Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Punica granatum
Species name
Pomegranate (Punica granatum) is a species of eudicot in the family Lythraceae (loosestrife family).
Species info
Gene sequence info
Punica_granatum.COMT.fa
Download
>rna-XM_031523214.1
MYITPCTTPAMFEATINSCNQPRLVSVKFLLSLRDKAVHSLVSKQNILTKPGRRIKKLLSTETMNSTASAASVDHSISKLEDEPYLQALTISTLFSFPMILKAAVELDVLGIISRAGSAGVSAGDIASHIPTKDPDASRRVDRMLCLLAVNSILTCSLSENDERGVQRIYRLSPTGRYFVQQGDEGSAASMLALNAHPITMEVWSHAKDTILGGGNEFKKVHKKSAFHYTNIDPTYNQIFNQAMADLTTKVMNQVLQIYGGFERLGSLVDVAGGTGKCLAVITSKYPPIKGINFDVPHVVATAPFYPGIKHVGGDMFASVPKGEAIMIKDALHNWDDEHCIKVLKNCYESLPEKGKAIIINVLMPKAPDSTLASRYITQLDATMLIQLGSHERTQKQFEALCMASGFSEFWVADHLAYSVWAIMEFRK
>rna-XM_031522763.1
MDSTGETSTMNPAQPQAPKVDEENTYLLAMQLTRASELPMVLKTAIELSLLEIISKFASGPMSAKEISAHLPTQNPDAPVMIDRILRLLATRNIITCSLLELLAVASNAAERAERVYGPAPVCKFLTQNEYGVSLAPFCLMIQDKVLMESCYYLKDAILEGGVPFDKVYGTSSFEYYRTNSRYNQIINWGLSNQSAIVMTKILETYKGFEGLSALVDVSGGIGATLNMIISKYPTIKGINFDMRQVIEEAPSHPGMLFYAYPFIVYAHSLEMCREWSDRHCLKILQNCYEALPKDGKVIVAECLLPEVPDTSPASKLVVHADCIKLSYNSGGKERTKEEFEALAKGAGFKGFGIACKAFETCIVEFLKQGN
>rna-XM_031537283.1
MSPAQSQLVAPGSLCEEDEACLQAMIFSSNHVFPMVLNAATQLGVFDILAQAGPKAQLSPSEIASQLKTTDPDVAARLDRMLRLLASHSLLTSSLKNLEDGRVERLYGLTPAAQFFVRKEGDESLASLTALSCHPAPQQVWLHFKDAVIEGGSQFKKVHGVSIFEYMDSNPAFNTIFNKGMVALSTIIMKKILEIYDGFEGLSSLVDVAGGTGKSLNMIVSKYPSIKGINFDLPHVTKEAPPYPGIEHVGGSMFSSIPKADAIMIKDALHNWSDENCVKILKNCYEALPCKGKVIVLDVLLPEAPESSMYSMYVSRLDNTMLMQPGGRERTEREFKALADAAGFSNFRVACCACTIWFVMELYKS
>rna-XM_031534676.1
MAQVDRGSSLISHPDVDKDDEAIVYASVLTSSFIVPMVLNAAIELGVLDTISEAGPEKKLSSDEIASIIGAQNPCMGSTLDRMLRLLATHSVLNCSTRDAKNEGKVERVYGLTPVGRLFVQNKAGGSLVGFLGFSHNQAILDIRHYIKDAVLKGGIPFKMAHGVPAFEYMRSDPEFSGAFNTAVSFHSSIVTEKILKRYTGFEDLRVLIDVGGGRGTTLNMIVSKYPHIKGINFDLPQVIQDASPFEGIEHIGGDMFKEVPRGEAILLKRTCHNWSDEKCLKLLKNCHEALEDNGKLIIIDSIMSEGCDSSLIDKYVSHYDNIMFTQHEGKERTEKEFRLLGEQARFMDFRVVGCACAHWVMELRK
>rna-XM_031535490.1
MENRMTMSTVEEAGEQRSSLDALQLAGASVLPMALKTAVELGVLDIIHRAGPGAELSASQIVSKLDSKNPRAAYLLDHMLCLLATHSVLTCSVSPAHQSDGSQSQSCQRVYGLAPVSKYFIKDEQGGSLSPLLHLIQHRVVLDMWFHWKDAILEGGLPFEKAHGMSTMSYIHKDARFGENFWETMNDLNKLFVVQMLEAYKGFEGLRSLVDVGGRDGSVLKMIISKYPSIKGVNFDLPSVIEKAPSIEGVENVGGDMIQGVPKGEAILIKWVLHSWTDEECVKVFKNCHESLPENGKVMVVDFVIPEAPDASLAVQSVFQLCMFMLSMNPNRNERTEKDFDSLAKAAGFAGVRVFPCGYGFSLIEIYKN
>rna-XM_031536132.1
MGSTGQSAETQMTPTQVSDEEANLFAMQLASASVLPMVLKAALELDLLEIMAKSCPARGGYISSSDIAVQLPTINPEAPVTLDRVLRLLASYSVLTCKLRDLPDGKVERLYGLGPVCKFLTKNEDGVSIAPLCLMNQDKILMESWYHLKDAILEGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTITMKKILETYKGFEGLNTIVDVGGGTGAVLSMIVAKYPSIKGINFDLPHVIEDAPSLPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYAALPDHGKVIVAECILPLSPDPSLATKGVIHIDCIMLAHNPGGKERTEKEFEALAKGAGFEGFRVACCAFNTYVMEFLKKA
>rna-XM_031537284.1
MGSSSTENNHGSKHHQEQDDPCLTAMLMSSYHVFPVILYAAVDLDLFGIIAREGRGGFMSPSEVACHLPSRCPESAEILDRVLRLLASHSLLSYCTETDRDGKVVMKYGIAPAGKYYVRAADGDSAASLSLFAHHRAIVDVMLGMKEAILGGGNLFQKFHGMTIFQYMNDSILNKYFNDAMADLSFIQTKKVLEVYNGFEGLSVLVDVGGGKGATLHAIISKYPSIKGINFDLPQVIQHAPAYPGIEHVGGDMFEGVPKGDAIMIKGTCHNWSDESCLKFLRNCYNAIPENGKVIIIDFIMPDEPETTDEAKYVAMLDNAMFIQPGGKERTERQFEALCRGSGFSGFKVAAKAIKALGVIEFTK
>rna-XM_031535489.1
MRNHSMAIPAINEEEEEQRSSLYAWQLASSSVLPMVLKAAVELGVLEVIAAAGPGSMLSASQISSELKSTNPRAAYVLDQMLCLLAAHSVLTCSVSPSECSTRAGQSQTSERLYGLSPVSKCFIRDQEGGSLSPLLHLIQDRTMLDMWFHLKDAVLQGGLPFERASGTSAASYMGRDPRLSEIFRGSMKDFNKLFVIKMVETYNGFEGLSTLVDVGGGDGSILNTIISKYPSIKGINFDLTSTIERSPTYQGIENVAGDMFQSIPKGDAIFMKWMLHNWSDEDCVKILKNCYVALPVNGKVIVVELVIPEVPDPGSASRSLFQMHMFMMNLNPGGKERTEKEFDILAKEAGFSGVRVVSCAYDFSFVEIYKDEKSC
>rna-XM_031537282.1
MSPSLKPLLPPSGSLSEEDEACLQAMLFSSAHVVPMVLNAATQLGLFDIINRAGPKAQLLPSQIASELETAVDCTDDDVASRLDRMLRLLASHSLLTCSVKTLEDGRVERRYGLAPSSRFFVKAESEEPSLASLVALHSHPAPQQAWFHFKDAVLEGGNQFKKVHGMSIFEYMDNDPAFNTIFNSSMVAISNLMMKRILEIYDGFEGLSSLVDVAGGTGKCLSMITSKYPSIKGINFDLPHVIKEAPSYPGIEHVGGSMFSSNIPKADAIMIKDALHNWTDEDCVKILKNCYDTLPSKGKLILIYHLLLEEPDTSISSMYASRLDNTMMMQPNGKERTESEFRALSEAAGFSNCKFVCCACNIWAVMELYKS
>rna-XM_031540929.1
MADETSDRRNQARIKILELANMISVPMSLNAAVRLKVPDAIWAGGSNSPLSASQILAVIRSSSSSSSGGDPENLQRILRMLASYGVFEEHLVGSGERKYSLTDVGKTLVTDANGLSYADYVLQHHQDELLKAWPLVHEAVLDPSVEPFARANGEPAYSYYGKKPEMNVLMLRAMSGLSVPFMRAVLDSGYDGFAGVKRLVDVGGSAGDCLRMIMGKHPNVTEGINFDLPEVVAKAPSIPGVTHVGGDMFEFIPTGDAIFMKWILTTWTDDEVKSIMENCCKALPAGGKLIACEPVLPKVSDDSHRTRALLEGDIFVMTIYRTKGKHRTEEEFRKLGQLAGFPHFRALYIDYFYTVLEFQK
>rna-XM_031546844.1
MEKEVWQSVTINGDDEEATSWPQALQITSSYMLPVILNFTVELDVFGIIARAGGTRISASEIASEIPAESTGINFDLPRVIRDAPSYSGIEHGGGDMFVTVLKGDAIMIRTAIITKKGKVIAITILMPEAPDSSSVSKYITQMDNLMLMTLESHERTLKQLETLCKVSGFSEFRVADKLTRSIWAVIEFCR
>rna-XM_031549797.1
MLESKLAKPDKVHTDENGADMMTKSLAKEKLHVCRNITANALVEFWNIGSGIGNGQGASGSELVALKLSKVSSSNLVNRENSLMGELWYHVKDAVLEGGLPFSKAYGGKTLFDYIPGDPMLKEVFNGAMSDNTSIVQKKILETYEGFMCLRTIVDVGGGTGTMLNLIVTKYPSIKGINFDLPHVIAEAPSYPGVEHVGGDMFISVPKGDAIFMKWISHNWGDEPLMKLLKNCHDALPQNGKVILADHILPQIPDTSIDTKVAFHLDSLMLVLCPGGKERTMEELEELAKRAGFRGFRVTSKVYGIAIMELFKEA
MYITPCTTPAMFEATINSCNQPRLVSVKFLLSLRDKAVHSLVSKQNILTKPGRRIKKLLSTETMNSTASAASVDHSISKLEDEPYLQALTISTLFSFPMILKAAVELDVLGIISRAGSAGVSAGDIASHIPTKDPDASRRVDRMLCLLAVNSILTCSLSENDERGVQRIYRLSPTGRYFVQQGDEGSAASMLALNAHPITMEVWSHAKDTILGGGNEFKKVHKKSAFHYTNIDPTYNQIFNQAMADLTTKVMNQVLQIYGGFERLGSLVDVAGGTGKCLAVITSKYPPIKGINFDVPHVVATAPFYPGIKHVGGDMFASVPKGEAIMIKDALHNWDDEHCIKVLKNCYESLPEKGKAIIINVLMPKAPDSTLASRYITQLDATMLIQLGSHERTQKQFEALCMASGFSEFWVADHLAYSVWAIMEFRK
>rna-XM_031522763.1
MDSTGETSTMNPAQPQAPKVDEENTYLLAMQLTRASELPMVLKTAIELSLLEIISKFASGPMSAKEISAHLPTQNPDAPVMIDRILRLLATRNIITCSLLELLAVASNAAERAERVYGPAPVCKFLTQNEYGVSLAPFCLMIQDKVLMESCYYLKDAILEGGVPFDKVYGTSSFEYYRTNSRYNQIINWGLSNQSAIVMTKILETYKGFEGLSALVDVSGGIGATLNMIISKYPTIKGINFDMRQVIEEAPSHPGMLFYAYPFIVYAHSLEMCREWSDRHCLKILQNCYEALPKDGKVIVAECLLPEVPDTSPASKLVVHADCIKLSYNSGGKERTKEEFEALAKGAGFKGFGIACKAFETCIVEFLKQGN
>rna-XM_031537283.1
MSPAQSQLVAPGSLCEEDEACLQAMIFSSNHVFPMVLNAATQLGVFDILAQAGPKAQLSPSEIASQLKTTDPDVAARLDRMLRLLASHSLLTSSLKNLEDGRVERLYGLTPAAQFFVRKEGDESLASLTALSCHPAPQQVWLHFKDAVIEGGSQFKKVHGVSIFEYMDSNPAFNTIFNKGMVALSTIIMKKILEIYDGFEGLSSLVDVAGGTGKSLNMIVSKYPSIKGINFDLPHVTKEAPPYPGIEHVGGSMFSSIPKADAIMIKDALHNWSDENCVKILKNCYEALPCKGKVIVLDVLLPEAPESSMYSMYVSRLDNTMLMQPGGRERTEREFKALADAAGFSNFRVACCACTIWFVMELYKS
>rna-XM_031534676.1
MAQVDRGSSLISHPDVDKDDEAIVYASVLTSSFIVPMVLNAAIELGVLDTISEAGPEKKLSSDEIASIIGAQNPCMGSTLDRMLRLLATHSVLNCSTRDAKNEGKVERVYGLTPVGRLFVQNKAGGSLVGFLGFSHNQAILDIRHYIKDAVLKGGIPFKMAHGVPAFEYMRSDPEFSGAFNTAVSFHSSIVTEKILKRYTGFEDLRVLIDVGGGRGTTLNMIVSKYPHIKGINFDLPQVIQDASPFEGIEHIGGDMFKEVPRGEAILLKRTCHNWSDEKCLKLLKNCHEALEDNGKLIIIDSIMSEGCDSSLIDKYVSHYDNIMFTQHEGKERTEKEFRLLGEQARFMDFRVVGCACAHWVMELRK
>rna-XM_031535490.1
MENRMTMSTVEEAGEQRSSLDALQLAGASVLPMALKTAVELGVLDIIHRAGPGAELSASQIVSKLDSKNPRAAYLLDHMLCLLATHSVLTCSVSPAHQSDGSQSQSCQRVYGLAPVSKYFIKDEQGGSLSPLLHLIQHRVVLDMWFHWKDAILEGGLPFEKAHGMSTMSYIHKDARFGENFWETMNDLNKLFVVQMLEAYKGFEGLRSLVDVGGRDGSVLKMIISKYPSIKGVNFDLPSVIEKAPSIEGVENVGGDMIQGVPKGEAILIKWVLHSWTDEECVKVFKNCHESLPENGKVMVVDFVIPEAPDASLAVQSVFQLCMFMLSMNPNRNERTEKDFDSLAKAAGFAGVRVFPCGYGFSLIEIYKN
>rna-XM_031536132.1
MGSTGQSAETQMTPTQVSDEEANLFAMQLASASVLPMVLKAALELDLLEIMAKSCPARGGYISSSDIAVQLPTINPEAPVTLDRVLRLLASYSVLTCKLRDLPDGKVERLYGLGPVCKFLTKNEDGVSIAPLCLMNQDKILMESWYHLKDAILEGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTITMKKILETYKGFEGLNTIVDVGGGTGAVLSMIVAKYPSIKGINFDLPHVIEDAPSLPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYAALPDHGKVIVAECILPLSPDPSLATKGVIHIDCIMLAHNPGGKERTEKEFEALAKGAGFEGFRVACCAFNTYVMEFLKKA
>rna-XM_031537284.1
MGSSSTENNHGSKHHQEQDDPCLTAMLMSSYHVFPVILYAAVDLDLFGIIAREGRGGFMSPSEVACHLPSRCPESAEILDRVLRLLASHSLLSYCTETDRDGKVVMKYGIAPAGKYYVRAADGDSAASLSLFAHHRAIVDVMLGMKEAILGGGNLFQKFHGMTIFQYMNDSILNKYFNDAMADLSFIQTKKVLEVYNGFEGLSVLVDVGGGKGATLHAIISKYPSIKGINFDLPQVIQHAPAYPGIEHVGGDMFEGVPKGDAIMIKGTCHNWSDESCLKFLRNCYNAIPENGKVIIIDFIMPDEPETTDEAKYVAMLDNAMFIQPGGKERTERQFEALCRGSGFSGFKVAAKAIKALGVIEFTK
>rna-XM_031535489.1
MRNHSMAIPAINEEEEEQRSSLYAWQLASSSVLPMVLKAAVELGVLEVIAAAGPGSMLSASQISSELKSTNPRAAYVLDQMLCLLAAHSVLTCSVSPSECSTRAGQSQTSERLYGLSPVSKCFIRDQEGGSLSPLLHLIQDRTMLDMWFHLKDAVLQGGLPFERASGTSAASYMGRDPRLSEIFRGSMKDFNKLFVIKMVETYNGFEGLSTLVDVGGGDGSILNTIISKYPSIKGINFDLTSTIERSPTYQGIENVAGDMFQSIPKGDAIFMKWMLHNWSDEDCVKILKNCYVALPVNGKVIVVELVIPEVPDPGSASRSLFQMHMFMMNLNPGGKERTEKEFDILAKEAGFSGVRVVSCAYDFSFVEIYKDEKSC
>rna-XM_031537282.1
MSPSLKPLLPPSGSLSEEDEACLQAMLFSSAHVVPMVLNAATQLGLFDIINRAGPKAQLLPSQIASELETAVDCTDDDVASRLDRMLRLLASHSLLTCSVKTLEDGRVERRYGLAPSSRFFVKAESEEPSLASLVALHSHPAPQQAWFHFKDAVLEGGNQFKKVHGMSIFEYMDNDPAFNTIFNSSMVAISNLMMKRILEIYDGFEGLSSLVDVAGGTGKCLSMITSKYPSIKGINFDLPHVIKEAPSYPGIEHVGGSMFSSNIPKADAIMIKDALHNWTDEDCVKILKNCYDTLPSKGKLILIYHLLLEEPDTSISSMYASRLDNTMMMQPNGKERTESEFRALSEAAGFSNCKFVCCACNIWAVMELYKS
>rna-XM_031540929.1
MADETSDRRNQARIKILELANMISVPMSLNAAVRLKVPDAIWAGGSNSPLSASQILAVIRSSSSSSSGGDPENLQRILRMLASYGVFEEHLVGSGERKYSLTDVGKTLVTDANGLSYADYVLQHHQDELLKAWPLVHEAVLDPSVEPFARANGEPAYSYYGKKPEMNVLMLRAMSGLSVPFMRAVLDSGYDGFAGVKRLVDVGGSAGDCLRMIMGKHPNVTEGINFDLPEVVAKAPSIPGVTHVGGDMFEFIPTGDAIFMKWILTTWTDDEVKSIMENCCKALPAGGKLIACEPVLPKVSDDSHRTRALLEGDIFVMTIYRTKGKHRTEEEFRKLGQLAGFPHFRALYIDYFYTVLEFQK
>rna-XM_031546844.1
MEKEVWQSVTINGDDEEATSWPQALQITSSYMLPVILNFTVELDVFGIIARAGGTRISASEIASEIPAESTGINFDLPRVIRDAPSYSGIEHGGGDMFVTVLKGDAIMIRTAIITKKGKVIAITILMPEAPDSSSVSKYITQMDNLMLMTLESHERTLKQLETLCKVSGFSEFRVADKLTRSIWAVIEFCR
>rna-XM_031549797.1
MLESKLAKPDKVHTDENGADMMTKSLAKEKLHVCRNITANALVEFWNIGSGIGNGQGASGSELVALKLSKVSSSNLVNRENSLMGELWYHVKDAVLEGGLPFSKAYGGKTLFDYIPGDPMLKEVFNGAMSDNTSIVQKKILETYEGFMCLRTIVDVGGGTGTMLNLIVTKYPSIKGINFDLPHVIAEAPSYPGVEHVGGDMFISVPKGDAIFMKWISHNWGDEPLMKLLKNCHDALPQNGKVILADHILPQIPDTSIDTKVAFHLDSLMLVLCPGGKERTMEELEELAKRAGFRGFRVTSKVYGIAIMELFKEA
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University