Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Rosmarinus officinalis
Species name
Rosemary (Salvia rosmarinus) is a species of eudicot in the family Lamiaceae (mint family).
Species info
Gene sequence info
Rosmarinus_officinalis.COMT.fa
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>1273.g240.t1
MGSTIKNPDEEENFLYAMQLASASVLPMVLKSAIELDLLELIKKAGTGAFVSPAELAAQLPTANAAAPVMLDRILRLLASYTILECRLKNLPDGGVERLYGLAPVCKFLTKNEDGVSMAPLALMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMSAFDYHGTDPRFNKVFNQGMSNHSTITMKKILETYRGFDGLKTVVDVGGGTGATLNMIVSKHPSIKGINFDLPHVIQDAPSYTGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYDALPENGKVILAECVLPEAPDTGLATKNVVHIDVIMLAHNPGGKERTEKEFHNLAKGAGFKHFNKACCAYNSWIMELLK
>1331.g263.t1
MALPNGENSSDELFDAQAHVWNYTERVPRLNHLFKEAMASDSSLVNHVVLTDCRESFQGFESLVDVSGRTGAMAKAIADAFPEMACIVLDPPHVVAG
>2606.g76.t1
MRYLIHHGLFKKTGNDHYTENALSCLFTKDKMGPFIMLHMTPPTQSDITAHVLRGGKCPDVRACDGLRPPRSSPTTRSSSRESSRWPPWHGSQLHPQGLSVHAGGCFDLPEVVTKVPHTMGSSSLAGACLKVSQKPTQSCSWYGFIQLLWFLWPWVLHDWSDSACVEILNKCKEVIPPQGKVIVVDAVVEEEDDGDEYYC
>283161.g1.t1
MLRSGALSLSSDLDSSSGDAAALLLSSPSCWLSPPIPARRRPQVARAIVAPASHPCSRNHSPRHNPFSSLPARCSPTPTATAHRYHGPSLPAPCSLLPACQRKKFLKSAIELDLLELIKMAGTGAFVSPAELAAQLPTANAAAPVMLDRILCLLASYAILECRLKNLPDGGVERLCGLAPVCKFLTKNEDGVSMAPLALMNQDKVLMDSW
>344802.g3.t1
MIISNHPSIKAINFDLPHVIQDLPSIPGVEHVGGYMFVSVPKADAIFMKWICHDWSDWHIERLLKNCYEALLENGKVIIAESILPEDPKNGLGKSWAVQVDVLMLAFNPGGKERTEMEFRTLATKAGFKQLVKVCCACDIWIMEFHK
>387383.g1.t1
TENEEGGSCCAITLMTQDKVLIDSWYHLKDAILEGGTPFNRAHGMGVFEYAAKDPKISNILNKTMSESTFLTKNIIEMYKNFEGIETVVDVGGGTGAALSIIIANNPSIKGINFDLPHVIRQAPSYPGIMHVGGDMFVNVPKGDAIFMKRVLLDWGDAECVRILRNCKAALPQNGKVVIVERLLLEIPKTGAEAEELSNVIMLAYNPGAKERTEKEFRSLAKLAGFQGFRKVCSSSATWIMEIY
>388141.g2.t1
MGSSDQNRPNLTTSLTESSSDSGFVAAMKLATGSALPMALRFAVELNLLHLIGKGGVGASASEIAAQLPAKNPHAAAMIDRVLRLLAANSILTCTMPHGGAEWRYSLAPVCKFFTANEDGVSLGPFCLLTQDKVFMDTWYHLKDAVMEGGIAFDRAHGRNAFEYTATDPRFNQVFNQAMYQHSTIFMKTILEKYNGFGGVKSLVDVGGGRGASLNMIISKHQLIKAINFDLPHVIQDLPSIPGVEHVGGDMFVSVPKADAIFMKWIFHDWSDSHVERLLKNCYEALPENGKVIIAESILPEDPKNGLGESWAVQIDVFMLAFVPGGKERTEKEFHALATKAGFKQFVKVCCACDIWIMEFHK
>576053.g3.t1
MSTEEEEARMIAIQYAYGVVLPMVLKTAVELLDLFQVIKNVESISAAELAAQLPTSNPGAAAMLDRILRLLVSNSILSCSLQDGGDERRYALSPTGKFFTKNEECRS
>576053.g4.t1
MCYIFYINPYPDQRNSDNKAKMSTEEDEARMIAIQYASGAVLPMVLKTAVELDLFQLIKNAESISAAELAAQLPTSNPGAADMLDRILRLLAAHSILTCSLHGNGVERRYALSPVGVFFTKNEDGVSCCPASLMSQEKVLMESWYHLKDAILEGGTPFNRAHGVSIFGYAAKDPRLGNILNKAMSESTFFFKKIIEMYKGFEGIQTLVDVGGGIGATLSIIISRHPSIKAINFDVPHVIQQAPSYQGIEHVGGDMFVSVPKGDVIFMKSILHNWSDAECKRILRNCRTAMAKKGKVVIVEHVLLDTPKSGVSNYVEIIDMFMLAYNPGAKERTEKEFRSLAKEAGFQGLRKVCTADALCLMELYD
>576054.g7.t1
MCYIFYINPYPDQRNSDNKAKMSTEEDEARMIAIQYASGAVLPMVLKTAVELDLFQLIKNAESISAAELAAQLLTSNPGAADMLDRILRLLAAHSILTCSLHGNGVERRYALSPVGVFFTKNEDGVSCCPASLMSQEKVLMESWYHLKDAILEGGTPFNRAHGVSIFGYAAKDPRLGNILNKAMSESTFFFKKIIEMYKGFEGIQTLVDVGGGIGATLSIIISKHPSIKAINFDVPHVIQQAPSYQGLSLSLNIFYCFKI
>576054.g6.t1
MSTEEEEARMIAIQYAYGVVLPMVLKTAVELDLFQVIKNVESISAAELAAQLPTSNPGAAAMLDRILRLLVSNSILSCSLQDGGDERRYALSPAGKFFTKNEECRSCCA
MGSTIKNPDEEENFLYAMQLASASVLPMVLKSAIELDLLELIKKAGTGAFVSPAELAAQLPTANAAAPVMLDRILRLLASYTILECRLKNLPDGGVERLYGLAPVCKFLTKNEDGVSMAPLALMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMSAFDYHGTDPRFNKVFNQGMSNHSTITMKKILETYRGFDGLKTVVDVGGGTGATLNMIVSKHPSIKGINFDLPHVIQDAPSYTGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYDALPENGKVILAECVLPEAPDTGLATKNVVHIDVIMLAHNPGGKERTEKEFHNLAKGAGFKHFNKACCAYNSWIMELLK
>1331.g263.t1
MALPNGENSSDELFDAQAHVWNYTERVPRLNHLFKEAMASDSSLVNHVVLTDCRESFQGFESLVDVSGRTGAMAKAIADAFPEMACIVLDPPHVVAG
>2606.g76.t1
MRYLIHHGLFKKTGNDHYTENALSCLFTKDKMGPFIMLHMTPPTQSDITAHVLRGGKCPDVRACDGLRPPRSSPTTRSSSRESSRWPPWHGSQLHPQGLSVHAGGCFDLPEVVTKVPHTMGSSSLAGACLKVSQKPTQSCSWYGFIQLLWFLWPWVLHDWSDSACVEILNKCKEVIPPQGKVIVVDAVVEEEDDGDEYYC
>283161.g1.t1
MLRSGALSLSSDLDSSSGDAAALLLSSPSCWLSPPIPARRRPQVARAIVAPASHPCSRNHSPRHNPFSSLPARCSPTPTATAHRYHGPSLPAPCSLLPACQRKKFLKSAIELDLLELIKMAGTGAFVSPAELAAQLPTANAAAPVMLDRILCLLASYAILECRLKNLPDGGVERLCGLAPVCKFLTKNEDGVSMAPLALMNQDKVLMDSW
>344802.g3.t1
MIISNHPSIKAINFDLPHVIQDLPSIPGVEHVGGYMFVSVPKADAIFMKWICHDWSDWHIERLLKNCYEALLENGKVIIAESILPEDPKNGLGKSWAVQVDVLMLAFNPGGKERTEMEFRTLATKAGFKQLVKVCCACDIWIMEFHK
>387383.g1.t1
TENEEGGSCCAITLMTQDKVLIDSWYHLKDAILEGGTPFNRAHGMGVFEYAAKDPKISNILNKTMSESTFLTKNIIEMYKNFEGIETVVDVGGGTGAALSIIIANNPSIKGINFDLPHVIRQAPSYPGIMHVGGDMFVNVPKGDAIFMKRVLLDWGDAECVRILRNCKAALPQNGKVVIVERLLLEIPKTGAEAEELSNVIMLAYNPGAKERTEKEFRSLAKLAGFQGFRKVCSSSATWIMEIY
>388141.g2.t1
MGSSDQNRPNLTTSLTESSSDSGFVAAMKLATGSALPMALRFAVELNLLHLIGKGGVGASASEIAAQLPAKNPHAAAMIDRVLRLLAANSILTCTMPHGGAEWRYSLAPVCKFFTANEDGVSLGPFCLLTQDKVFMDTWYHLKDAVMEGGIAFDRAHGRNAFEYTATDPRFNQVFNQAMYQHSTIFMKTILEKYNGFGGVKSLVDVGGGRGASLNMIISKHQLIKAINFDLPHVIQDLPSIPGVEHVGGDMFVSVPKADAIFMKWIFHDWSDSHVERLLKNCYEALPENGKVIIAESILPEDPKNGLGESWAVQIDVFMLAFVPGGKERTEKEFHALATKAGFKQFVKVCCACDIWIMEFHK
>576053.g3.t1
MSTEEEEARMIAIQYAYGVVLPMVLKTAVELLDLFQVIKNVESISAAELAAQLPTSNPGAAAMLDRILRLLVSNSILSCSLQDGGDERRYALSPTGKFFTKNEECRS
>576053.g4.t1
MCYIFYINPYPDQRNSDNKAKMSTEEDEARMIAIQYASGAVLPMVLKTAVELDLFQLIKNAESISAAELAAQLPTSNPGAADMLDRILRLLAAHSILTCSLHGNGVERRYALSPVGVFFTKNEDGVSCCPASLMSQEKVLMESWYHLKDAILEGGTPFNRAHGVSIFGYAAKDPRLGNILNKAMSESTFFFKKIIEMYKGFEGIQTLVDVGGGIGATLSIIISRHPSIKAINFDVPHVIQQAPSYQGIEHVGGDMFVSVPKGDVIFMKSILHNWSDAECKRILRNCRTAMAKKGKVVIVEHVLLDTPKSGVSNYVEIIDMFMLAYNPGAKERTEKEFRSLAKEAGFQGLRKVCTADALCLMELYD
>576054.g7.t1
MCYIFYINPYPDQRNSDNKAKMSTEEDEARMIAIQYASGAVLPMVLKTAVELDLFQLIKNAESISAAELAAQLLTSNPGAADMLDRILRLLAAHSILTCSLHGNGVERRYALSPVGVFFTKNEDGVSCCPASLMSQEKVLMESWYHLKDAILEGGTPFNRAHGVSIFGYAAKDPRLGNILNKAMSESTFFFKKIIEMYKGFEGIQTLVDVGGGIGATLSIIISKHPSIKAINFDVPHVIQQAPSYQGLSLSLNIFYCFKI
>576054.g6.t1
MSTEEEEARMIAIQYAYGVVLPMVLKTAVELDLFQVIKNVESISAAELAAQLPTSNPGAAAMLDRILRLLVSNSILSCSLQDGGDERRYALSPAGKFFTKNEECRSCCA
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University