Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Pyrus betulifolia
Species name
Pyrus betulifolia is a species of eudicot in the family Rosaceae (rose family).
Species info
Gene sequence info
Pyrus_betulifolia.ROMT.fa
Download
>Chr1.g58127.m1
MSSSNAVGATSHELLGAQAQLWNHIFQFINSMSLKCAVQLGIADVIHNHGQPISLSELIAGLNVHPSKAHFVSRLMLILVHSNFFAQHHHVHHDRADVEEEEAVVLYSLTPSSRLLLKDGPLSTTPFLLAILDPVVTTPFHLMGAWLKINGGDDPAATCTPFEMENGMPFWELGAQEPRFGNLFNEAMEADSKLIGRVVVEECGGVFEGLKSLMDVGGGSGTMAKAIANAFPNINCTVFDQPHVVGGLQGTTHNLGFMGGDMFEEIPPANAILLKWIMHDWNDEESVTILKKCREAISLSKNEGGNKKIIIIDIVVGYVDNKKKMMDKKSIETQLMFDMLMMSIVHGKERSKLEWEKIFFAAGFTHYNITHTLGLRSLIEVYP
>Chr1.g58176.m1
MALSNREATSHELLGAQAHVWNHIFQFINSMSLKCAVQLGIPDVIHNHGQPISLSELIAGLNVHPSKAHFISRLMRILVHSNFFAQHHHVHHNHDNVEEEEAVELYSLTPASRLFLKDGPLNTTPFLLMILDPVMTDPFHSMGAWLQMNGGDDLVPRTPFEVANGMPFWELSAKKPRFGDSFNEAMEADSKLIARAVVEECGGVFEGLKSLVDVGGGTGTMAKAIANAFPNINCTVFDQPHVVANLEGTTHNLGFVGGDMFDKIPPANAILLKWILHDWNDEESVKILKKCREAMLVSKNEGRKKIIIIDMVVGYVNNKMQLDKKSIETQLMFDMLMMSTVTSNERSELEWEKIFLAAGFTHYNITHTLGLRSLIEVYL
>Chr1.g58124.m1
MGLSNGEVGATSHELLRAQGRIWNHMFQFTNSMALKCAAQLGIPDVIHSHGQPISLSELIAALNVHPSRAHFVSRLMRILVHSDFFAQHHHVHHDCDDVEDEEAVVLYSLTPTSRLLLKDGPSNTTPLILMILDPVLTTPFHLMGAWLQMNGGDGPATICTPFEMENGMPFWDLAAQEPRFGNLFDEAMEADSKRLGREVVEECGGVFEGLKSLVDVGGGTGTMAKAIANSFPSINCTVFDQPHVVADLQGITHNLGFVGGDMFVEIPPANAILLKWILHDWSDEESVKILKKCREAILLSKNDEGNKKIIIIDIVVGHVDNKEKMVDKKSIETQLMFDMLMMSIVTGKERSESEWKKIFLACWFHSL
>Chr1.g58121.m1
MGLSNGEVGATSHELLGAQAQIWNHMFQFINSMALKCAAQLGIPDVIHNHGQPISLSDLITALNVHPSRAHFVSRLMRILVHSNFFAQHHHVHHDCDDVEEEEAVVLYSLTPTSRLLLKDGPSNTTPLILMILDPVLTTPFQLMGAWLQMNGGDDPATICTPFEMENGMPFWDLAAQEPRFGNLFDEAMEADSKLLGREVVEECGGVFEGLKSLVDVGGGTGAMAKAIANAFPSINCIVFDQPHVVADLQGTTHNLGFVGGDMFVEIPPANAILLKWILHDWSDEESVKILKKCREAILLSKNDEGNKKIIIIDIVVGHVDNKEKMVDKKSIETQLMFDMLMMSAFTGKERSESEWKKIFLAASFTHYNITHTFGFRSLIELYP
>Chr1.g58171.m1
MGLSNGEVGAISHEDLGAQAHVWNHMFQFINSMSLKCAVQLGIPDVIHNHGQPISLSELIAGLNVHPSKAHFISRLMRILVHSDFFAQHHHVHHDRDDAEEEVAVVLYSLTPASGLFLKDGPLNTTPFLLMILDPVITDPFHSMSNWLHMNCGDDLVPCTPFEVANGMPFWELSAKKPRFGDFFNEAMEADSKLIAGVVVGVCGGVFEDLKSLVDVGGGTGTMAKAIANAFPNINCTVFDQPHVVADLEGMAHNLGFVGGDMFEEIPPANAILLKVEPHNNGILIEYVPNFVCFELEEMLMGHTAGISEPQSSKDWLLPYAESSELDDIDTADCSEQILFTAFFEELARNIVKYDTVIWLTISLLLVSLGSWHHHAFISAL
>Chr1.g58131.m1
MTKTESGDDRGTEVTKTSVQSARFEIEKFIGTNNFAMWQCEVKDVLIQQGLLMVLEDMPITVKKEEWQRLNAHACSTIRLCLGKAQKYGVMNISSARDLWDTLESKYLKKSAENRLYLKSKLYRFQYKEGTKMIDHLEEFNKMIAELLNLEETIKDEDKALILMNSLPESYESFVTTWIYGRETLKFDEISSAVMNHEVRNLDRQERNNSEALMVRGRSKERRPATKKKSKSRSRGTSSNRKFLDKDECAFCHEKGHWKKDCPKIKAKNKEKKYEDSEANIVEADNEDFVCALTASDGINYMNHWVLDTGCTHHMTSQRHWFSSFKTLTGMVYMGDDNPCATKGIGSVKIRHHDGVVRELQGVRYVPNLKKNLISLGTLEDQGCKFYSEKQTLKVARGALVLMKAPRRGLLYLLEGTTIESPMAMAAASDQSDASCLWHMRLGHVGNKALQGLIKQGVLEGAKSGKIDFCEHCILGKQTKVSFGTAIHQTEGILDYVHSDVWGPAKNESLGGKRWFVSFIDDYSRRAWIYMMKHKHEVLDIFLEWKTTVERKTEKKLKVLRSDNGGEYTSDPFFEACKKEGITRHFSVRETPQQNGIAERLNRTLLEKVRCMLSQAKLHKRFWAEALSYACHILNRLPSSALNGKTPMEVWSGQIAQDYDRLKVFGCDAYYHVRENKLDPRARKAIFLGFNSGVKGFRLWSTELKKIVISRNVTFNETHMQLREDRKIVQEVELAKEPTEYSRDEDKLDNESHISVDQEEEQEEDSMINDVPDQDDCIAKRKGKREVGLPAKFKDCVSCIAYALPVIEVDIPTDFTEASRSKEAKNWHEAMDDEMLSLKKNRTWDLVELPKGRKAIGCKWVYAKKDGINNASSLRFKARLVAKGYAQKEGIDYNEIFSPVVKHSSIRILLALVAQFDLELVQLDVKTAFLHGNLAEEIYICQPEGYEEKGKKNLVCKLNKSLYGLKQSPRQWYLRFDKFMKDFGYTRSQYDHCVYHKKTRKGIYVYLLIYVDDMLIASSDQTEISELKTQMQNEFEMKDLGEAKKILGMEITRDRKKGLVCLSQKQYVEKLLHKFGIRDETKPVSTPLASHFKMSTQVCPKTDEERTKMKDVPYSSLVGGLMYAMVCTRPDIAHAVGLVSRFMHNPGKMHWEAARWILRYLHGTRSTGICFERNDKGIEKFSIGYVDSDFASDLDKRRSTTGYVFTMANGPICWRSILQPTVALSTTEAEYMAMAEAIKEAIWTLGLIADLGVKQRKIDVHCDSQSAIYLAKYQVHHARTKHIDVRYHFVREIIAEGEILVTKIASADNPADMLTKVVGASKFSHCLDLVHVRQVLTTPFHLMGAWLQMNGGDDPATIFTPFEMENGMPFWDLAAQEPRFGNLFDEAMEADSKLLGREVVEECGGVFEGLKSLVDVGGGTGTMAKAIANAFPSINCIVFDQPHVVADLQGTTHNLGFVGGDMFVEIPPANAILLKWILHDWSDEESMKILKKCREAILLSKNDEGNKKIIIIDIVVGHVDNKEKMVDKKSIETQLMFDMLMMSTVTGKERSESEWKKIFLAAGFTHYNITHTFGFRSLIELYP
>Chr1.g58175.m1
MPFWELNAKKPTFGGLFNEAMEADSKLIARVPVEECGGVFEGLKSLVDVEEGTGTMAKAIAYAFPNINCTVFDQPHVVADLEGMAHNLGFVGGDMFDKIPPANAILLKIRYLHLILDCLCKWIMHDWNEEESVKILKKCRESMLLCKNEGRKKIIIIDIVVGYDKKSIETQLMFDMLMMSTVTNKERSESEWENIFLAAGFIHYNITHTLSLRSLIEVYP
>Chr7.g33394.m1
MDYRFKGFLTLLIEEDPEFWTWLATARVPRIKFLSKNEGRKKFIIIDIIVNHVGNKKMVDKKSIETQLMFDTLMMATSAGKEHSESELAVGFTHYNITQYAWVEVSYPCCM
MSSSNAVGATSHELLGAQAQLWNHIFQFINSMSLKCAVQLGIADVIHNHGQPISLSELIAGLNVHPSKAHFVSRLMLILVHSNFFAQHHHVHHDRADVEEEEAVVLYSLTPSSRLLLKDGPLSTTPFLLAILDPVVTTPFHLMGAWLKINGGDDPAATCTPFEMENGMPFWELGAQEPRFGNLFNEAMEADSKLIGRVVVEECGGVFEGLKSLMDVGGGSGTMAKAIANAFPNINCTVFDQPHVVGGLQGTTHNLGFMGGDMFEEIPPANAILLKWIMHDWNDEESVTILKKCREAISLSKNEGGNKKIIIIDIVVGYVDNKKKMMDKKSIETQLMFDMLMMSIVHGKERSKLEWEKIFFAAGFTHYNITHTLGLRSLIEVYP
>Chr1.g58176.m1
MALSNREATSHELLGAQAHVWNHIFQFINSMSLKCAVQLGIPDVIHNHGQPISLSELIAGLNVHPSKAHFISRLMRILVHSNFFAQHHHVHHNHDNVEEEEAVELYSLTPASRLFLKDGPLNTTPFLLMILDPVMTDPFHSMGAWLQMNGGDDLVPRTPFEVANGMPFWELSAKKPRFGDSFNEAMEADSKLIARAVVEECGGVFEGLKSLVDVGGGTGTMAKAIANAFPNINCTVFDQPHVVANLEGTTHNLGFVGGDMFDKIPPANAILLKWILHDWNDEESVKILKKCREAMLVSKNEGRKKIIIIDMVVGYVNNKMQLDKKSIETQLMFDMLMMSTVTSNERSELEWEKIFLAAGFTHYNITHTLGLRSLIEVYL
>Chr1.g58124.m1
MGLSNGEVGATSHELLRAQGRIWNHMFQFTNSMALKCAAQLGIPDVIHSHGQPISLSELIAALNVHPSRAHFVSRLMRILVHSDFFAQHHHVHHDCDDVEDEEAVVLYSLTPTSRLLLKDGPSNTTPLILMILDPVLTTPFHLMGAWLQMNGGDGPATICTPFEMENGMPFWDLAAQEPRFGNLFDEAMEADSKRLGREVVEECGGVFEGLKSLVDVGGGTGTMAKAIANSFPSINCTVFDQPHVVADLQGITHNLGFVGGDMFVEIPPANAILLKWILHDWSDEESVKILKKCREAILLSKNDEGNKKIIIIDIVVGHVDNKEKMVDKKSIETQLMFDMLMMSIVTGKERSESEWKKIFLACWFHSL
>Chr1.g58121.m1
MGLSNGEVGATSHELLGAQAQIWNHMFQFINSMALKCAAQLGIPDVIHNHGQPISLSDLITALNVHPSRAHFVSRLMRILVHSNFFAQHHHVHHDCDDVEEEEAVVLYSLTPTSRLLLKDGPSNTTPLILMILDPVLTTPFQLMGAWLQMNGGDDPATICTPFEMENGMPFWDLAAQEPRFGNLFDEAMEADSKLLGREVVEECGGVFEGLKSLVDVGGGTGAMAKAIANAFPSINCIVFDQPHVVADLQGTTHNLGFVGGDMFVEIPPANAILLKWILHDWSDEESVKILKKCREAILLSKNDEGNKKIIIIDIVVGHVDNKEKMVDKKSIETQLMFDMLMMSAFTGKERSESEWKKIFLAASFTHYNITHTFGFRSLIELYP
>Chr1.g58171.m1
MGLSNGEVGAISHEDLGAQAHVWNHMFQFINSMSLKCAVQLGIPDVIHNHGQPISLSELIAGLNVHPSKAHFISRLMRILVHSDFFAQHHHVHHDRDDAEEEVAVVLYSLTPASGLFLKDGPLNTTPFLLMILDPVITDPFHSMSNWLHMNCGDDLVPCTPFEVANGMPFWELSAKKPRFGDFFNEAMEADSKLIAGVVVGVCGGVFEDLKSLVDVGGGTGTMAKAIANAFPNINCTVFDQPHVVADLEGMAHNLGFVGGDMFEEIPPANAILLKVEPHNNGILIEYVPNFVCFELEEMLMGHTAGISEPQSSKDWLLPYAESSELDDIDTADCSEQILFTAFFEELARNIVKYDTVIWLTISLLLVSLGSWHHHAFISAL
>Chr1.g58131.m1
MTKTESGDDRGTEVTKTSVQSARFEIEKFIGTNNFAMWQCEVKDVLIQQGLLMVLEDMPITVKKEEWQRLNAHACSTIRLCLGKAQKYGVMNISSARDLWDTLESKYLKKSAENRLYLKSKLYRFQYKEGTKMIDHLEEFNKMIAELLNLEETIKDEDKALILMNSLPESYESFVTTWIYGRETLKFDEISSAVMNHEVRNLDRQERNNSEALMVRGRSKERRPATKKKSKSRSRGTSSNRKFLDKDECAFCHEKGHWKKDCPKIKAKNKEKKYEDSEANIVEADNEDFVCALTASDGINYMNHWVLDTGCTHHMTSQRHWFSSFKTLTGMVYMGDDNPCATKGIGSVKIRHHDGVVRELQGVRYVPNLKKNLISLGTLEDQGCKFYSEKQTLKVARGALVLMKAPRRGLLYLLEGTTIESPMAMAAASDQSDASCLWHMRLGHVGNKALQGLIKQGVLEGAKSGKIDFCEHCILGKQTKVSFGTAIHQTEGILDYVHSDVWGPAKNESLGGKRWFVSFIDDYSRRAWIYMMKHKHEVLDIFLEWKTTVERKTEKKLKVLRSDNGGEYTSDPFFEACKKEGITRHFSVRETPQQNGIAERLNRTLLEKVRCMLSQAKLHKRFWAEALSYACHILNRLPSSALNGKTPMEVWSGQIAQDYDRLKVFGCDAYYHVRENKLDPRARKAIFLGFNSGVKGFRLWSTELKKIVISRNVTFNETHMQLREDRKIVQEVELAKEPTEYSRDEDKLDNESHISVDQEEEQEEDSMINDVPDQDDCIAKRKGKREVGLPAKFKDCVSCIAYALPVIEVDIPTDFTEASRSKEAKNWHEAMDDEMLSLKKNRTWDLVELPKGRKAIGCKWVYAKKDGINNASSLRFKARLVAKGYAQKEGIDYNEIFSPVVKHSSIRILLALVAQFDLELVQLDVKTAFLHGNLAEEIYICQPEGYEEKGKKNLVCKLNKSLYGLKQSPRQWYLRFDKFMKDFGYTRSQYDHCVYHKKTRKGIYVYLLIYVDDMLIASSDQTEISELKTQMQNEFEMKDLGEAKKILGMEITRDRKKGLVCLSQKQYVEKLLHKFGIRDETKPVSTPLASHFKMSTQVCPKTDEERTKMKDVPYSSLVGGLMYAMVCTRPDIAHAVGLVSRFMHNPGKMHWEAARWILRYLHGTRSTGICFERNDKGIEKFSIGYVDSDFASDLDKRRSTTGYVFTMANGPICWRSILQPTVALSTTEAEYMAMAEAIKEAIWTLGLIADLGVKQRKIDVHCDSQSAIYLAKYQVHHARTKHIDVRYHFVREIIAEGEILVTKIASADNPADMLTKVVGASKFSHCLDLVHVRQVLTTPFHLMGAWLQMNGGDDPATIFTPFEMENGMPFWDLAAQEPRFGNLFDEAMEADSKLLGREVVEECGGVFEGLKSLVDVGGGTGTMAKAIANAFPSINCIVFDQPHVVADLQGTTHNLGFVGGDMFVEIPPANAILLKWILHDWSDEESMKILKKCREAILLSKNDEGNKKIIIIDIVVGHVDNKEKMVDKKSIETQLMFDMLMMSTVTGKERSESEWKKIFLAAGFTHYNITHTFGFRSLIELYP
>Chr1.g58175.m1
MPFWELNAKKPTFGGLFNEAMEADSKLIARVPVEECGGVFEGLKSLVDVEEGTGTMAKAIAYAFPNINCTVFDQPHVVADLEGMAHNLGFVGGDMFDKIPPANAILLKIRYLHLILDCLCKWIMHDWNEEESVKILKKCRESMLLCKNEGRKKIIIIDIVVGYDKKSIETQLMFDMLMMSTVTNKERSESEWENIFLAAGFIHYNITHTLSLRSLIEVYP
>Chr7.g33394.m1
MDYRFKGFLTLLIEEDPEFWTWLATARVPRIKFLSKNEGRKKFIIIDIIVNHVGNKKMVDKKSIETQLMFDTLMMATSAGKEHSESELAVGFTHYNITQYAWVEVSYPCCM
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University