Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Rhodiola crenulata
Species name
Da hua hong jing tian (Rhodiola crenulata) is a species of eudicot in the family Crassulaceae (stonecrop family).
Species info
Gene sequence info
Rhodiola_crenulata.COMT.fa
Download
>CCG020913.1
MEGGGVPFDREYGMNLFEYLGTDSRFNQVFNKAMSSHTTLLMKKVLGVYKGFQGLKVLVDVGGGVGVTLGMITAKYPNIKGNRYNDEHCLKLLKNCYGATPHSGKVIIVESILPDVPDDHLTTNIACEHDLLMLAQTPGGKERRLNEYDALAKGAGFSGCQVVDNAYNSWIMEFTK
>CCG020920.1
MYIEYQSINKVFCTEDELEKPAIPKMSIPIPIPNHRTEPSDELAKQAIRLANALVLPMALKSAIELNVIKVLSHAGPEAFLSPSQIAVEIGATRNPNAPVLLDRVLNLLASHSILKCRGKTDENGVVERVYGVSPICEFLTEDEDGGSMAPLFLLHHDKVFTKSWYHLNDVIMEGSGGVPFDREYGMNVFEYLGTDPRFNQVFNKAMSSHTTLLMKKVLSVYKGFQGVKVLVDVGGGVGVTLGMITAKYPNIKGINFDLHHVLEVAPSYPGVEHISGDMFSKIPKGDAIFMKWMLHGWGDEHCLKLLNNCYEALPRSGKAIIVESILPDVPDDHLTTNIACEQDMFMLAQTPGGKERRLNEYDALAKGAGFSGCQVVGNAYNSWIMEFTK
>CCG020919.1
MSSTVPNSKTEPSDELAKQAIRLANAVVLPMAIKSAIELNVIKVLSRAGPEAFLSPSQIAAEIGATRNPNAPVLLDRVLNVLASHSILKCYGKTGVEHIGGDMFSEVPKGDAIFMKWMLHGWSDEHCLKLLKNCYEALPRSGMAIIVESILPDVPDDHLTTNIACEHDLLMLAQTPGGKERRLNEYDALAKGAGFSGCQVVDNAYNSWIMEFTK
>CCG020915.1
MSSSVPNSKTERSDATDELAKQAIRLANAVVLTMALKSAIELNVINVLSRAGPEAFLTPSQIAVEIGATRNPNAPILLDRVLNLLASYSILKCCGKTGEDGVVERVYGVSPICQFLTENEDGRSVAPLLLLHHDNVVMKSWYHLNDVIMEGEGGVPFDREYGMNLFEYLGTDPRFNQVFNKAMSSHTTLIMKKVLGVYRGFECVKVLVDVGGGVGVTLGMITAKYPNIKGINFDLPHVLEVAPSFPGVEHIGGDMFSEVPKGDAIFMKWILHDWSDEHCLKLLKNCYEALPHSGKVIIVESILPDVPDDHLTTNIACELDMLMLAHTTGGKERRLNAYDALAKGAGFSGCLVVVDAYDSWIMEFTK
>CCG026840.1
MMSVSTPFAYIAVQMALKAAIELNVFDILAESGTQLSAPQIASKIPTTNPDQAIPALHRILRFLSSNSFLTTSLPLDSTIDDERDRLFGLTGLTASTLVRIDGADSLPMIGSLLLLHSQKEMFECLDKLKYTVLDPERAKCPFEMAHGVECYDYLAQRPGSQFNMLFDRTMSGSYKFFCDSLKRVYRGFDDVIQLMDVGGNDGSGLEYIISLHPQIKSCINFDLPGVIARAPYREGVKHVAGDMFVALPSCKTIMLKWILHNWSDQECEKLLQNCWRALPEGGKVISVEMMIPDNLEMSSETVMAITFDFIMLASFTGGKQRTLSEFRKLATAAGFSDVVNVVPIDNGMHVLEFLK
>CCG004258.1
MSVSTPFAYIAVQMALKAAIELNIFDILAQSGPPGTQLSAPQIASKIPTTNPHQAIPALHRILRFLSSNSFLTTSLPLDSTIDDERDRLFGLTGMTASTLARSDGADSLPMIGLFLLHQSQKEMFECFDKLKYAVLDPERAKCPFEMAHGVEWYDYLAQRPGSQFNMLFDRIMSGSYNFFCDSLMRVYRGFDDLIELMDVGGNDGSGLEYIISLHPQIKSCINFDLPGVIARAPYRKGLKHVAGDMFEALPSSKTIMLKLILHNWSDQECEKLLQNCWRALPESGKVIAVEMMIPDNLERNSAETMAITFGFIMLACFTGKERTLSEFRKLATAAGFSDVVNVVPLENGMHVLEFLK
>CCG010992.1
MAHGVGLYDYLAQRPNSELTMLFDQLMGSSIKPFFDSVIRVYSGFEEVTELMDVGGNHGSALQQIISIYPNIKGCINFDLPHVISRAPPLKGTT
>CCG016630.1
MGAAPEKALFDLSPAEEEEANKFAMQLTSFSVLPMILKSAIELDLLEIIAKAGPGAYLSAKEIASQLPATNPDAGMMLDRMLRFLACYSVVKCSFRKGGEGKIERVYGLAPVCKFLVKNEDGVAISALALMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMTAFDYHGTDPRFNKVFNKGMADHSTITMKKILETYDGFNGLTSVVDVGGGTGAVLNMIVSKCPNIKGINFDLPHVVQDAPSYPGTF
>CCG016104.1
MADETSAARNDARLAILELANMISVPMSLNAVVHLNIPNAIWQHGANSPLTASQILSLLPPSSSSAPPDPENLQRILRMLTSYNVFNEHVDSLTSSRSYSLTEVGQTLITDSDGLSYGAYVLQHHQDALMKAWPLVHEAVLDPTTEPFVKANGEAAYDYYGKKPEMNELMLKAMSGVSVPFMKAILDGYDGFEGVRTLVDVGGSAGDCLKMILRKYPGVVEGINFDLPEVVAKPPPIAGVRHVGGDMFKSIPSGDAIFMKWVLTTWTDDECRLIMENCYKALPTGGKLIACEPVLPEESDDSHRTRALLEGDIFVMTIYRAKGKHRTEDEFKKLGILAGFPQFRAFYIDHFYTVLEFQK
>CCG029380.1
MFESVPSADAIFMKWILHDWSDAHCLKLLKNCYKALPDNGIVIVVDANLPDVPDNSSTTKSISQMDVLMLAQNPGGKERRAHELLALAKEAGFSGIRHVGCYCLFWVMEFYKCNI
MEGGGVPFDREYGMNLFEYLGTDSRFNQVFNKAMSSHTTLLMKKVLGVYKGFQGLKVLVDVGGGVGVTLGMITAKYPNIKGNRYNDEHCLKLLKNCYGATPHSGKVIIVESILPDVPDDHLTTNIACEHDLLMLAQTPGGKERRLNEYDALAKGAGFSGCQVVDNAYNSWIMEFTK
>CCG020920.1
MYIEYQSINKVFCTEDELEKPAIPKMSIPIPIPNHRTEPSDELAKQAIRLANALVLPMALKSAIELNVIKVLSHAGPEAFLSPSQIAVEIGATRNPNAPVLLDRVLNLLASHSILKCRGKTDENGVVERVYGVSPICEFLTEDEDGGSMAPLFLLHHDKVFTKSWYHLNDVIMEGSGGVPFDREYGMNVFEYLGTDPRFNQVFNKAMSSHTTLLMKKVLSVYKGFQGVKVLVDVGGGVGVTLGMITAKYPNIKGINFDLHHVLEVAPSYPGVEHISGDMFSKIPKGDAIFMKWMLHGWGDEHCLKLLNNCYEALPRSGKAIIVESILPDVPDDHLTTNIACEQDMFMLAQTPGGKERRLNEYDALAKGAGFSGCQVVGNAYNSWIMEFTK
>CCG020919.1
MSSTVPNSKTEPSDELAKQAIRLANAVVLPMAIKSAIELNVIKVLSRAGPEAFLSPSQIAAEIGATRNPNAPVLLDRVLNVLASHSILKCYGKTGVEHIGGDMFSEVPKGDAIFMKWMLHGWSDEHCLKLLKNCYEALPRSGMAIIVESILPDVPDDHLTTNIACEHDLLMLAQTPGGKERRLNEYDALAKGAGFSGCQVVDNAYNSWIMEFTK
>CCG020915.1
MSSSVPNSKTERSDATDELAKQAIRLANAVVLTMALKSAIELNVINVLSRAGPEAFLTPSQIAVEIGATRNPNAPILLDRVLNLLASYSILKCCGKTGEDGVVERVYGVSPICQFLTENEDGRSVAPLLLLHHDNVVMKSWYHLNDVIMEGEGGVPFDREYGMNLFEYLGTDPRFNQVFNKAMSSHTTLIMKKVLGVYRGFECVKVLVDVGGGVGVTLGMITAKYPNIKGINFDLPHVLEVAPSFPGVEHIGGDMFSEVPKGDAIFMKWILHDWSDEHCLKLLKNCYEALPHSGKVIIVESILPDVPDDHLTTNIACELDMLMLAHTTGGKERRLNAYDALAKGAGFSGCLVVVDAYDSWIMEFTK
>CCG026840.1
MMSVSTPFAYIAVQMALKAAIELNVFDILAESGTQLSAPQIASKIPTTNPDQAIPALHRILRFLSSNSFLTTSLPLDSTIDDERDRLFGLTGLTASTLVRIDGADSLPMIGSLLLLHSQKEMFECLDKLKYTVLDPERAKCPFEMAHGVECYDYLAQRPGSQFNMLFDRTMSGSYKFFCDSLKRVYRGFDDVIQLMDVGGNDGSGLEYIISLHPQIKSCINFDLPGVIARAPYREGVKHVAGDMFVALPSCKTIMLKWILHNWSDQECEKLLQNCWRALPEGGKVISVEMMIPDNLEMSSETVMAITFDFIMLASFTGGKQRTLSEFRKLATAAGFSDVVNVVPIDNGMHVLEFLK
>CCG004258.1
MSVSTPFAYIAVQMALKAAIELNIFDILAQSGPPGTQLSAPQIASKIPTTNPHQAIPALHRILRFLSSNSFLTTSLPLDSTIDDERDRLFGLTGMTASTLARSDGADSLPMIGLFLLHQSQKEMFECFDKLKYAVLDPERAKCPFEMAHGVEWYDYLAQRPGSQFNMLFDRIMSGSYNFFCDSLMRVYRGFDDLIELMDVGGNDGSGLEYIISLHPQIKSCINFDLPGVIARAPYRKGLKHVAGDMFEALPSSKTIMLKLILHNWSDQECEKLLQNCWRALPESGKVIAVEMMIPDNLERNSAETMAITFGFIMLACFTGKERTLSEFRKLATAAGFSDVVNVVPLENGMHVLEFLK
>CCG010992.1
MAHGVGLYDYLAQRPNSELTMLFDQLMGSSIKPFFDSVIRVYSGFEEVTELMDVGGNHGSALQQIISIYPNIKGCINFDLPHVISRAPPLKGTT
>CCG016630.1
MGAAPEKALFDLSPAEEEEANKFAMQLTSFSVLPMILKSAIELDLLEIIAKAGPGAYLSAKEIASQLPATNPDAGMMLDRMLRFLACYSVVKCSFRKGGEGKIERVYGLAPVCKFLVKNEDGVAISALALMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMTAFDYHGTDPRFNKVFNKGMADHSTITMKKILETYDGFNGLTSVVDVGGGTGAVLNMIVSKCPNIKGINFDLPHVVQDAPSYPGTF
>CCG016104.1
MADETSAARNDARLAILELANMISVPMSLNAVVHLNIPNAIWQHGANSPLTASQILSLLPPSSSSAPPDPENLQRILRMLTSYNVFNEHVDSLTSSRSYSLTEVGQTLITDSDGLSYGAYVLQHHQDALMKAWPLVHEAVLDPTTEPFVKANGEAAYDYYGKKPEMNELMLKAMSGVSVPFMKAILDGYDGFEGVRTLVDVGGSAGDCLKMILRKYPGVVEGINFDLPEVVAKPPPIAGVRHVGGDMFKSIPSGDAIFMKWVLTTWTDDECRLIMENCYKALPTGGKLIACEPVLPEESDDSHRTRALLEGDIFVMTIYRAKGKHRTEDEFKKLGILAGFPQFRAFYIDHFYTVLEFQK
>CCG029380.1
MFESVPSADAIFMKWILHDWSDAHCLKLLKNCYKALPDNGIVIVVDANLPDVPDNSSTTKSISQMDVLMLAQNPGGKERRAHELLALAKEAGFSGIRHVGCYCLFWVMEFYKCNI
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University