Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product

Species information
Populus alba
Species name
White poplar (Populus alba) is a species of eudicot in the family Salicaceae (willow family).
Species info

Gene sequence info
Populus_alba.FLS.fa
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>rna-XM_035049249.1
METERVQNIASTFEDTIPEAFIRSEHEQPAITTVHGVNLDVPVIDVSDPDEEKITRLIADASREWGMFQIVNHGIPSEVISKFQSVGRAFFELPQVEKELYAKPPGAKSIEGYGTFLQKEVEGKKGWVDHLFHRIWPPPAINYRFWPKNPPLYREANEEYVKYLHGVVDKLFKSLSLDLGLEEHELKEAVGGDELTSLKINYYPPPRPDLALGLVAHTDMCSITILLPNDIQGLQACRDGQWYCVKYIPNALVIHIGDQIEILSNGKYKSVFHRTTVTKDKTRMSWPVFLEPPPDLAVGPHPSSLMNRIPPVQTKKYGDYCYCKLNKIPQ
>rna-XM_035061266.1
MHARNSSLSLYIEGSLLCFEEKLRNKKITFRMEFDRVQAIASLSFDKQAIPEVIRPEKDQPDRVQTIASLSFDKEAIPQEFIRPEKEQPAITTFRGLAPEIPAIDLSEPDQEKLVGLIADASKEWGIFQVINHGIPSDLIAELQGVGQKFFELPQEEKEVCARPRDSKSIEGYGSKLQKDPQEKKSWVDHLFHRIWPPPSINYQFWPENPPSYREVTKEYAKHMRDVVDKLFTTLSLGLGLEGHVLKEAAGGEQIEYMLKINYYPPCPRPDLTLGVVAHTDLSAITILVPNEVPGLQIFKDDQWIEAKYIPDALIIHIGDQIEILSNGKYKAVLHRTTVAKDRARMSWPVFLEPPGELVVGPLPQFINKDNPPKFKTKKFKDYTYCKLNKLPQ
>rna-XM_035061243.1
MEFDRVQTIASLSFDKEAIPEEFIMPEKEQPATTTFRGVAPEIPAIDLSEPDQEKLVGLIADASKEWGIFQVINHGIPSDLIAELQGVGKRFFELPQEEKEVCARPRDSESIEGYGSKLLNDPQEKKTWVDHLFHRIWPPPSINYQFWPENPPYSREVNKEYAKYTRNVVDKLFTTLSLGLGLEGHVLKEAAGGEQIEYMLKINYYPPCPRPDLTLGVEAHTDLSAITILVPNEVPGLQIFKDDHWIEAKYIPDALIIHIGDQIEILSNGKYKAVLHRTTVAKDRARMSWPVFLEPPGELVVGPLPQFINKDNPTKFKTKKFKDYMHCKLNKLPQ
>rna-XM_035062732.1
MEFDRVQAIASLSFDKETIPEEFIIRPEKEQPATTTFHGAVPEIPTIDISDPNQENLVRLVADASKEWGIFQVVNHGIPSDLIAKLQDVGKKFFELPQEEKEVYAKPHDSTSIEGYGSKLQNNPQENKSWVDHLFHRIWPPSSINHQFWPQNPPSYREVNKEYAKYMREVTDKLFTALSLGLGLEGHALKEGAGGEDIEYMLKINYYPPCPRPDLTLGVAAHTDLSALTILVPNEVPGLQIFKDGNWFEAKYIPNALIIHIGDQIEILSNGKYKAVLHRTTVAKDKARMSWPVFLEPPGELVVCPLPQLINKDNPPKFKAKKFKDYMYCKLNHLPQ
>rna-XM_035071075.1
MEFDRVQAIASLSFDKETIPEEFIIRPEKEQPATTTFHGAVPEIPTIDISDPNQETLVRLIADASKEWGIFQVVSHGIPGDLIAKLQDVGKKFFELPQEEKEVYAKPRDSTNIEGYGSKLQNNPQENKSWVDHLFHRIWPPSSINHQFWPQNPPSYREVNEEYAKHMREVTDKLFTALSLGLGLEGHALKEGAGGEDIEYMLKINYYPPCPRPDLTLGVAAHTDLSALTILVPNEVPGLQIFKDGNWFEAKYIPNALIIHIGDQIEILSNGKYKAVLHRTTVAKDKARMSWPVFLEPPGELVVGPLPHLINKDNPPKFMAKKFKDYMYCKLNHLPQ
>rna-XM_035076484.1
MAISPESMPENPIDFRAPPPSPIASGRRSSVTNDEVLTEFLEHSLRVPDLILPDKIFPRQKIVETPPRIDCQSLISGESDSVLRMLDSIARIGCFQLVNFGIPSEFIRLVSVTAAGIFQLPPEKKEAVSRSLERPYGFEEVHGDHQEAESEVTEEFVWCKDESLKLDMEGIWPTGYSNFSKKMETLSSDIEKVARKILQILHENCPRKSMNGNDMMQRQDLIGSVCCLYKHGRNFLADQWAGSLGYDVMRMLIRGTDYSHALCLHICDGSSEFHVYSKKGWVSFCPDKDALIVTVGDRAQVWSGGQYKHVFGWPIFKGEDQDSISMAFLYSPPSSNSSSSKTSKGKKTVSLGQQAIVAVILTLVCHFLVYFYKEV
>rna-XM_035036195.1
MAVSPEYMPENPIDFRAPPPSPIASGRRSSVTNDGVLSEFLEHSLRVPDLFLPDNFFPKQKIVETPPRIDCQSLNSKESNSVLKTLDSIARIGCFQLVNFGIPSEFIKLVSVMAAGIFQLPSEKKAAVMKSPEKPYGFEEVHGDQEAENELSEEFVWSKDESLKLDMERIWPTGYSNFSEKMETLSSDLGNLAAKILQVLRENCPRKSKYANDMMQRQDLTGPVCCLYKHRRDIPADRLASSLGYDVFSMLIRGNDYSHALCLHICDGSSQFHVYSKKGWVSFCPDKDALIVTVGDQTQAWSGGQYKQVLGRPIFKGEDEDNISMAFLYSPPNTIICCSKTTKGKALSLGQQAILAIILTLVYHLLVYFYKKL
>rna-XM_035037227.1
MAAKAELISNSVQEMALNGKEPPVKYFCKGNDVGVLDASVPLIDIPVVDLGLLTSPSTSAQELEKFHLAVSSRGCFQVVNHGMTSSFLDKIRDVSKRFFALSMEDKQKYSREADSIEGYGNDMILSDHQTIDWSDRLYLTISPEDQRKIKFWPENPKDFRETLNEYTMKLQEINEILLRAMAMSLNLEESSFLDQYGERPLVAARFNFYPPCPRPDRILGVKPHADASAITFLLQDKEVEGLQFLKDNQRFRVPIIPQALLINVGDQVEIMSNGIFKSPDNEHRDLFMYGEITKCA
METERVQNIASTFEDTIPEAFIRSEHEQPAITTVHGVNLDVPVIDVSDPDEEKITRLIADASREWGMFQIVNHGIPSEVISKFQSVGRAFFELPQVEKELYAKPPGAKSIEGYGTFLQKEVEGKKGWVDHLFHRIWPPPAINYRFWPKNPPLYREANEEYVKYLHGVVDKLFKSLSLDLGLEEHELKEAVGGDELTSLKINYYPPPRPDLALGLVAHTDMCSITILLPNDIQGLQACRDGQWYCVKYIPNALVIHIGDQIEILSNGKYKSVFHRTTVTKDKTRMSWPVFLEPPPDLAVGPHPSSLMNRIPPVQTKKYGDYCYCKLNKIPQ
>rna-XM_035061266.1
MHARNSSLSLYIEGSLLCFEEKLRNKKITFRMEFDRVQAIASLSFDKQAIPEVIRPEKDQPDRVQTIASLSFDKEAIPQEFIRPEKEQPAITTFRGLAPEIPAIDLSEPDQEKLVGLIADASKEWGIFQVINHGIPSDLIAELQGVGQKFFELPQEEKEVCARPRDSKSIEGYGSKLQKDPQEKKSWVDHLFHRIWPPPSINYQFWPENPPSYREVTKEYAKHMRDVVDKLFTTLSLGLGLEGHVLKEAAGGEQIEYMLKINYYPPCPRPDLTLGVVAHTDLSAITILVPNEVPGLQIFKDDQWIEAKYIPDALIIHIGDQIEILSNGKYKAVLHRTTVAKDRARMSWPVFLEPPGELVVGPLPQFINKDNPPKFKTKKFKDYTYCKLNKLPQ
>rna-XM_035061243.1
MEFDRVQTIASLSFDKEAIPEEFIMPEKEQPATTTFRGVAPEIPAIDLSEPDQEKLVGLIADASKEWGIFQVINHGIPSDLIAELQGVGKRFFELPQEEKEVCARPRDSESIEGYGSKLLNDPQEKKTWVDHLFHRIWPPPSINYQFWPENPPYSREVNKEYAKYTRNVVDKLFTTLSLGLGLEGHVLKEAAGGEQIEYMLKINYYPPCPRPDLTLGVEAHTDLSAITILVPNEVPGLQIFKDDHWIEAKYIPDALIIHIGDQIEILSNGKYKAVLHRTTVAKDRARMSWPVFLEPPGELVVGPLPQFINKDNPTKFKTKKFKDYMHCKLNKLPQ
>rna-XM_035062732.1
MEFDRVQAIASLSFDKETIPEEFIIRPEKEQPATTTFHGAVPEIPTIDISDPNQENLVRLVADASKEWGIFQVVNHGIPSDLIAKLQDVGKKFFELPQEEKEVYAKPHDSTSIEGYGSKLQNNPQENKSWVDHLFHRIWPPSSINHQFWPQNPPSYREVNKEYAKYMREVTDKLFTALSLGLGLEGHALKEGAGGEDIEYMLKINYYPPCPRPDLTLGVAAHTDLSALTILVPNEVPGLQIFKDGNWFEAKYIPNALIIHIGDQIEILSNGKYKAVLHRTTVAKDKARMSWPVFLEPPGELVVCPLPQLINKDNPPKFKAKKFKDYMYCKLNHLPQ
>rna-XM_035071075.1
MEFDRVQAIASLSFDKETIPEEFIIRPEKEQPATTTFHGAVPEIPTIDISDPNQETLVRLIADASKEWGIFQVVSHGIPGDLIAKLQDVGKKFFELPQEEKEVYAKPRDSTNIEGYGSKLQNNPQENKSWVDHLFHRIWPPSSINHQFWPQNPPSYREVNEEYAKHMREVTDKLFTALSLGLGLEGHALKEGAGGEDIEYMLKINYYPPCPRPDLTLGVAAHTDLSALTILVPNEVPGLQIFKDGNWFEAKYIPNALIIHIGDQIEILSNGKYKAVLHRTTVAKDKARMSWPVFLEPPGELVVGPLPHLINKDNPPKFMAKKFKDYMYCKLNHLPQ
>rna-XM_035076484.1
MAISPESMPENPIDFRAPPPSPIASGRRSSVTNDEVLTEFLEHSLRVPDLILPDKIFPRQKIVETPPRIDCQSLISGESDSVLRMLDSIARIGCFQLVNFGIPSEFIRLVSVTAAGIFQLPPEKKEAVSRSLERPYGFEEVHGDHQEAESEVTEEFVWCKDESLKLDMEGIWPTGYSNFSKKMETLSSDIEKVARKILQILHENCPRKSMNGNDMMQRQDLIGSVCCLYKHGRNFLADQWAGSLGYDVMRMLIRGTDYSHALCLHICDGSSEFHVYSKKGWVSFCPDKDALIVTVGDRAQVWSGGQYKHVFGWPIFKGEDQDSISMAFLYSPPSSNSSSSKTSKGKKTVSLGQQAIVAVILTLVCHFLVYFYKEV
>rna-XM_035036195.1
MAVSPEYMPENPIDFRAPPPSPIASGRRSSVTNDGVLSEFLEHSLRVPDLFLPDNFFPKQKIVETPPRIDCQSLNSKESNSVLKTLDSIARIGCFQLVNFGIPSEFIKLVSVMAAGIFQLPSEKKAAVMKSPEKPYGFEEVHGDQEAENELSEEFVWSKDESLKLDMERIWPTGYSNFSEKMETLSSDLGNLAAKILQVLRENCPRKSKYANDMMQRQDLTGPVCCLYKHRRDIPADRLASSLGYDVFSMLIRGNDYSHALCLHICDGSSQFHVYSKKGWVSFCPDKDALIVTVGDQTQAWSGGQYKQVLGRPIFKGEDEDNISMAFLYSPPNTIICCSKTTKGKALSLGQQAILAIILTLVYHLLVYFYKKL
>rna-XM_035037227.1
MAAKAELISNSVQEMALNGKEPPVKYFCKGNDVGVLDASVPLIDIPVVDLGLLTSPSTSAQELEKFHLAVSSRGCFQVVNHGMTSSFLDKIRDVSKRFFALSMEDKQKYSREADSIEGYGNDMILSDHQTIDWSDRLYLTISPEDQRKIKFWPENPKDFRETLNEYTMKLQEINEILLRAMAMSLNLEESSFLDQYGERPLVAARFNFYPPCPRPDRILGVKPHADASAITFLLQDKEVEGLQFLKDNQRFRVPIIPQALLINVGDQVEIMSNGIFKSPDNEHRDLFMYGEITKCA
Gene tree
Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University