Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Rorippa islandica
Species name
Rorippa islandica is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Rorippa_islandica.FLS.fa
Download
>Roisl.0017s0063.1.v1.1
MVLQRDQDDISSSHVPVVDLSNPDQELVARAVVKASEEWGVFQVVNHGIPTKVIQRLQNVGRQFFELPEVEKNAVAKLADGREGYARRYELNLESKIGTLDQLFHNIWPPSSVNYKYWPRNPLDYREANEEYTRHVKMLSEKIMKWLSEGLGY
>Roisl.0017s0064.1.v1.1
MIVNYYPPCPHSDLIQGLDPHTDVSGLTLLLPNEIPGLQVFKDDHWFDLENITSAVIVIIGDQIQRMSNGRYNNVLHKTIVDKERTRMSWPVLVRPTMDMVVGPFTEITGHDDPPKFKPIAYKDYIYRKLRHLSLAGFDL
>Roisl.0024s0237.1.v1.1
MAARSYRRKLWNVYQEGLGLRREALKEGLGGETVEYLMKINYYPPSPDPDLVVGAPEHTHVMESHS
>Roisl.0024s0238.1.v1.1
MEDERDHTTSSLCKQLANSTLQGSPNVPVVDLSVSDEEFLMRAVTKASEEWGIFQVVNHGIPVELMRQLQVVGKQFFELPAAEKEAVAKAEDFEGYKRNYLEGIKNWDEHLFHRVSPPSIINYKYWPKNPPQYREVNEEYTKQVKKLTEKLLGWLSEGLGLPSMALTQSLGGETAEYVIRINYYPPSPQSELAIGAAEHTDTGAITLLVPNDVPGLQAFKDGHWFDLDYINSAVVVIIGDQLMRMTNGRFKNVFHRAKSHKEKMRISWPVFVGPRADMSVGPLPELTGDENPPKFETLVYKDYLLQKIKNWTLNKLPLY
>Roisl.0024s0239.1.v1.1
MEVPVVDLSVTDEEFLVHAVTKASEEWGIFQIVNHGIPEELMQQLKETGKQFFELPEAEKETVAKEEDFEGFKRNYLGGIKNWDEHLFHRLSPPSIINYKYWPKNPPQYREVTEEYTKHMQRLTDKILGLLSEGLGLPRVTLTKSIGEDTAEYVLRINFYPPTQHSELTIGAAPHTDMGAIAFLIPNEVPGLQAFKDEQWLDLNYINSAVVVIIGDQLMRMTNGRFKNVFHRAKTDKEKLRISWPVFVAPRDDMLVGPLPELTGDENPPKFETLIYKDYIDQKIRGWALEDLPVYGKPKDK
>Roisl.0041s0024.1.v1.1
MTKTGIKSTLSPAISLQHTKTNTKKQSFLSRIVMELPVVDLSRYLEYSGDQLGTDLLDSCREVSRILRETGALLIKDPRCSAQDNDRFIDMMETFFQMPDDFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKLKTMPNEYKPHIPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPEGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLKNGEKVAVKVPVGCLLIQTGKQIEWLTGGECIAGMHEVVVTSKTKDAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFVESSLASNYSAIPAGEYVEQELSVINLKGKKGFS
>Roisl.0052s0062.1.v1.1
MEKPKFKTVQEVVAAGEEPPKRYLYPPTNDGEDQLLAGFVPEMEIPVIDLSILLSSSDEGREELSKLHSALSTLGVVQVMNHAITEALLVKIYELTKQFFKLSSEEKQKYEREIGDFQGYGNDMIIYDDQVLDWIDRLYLITYPEDQRQLKFWPKIPIGFREYLHEYTMKQRLVVEKIFKALARSLELEDNCFQEMCGENATMETRFNMYPPCSRSDKVLGFKPHADGSAFTLLLPDKNVGGLQFLKDGKWYKAPLLPHTILIIIGDMIEIMSNGIYKSPVHRAVTNKEKERISVATFCNADPDKEIQPVDRLVSEARPKLYKTIKKYMEIYFDYYQQSRRPIEAALI
>Roisl.0210s0616.1.v1.1
MEFERVQDIASSSLLTEAIPLEFIRSEKDQPAITTFRGPTPAIPVVDLSDPDEESVMRAVVKASEEWGIFQVVNHGIPTELIRRLQDVGRKFFELPSTEKESVAKPEDSKDIEGYGTKLQKDLEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAIHVKKLSETLLGLLSEGLGLKREALREGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGKYKNVLHRTTVDKEKTRMSWPVFLEPHREMIVGPLPELTGDNNPPKFKPFAFKDYSYRKLNKLPMD
MVLQRDQDDISSSHVPVVDLSNPDQELVARAVVKASEEWGVFQVVNHGIPTKVIQRLQNVGRQFFELPEVEKNAVAKLADGREGYARRYELNLESKIGTLDQLFHNIWPPSSVNYKYWPRNPLDYREANEEYTRHVKMLSEKIMKWLSEGLGY
>Roisl.0017s0064.1.v1.1
MIVNYYPPCPHSDLIQGLDPHTDVSGLTLLLPNEIPGLQVFKDDHWFDLENITSAVIVIIGDQIQRMSNGRYNNVLHKTIVDKERTRMSWPVLVRPTMDMVVGPFTEITGHDDPPKFKPIAYKDYIYRKLRHLSLAGFDL
>Roisl.0024s0237.1.v1.1
MAARSYRRKLWNVYQEGLGLRREALKEGLGGETVEYLMKINYYPPSPDPDLVVGAPEHTHVMESHS
>Roisl.0024s0238.1.v1.1
MEDERDHTTSSLCKQLANSTLQGSPNVPVVDLSVSDEEFLMRAVTKASEEWGIFQVVNHGIPVELMRQLQVVGKQFFELPAAEKEAVAKAEDFEGYKRNYLEGIKNWDEHLFHRVSPPSIINYKYWPKNPPQYREVNEEYTKQVKKLTEKLLGWLSEGLGLPSMALTQSLGGETAEYVIRINYYPPSPQSELAIGAAEHTDTGAITLLVPNDVPGLQAFKDGHWFDLDYINSAVVVIIGDQLMRMTNGRFKNVFHRAKSHKEKMRISWPVFVGPRADMSVGPLPELTGDENPPKFETLVYKDYLLQKIKNWTLNKLPLY
>Roisl.0024s0239.1.v1.1
MEVPVVDLSVTDEEFLVHAVTKASEEWGIFQIVNHGIPEELMQQLKETGKQFFELPEAEKETVAKEEDFEGFKRNYLGGIKNWDEHLFHRLSPPSIINYKYWPKNPPQYREVTEEYTKHMQRLTDKILGLLSEGLGLPRVTLTKSIGEDTAEYVLRINFYPPTQHSELTIGAAPHTDMGAIAFLIPNEVPGLQAFKDEQWLDLNYINSAVVVIIGDQLMRMTNGRFKNVFHRAKTDKEKLRISWPVFVAPRDDMLVGPLPELTGDENPPKFETLIYKDYIDQKIRGWALEDLPVYGKPKDK
>Roisl.0041s0024.1.v1.1
MTKTGIKSTLSPAISLQHTKTNTKKQSFLSRIVMELPVVDLSRYLEYSGDQLGTDLLDSCREVSRILRETGALLIKDPRCSAQDNDRFIDMMETFFQMPDDFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKLKTMPNEYKPHIPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPEGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLKNGEKVAVKVPVGCLLIQTGKQIEWLTGGECIAGMHEVVVTSKTKDAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFVESSLASNYSAIPAGEYVEQELSVINLKGKKGFS
>Roisl.0052s0062.1.v1.1
MEKPKFKTVQEVVAAGEEPPKRYLYPPTNDGEDQLLAGFVPEMEIPVIDLSILLSSSDEGREELSKLHSALSTLGVVQVMNHAITEALLVKIYELTKQFFKLSSEEKQKYEREIGDFQGYGNDMIIYDDQVLDWIDRLYLITYPEDQRQLKFWPKIPIGFREYLHEYTMKQRLVVEKIFKALARSLELEDNCFQEMCGENATMETRFNMYPPCSRSDKVLGFKPHADGSAFTLLLPDKNVGGLQFLKDGKWYKAPLLPHTILIIIGDMIEIMSNGIYKSPVHRAVTNKEKERISVATFCNADPDKEIQPVDRLVSEARPKLYKTIKKYMEIYFDYYQQSRRPIEAALI
>Roisl.0210s0616.1.v1.1
MEFERVQDIASSSLLTEAIPLEFIRSEKDQPAITTFRGPTPAIPVVDLSDPDEESVMRAVVKASEEWGIFQVVNHGIPTELIRRLQDVGRKFFELPSTEKESVAKPEDSKDIEGYGTKLQKDLEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAIHVKKLSETLLGLLSEGLGLKREALREGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGKYKNVLHRTTVDKEKTRMSWPVFLEPHREMIVGPLPELTGDNNPPKFKPFAFKDYSYRKLNKLPMD
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University