Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Raphanus sativus
Species name
Radish (Raphanus sativus) is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Raphanus_sativus.4CL.fa
Download
>rna-XM_018577182.1
MAFRQQEALSLTKKTDQDPSQDFIFRSKLPDISIPNHLPLTEYVFSGHRDGDSTTKCLIDGATGRVFTYADVQITLRRIAAGIYGMGIRHGDTVMLLLPNSPEFALSFLAVVHLGAVSTSANPLFTQTEIAKQAKASAVKMIITKSCHVHKLTNLQQLGVVIVCVDDGNDVVSLADGCVSFRELTQADETKLPKPQISPEDTVSIPYSSGTTGLPKGVMITHKGLVTSISQKVDGENPNLNFTRDDVIICFLPMFHTFTHSSLMLSAMRTGAAFLILPRFELNLVMELIQRYKVTVVPVAPPVVLAFVKSLETEKYDLSSVRMMLSGAATLKKELEDAVRLKLPNAIFGQSYGMTEAGTVANSLAFAKSPFKTKSGSCGTVIRNAEMKVVDTISGVSLPRNKPGEICIRGDQLMKGYLNDPIATVRTIDKDGWLHTGDIGFVDEDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPCIKDAAVVAMKDEIAGEVPVACVVKSEGSKLTEEDVKNYVNKQVVHYKRIKMVLFVKSIPKSASGKLLRKVLRAKL
>rna-XM_018636592.1
MVLQQQQQTLFVTKKTDQEPSHEFIFRSKLPDISIPNHLPLTDYIFQKFSGDGGGDSTATCLIDGATGRIFTFGDVQISLRRIAAGIHGLGICQRDTVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTESEIEKQAKASATKMIITKPCYVDKLTNLKNDGVLIVCVEDENDAVSVADGCVSFKELTQADETEFPKPEISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTGDDVILCFLPMFHIYALDALMLSAMRTGAAILIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFVKSPETERYDLSSVRMMLSGAATLKKELEDAVRLKFPSAIFGQGYGMTESGTVAKSLAFAKNPFKTKSGACGTVIRNAEMKVVDTITGVSLPYNKPGEICIRGDQLMKGYLNDPEATAITIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPYIDDAAVVAMKDEIADEVPVAYVVRSEGSQLTEDDVKSYVNKQVVHYKRIKMVFFTEAIPKAVSGKILRKELRAKLEAEYRK
>rna-XM_018633772.1
MALRQEETLSLARKTTEQEPSQDIIFRSKLADISIPNHLPLIDYIFQRFSGGGDDGGSTTTCLIDGVTGRIFTYADVQITLRRISAGLHRHGIRHGDTVMLLLPNSPEFALSCLALAYLGAISTPANPLFTPPEIAKQAKASATKMIITKPCYVDKLTNLENVLIVCVDEYDIPLPEGCLSFTELTQADENELPKPEISPEDTVLMPFSSGTTGHPKGVMITHKGVVTSTAQKVDGENPHLNFTRDDVIICFLPMFHTYTHNSLMLSAMRAGAALLILPRFELNMVMEMIEKYKVTVVPMAPPVVLALVNSQETDRYDLSSVRMMFSGSGALTKELEDAVSLKLPSAIFGQSYGMTEMGTVAKSLALAKNPFKIKSGSCGTVIRNAEMKVVDIITGVSLPRNKHGEICIRGDQLMKGYLNDLEATAQTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHHFIEDVAVVA
>rna-XM_018636487.1
MAPQEDTMQKQSSNNSDVIFRSKLPDIYIPNHLPLHEYIFQSISEFASKPCLINGPTGRVYTYSDVHVASRRIAAGFQKLGVERNDVVMILLSNCPEFVLSFLAASFRGATATGNPFFTPAEIAKQAKASNSKLIVTESRYVDKIKDLQNDGVVVVCTDEEPSPIPEGCLRFTELTQSTEEIDAVEISSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALNSIMLCGLRVGASILIMPKFEINLLLELMQRCKVTVAPMVPPVVLAIAKSQETEKYDLSSVRVMKSGAAPLGKEIEDAVSAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIIDPDTGDSLSKNKPGEICIRGQQIMKGYLNNPEATAETIDKEGWLHTGDIGLIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLIGHQDITDVAVVGMKEEAAGEVPVAFVVKSKDSELSEDEVKQFVAKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLANGLVN
>rna-XM_018636594.1
MVLQQQQQTLFVTKKTDQEPSHEFIFRSKLPDISIPNHLPLTDYIFQKFSGHGGGDSTATCLIDGATGRIFTYGNVQISSRRIAAGIHGLGIRQRDTVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTEPEIEKQAKASATKMIITKPCYVDKLTNLKNDGVLIVCVEDENDAVPVADGCVSFKELTQSDETELPKPEISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTGDDVILCFLPMFHIYALDALMLSAMRTGAAILIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFVKSPETERYDLSSVRMMLSGAAMLKKELEDAVRLKFPSAIFGQVSFS
>rna-XM_018590675.1
MITATLQEPQIHQPVDTTTSPTDAPPSPPPRIFRSKLPDIDIPNHLPLHTYCFEKLPSVSDKPCLIVGSTGKSYTYGETHLICRRVAAGLHKTGIRKGDVIMVLLQNSAEFVFTFMGASMIGAVSTTANPFYTSQEIHKQVKSSGAKLIVTHSHYVDKLRNLDEETRIGEDLTVITTEVPPPENCLPFSALLLAKEEANSLDVAVDVGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLKSNDVVLCVLPLFHIYSLNSVLLNSIRSGATVLLMHKFEIGALLDLIQRHKVTVAALVPPLVIALAKNPTVNSYDLSSVRLVLSGAAPLGKDLEDSLRSRLPQAVLGQGYGMTEAGPVLSMSLGFAKEPIPSKSGSCGTVVRNAELKVVHLETRLSLGYNQTGEICIRGQQIMKEYLKDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELEALLISHHSIADAAVVPQRDEVAGEVPVAFVVRSNGNVITEEDIKEYISKQVVFYKRLHKVFFVPSIPKSPSGKILRKDLKAKLC
>rna-XM_018602022.1
MEKSGYGRDGIYRSLRPPLLLPKDPNTSLVSFLFRNSSSYPSKPAIIDSDTNHSLTFSQLKSSVSRLAHGFLRLGIRQNDVVLIFAPNSHHFPLCFLAVSAVGAVFTTANPLYTTAELSKQIKDSNPKLIISVDHLFDKVKDFNLPVVLLGSSSSSSPKTLTFENVMDLSDPGSNLPVVEIKQSDTAALLYSSGTTGISKGVELTHRNFIAAALMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVIAYSQLQRGSALVSMARFELELVLRNIERYRVTQLWVVPPVFLALAKQSVVKKYDLSSLKYIGSGAAPLGKDLMEECGKNIPNVVLMQGYGMTETCGIVSVEDPRLGKRNSGSAGMLAPGIEAQIVSVESGKPQPPNQQGEIWVRGPNMMKGYINNPQATKETIDKKGWVHTGDLGYFNEDGNLFVVDRLKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPLPDEEAGEVPIAFVVRSPNSSITEEDIQKFIAKQVAPYKRLRRVSFISTVPKSAAGKILRRELVQQVRSKM
>rna-XM_018598341.1
MLSPGIEAQIVSVESGKPQPPNQQGEIWVRGPNMMKGYFNNPQATREKIDKKGWVHTGDLGYFNEDGNLFVVDRLKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPLPDEEAGEVPIAFVVRSPSSSITEEDIQKFIAKQVAPYKRLRRVSFISSVPKSAAGKILRRELVSASKIQDLKHLMVSSSSRIPKTVEQSMDVDAQVEGVENQRTQDRGKAIAVDATSGVSSQSKKVMKVVVPRSGVWKHFTRTKEDQDRCICHYCQKIFSCAAKSGTSNLQKHLSICKEYQAWITSQGTSKMEINTDGNLKSSKVVEAVFREASNELI
>rna-XM_018599313.1
MEAQKQDNEHIFRSLYPSVPIPDKLTLPEFVLQGVEEFTEKVAFVEAVTGKAVTYGDVVRDTKRLAKALTSVGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGARGIITDSTNFEKVKNLGLPVILLGEEKIEGAVNWKDILEAGDKCGDNNRVEILQTDLCALPFSSGTTGLQKGVMLTHRNLIANLCSTLFSVRSEMIGEIVTLGLIPFFHIYGIVGICCATMKNKGKVVAMSRYDLRLFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLRSVMTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPDKGQGIAKRNSVGFILPNLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFENKEETEKTIDEEGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQ
>rna-XM_018599241.1
MENSGYGRDGIYRSLRPTLVLPEDPNTSLVSFLFSNTSSYPTKPALVDSDTGDSMSFSQLKAAVSRLAHGFLRLGIRKNDVVMIFAPNSHQFPLCFLAVTAIGGVFTTVNPSYTVDEVSKQVKDCNPKLIISVQELLDKVKTFDLPIVLLGQSHDSSSKILMSLNNVMELSEPVSDLPHVDVKQSDTAALLYSSGTTGMSKGVELTHGNFIATALMVTMDQDLMGEYHGVFLCFLPMFHVFGLSVVTYAQLQRGNAVVSMAKFDLERLLKNIEKYRVTQLWVVPPVFLALAKQSIVKKYDLSSLKYIGSGAAPLGKDSMEECARNIPNVVLMQGYAMTETCGIVSMEDPRRGKRNSGSAGMLASGIEAQVVSVESGKPQPPNQQGEIWVRGRNMMKGYLNNPEATKETIDENGWVHTGDLGYFNEDGNLFIVERLKELIKCNGFQVAPAELEGLLVSHPELLDAIVIPFPDVYAGEVPIAFVVRSPNTSITEEDVQKFIAKQVAPYKRLRRVSFVPSVPKSLAGKLLRRELREQVRSKM
MAFRQQEALSLTKKTDQDPSQDFIFRSKLPDISIPNHLPLTEYVFSGHRDGDSTTKCLIDGATGRVFTYADVQITLRRIAAGIYGMGIRHGDTVMLLLPNSPEFALSFLAVVHLGAVSTSANPLFTQTEIAKQAKASAVKMIITKSCHVHKLTNLQQLGVVIVCVDDGNDVVSLADGCVSFRELTQADETKLPKPQISPEDTVSIPYSSGTTGLPKGVMITHKGLVTSISQKVDGENPNLNFTRDDVIICFLPMFHTFTHSSLMLSAMRTGAAFLILPRFELNLVMELIQRYKVTVVPVAPPVVLAFVKSLETEKYDLSSVRMMLSGAATLKKELEDAVRLKLPNAIFGQSYGMTEAGTVANSLAFAKSPFKTKSGSCGTVIRNAEMKVVDTISGVSLPRNKPGEICIRGDQLMKGYLNDPIATVRTIDKDGWLHTGDIGFVDEDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPCIKDAAVVAMKDEIAGEVPVACVVKSEGSKLTEEDVKNYVNKQVVHYKRIKMVLFVKSIPKSASGKLLRKVLRAKL
>rna-XM_018636592.1
MVLQQQQQTLFVTKKTDQEPSHEFIFRSKLPDISIPNHLPLTDYIFQKFSGDGGGDSTATCLIDGATGRIFTFGDVQISLRRIAAGIHGLGICQRDTVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTESEIEKQAKASATKMIITKPCYVDKLTNLKNDGVLIVCVEDENDAVSVADGCVSFKELTQADETEFPKPEISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTGDDVILCFLPMFHIYALDALMLSAMRTGAAILIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFVKSPETERYDLSSVRMMLSGAATLKKELEDAVRLKFPSAIFGQGYGMTESGTVAKSLAFAKNPFKTKSGACGTVIRNAEMKVVDTITGVSLPYNKPGEICIRGDQLMKGYLNDPEATAITIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPYIDDAAVVAMKDEIADEVPVAYVVRSEGSQLTEDDVKSYVNKQVVHYKRIKMVFFTEAIPKAVSGKILRKELRAKLEAEYRK
>rna-XM_018633772.1
MALRQEETLSLARKTTEQEPSQDIIFRSKLADISIPNHLPLIDYIFQRFSGGGDDGGSTTTCLIDGVTGRIFTYADVQITLRRISAGLHRHGIRHGDTVMLLLPNSPEFALSCLALAYLGAISTPANPLFTPPEIAKQAKASATKMIITKPCYVDKLTNLENVLIVCVDEYDIPLPEGCLSFTELTQADENELPKPEISPEDTVLMPFSSGTTGHPKGVMITHKGVVTSTAQKVDGENPHLNFTRDDVIICFLPMFHTYTHNSLMLSAMRAGAALLILPRFELNMVMEMIEKYKVTVVPMAPPVVLALVNSQETDRYDLSSVRMMFSGSGALTKELEDAVSLKLPSAIFGQSYGMTEMGTVAKSLALAKNPFKIKSGSCGTVIRNAEMKVVDIITGVSLPRNKHGEICIRGDQLMKGYLNDLEATAQTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHHFIEDVAVVA
>rna-XM_018636487.1
MAPQEDTMQKQSSNNSDVIFRSKLPDIYIPNHLPLHEYIFQSISEFASKPCLINGPTGRVYTYSDVHVASRRIAAGFQKLGVERNDVVMILLSNCPEFVLSFLAASFRGATATGNPFFTPAEIAKQAKASNSKLIVTESRYVDKIKDLQNDGVVVVCTDEEPSPIPEGCLRFTELTQSTEEIDAVEISSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALNSIMLCGLRVGASILIMPKFEINLLLELMQRCKVTVAPMVPPVVLAIAKSQETEKYDLSSVRVMKSGAAPLGKEIEDAVSAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIIDPDTGDSLSKNKPGEICIRGQQIMKGYLNNPEATAETIDKEGWLHTGDIGLIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLIGHQDITDVAVVGMKEEAAGEVPVAFVVKSKDSELSEDEVKQFVAKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLANGLVN
>rna-XM_018636594.1
MVLQQQQQTLFVTKKTDQEPSHEFIFRSKLPDISIPNHLPLTDYIFQKFSGHGGGDSTATCLIDGATGRIFTYGNVQISSRRIAAGIHGLGIRQRDTVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTEPEIEKQAKASATKMIITKPCYVDKLTNLKNDGVLIVCVEDENDAVPVADGCVSFKELTQSDETELPKPEISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTGDDVILCFLPMFHIYALDALMLSAMRTGAAILIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFVKSPETERYDLSSVRMMLSGAAMLKKELEDAVRLKFPSAIFGQVSFS
>rna-XM_018590675.1
MITATLQEPQIHQPVDTTTSPTDAPPSPPPRIFRSKLPDIDIPNHLPLHTYCFEKLPSVSDKPCLIVGSTGKSYTYGETHLICRRVAAGLHKTGIRKGDVIMVLLQNSAEFVFTFMGASMIGAVSTTANPFYTSQEIHKQVKSSGAKLIVTHSHYVDKLRNLDEETRIGEDLTVITTEVPPPENCLPFSALLLAKEEANSLDVAVDVGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLKSNDVVLCVLPLFHIYSLNSVLLNSIRSGATVLLMHKFEIGALLDLIQRHKVTVAALVPPLVIALAKNPTVNSYDLSSVRLVLSGAAPLGKDLEDSLRSRLPQAVLGQGYGMTEAGPVLSMSLGFAKEPIPSKSGSCGTVVRNAELKVVHLETRLSLGYNQTGEICIRGQQIMKEYLKDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELEALLISHHSIADAAVVPQRDEVAGEVPVAFVVRSNGNVITEEDIKEYISKQVVFYKRLHKVFFVPSIPKSPSGKILRKDLKAKLC
>rna-XM_018602022.1
MEKSGYGRDGIYRSLRPPLLLPKDPNTSLVSFLFRNSSSYPSKPAIIDSDTNHSLTFSQLKSSVSRLAHGFLRLGIRQNDVVLIFAPNSHHFPLCFLAVSAVGAVFTTANPLYTTAELSKQIKDSNPKLIISVDHLFDKVKDFNLPVVLLGSSSSSSPKTLTFENVMDLSDPGSNLPVVEIKQSDTAALLYSSGTTGISKGVELTHRNFIAAALMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVIAYSQLQRGSALVSMARFELELVLRNIERYRVTQLWVVPPVFLALAKQSVVKKYDLSSLKYIGSGAAPLGKDLMEECGKNIPNVVLMQGYGMTETCGIVSVEDPRLGKRNSGSAGMLAPGIEAQIVSVESGKPQPPNQQGEIWVRGPNMMKGYINNPQATKETIDKKGWVHTGDLGYFNEDGNLFVVDRLKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPLPDEEAGEVPIAFVVRSPNSSITEEDIQKFIAKQVAPYKRLRRVSFISTVPKSAAGKILRRELVQQVRSKM
>rna-XM_018598341.1
MLSPGIEAQIVSVESGKPQPPNQQGEIWVRGPNMMKGYFNNPQATREKIDKKGWVHTGDLGYFNEDGNLFVVDRLKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPLPDEEAGEVPIAFVVRSPSSSITEEDIQKFIAKQVAPYKRLRRVSFISSVPKSAAGKILRRELVSASKIQDLKHLMVSSSSRIPKTVEQSMDVDAQVEGVENQRTQDRGKAIAVDATSGVSSQSKKVMKVVVPRSGVWKHFTRTKEDQDRCICHYCQKIFSCAAKSGTSNLQKHLSICKEYQAWITSQGTSKMEINTDGNLKSSKVVEAVFREASNELI
>rna-XM_018599313.1
MEAQKQDNEHIFRSLYPSVPIPDKLTLPEFVLQGVEEFTEKVAFVEAVTGKAVTYGDVVRDTKRLAKALTSVGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGARGIITDSTNFEKVKNLGLPVILLGEEKIEGAVNWKDILEAGDKCGDNNRVEILQTDLCALPFSSGTTGLQKGVMLTHRNLIANLCSTLFSVRSEMIGEIVTLGLIPFFHIYGIVGICCATMKNKGKVVAMSRYDLRLFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLRSVMTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPDKGQGIAKRNSVGFILPNLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFENKEETEKTIDEEGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQ
>rna-XM_018599241.1
MENSGYGRDGIYRSLRPTLVLPEDPNTSLVSFLFSNTSSYPTKPALVDSDTGDSMSFSQLKAAVSRLAHGFLRLGIRKNDVVMIFAPNSHQFPLCFLAVTAIGGVFTTVNPSYTVDEVSKQVKDCNPKLIISVQELLDKVKTFDLPIVLLGQSHDSSSKILMSLNNVMELSEPVSDLPHVDVKQSDTAALLYSSGTTGMSKGVELTHGNFIATALMVTMDQDLMGEYHGVFLCFLPMFHVFGLSVVTYAQLQRGNAVVSMAKFDLERLLKNIEKYRVTQLWVVPPVFLALAKQSIVKKYDLSSLKYIGSGAAPLGKDSMEECARNIPNVVLMQGYAMTETCGIVSMEDPRRGKRNSGSAGMLASGIEAQVVSVESGKPQPPNQQGEIWVRGRNMMKGYLNNPEATKETIDENGWVHTGDLGYFNEDGNLFIVERLKELIKCNGFQVAPAELEGLLVSHPELLDAIVIPFPDVYAGEVPIAFVVRSPNTSITEEDVQKFIAKQVAPYKRLRRVSFVPSVPKSLAGKLLRRELREQVRSKM
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University