Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Rosmarinus officinalis
Species name
Rosemary (Salvia rosmarinus) is a species of eudicot in the family Lamiaceae (mint family).
Species info
Gene sequence info
Rosmarinus_officinalis.ROMT.fa
Download
>1021.g49.t1
MALAFPSEVESTQDLLNAQSHVWNHIYHHINSMSLKCAIQLGIADAIHKHGKPMTLSQLVDALSINKAKTPDLARLMRVLVHSKFFNEVGDVDVDVDEEGAYTLMGASRLLLRGGPTSMAAFALAMLDPVKMDPWHHMSEWFRNNDSSPFVTKHGITIFEYAGNDQRLNALFNEAMASDAGFVTGILGKECGHVFEGVKSMVDVGGGTGAVARGVTAAFPGLKCIVLDLPHVVGGLEGSQNLSFFSGDMFEFIPRADAVFLKWLLHDWSDDDCVKILEKCKEAISPSGKVIIVEIVVDDRKQDHHATQTQLFFDLQVMVQINGKERTENEWAKLFYAAGFKNYKINPVLGLRSVIEVFP
>1021.g50.t1
MALQNGDISLPNGEVSTDELLQAQAHVWNHILTFVSSMSLKCAIQLGIPDAIHRHGKPITLSQLLESIPAHNSKSQSIRRLMRVLVQSKFFVEDGSQEEARYWLTPASRLLLREAPLTVAPLVLICLDPILTETWHHMSEWLTDERHLTQFEVAHGSTFWERIAHESNMGSLFHEAMCSDSRLLAHVLVRDYKHVFEGIKSLVDVGGGTGTMAKAIIDAMPGIKCIVLDLPHVVAGLENTDKLSYVGGDMFEAIPPANAILLKWIMHDWDDDSCLKILKRCKDAVASNGTGGKVMIVDAVVDVNREDDKVVEDQLYFDMTMMTYFNGKERSEKEWAKLFSDAGFTSYKITHAFGVRSLLEVYP
>1021.g46.t1
MALPNAVDVDSTQELLDAQTHVWNLIFSYIKPMSLKSALELGIPDVIHKHGKPMTLSQLADAFSINKAKSHGLFRLMRILVHSKIFDVSKNEDGDGDEDEEEAYSLTRASRLLLRGEPMSFAPFALATIDPIVVDTFHHVTEWCKDECPTPFFTKNGKNLWEFGGNDIKWNTLFNEGMASDAQFVGSIIVKECKQVFEGLKTVVDVGGGTGAVAKAVIDAFPGLKCVVVDLTHVVAGLEGSENLSFVSGDIFDFIPPADAVILKWILHDWSDEDCVKILEKCKAAILGSKDNGGKLIIVDMVVDCEKQEDEAFETQLFFDVLMMAGLAGKERTEKEWAKLFSAAGFTNYKITPTLGLRVRPPENRWEGGGRVDRRETDGKGEGGGWPPRKERGRELAANRGEGGLAAGRGGRDGWQPENGLAAG
>1021.g45.t1
MALPKGIESSKELLEAQAHVWNHIFNYINSMSLKCAIELGIADVIHKHDKPMTLSQLADALSINKAKSNALFRLMRILVHSKFFDKVKTLSEYEDEDEDEDEEEAYCLTQASRLLLRDEPLSMATFAVAMIDPIFVDPFYHVSKWFGDDHPSPFFTKNGKNLWEIAETEQRSNQLINEAMACDARLVSAILVQECKQVFEGLGTVVDVGGGTGMVARGVAAAFPGLKCVVLDLPHVVAGLEGSENLTFVGGDMFESIPHADAVFLKWILHDWSDEDCVRILEKCKEAIAPSNNNGGKVIIVDMILDDKIEKDEATETQLLIDGMMMAVVTGRERTEKEWAKLFFAAGFQKYNITHVLGLRSVIEVSP
>1021.g48.t1
MALPNQVDSTQELLDAQTHVWNLIFSYIKPMCLKSALELGIPDVIHKHGKPITLSQLADALSINKAKSHGLFSLMRILVHSKIFEKVNISKDEDEEEAYSLTRASRLLLRDEPLSFAPFALAMIDPIMADPFYNVTEWFKDECPSPFVTRNGRNIWEFFSGNDRNFNQVFNEGMASDAQFVGSILVEKCKHVFKGLKTVVDVGGGTGTVAKVVADAFSGLKCTVLELPHVVAGLEGSENLKFVCGDMFEFIPPANAVFLKWILHDWGDEESVKILQKCKEAILGSKEKGGKVIIVEMVVDCENQEDEAIETQLCMDMLMMTEVTGKERSEKEWAKLFSAAGFNNYKITPILGLRETTSPTIQAPTTALLTVATPDRRDDCATLPQPPAATILLHCYLKVGDVHVRLRSSRKERLREKQRQNGVPDQAAARRLWAERQRETARGGGGRPLGEEEGWRWGLAARRHHNSKLSSPKMALASKELLDAQSHVWNHIYHYINSMSLKCAIQLGIPDAIHEHGNPVTLSQLADALSINVAKSDALRRLMRILVHCKFFDLVKVRVKDEDEAYCLTGASRLLLRHGPLSMVPFALLMLDPVSTDPWHSMSDWFRDECSSSFETKHGTTFWEYAVGDASLNVLFNEGMASDAVFVTSVLCKDSRHVFEGLRSMVDVGGGTGATAKGFAAAFPELKCVVLDLPHVVGGLEGSENLRFVGGDMFESIPRADAVFLKWVLHDWSDEDCVKILENCKEAINVNGGKVILVELVVEDRVEEHQATETQVFFDMQMMTVINGKDRNEKEWAKLFFAAGFGNYKITPVLGLRSVIEVFP
>1021.g47.t1
MALPIQVDSTQELLDAQTHVWNLIFSYIKPMSLKSALELGIPDVIHKHGKPMTLSQLADAISINKAKSHGLFRLMRILVHSKIFEKVNISKDEDQDEEEAYSLTRASRLLLRDEPLSFAPFALAMIDPIIADPFHNVTEWFKDECPSPFVTRNGRNLWEYFSGNDHRNFNQVFNEGMASDAQFVGSILVEKCKHVFEGLKTVVDVGGGTGTVAKVVADAFSGLKCTVLELPHVVAGLEGSENLNFVSGDMFEFIPPADAVFLKWIFHDWGDEDSVKILKKCKESILGSKEKGGKVIIVEMVVDSENQENEAIETQLYMDMLMMTEVTGKERSEKEWAKLFSAAGFNNYKITPILGLRSLSLSLMFVGGDMFESIPHADAVFLKWVLHDWSDVDCVKILAKCKEAINVKVGKVILVELVVEDRVEEHQATETQAFFDMQMMIEINGRERSEKEWTKLFFAAGFRNYKITPVLRLRSVIEVFP
>1331.g264.t1
MTYVEGDMFEAIPRADLVLVKSILHDWNGEDSIRILKKCREAIGRSKQRDGKAMIIDMILNNHGGGDKAIEDQLLYDTAMMTYLNGKERDEKECGDVHRCRIQWLQDYS
>1331.g263.t1
MALPNGENSSDELFDAQAHVWNYTERVPRLNHLFKEAMASDSSLVNHVVLTDCRESFQGFESLVDVSGRTGAMAKAIADAFPEMACIVLDPPHVVAG
>1465.g36.t1
MALPNEVDQSTQDLLNAQSHVLNHIYHYINSMSLKCAIQLGIPDAIHNHNKPITLSQLADALSINKAKTPDLARLLRVLEHSRFLNKGEDGAYSLTGASHLLLRRGPMSMAAFALAVLDPVKFDPYHHMSEWFQNDDSSPFLTKHGVTLFEYAGNDPRLNALFNEAMASDAGVVTSVLGRECGHVFEGVKSMVEVGGGTGAAARGVAAAFPGLKCIVLDLPHVVGGLEGSENLSFFSGDMFDFIPQADAVFLKWILHDWSDEDCLIILGKCKDAIGAGGKVIIVDVVVDDQKHDHLTTETQLFFDLHMMVQVVGKERTEKEWANLFFAAGFKNYKINHVLGWRSVIEVFP
>1465.g37.t1
MALATGFGQDLLDSETHVRNHIFNYINSMSLKCAIQLGIPDAIHKHGKPMSFSQLADALPINGAKSDALRRLMRILVHSKFFDKVKISEDEEEAYCLTGPSRLLLRCGPLSMVPLALIIFDPILMDPWHHMSDWFRDECSSSFETKHGTTFWEYAKGDATLNVLFNEGMASDAVFVATILGKECGHVFQGVKSMVDVGGGTGALARGVAAAFPELKCVVLDLPHVVDGLEGSENLKFVGGDMFESIPQADAIFLKWILHDWSDEDCVKILKNCKEAITPSKINGGKVIIVEIVVYDRKEDIEATETQIFSDMHMMAELTGKERTEKEWATIFFAAGFGNYKITPALGLRSVIEVFP
>1465.g38.t1
MAFANGFKQDLLGSQTHVWNHIFNYINSMSLKCAIRLGIPDAIHTHGKPMTLSQLASALSINGAKSDALRRLMRILVHSKFFDKVRIFEEDAYCLTGASRLLLRREPLSMAPFALVMLDPVSTDPWHRMSEWFRDERSSSFETEHGLTFWEFAEGDGRLDVLFNEGMASDAGCVTSVLCRDARHVFKGLESIVDVGGGTGATAKGFATAFPGLKCVVLDLPRVVAGLEGSENLRERSEKEWAKLFFAAHFGSYKITPVLGLRSVIEVFP
>2535.g17.t1
MSLKCPIEPDIPDIIQPMTRSHLVDALSINPAKSRHLLRLMRVLVHSHFFIKVDISDDEGYWLTPASRLLLKHNPFCALPFSCVVVNHLMQEPWHHMSAWLTGEHHLTPFETVHGMTFWDYTEHCREAFGGFNPLVDVGGSAGAMAKAEANAYAFPEMA
MALAFPSEVESTQDLLNAQSHVWNHIYHHINSMSLKCAIQLGIADAIHKHGKPMTLSQLVDALSINKAKTPDLARLMRVLVHSKFFNEVGDVDVDVDEEGAYTLMGASRLLLRGGPTSMAAFALAMLDPVKMDPWHHMSEWFRNNDSSPFVTKHGITIFEYAGNDQRLNALFNEAMASDAGFVTGILGKECGHVFEGVKSMVDVGGGTGAVARGVTAAFPGLKCIVLDLPHVVGGLEGSQNLSFFSGDMFEFIPRADAVFLKWLLHDWSDDDCVKILEKCKEAISPSGKVIIVEIVVDDRKQDHHATQTQLFFDLQVMVQINGKERTENEWAKLFYAAGFKNYKINPVLGLRSVIEVFP
>1021.g50.t1
MALQNGDISLPNGEVSTDELLQAQAHVWNHILTFVSSMSLKCAIQLGIPDAIHRHGKPITLSQLLESIPAHNSKSQSIRRLMRVLVQSKFFVEDGSQEEARYWLTPASRLLLREAPLTVAPLVLICLDPILTETWHHMSEWLTDERHLTQFEVAHGSTFWERIAHESNMGSLFHEAMCSDSRLLAHVLVRDYKHVFEGIKSLVDVGGGTGTMAKAIIDAMPGIKCIVLDLPHVVAGLENTDKLSYVGGDMFEAIPPANAILLKWIMHDWDDDSCLKILKRCKDAVASNGTGGKVMIVDAVVDVNREDDKVVEDQLYFDMTMMTYFNGKERSEKEWAKLFSDAGFTSYKITHAFGVRSLLEVYP
>1021.g46.t1
MALPNAVDVDSTQELLDAQTHVWNLIFSYIKPMSLKSALELGIPDVIHKHGKPMTLSQLADAFSINKAKSHGLFRLMRILVHSKIFDVSKNEDGDGDEDEEEAYSLTRASRLLLRGEPMSFAPFALATIDPIVVDTFHHVTEWCKDECPTPFFTKNGKNLWEFGGNDIKWNTLFNEGMASDAQFVGSIIVKECKQVFEGLKTVVDVGGGTGAVAKAVIDAFPGLKCVVVDLTHVVAGLEGSENLSFVSGDIFDFIPPADAVILKWILHDWSDEDCVKILEKCKAAILGSKDNGGKLIIVDMVVDCEKQEDEAFETQLFFDVLMMAGLAGKERTEKEWAKLFSAAGFTNYKITPTLGLRVRPPENRWEGGGRVDRRETDGKGEGGGWPPRKERGRELAANRGEGGLAAGRGGRDGWQPENGLAAG
>1021.g45.t1
MALPKGIESSKELLEAQAHVWNHIFNYINSMSLKCAIELGIADVIHKHDKPMTLSQLADALSINKAKSNALFRLMRILVHSKFFDKVKTLSEYEDEDEDEDEEEAYCLTQASRLLLRDEPLSMATFAVAMIDPIFVDPFYHVSKWFGDDHPSPFFTKNGKNLWEIAETEQRSNQLINEAMACDARLVSAILVQECKQVFEGLGTVVDVGGGTGMVARGVAAAFPGLKCVVLDLPHVVAGLEGSENLTFVGGDMFESIPHADAVFLKWILHDWSDEDCVRILEKCKEAIAPSNNNGGKVIIVDMILDDKIEKDEATETQLLIDGMMMAVVTGRERTEKEWAKLFFAAGFQKYNITHVLGLRSVIEVSP
>1021.g48.t1
MALPNQVDSTQELLDAQTHVWNLIFSYIKPMCLKSALELGIPDVIHKHGKPITLSQLADALSINKAKSHGLFSLMRILVHSKIFEKVNISKDEDEEEAYSLTRASRLLLRDEPLSFAPFALAMIDPIMADPFYNVTEWFKDECPSPFVTRNGRNIWEFFSGNDRNFNQVFNEGMASDAQFVGSILVEKCKHVFKGLKTVVDVGGGTGTVAKVVADAFSGLKCTVLELPHVVAGLEGSENLKFVCGDMFEFIPPANAVFLKWILHDWGDEESVKILQKCKEAILGSKEKGGKVIIVEMVVDCENQEDEAIETQLCMDMLMMTEVTGKERSEKEWAKLFSAAGFNNYKITPILGLRETTSPTIQAPTTALLTVATPDRRDDCATLPQPPAATILLHCYLKVGDVHVRLRSSRKERLREKQRQNGVPDQAAARRLWAERQRETARGGGGRPLGEEEGWRWGLAARRHHNSKLSSPKMALASKELLDAQSHVWNHIYHYINSMSLKCAIQLGIPDAIHEHGNPVTLSQLADALSINVAKSDALRRLMRILVHCKFFDLVKVRVKDEDEAYCLTGASRLLLRHGPLSMVPFALLMLDPVSTDPWHSMSDWFRDECSSSFETKHGTTFWEYAVGDASLNVLFNEGMASDAVFVTSVLCKDSRHVFEGLRSMVDVGGGTGATAKGFAAAFPELKCVVLDLPHVVGGLEGSENLRFVGGDMFESIPRADAVFLKWVLHDWSDEDCVKILENCKEAINVNGGKVILVELVVEDRVEEHQATETQVFFDMQMMTVINGKDRNEKEWAKLFFAAGFGNYKITPVLGLRSVIEVFP
>1021.g47.t1
MALPIQVDSTQELLDAQTHVWNLIFSYIKPMSLKSALELGIPDVIHKHGKPMTLSQLADAISINKAKSHGLFRLMRILVHSKIFEKVNISKDEDQDEEEAYSLTRASRLLLRDEPLSFAPFALAMIDPIIADPFHNVTEWFKDECPSPFVTRNGRNLWEYFSGNDHRNFNQVFNEGMASDAQFVGSILVEKCKHVFEGLKTVVDVGGGTGTVAKVVADAFSGLKCTVLELPHVVAGLEGSENLNFVSGDMFEFIPPADAVFLKWIFHDWGDEDSVKILKKCKESILGSKEKGGKVIIVEMVVDSENQENEAIETQLYMDMLMMTEVTGKERSEKEWAKLFSAAGFNNYKITPILGLRSLSLSLMFVGGDMFESIPHADAVFLKWVLHDWSDVDCVKILAKCKEAINVKVGKVILVELVVEDRVEEHQATETQAFFDMQMMIEINGRERSEKEWTKLFFAAGFRNYKITPVLRLRSVIEVFP
>1331.g264.t1
MTYVEGDMFEAIPRADLVLVKSILHDWNGEDSIRILKKCREAIGRSKQRDGKAMIIDMILNNHGGGDKAIEDQLLYDTAMMTYLNGKERDEKECGDVHRCRIQWLQDYS
>1331.g263.t1
MALPNGENSSDELFDAQAHVWNYTERVPRLNHLFKEAMASDSSLVNHVVLTDCRESFQGFESLVDVSGRTGAMAKAIADAFPEMACIVLDPPHVVAG
>1465.g36.t1
MALPNEVDQSTQDLLNAQSHVLNHIYHYINSMSLKCAIQLGIPDAIHNHNKPITLSQLADALSINKAKTPDLARLLRVLEHSRFLNKGEDGAYSLTGASHLLLRRGPMSMAAFALAVLDPVKFDPYHHMSEWFQNDDSSPFLTKHGVTLFEYAGNDPRLNALFNEAMASDAGVVTSVLGRECGHVFEGVKSMVEVGGGTGAAARGVAAAFPGLKCIVLDLPHVVGGLEGSENLSFFSGDMFDFIPQADAVFLKWILHDWSDEDCLIILGKCKDAIGAGGKVIIVDVVVDDQKHDHLTTETQLFFDLHMMVQVVGKERTEKEWANLFFAAGFKNYKINHVLGWRSVIEVFP
>1465.g37.t1
MALATGFGQDLLDSETHVRNHIFNYINSMSLKCAIQLGIPDAIHKHGKPMSFSQLADALPINGAKSDALRRLMRILVHSKFFDKVKISEDEEEAYCLTGPSRLLLRCGPLSMVPLALIIFDPILMDPWHHMSDWFRDECSSSFETKHGTTFWEYAKGDATLNVLFNEGMASDAVFVATILGKECGHVFQGVKSMVDVGGGTGALARGVAAAFPELKCVVLDLPHVVDGLEGSENLKFVGGDMFESIPQADAIFLKWILHDWSDEDCVKILKNCKEAITPSKINGGKVIIVEIVVYDRKEDIEATETQIFSDMHMMAELTGKERTEKEWATIFFAAGFGNYKITPALGLRSVIEVFP
>1465.g38.t1
MAFANGFKQDLLGSQTHVWNHIFNYINSMSLKCAIRLGIPDAIHTHGKPMTLSQLASALSINGAKSDALRRLMRILVHSKFFDKVRIFEEDAYCLTGASRLLLRREPLSMAPFALVMLDPVSTDPWHRMSEWFRDERSSSFETEHGLTFWEFAEGDGRLDVLFNEGMASDAGCVTSVLCRDARHVFKGLESIVDVGGGTGATAKGFATAFPGLKCVVLDLPRVVAGLEGSENLRERSEKEWAKLFFAAHFGSYKITPVLGLRSVIEVFP
>2535.g17.t1
MSLKCPIEPDIPDIIQPMTRSHLVDALSINPAKSRHLLRLMRVLVHSHFFIKVDISDDEGYWLTPASRLLLKHNPFCALPFSCVVVNHLMQEPWHHMSAWLTGEHHLTPFETVHGMTFWDYTEHCREAFGGFNPLVDVGGSAGAMAKAEANAYAFPEMA
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University