Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Punica granatum
Species name
Pomegranate (Punica granatum) is a species of eudicot in the family Lythraceae (loosestrife family).
Species info
Gene sequence info
Punica_granatum.ROMT.fa
Download
>rna-XM_031534816.1
MEGSGMNKTISGNARGSLVHAQTHIWNHIFSFVKSMSLKCAVQLGIPDIIHNHGGPMTINELVSALSVHPGKAHCISRLMRILVHSGFFEQTSLRVVAQDDREEVGYALNNVSSLLLKDNPLSTRPYALAMLDPALMNSGHCLGAWFLNDDPTPFSTEHGSLFFEHMGNGPEQNLLFNNAMASDARLLATEVLGSDCKRAFEGLRSLVDVGGGTGTMAKAIAAEFPDLECTVFDLPHVVAGLKGTKNVKYVGGNMFKEIPPADAYLLKWIIHDWSDEESVKILKRCKDGLREGKGCKLIIVDIVLGFGKEDEESVETQLFCDMQLMIILTGKERTEREWAKLFVEAGFRDYKITPMLGLRSLIEVYP
>rna-XM_031534050.1
MGSFSSNDGAKEAMQRLLQAQAHIWKHCYSYVSSMCLKCALELGIPEAIHSHSGPMTLNQLVEAIPVRPGKAHCLHRVMRVLVHSGFFELQIRKRGGAHEDEEVYSLNNESKLLLRDNPVNLDPFVLFTLDPITLKPMDCLSTWFQNDDLCTVETAHGMISWDIMVSNPKFGSLFNSAMASDSRLVARVVTDSCKWVFEGLKSLVDVGGGTGETGKAIADTFPDMEYTVFDLPHVVAGLQGTRNLKYVGGDMFNEKIPTADAVLLKWILHDWTDEESVQILKKCKEAIPRKEEGGKLIIIEMVMDNPAVGDEKTKETHLLADVFIMNIMGLERNTEEWAKLFADAGFSSYKISPVLGLRSVIEVYP
>rna-XM_031534421.1
MEDSGMSKTMGGNARGSLTHAQAHIWNHIFSFINSMSLKCALQLGIPDIIHNHGGPMTIGELVSALSVHSGKAHCIFHLMRILVHSGFFEQTSLRVVAEDDREEVGYALNNVSSLLLKDNPLSTRPFALAMLDPTLTDPWHCLGAWFLNDDPTPFSTEHGSLIWEHMGNEPEQNLRFNNAMASDARLVATEVLGSDCKRAFEGLRSLVDVGGGTGTMAKAIAAEFPDLECTVFDLPHVVAGLKGTKNVKYVAGNMFKEIPPADAYLLKWIIHDWSDEESLKILKRCKDGLREGKGRKLIIVDIVLGYCKEDAESVETQLFLDMLMIVLYNAKERTEREWAKLFVEAGFRDYKITPMLGLRSLIEVYP
>rna-XM_031534511.1
MEQILHAQAHIWNHCYSYISSMCLKCAIELGIPDTIHSHGGPMTLNQLVEAIPVRLGKAHCLYRVMRVLVYSGFFELQINKRGGAHEEEAAYSLNNISKLLIRDNPLSVEPYLRVMLNPIPMKSMDCLSAWFQNDDLCTVGTAHGMNPWDLVARNPMFGCLFNGAMASDSRLVARVLMDRCKWVFEGLKSLVDVGGGTGEMGKAIADALPHMEYTVLDLPHVVAGLQGTGNLKYVGGDMFNEKIPPADAILLKWILHDWTDEESVKILKKCKEAIPRKEEGGKLIIIEMVIDNPTEEDEKIKETQLLADVAMMNIMGLERNREEWAKLFTDAGFSGYKISPILGLRSVIEVYP
>rna-XM_031537139.1
MELTNGEKTSDLRECQAQVLVWNQILSYINSMSLKCAVELGIPDVIHNHGRPMTLPDLVSALGIPQARAGFLHRLMRMLSHSGFFALQTEQPCVNRDKGEEKPEGYVLTPPSQLLLKGDRSSKSMHPLVAFVVHPILIQPFNGLSKWFHSDSSGTPFEMQHGRAFWEYIARDPDFNLLTNEGMASDSSVVVSEIMDKCRRSFEQSTSVVDVGGGTGLLARKIADTFPHLEVTVLDLPHVVCGLEGTKTLKFLAGDMFDSIPPADIILLKWILHDWNDEEGVKILKRCKNAVTSNGKDGGKVIVIDVVLDDQEDEACSEMGLLFDMMMMATLTGRERCKKEWAKLFLDAGFRKYGITPLVGPRCLIEVYP
>rna-XM_031534443.1
MEDSGMSKTMGGNARGSLTHAQAHIWNHIFSFINSMSLKCALQLGIPDIIHNHGGPMTIGELVSALSVHSGKAHCIFHLMRILVHSGFFEQTSLRVVAEDDREEVGYALNNVSSLLLKDNPLSTRPFALAMLDPTLTDPWHCLGAWFLNDDPTPFSTEHGSLIWEHMGNEPEQNLRFNNAMASDARLVATEVLGSDCKRAFEGLRSLVDVGGGTGTMAKAIAAEFPDLECTVFDLPHVVAGLKGTKNVKYVAGNMFKEIPPADAYLLKWIIHDWSDEESLKILKRCKDGLREGKGRKLIIVDIVLGYCKEDAESVETQLFLDMLMIVLYNAKERTEREWAKLFVEAGFRDYKITPMLGLRSLIEVYP
>rna-XM_031534488.1
MGSFSSNDGAEKAMPVQLLQAQAHIWKHCYSYVSSMCLKCSLELGIPDAIQSHGGPMTFKQLVEAIPVCPGKARCLYRVMRVLVHWGFFELQIRKRGGAHEDEEAYSLNSASELLLRDNPVNLEPFVRFMLDPVSIKSMDCLSSWFQNDDLSPVETAHGLKAWDLVARNPELGCLFNGAMASDSRLVARVLTDHCKWAFEGLKSLVDVGGGTGETGKAIADAFPHMECTVFDLPHVVAGLQGTRNLKYVGGDMFNEKIPPADAILLKWILHDWTDEESVKILKKCKEAIPRKEEGGKLIIIETVMDDPTVGDENIKETQLLADVVMINVLGFERNKEEWAKLFAHAGFSDYVMRPILGLRSVIEVYP
>rna-XM_031534249.1
MDSSSSNNDGAEQVMDQLLQAQAHIWKHCYRYVSSRCLKCAIELGIPDAIHSHGGPMTFNQLVEAIPVRPGKAHCLYRVMRVLVHSGFFDLRIIKTGGAHEDEESYSLNSVSELLLRDNPVNIEPFVRFMLSPNLMKPLNCMSAWFQNDDLCTVETAHGMKPWDLMVRDPKFGSLFNSAMASDSQLVARMVTDCCKWVFEGLKSLVDIGGGTGEMGKVIADAFPQMEYTVFDLPHVVASLQGTRNLKYVGGDMFNEKIPPADAILLKWILHDWNDEESVQILKKCKEAIPIKEEGGKLIIIEMVMDNPTEGDEKIKETQLSADIFMMNAMGIERNREEWAKLFADAGFSSYKMSPILGLRSVIEVYP
MEGSGMNKTISGNARGSLVHAQTHIWNHIFSFVKSMSLKCAVQLGIPDIIHNHGGPMTINELVSALSVHPGKAHCISRLMRILVHSGFFEQTSLRVVAQDDREEVGYALNNVSSLLLKDNPLSTRPYALAMLDPALMNSGHCLGAWFLNDDPTPFSTEHGSLFFEHMGNGPEQNLLFNNAMASDARLLATEVLGSDCKRAFEGLRSLVDVGGGTGTMAKAIAAEFPDLECTVFDLPHVVAGLKGTKNVKYVGGNMFKEIPPADAYLLKWIIHDWSDEESVKILKRCKDGLREGKGCKLIIVDIVLGFGKEDEESVETQLFCDMQLMIILTGKERTEREWAKLFVEAGFRDYKITPMLGLRSLIEVYP
>rna-XM_031534050.1
MGSFSSNDGAKEAMQRLLQAQAHIWKHCYSYVSSMCLKCALELGIPEAIHSHSGPMTLNQLVEAIPVRPGKAHCLHRVMRVLVHSGFFELQIRKRGGAHEDEEVYSLNNESKLLLRDNPVNLDPFVLFTLDPITLKPMDCLSTWFQNDDLCTVETAHGMISWDIMVSNPKFGSLFNSAMASDSRLVARVVTDSCKWVFEGLKSLVDVGGGTGETGKAIADTFPDMEYTVFDLPHVVAGLQGTRNLKYVGGDMFNEKIPTADAVLLKWILHDWTDEESVQILKKCKEAIPRKEEGGKLIIIEMVMDNPAVGDEKTKETHLLADVFIMNIMGLERNTEEWAKLFADAGFSSYKISPVLGLRSVIEVYP
>rna-XM_031534421.1
MEDSGMSKTMGGNARGSLTHAQAHIWNHIFSFINSMSLKCALQLGIPDIIHNHGGPMTIGELVSALSVHSGKAHCIFHLMRILVHSGFFEQTSLRVVAEDDREEVGYALNNVSSLLLKDNPLSTRPFALAMLDPTLTDPWHCLGAWFLNDDPTPFSTEHGSLIWEHMGNEPEQNLRFNNAMASDARLVATEVLGSDCKRAFEGLRSLVDVGGGTGTMAKAIAAEFPDLECTVFDLPHVVAGLKGTKNVKYVAGNMFKEIPPADAYLLKWIIHDWSDEESLKILKRCKDGLREGKGRKLIIVDIVLGYCKEDAESVETQLFLDMLMIVLYNAKERTEREWAKLFVEAGFRDYKITPMLGLRSLIEVYP
>rna-XM_031534511.1
MEQILHAQAHIWNHCYSYISSMCLKCAIELGIPDTIHSHGGPMTLNQLVEAIPVRLGKAHCLYRVMRVLVYSGFFELQINKRGGAHEEEAAYSLNNISKLLIRDNPLSVEPYLRVMLNPIPMKSMDCLSAWFQNDDLCTVGTAHGMNPWDLVARNPMFGCLFNGAMASDSRLVARVLMDRCKWVFEGLKSLVDVGGGTGEMGKAIADALPHMEYTVLDLPHVVAGLQGTGNLKYVGGDMFNEKIPPADAILLKWILHDWTDEESVKILKKCKEAIPRKEEGGKLIIIEMVIDNPTEEDEKIKETQLLADVAMMNIMGLERNREEWAKLFTDAGFSGYKISPILGLRSVIEVYP
>rna-XM_031537139.1
MELTNGEKTSDLRECQAQVLVWNQILSYINSMSLKCAVELGIPDVIHNHGRPMTLPDLVSALGIPQARAGFLHRLMRMLSHSGFFALQTEQPCVNRDKGEEKPEGYVLTPPSQLLLKGDRSSKSMHPLVAFVVHPILIQPFNGLSKWFHSDSSGTPFEMQHGRAFWEYIARDPDFNLLTNEGMASDSSVVVSEIMDKCRRSFEQSTSVVDVGGGTGLLARKIADTFPHLEVTVLDLPHVVCGLEGTKTLKFLAGDMFDSIPPADIILLKWILHDWNDEEGVKILKRCKNAVTSNGKDGGKVIVIDVVLDDQEDEACSEMGLLFDMMMMATLTGRERCKKEWAKLFLDAGFRKYGITPLVGPRCLIEVYP
>rna-XM_031534443.1
MEDSGMSKTMGGNARGSLTHAQAHIWNHIFSFINSMSLKCALQLGIPDIIHNHGGPMTIGELVSALSVHSGKAHCIFHLMRILVHSGFFEQTSLRVVAEDDREEVGYALNNVSSLLLKDNPLSTRPFALAMLDPTLTDPWHCLGAWFLNDDPTPFSTEHGSLIWEHMGNEPEQNLRFNNAMASDARLVATEVLGSDCKRAFEGLRSLVDVGGGTGTMAKAIAAEFPDLECTVFDLPHVVAGLKGTKNVKYVAGNMFKEIPPADAYLLKWIIHDWSDEESLKILKRCKDGLREGKGRKLIIVDIVLGYCKEDAESVETQLFLDMLMIVLYNAKERTEREWAKLFVEAGFRDYKITPMLGLRSLIEVYP
>rna-XM_031534488.1
MGSFSSNDGAEKAMPVQLLQAQAHIWKHCYSYVSSMCLKCSLELGIPDAIQSHGGPMTFKQLVEAIPVCPGKARCLYRVMRVLVHWGFFELQIRKRGGAHEDEEAYSLNSASELLLRDNPVNLEPFVRFMLDPVSIKSMDCLSSWFQNDDLSPVETAHGLKAWDLVARNPELGCLFNGAMASDSRLVARVLTDHCKWAFEGLKSLVDVGGGTGETGKAIADAFPHMECTVFDLPHVVAGLQGTRNLKYVGGDMFNEKIPPADAILLKWILHDWTDEESVKILKKCKEAIPRKEEGGKLIIIETVMDDPTVGDENIKETQLLADVVMINVLGFERNKEEWAKLFAHAGFSDYVMRPILGLRSVIEVYP
>rna-XM_031534249.1
MDSSSSNNDGAEQVMDQLLQAQAHIWKHCYRYVSSRCLKCAIELGIPDAIHSHGGPMTFNQLVEAIPVRPGKAHCLYRVMRVLVHSGFFDLRIIKTGGAHEDEESYSLNSVSELLLRDNPVNIEPFVRFMLSPNLMKPLNCMSAWFQNDDLCTVETAHGMKPWDLMVRDPKFGSLFNSAMASDSQLVARMVTDCCKWVFEGLKSLVDIGGGTGEMGKVIADAFPQMEYTVFDLPHVVASLQGTRNLKYVGGDMFNEKIPPADAILLKWILHDWNDEESVQILKKCKEAIPIKEEGGKLIIIEMVMDNPTEGDEKIKETQLSADIFMMNAMGIERNREEWAKLFADAGFSSYKMSPILGLRSVIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University