Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Raphanus sativus
Species name
Radish (Raphanus sativus) is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Raphanus_sativus.FLS.fa
Download
>rna-XM_018612851.1
MSEMRTKNQVEDSIIQESLLTCIDLGNSDVHQSALLLKKACLDSGFFYVINHGISKKVMEKAFEQSQKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQIQGDHKEGYYIGSEVPRDDPQWDRPFYGPNTWPNPDVLPGWRETMEEYHQEALRVCKAIAKLLALSLNLDADYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMITLLATDGVMGLQICKDRNAKIQKWEYVPSIKGAIVVNLGDMMERWSNGLFRSTLHRVILNGQTRYSIPFFVEPNHDCIIECLPTCQSENNLPKYSAIKCSAYLTQRYEESHLKFSSYKKQT
>rna-XM_018622780.1
MELPVVDLSRYLQSSSNGDDLLDSCREVSRILKETGALLVKDPRCSAQDNDRFIDMMETYFSKPDEFKRLQQRPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPEEYKPHVPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPEGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKDAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFAGSSLASNYPAIPAGEYVEQELSVINLKGKDALS
>rna-XM_018630657.1
MENHTKERLQDPTMQVPSLNCIDLADHDLHRSVVSLKQACLDSGFFYVINHGINEEFMEDVFEQSKKFFALPLEEKMKVLRNEKHRGYTPVLDEILDPENQVNGDHKEGYYIGIEVPKDDPDYDKPFYGPNPWPDSDVLPQWRETMEKYHQEALRVSKDIGRLLALALDLDANYFDTPEMLGKPIATMRLLHYQGVSDPSQGIYACGAHSDYGMMTLLATDGVMGLQICKDKNAKPQKWEYVPAIKGAYIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHECLVECLPTCKSESNVPKYPAIKCSAYLTQRYKETHAELSIYNQEI
>rna-XM_018630114.1
MEAERDEHTSPPSERIPIVDLSDPEDELVVRTVVKASEEWGAFQVVNHGIPAELIRRLQEVGKQFFELTAAEKEAVGKLPGSKSVQGYDAKNPKRRVSWSEHILHKIWPQSCIDYRFWPKNPPNYREVNEEYARHLTKLSETIMGCLSKGLGLGREALKEGLGGAEYMLRINYYPPSSDLVVGAEAHSDLAAMALLVSNGVPGLQVFKDNHWFDVEHIDSAVVVLVGDQIMRMSNGRYKNVLHRAIMYKEKTRMTWPVLVDPKGGLAVGPLPELIGEDNPPKFEPLTFEDYVYRKFDMLLRDG
>rna-XM_018633748.1
MSECMKRMEMARNQHISPSPLPLEKAKPAITYQGIATDVPIVDLSNPDEDLVASAVVKASEEWGIFQVVNHGIPQELIRRLKEVGTEFFELPETEKEAVAKPEDSMDVEGYRTKYQKDLEGRNAWVDHLFHRISPPSRVSYRFWPKLPMEYREVNEDYARHLKKLSEKIMEWLSEGLGLRRGALKEGLGGETVEYLMKINYYPPCPDQDLVVGAPDHTDVNGITFLVANEALGLQAFKDNHWVDVKCTTSGIIVIVGDQFHRMSNGKYMSAKHRAKMDKEKTRISWPVFVESSLDHEFAPLRELITGEDNAPKFKPYVYKDFKLRKLKKLPLD
>rna-XM_018630112.1
MEMERNQHISPPSLLAAPEEIPVVDLSNPNEDLVARAVVKASKEWGIFHVVNHGIPQELIRQLLEICWAGRYFYELPETEKQAVAKPEDDSTDDIEGYNSNFKGRARADHLFHRIWPPSRINYRFWPKNSPYYREVNEEYAKHIKKLSEKIMEWLSEGLGLHRQAFKECLGGEKVEYLMKLIFYPPCPNPDSNDGAPPHTDFNGITFLIANEVHGLQAFKDNHWIDVKYDDSIILVIIADLFHRMSNGKYKSAEHRATMDTEKTRISWPVFVEPNLDQVVGPLPELLHGLDTAPKFKPYAYKDIKFLLRYGLPLD
>rna-XM_018635083.1
MVVQRDQHTSPPSEKIPIIDLSNPDEELVCHAVVKASQEWGIFHVVNHGVSMDLIRRLKEVGTQFFELPETEKKDVAKPDTSQDFEGYTRNLKYTEGEVWADNLFHRIWPPSCINYNYWPKNPPHYREVIEEYTKETKKLSERLLGILSEGLGLRHEALKEGLGGEKTEYVLRINNFPPNPQPGATLGLPEHTDIIAMALIVTNEVPGLQVFNNDHWFDVQYVPSSITVTIGDQILRLSNGKYKNVLHRVTVDKEKQRMSWPVFVDDTVIRPLPEIITGDNPSVFKPIICKDFKYRRLFKLPVD
>rna-XM_018584191.1
MVAEREQDILSSRIPIIDVSNPDQGLVARAVVKASEEWGVFQLVNHGIPTELIQRLQNVGRRFFELSETEKKAVAKPDNSREGYARRYTLDLEKKIGTVDQLYHNIWPPSVVNHSYWPKNPQDYREVNEEYTRQVKILSEKIMEWLSEGLGLRREAINEVVGGEYLLNVNYYPPCPHPDLIEGLDAHTDVSGLTLLLTNEIPGLQVFKDDQWIEVEYIPFAVIVNISDQILRVSNGKYKSVLHKTTVDKERTRMSWAVLVRPTSDMVVGPFPELIGDDPPKFKSMLYKDYIYRKVRNLPFVDLDS
>rna-XM_018578189.1
MAESVPSKFQETSPVDIPIVDLSNPNEDLVARAVVKASETWGMFQVVNHGIPIELMRRLKELGTEFFELPEKEKEAVARPADSTDLEGYTTDYKKDGEGRKTWADHLFHRVWPPSRINYRFWPKNSPDYREVNEEYAREIKKLSEKIMGWLSEGLGLHRDALKEGLGGEKVEYLMKIIFYPPCPKLELLYGAPHHTDLNGITFLIADEVDGLQAFQDNKWVDVKYDDSGIVVIIADQIKRMSNGRYKSGEHRATMDTVRTRLSWPVFAEPNLDHVVGPLAELVIDDAPKYKPYVYREYKFLKMNKFPLD
>rna-XM_018586825.1
MEFERVQDISSSSLRTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEETVARAVVKASEEWGIFQVVNHGIPAELIKRLQEVGRTFFELPSSEKESVAKPVDAKDIEGYGTKLQKEVEGKKAWVDHLFHRIWPPSCVNYSFWPNNPPEYREVNEEYALHVKKLSETLLGILSEGLGLRREALREGLGGDLAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKDRTRMSWPVFLEPHREMVVGPLPELISDGNPPKYKPFAFKDYSYRKLNKLPLD
>rna-XM_018598711.1
MEVERDQTESSPTIPIVDLSNLDKELVARAVVKASQEWGVFQVVNHEIPKELIQSLKKVGTHFFELSSEAEKESVATPENSKDIQGYVTNIQKDVEDKVDRVDTLFHNLSPPSWVDYRYWPKDPPEYRQVNEEYAVYAKKLAEKILGWLSEGLGLEREALFNKGFGGGDKAVYTMKINYYPPCRQQANLVLGLHPHTDIHGLTILVP
>rna-XM_018611865.1
MEVERVGQYASPPSETVPIIDLSDPDDELVERALVKASEEWGAFQVVNHEIPAEIMRRFQDVGRKFFELPAPEKEAVAMVPGSKSIEGYDAKNLKLAKSWDDHLLHKIWPQSCINYRFWPKNPPDYRFQRLQASCSRMQPGTPLLLQVALAGFAQTRPAHTCFKDQRTDVI
MSEMRTKNQVEDSIIQESLLTCIDLGNSDVHQSALLLKKACLDSGFFYVINHGISKKVMEKAFEQSQKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQIQGDHKEGYYIGSEVPRDDPQWDRPFYGPNTWPNPDVLPGWRETMEEYHQEALRVCKAIAKLLALSLNLDADYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMITLLATDGVMGLQICKDRNAKIQKWEYVPSIKGAIVVNLGDMMERWSNGLFRSTLHRVILNGQTRYSIPFFVEPNHDCIIECLPTCQSENNLPKYSAIKCSAYLTQRYEESHLKFSSYKKQT
>rna-XM_018622780.1
MELPVVDLSRYLQSSSNGDDLLDSCREVSRILKETGALLVKDPRCSAQDNDRFIDMMETYFSKPDEFKRLQQRPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPEEYKPHVPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPEGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKDAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFAGSSLASNYPAIPAGEYVEQELSVINLKGKDALS
>rna-XM_018630657.1
MENHTKERLQDPTMQVPSLNCIDLADHDLHRSVVSLKQACLDSGFFYVINHGINEEFMEDVFEQSKKFFALPLEEKMKVLRNEKHRGYTPVLDEILDPENQVNGDHKEGYYIGIEVPKDDPDYDKPFYGPNPWPDSDVLPQWRETMEKYHQEALRVSKDIGRLLALALDLDANYFDTPEMLGKPIATMRLLHYQGVSDPSQGIYACGAHSDYGMMTLLATDGVMGLQICKDKNAKPQKWEYVPAIKGAYIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHECLVECLPTCKSESNVPKYPAIKCSAYLTQRYKETHAELSIYNQEI
>rna-XM_018630114.1
MEAERDEHTSPPSERIPIVDLSDPEDELVVRTVVKASEEWGAFQVVNHGIPAELIRRLQEVGKQFFELTAAEKEAVGKLPGSKSVQGYDAKNPKRRVSWSEHILHKIWPQSCIDYRFWPKNPPNYREVNEEYARHLTKLSETIMGCLSKGLGLGREALKEGLGGAEYMLRINYYPPSSDLVVGAEAHSDLAAMALLVSNGVPGLQVFKDNHWFDVEHIDSAVVVLVGDQIMRMSNGRYKNVLHRAIMYKEKTRMTWPVLVDPKGGLAVGPLPELIGEDNPPKFEPLTFEDYVYRKFDMLLRDG
>rna-XM_018633748.1
MSECMKRMEMARNQHISPSPLPLEKAKPAITYQGIATDVPIVDLSNPDEDLVASAVVKASEEWGIFQVVNHGIPQELIRRLKEVGTEFFELPETEKEAVAKPEDSMDVEGYRTKYQKDLEGRNAWVDHLFHRISPPSRVSYRFWPKLPMEYREVNEDYARHLKKLSEKIMEWLSEGLGLRRGALKEGLGGETVEYLMKINYYPPCPDQDLVVGAPDHTDVNGITFLVANEALGLQAFKDNHWVDVKCTTSGIIVIVGDQFHRMSNGKYMSAKHRAKMDKEKTRISWPVFVESSLDHEFAPLRELITGEDNAPKFKPYVYKDFKLRKLKKLPLD
>rna-XM_018630112.1
MEMERNQHISPPSLLAAPEEIPVVDLSNPNEDLVARAVVKASKEWGIFHVVNHGIPQELIRQLLEICWAGRYFYELPETEKQAVAKPEDDSTDDIEGYNSNFKGRARADHLFHRIWPPSRINYRFWPKNSPYYREVNEEYAKHIKKLSEKIMEWLSEGLGLHRQAFKECLGGEKVEYLMKLIFYPPCPNPDSNDGAPPHTDFNGITFLIANEVHGLQAFKDNHWIDVKYDDSIILVIIADLFHRMSNGKYKSAEHRATMDTEKTRISWPVFVEPNLDQVVGPLPELLHGLDTAPKFKPYAYKDIKFLLRYGLPLD
>rna-XM_018635083.1
MVVQRDQHTSPPSEKIPIIDLSNPDEELVCHAVVKASQEWGIFHVVNHGVSMDLIRRLKEVGTQFFELPETEKKDVAKPDTSQDFEGYTRNLKYTEGEVWADNLFHRIWPPSCINYNYWPKNPPHYREVIEEYTKETKKLSERLLGILSEGLGLRHEALKEGLGGEKTEYVLRINNFPPNPQPGATLGLPEHTDIIAMALIVTNEVPGLQVFNNDHWFDVQYVPSSITVTIGDQILRLSNGKYKNVLHRVTVDKEKQRMSWPVFVDDTVIRPLPEIITGDNPSVFKPIICKDFKYRRLFKLPVD
>rna-XM_018584191.1
MVAEREQDILSSRIPIIDVSNPDQGLVARAVVKASEEWGVFQLVNHGIPTELIQRLQNVGRRFFELSETEKKAVAKPDNSREGYARRYTLDLEKKIGTVDQLYHNIWPPSVVNHSYWPKNPQDYREVNEEYTRQVKILSEKIMEWLSEGLGLRREAINEVVGGEYLLNVNYYPPCPHPDLIEGLDAHTDVSGLTLLLTNEIPGLQVFKDDQWIEVEYIPFAVIVNISDQILRVSNGKYKSVLHKTTVDKERTRMSWAVLVRPTSDMVVGPFPELIGDDPPKFKSMLYKDYIYRKVRNLPFVDLDS
>rna-XM_018578189.1
MAESVPSKFQETSPVDIPIVDLSNPNEDLVARAVVKASETWGMFQVVNHGIPIELMRRLKELGTEFFELPEKEKEAVARPADSTDLEGYTTDYKKDGEGRKTWADHLFHRVWPPSRINYRFWPKNSPDYREVNEEYAREIKKLSEKIMGWLSEGLGLHRDALKEGLGGEKVEYLMKIIFYPPCPKLELLYGAPHHTDLNGITFLIADEVDGLQAFQDNKWVDVKYDDSGIVVIIADQIKRMSNGRYKSGEHRATMDTVRTRLSWPVFAEPNLDHVVGPLAELVIDDAPKYKPYVYREYKFLKMNKFPLD
>rna-XM_018586825.1
MEFERVQDISSSSLRTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEETVARAVVKASEEWGIFQVVNHGIPAELIKRLQEVGRTFFELPSSEKESVAKPVDAKDIEGYGTKLQKEVEGKKAWVDHLFHRIWPPSCVNYSFWPNNPPEYREVNEEYALHVKKLSETLLGILSEGLGLRREALREGLGGDLAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKDRTRMSWPVFLEPHREMVVGPLPELISDGNPPKYKPFAFKDYSYRKLNKLPLD
>rna-XM_018598711.1
MEVERDQTESSPTIPIVDLSNLDKELVARAVVKASQEWGVFQVVNHEIPKELIQSLKKVGTHFFELSSEAEKESVATPENSKDIQGYVTNIQKDVEDKVDRVDTLFHNLSPPSWVDYRYWPKDPPEYRQVNEEYAVYAKKLAEKILGWLSEGLGLEREALFNKGFGGGDKAVYTMKINYYPPCRQQANLVLGLHPHTDIHGLTILVP
>rna-XM_018611865.1
MEVERVGQYASPPSETVPIIDLSDPDDELVERALVKASEEWGAFQVVNHEIPAEIMRRFQDVGRKFFELPAPEKEAVAMVPGSKSIEGYDAKNLKLAKSWDDHLLHKIWPQSCINYRFWPKNPPDYRFQRLQASCSRMQPGTPLLLQVALAGFAQTRPAHTCFKDQRTDVI
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University