Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Apostasia shenzhenica
Species name
Apostasia shenzhenica is a species of monocot in the family Orchidaceae (orchid family).
Species info
Gene sequence info
Apostasia_shenzhenica.COMT.fa
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>rna-gnl|WGS:PEFY|mrna.Ash018969
MKGHAIVFKKLLEKYEGFAGASGVVVDVGGGTGVALKLIRGRSPSRVEHIEGDMFESVPAGDTILMKMILHDWCDEDCLKILKNCRKALPETGKVVSVEPILPESPEASTEAHCVFPVDMLMLLESPSGKERTKKEFESLTIESGFTGFRPVCSFATVWVMEFTK
>rna-gnl|WGS:PEFY|mrna.Ash009392
MPFMMTHGKTFWDLAGDQPEFNDLFNEGMARDSWVVMDVVTHIGGSAFHGMTTLVDVGGGTGKTVAALVETFPELRCTVFDLPHVVNKAPKIPGVEFVGGDMFVEVPRVDAVLLKVSMSVLFSFFFCFFFCFGSQLVSFCFYKGFFCWFLGWSVQKYLQT
>rna-gnl|WGS:PEFY|mrna.Ash009307
MDAEKDACNCAIKLASLSVLPMTIFSAIELGLFQHIAAAGPGEELTADELAARIGATNPLAASMLERILRLLASHSVFTCSTSTDAAGRTVHRYGPTPVCRYLAPSDDGVSLAALCLMASDRVSMESWYHVKDSVLHGGVPFNMAHGGLTEFEYHNIDPRFSKVFNEGMRSHSIIIMKNILEIYQGFDDVTVLVDVGGGTGGTLSMITAKHSHIKGINFDLPHVIAQAPPLPGVEHIGGDVFASVPSGDAIFMKWILHDWSDEDSLKILSNCRKALPEKGKVIVVDSILPESPESSDFAHCVFHVDLIMMLESPSGKERTEKEFRDLAMNAGFSAFNVVCNFSNASLMEFTK
>rna-gnl|WGS:PEFY|mrna.Ash019588
MAASGDGKAAIAPPEDRRQIKKAARLAMMEISNMLSVPMALNAVVRLGVPNAMWQSGSNSPLTAGELLSRLRPTLPSTASPENLQRLLRLLASHGVFAEHLAGADRRYNLTEVGRTLVDEDEEIGGISYAPYVLQHHQDSLLRAWPLLHEAVRDPAGPEPFARANGGVPAYAYYGGNEAANDLMRKAMWGVSAPFMESFLDEYGVTGGFEGVKVLVDVGGSSGACLQMILRRIPSITAGVNFDLPEVVAGAPVFPGAISDPLVLSFWGWRMTVSTLLVCLLLLKWILTTWMDEECKLILKNCYAAVPAGGKVIICEPVLPEETDGSCRTRALLEGDVFVMTIYRSQGKERTEDEFRQLALAAGFSGFRALYFDPFYTVMELLKE
>rna-gnl|WGS:PEFY|mrna.Ash012060
MAAPVLASMAVPRPCIVLPMSSAASRDGIVPVSAAAGRWRSGMLPGFSGLRASVSREMLKPASMVIRRAKVVCEAQDTTVEVPDVTEETWQSLVMECDKTVLVEFWAPWCEPCSTIHPVIGSVSAAYAGMIKCFKINTDENPEITAKYEIRSIPTVVIFKNGEKMEKVIGAVPESTLISFIEGYLDRNSYPSQPMETYKEEEEPFLRAMSLMSMSAIGTVVKAIIELGVLDIIAAAGPGAGISLQDIVAQLNTTKPIASADALERMLKFLVNYSILKCRDEVDDKGQSRRLYGLEPVYQFLLKSRDGMSLAPLLLHEQNLALTTAWHHLKVAVTEGDVPFDHVHGTNVNDHRVRHFDTLGRLFHQAMAENTAILMNKILETYKGFEEIAELLDVGGGYGATLALILSRYPHIKGINFDLPYVISEAKLIPGVEHVSGDMFISIPTAKTIFLKILKNCWKALPENGKVLIAEFVLPSVPKANLEAQVVHHIDLCMLAYTSGGKERTKEEFENLATEAGFTGLSITSTIVAFTIIELSK
>rna-gnl|WGS:PEFY|mrna.Ash000034
MAMHAVMQAGALVRSDRVHSTTCTAALSLIRCEVGERKSLHKRSQLSPKNGRSPPLPLLARRNPDMAYDRQANLVGGAVDDDEAACLYALQLVSLSVLPMTLKAAIELGLLEIITGAGAGAQLSAAEIAARIPASGNPQASVMLDRILRLLASYSVLTCSLSPSGELRYGAAPACKFLTANDDGFSMAGLALMNQDRVLIESWYHLKDAVLEGGIPFNKAYGMTAFEYHGKDLRFNTVFNNGMSGHSSIITGKLLEIYRGFEGLGSLVDVGGGVGATMATITAVYPSIRGINFDLPHVISDAPVLPGSFFIFLCFSVFS
>rna-gnl|WGS:PEFY|mrna.Ash021832
MRYLANMRLVNVREAAGDQFYTLTPASSTYLRVDSEKSFVPFARFLIKVEMIAPFLALDACVVAGDEDSDVPAIERLGGKSFYDMVALDPEAFHVTMRAMVTRTAEAVAEGSRAVFEGVTTVVDVGGGDGMMAATIARAFPAIKCVVLELAHVVERAPERVGVEFVAGDMFVSLPRADALLFTRVLHNWSDDKVTEILRRCMEAIDEDKGKVIIIDMVIDDSKEDPDVHQIKLSRDIIMMAYHGGKERTKEEWRSLLAKSLDVARHLTASAAYLADLAVSNSFYHRPPPTLPAAYLADLAVAGLLPRRPRCVGLLPPLFLPLKAL
>rna-gnl|WGS:PEFY|mrna.Ash017193
MCLKCAVELHIPDAILSHVMVSTVSELSAALSIPPSKTRCLCVLMRLLSHEGAFRISCQMPFGKTLWDLAGDQPEFNGLFNKGMASDCRVVMDVVTHIGGSSFHGITTLVDVGGGTGKTVAALVEAFPELLCTVFDLPHVANTAPKIPGAEFIGGDMFVGVPPADTILL
>rna-gnl|WGS:PEFY|mrna.Ash015466
MVSAAAESDQEQKKLERPMAFVDPEEEAYLHAMQLVSFSALPMTLKVATELGLLQLISAAVPNHHLSSDDLAALLRPSDPLFASSMIDRILQLLASYSVLTVSHSADAVDGRSVRRYSAAPVCKYLAPSIDGAASIAGITLLLQDKILMENWYYLKDSILRGRSDQTTSSSPFEMAYGMNMFEYCGRDRRFNSLFNEGMRGNSAIIMNKVLHGRHSCGTFDDVRVLVDVGGGLGATLSMITARHPHILGINFDLPHVVSQAPPLPGVDHIGGDMFEQVPDGDAILLKWILHDWSDEQSVNVLKNCYKALPANGKVLILEYILPDSPEPTREAQVVFHADLMMLAHNPGGKERTEKEFKDLADRSGFSRFKANFIFANAWIIELQK
MKGHAIVFKKLLEKYEGFAGASGVVVDVGGGTGVALKLIRGRSPSRVEHIEGDMFESVPAGDTILMKMILHDWCDEDCLKILKNCRKALPETGKVVSVEPILPESPEASTEAHCVFPVDMLMLLESPSGKERTKKEFESLTIESGFTGFRPVCSFATVWVMEFTK
>rna-gnl|WGS:PEFY|mrna.Ash009392
MPFMMTHGKTFWDLAGDQPEFNDLFNEGMARDSWVVMDVVTHIGGSAFHGMTTLVDVGGGTGKTVAALVETFPELRCTVFDLPHVVNKAPKIPGVEFVGGDMFVEVPRVDAVLLKVSMSVLFSFFFCFFFCFGSQLVSFCFYKGFFCWFLGWSVQKYLQT
>rna-gnl|WGS:PEFY|mrna.Ash009307
MDAEKDACNCAIKLASLSVLPMTIFSAIELGLFQHIAAAGPGEELTADELAARIGATNPLAASMLERILRLLASHSVFTCSTSTDAAGRTVHRYGPTPVCRYLAPSDDGVSLAALCLMASDRVSMESWYHVKDSVLHGGVPFNMAHGGLTEFEYHNIDPRFSKVFNEGMRSHSIIIMKNILEIYQGFDDVTVLVDVGGGTGGTLSMITAKHSHIKGINFDLPHVIAQAPPLPGVEHIGGDVFASVPSGDAIFMKWILHDWSDEDSLKILSNCRKALPEKGKVIVVDSILPESPESSDFAHCVFHVDLIMMLESPSGKERTEKEFRDLAMNAGFSAFNVVCNFSNASLMEFTK
>rna-gnl|WGS:PEFY|mrna.Ash019588
MAASGDGKAAIAPPEDRRQIKKAARLAMMEISNMLSVPMALNAVVRLGVPNAMWQSGSNSPLTAGELLSRLRPTLPSTASPENLQRLLRLLASHGVFAEHLAGADRRYNLTEVGRTLVDEDEEIGGISYAPYVLQHHQDSLLRAWPLLHEAVRDPAGPEPFARANGGVPAYAYYGGNEAANDLMRKAMWGVSAPFMESFLDEYGVTGGFEGVKVLVDVGGSSGACLQMILRRIPSITAGVNFDLPEVVAGAPVFPGAISDPLVLSFWGWRMTVSTLLVCLLLLKWILTTWMDEECKLILKNCYAAVPAGGKVIICEPVLPEETDGSCRTRALLEGDVFVMTIYRSQGKERTEDEFRQLALAAGFSGFRALYFDPFYTVMELLKE
>rna-gnl|WGS:PEFY|mrna.Ash012060
MAAPVLASMAVPRPCIVLPMSSAASRDGIVPVSAAAGRWRSGMLPGFSGLRASVSREMLKPASMVIRRAKVVCEAQDTTVEVPDVTEETWQSLVMECDKTVLVEFWAPWCEPCSTIHPVIGSVSAAYAGMIKCFKINTDENPEITAKYEIRSIPTVVIFKNGEKMEKVIGAVPESTLISFIEGYLDRNSYPSQPMETYKEEEEPFLRAMSLMSMSAIGTVVKAIIELGVLDIIAAAGPGAGISLQDIVAQLNTTKPIASADALERMLKFLVNYSILKCRDEVDDKGQSRRLYGLEPVYQFLLKSRDGMSLAPLLLHEQNLALTTAWHHLKVAVTEGDVPFDHVHGTNVNDHRVRHFDTLGRLFHQAMAENTAILMNKILETYKGFEEIAELLDVGGGYGATLALILSRYPHIKGINFDLPYVISEAKLIPGVEHVSGDMFISIPTAKTIFLKILKNCWKALPENGKVLIAEFVLPSVPKANLEAQVVHHIDLCMLAYTSGGKERTKEEFENLATEAGFTGLSITSTIVAFTIIELSK
>rna-gnl|WGS:PEFY|mrna.Ash000034
MAMHAVMQAGALVRSDRVHSTTCTAALSLIRCEVGERKSLHKRSQLSPKNGRSPPLPLLARRNPDMAYDRQANLVGGAVDDDEAACLYALQLVSLSVLPMTLKAAIELGLLEIITGAGAGAQLSAAEIAARIPASGNPQASVMLDRILRLLASYSVLTCSLSPSGELRYGAAPACKFLTANDDGFSMAGLALMNQDRVLIESWYHLKDAVLEGGIPFNKAYGMTAFEYHGKDLRFNTVFNNGMSGHSSIITGKLLEIYRGFEGLGSLVDVGGGVGATMATITAVYPSIRGINFDLPHVISDAPVLPGSFFIFLCFSVFS
>rna-gnl|WGS:PEFY|mrna.Ash021832
MRYLANMRLVNVREAAGDQFYTLTPASSTYLRVDSEKSFVPFARFLIKVEMIAPFLALDACVVAGDEDSDVPAIERLGGKSFYDMVALDPEAFHVTMRAMVTRTAEAVAEGSRAVFEGVTTVVDVGGGDGMMAATIARAFPAIKCVVLELAHVVERAPERVGVEFVAGDMFVSLPRADALLFTRVLHNWSDDKVTEILRRCMEAIDEDKGKVIIIDMVIDDSKEDPDVHQIKLSRDIIMMAYHGGKERTKEEWRSLLAKSLDVARHLTASAAYLADLAVSNSFYHRPPPTLPAAYLADLAVAGLLPRRPRCVGLLPPLFLPLKAL
>rna-gnl|WGS:PEFY|mrna.Ash017193
MCLKCAVELHIPDAILSHVMVSTVSELSAALSIPPSKTRCLCVLMRLLSHEGAFRISCQMPFGKTLWDLAGDQPEFNGLFNKGMASDCRVVMDVVTHIGGSSFHGITTLVDVGGGTGKTVAALVEAFPELLCTVFDLPHVANTAPKIPGAEFIGGDMFVGVPPADTILL
>rna-gnl|WGS:PEFY|mrna.Ash015466
MVSAAAESDQEQKKLERPMAFVDPEEEAYLHAMQLVSFSALPMTLKVATELGLLQLISAAVPNHHLSSDDLAALLRPSDPLFASSMIDRILQLLASYSVLTVSHSADAVDGRSVRRYSAAPVCKYLAPSIDGAASIAGITLLLQDKILMENWYYLKDSILRGRSDQTTSSSPFEMAYGMNMFEYCGRDRRFNSLFNEGMRGNSAIIMNKVLHGRHSCGTFDDVRVLVDVGGGLGATLSMITARHPHILGINFDLPHVVSQAPPLPGVDHIGGDMFEQVPDGDAILLKWILHDWSDEQSVNVLKNCYKALPANGKVLILEYILPDSPEPTREAQVVFHADLMMLAHNPGGKERTEKEFKDLADRSGFSRFKANFIFANAWIIELQK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University