGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPEATSRTIDKQGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLSHPEISDAAVVGMKDEDAGEVPVAFAVKSEKSQVTEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKNLREKLAGM
>Sapur.003G142800.1.v5.1
MDTMRKQKEEFIFRSKLPDIDIPKDLPLHSYVFENFSKYPSKPCLINGANGDIYTYADVELTARRVASGLNKVGIHQGDVIMLILPSSPEFVLAFLGASHRGAITTAANPFSTPAELAKQARASKSKLLITQACYYDKVKDYAQENDVKVVCVDSAPDECLHFSELTQADENDMPRAEFSPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSGDVILCVLPMFHIYALNSIMLCGLRAGAAILIMPKFEVGYLLGLIEKYKVSIAPVVPPVMLAIARSPDLDKHDLSSLRMLKSGGSPLGKELEDSVRAKFPQARLGQGYGMTEAGPVLAMCLAFAKQPFDIKPGACGTVVRNAEMKIVDPETGASLTRNQPGEICIRGDQIMKGYLNDPEATARTIDNEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLQAHPWISDAAVVGMKDEDAGEIPVAFVIKSENSQVTEEEIMQYISKQVIFYKRIKRVFFVEAIPKAPSGKILRKNLKERLAGGLQK
>Sapur.004G082800.1.v5.1
MEKSGYGRDGIYRSLRPKLVLPKDPNLSMVSFLFRNSNSYPHKPALIDADLSITLSYSELKSVVIKVAHGLLNLGISKNDVVLIFAPNSYQFPICFLAIISTGAVATTANPLYTTSELSKQIKDCNPKLIITVPELWDKVKGFNVPAVFLGPKRVPLPLESGSRIRSFDSLVELGGSSSQFPSSNVKQSDISTLLYSSGTTGVSKGVVLTHGNFIAASLMVTMDQVMAGEMHNVFLCFLPMFHVFGLAVVTYSQLQMGNAVVSMGKFEFEMVLKTIEKYRVTHMWVVPPVVLALAKQNLVKKYDLSSLRHIGSGAAPLGKDLMKECAKNLPGTIIAQGYGMTETSGIVSMEDSRLGTRHSGSAGMLAAGIEAQIVGVETLKPLPPTQLGEIWVRGLNMMKGYFNNPQATKDTIDGKGWVHTGDIGYFDEDGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPYPDAETGEVPVAYVVRSPNSALTEVDVKKFIADQVAPFKRLRKVTFVNSVPKSASGKILRRVLIEQVRSKI
>Sapur.006G138700.1.v5.1
MEAENDQAQEFIFRSKLPDIYIPSHLPLHTYCFQNLSHFKDNPCLINGSTGEIHTYADVELRSRKVASGLYKLGIKQGDVILLLLQNSPEFVFAFLGASYIGAVSTTANPFYTPAEVAKQATASKAKLIVTQAVYAEKVEQFAKENVHVRIVTIDSPPENYLHFSELTNSDENDIPAVKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCMLPLFHIYSLNSVLLCGLRVGAAILLMQKFEIITLMELVQKYKVTIAPFVPPVVLAVAKCSVVDKYDLSSIRTVMSGAAPMGKELEDAVRAKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPDTGKSLPRNQAGEICIRGCQIMKGYLNDPEATERTIDKYGWLHTGDVGYIDEDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDGAVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVVFYKRISRVFFTEEIPRAPSGKILRKDLKSRLVTGNLPY
>Sapur.017G093300.1.v5.1
MEKSGYGRDGIYRSLRPTLVLPKDSNLSLVSFLFRNSDSYPHKPALIDADLSITLSFSELKSIVIKVAHGLLNLGISKSDVVLIFAPNSYQFPICFLAITSIGAVATTANPLYTTSELSKQIKDSNPKLVITVPELWGKVKGFNLPAVFLGTKEVSLPLESGSRIRSFHSLVESGGSNSDFPASDVKQSDISTLLYSSGTTGVSKGVILTHGNFIAASLMVTMDQVTTGEMHNVFLCFLPMFHVFGLAVITYSQLQMGNAVAIMGKFEFEMVLKTIEKYRVTHMWVVPPVILALSKQSLVKKYDLSSLRNIGSGAAPLGKDLMKECAKNLPDTTITQGYGMTETSGVISVEDPRICARHSGSAGILTAGIEAQIVSVEAAVPLPPNQLGEIWVRGPNMMRGYLNNPQATKDTIDKKGWVHTGDLGYFDDDGLLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPYPDAEAGEVPVAYVVRSPNSTLSGEDIQKFIADQVAPFKRLRKVTFINTVPKSASGKILRRELVQKVKSKM
>Sapur.019G055800.1.v5.1
MMSVATVEPTKPEIPPPQSQNAPSSDETDHVFRSKLPDIPISNHLPLHTYCFENLSDFSDRPCLISGSTGKTYSFAETHLVSRKVAAGLSKLGIKKGDVIMTLLQNCPEFIFSFVGASMIGAVTTTANPFYTPSEIFKQFSASRAKLIITQSQYVTKLRDSDCCENSKTPGEDFVVITIDDPPENCLHFTVLAEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLVTSVAQQVDGENPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEMGSLLELIQKHNISVAAVVPPLVLALAKNPMVANFDLSSIRIVLSGAAPLGKELEEALRNRIPQAVLGQGYGMTEAGPVLSMCLAFSKQPLPTKSGSCGTVVRNAELKVIDPETGRSLGRNQPGEICIRGSQIMKGYLNDAEATANIIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELEALLVSHPSITDAAVVPQKDDVAGEVPVAFVVRANDLDLSEEAVKDYVAKQVVFYKKLHKVFFVHSIPKSASGKILRKDLRAKLATATTIS
MTQQTATNPIPCQFHTSKFHLSPNYSLIPCTRHDHRKFLTISPSYKTQLNFASLLPCLISTMGTPIPCSAQEEHIFQSRHPAVPVPDITLPDFVLQDAELYADKVAFVEAATGKTYTYAEVVRDTRRFAKALRSLGLRKGHVVVVALPNVAEYGIVALGIMTAGGVFSGANPTAHESELKKQAEAADAKLIVTDDLNYGKVKGLGLPVIVLGEESISTAVNWNELLDAADRAGDKLSYEEVLQSDLCALPFSSGTTGMSKGVMLTHRNLVANLCSSLFGVGPEMVGQVATLGLIPFFHIYGITGICCATLRNKGKVVVMGRFELRTFLNALITQEVNFAPIVPPIILALVKNPIVEEFDLSKLKLKAIMTAAAPLAPELLTAFENKFPGVQVQEAYGLTEHSCITLTHGDPSKGHGIAKKNSVGFLLPNLEIKFINPENGQSLPKNTPGEICVRSQCVMQGYFNNKEETARTIDADGWLHTGDIGYIDNDGDIFIVDRIKELIKYKGFQVAPAELEAILLSHPSVEDAAVVPLPDEEAGEIPAACVVMSKGAEESEEDIMEFVASNVARYKKVRVVQFVDSIPKSPSGKIMRRLLKEKMGSGSKASHFHK
>Sapur.001G029400.1.v5.1
MDTMMNPQEEFIFRSKLPDIYIPKNLPLHTYVFENLSKHSSKPCLINGANGDVYTYADVELTARRVASGLNKIGIQQGDVIMLFLPSSPEFVLAFLGASHRGAITTAANPFSTPAELAKHAKASKAKLLITQACYYEKVKDFSQENGVKIMCVDSAPDGCLHFSDLTQADENEMPQVDLHPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGENPNLYFHSEDVILCVLPMFHIYALNSIMLCGLRVGASILILPKFEIGSLLGLIEKYRVSIAPVVPPVMLAIAKSADLDKHDLSSVRMLKSGGAPLGKELEDTVRAKFPQARLGQGYGMTEAGPVLAMCLAFAKEPFDIKPGACGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYLNDPEATSRTIDKQGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLSHPEISDAAVVGMKDEDAGEVPVAFAVKSEKSQVTEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKNLREKLAGM
>Sapur.003G142800.1.v5.1
MDTMRKQKEEFIFRSKLPDIDIPKDLPLHSYVFENFSKYPSKPCLINGANGDIYTYADVELTARRVASGLNKVGIHQGDVIMLILPSSPEFVLAFLGASHRGAITTAANPFSTPAELAKQARASKSKLLITQACYYDKVKDYAQENDVKVVCVDSAPDECLHFSELTQADENDMPRAEFSPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSGDVILCVLPMFHIYALNSIMLCGLRAGAAILIMPKFEVGYLLGLIEKYKVSIAPVVPPVMLAIARSPDLDKHDLSSLRMLKSGGSPLGKELEDSVRAKFPQARLGQGYGMTEAGPVLAMCLAFAKQPFDIKPGACGTVVRNAEMKIVDPETGASLTRNQPGEICIRGDQIMKGYLNDPEATARTIDNEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLQAHPWISDAAVVGMKDEDAGEIPVAFVIKSENSQVTEEEIMQYISKQVIFYKRIKRVFFVEAIPKAPSGKILRKNLKERLAGGLQK
>Sapur.004G082800.1.v5.1
MEKSGYGRDGIYRSLRPKLVLPKDPNLSMVSFLFRNSNSYPHKPALIDADLSITLSYSELKSVVIKVAHGLLNLGISKNDVVLIFAPNSYQFPICFLAIISTGAVATTANPLYTTSELSKQIKDCNPKLIITVPELWDKVKGFNVPAVFLGPKRVPLPLESGSRIRSFDSLVELGGSSSQFPSSNVKQSDISTLLYSSGTTGVSKGVVLTHGNFIAASLMVTMDQVMAGEMHNVFLCFLPMFHVFGLAVVTYSQLQMGNAVVSMGKFEFEMVLKTIEKYRVTHMWVVPPVVLALAKQNLVKKYDLSSLRHIGSGAAPLGKDLMKECAKNLPGTIIAQGYGMTETSGIVSMEDSRLGTRHSGSAGMLAAGIEAQIVGVETLKPLPPTQLGEIWVRGLNMMKGYFNNPQATKDTIDGKGWVHTGDIGYFDEDGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPYPDAETGEVPVAYVVRSPNSALTEVDVKKFIADQVAPFKRLRKVTFVNSVPKSASGKILRRVLIEQVRSKI
>Sapur.006G138700.1.v5.1
MEAENDQAQEFIFRSKLPDIYIPSHLPLHTYCFQNLSHFKDNPCLINGSTGEIHTYADVELRSRKVASGLYKLGIKQGDVILLLLQNSPEFVFAFLGASYIGAVSTTANPFYTPAEVAKQATASKAKLIVTQAVYAEKVEQFAKENVHVRIVTIDSPPENYLHFSELTNSDENDIPAVKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCMLPLFHIYSLNSVLLCGLRVGAAILLMQKFEIITLMELVQKYKVTIAPFVPPVVLAVAKCSVVDKYDLSSIRTVMSGAAPMGKELEDAVRAKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPDTGKSLPRNQAGEICIRGCQIMKGYLNDPEATERTIDKYGWLHTGDVGYIDEDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDGAVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVVFYKRISRVFFTEEIPRAPSGKILRKDLKSRLVTGNLPY
>Sapur.017G093300.1.v5.1
MEKSGYGRDGIYRSLRPTLVLPKDSNLSLVSFLFRNSDSYPHKPALIDADLSITLSFSELKSIVIKVAHGLLNLGISKSDVVLIFAPNSYQFPICFLAITSIGAVATTANPLYTTSELSKQIKDSNPKLVITVPELWGKVKGFNLPAVFLGTKEVSLPLESGSRIRSFHSLVESGGSNSDFPASDVKQSDISTLLYSSGTTGVSKGVILTHGNFIAASLMVTMDQVTTGEMHNVFLCFLPMFHVFGLAVITYSQLQMGNAVAIMGKFEFEMVLKTIEKYRVTHMWVVPPVILALSKQSLVKKYDLSSLRNIGSGAAPLGKDLMKECAKNLPDTTITQGYGMTETSGVISVEDPRICARHSGSAGILTAGIEAQIVSVEAAVPLPPNQLGEIWVRGPNMMRGYLNNPQATKDTIDKKGWVHTGDLGYFDDDGLLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPYPDAEAGEVPVAYVVRSPNSTLSGEDIQKFIADQVAPFKRLRKVTFINTVPKSASGKILRRELVQKVKSKM
>Sapur.019G055800.1.v5.1
MMSVATVEPTKPEIPPPQSQNAPSSDETDHVFRSKLPDIPISNHLPLHTYCFENLSDFSDRPCLISGSTGKTYSFAETHLVSRKVAAGLSKLGIKKGDVIMTLLQNCPEFIFSFVGASMIGAVTTTANPFYTPSEIFKQFSASRAKLIITQSQYVTKLRDSDCCENSKTPGEDFVVITIDDPPENCLHFTVLAEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLVTSVAQQVDGENPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEMGSLLELIQKHNISVAAVVPPLVLALAKNPMVANFDLSSIRIVLSGAAPLGKELEEALRNRIPQAVLGQGYGMTEAGPVLSMCLAFSKQPLPTKSGSCGTVVRNAELKVIDPETGRSLGRNQPGEICIRGSQIMKGYLNDAEATANIIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELEALLVSHPSITDAAVVPQKDDVAGEVPVAFVVRANDLDLSEEAVKDYVAKQVVFYKKLHKVFFVHSIPKSASGKILRKDLRAKLATATTIS
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University