Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Poncirus trifoliata
Species name
Hardy orange (Citrus trifoliata) is a species of eudicot in the family Rutaceae (rue family).
Species info
Gene sequence info
Poncirus_trifoliata.HI4_OMT.fa
Download
>Ptrif.0002s2408.1.v1.3.1
MDPIQNEGAKELFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVQSAQQQQEEAYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYVAKNPDFNNIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPAIKCTVLDLPHVVPKVPDTDNLTFIAGDMFQSIPPADAFFFKAIFHAFVDKDCLKVLKRCREPIASRGDRGKVIIIDIVINEKKEDAGSPKQSYCTTC
>Ptrif.0002s2820.1.v1.3.1
MSLKCAVELGIADVIHSHGRPITLSELASALNIQPTKTRSLFHFMRLFVHVGLFSKTKVVNHNEQEEAYGLTPTSTLLIKDNSYCLSPLVTGLLDQDFVSSFQYLSSWFKANDLTLWETVRGLKFWDYLNQNPVLSQQFNQVIESDSVMATLLVKDCRPIFQGLRSMVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVEGDMFQFIPPADAFFFKTVFHFFDVEDCLKLLKKCREAIASNGERGKHYNRIGFSWHGKYAVNGI
>Ptrif.0002s2822.1.v1.3.1
MGDIENDRVTVRELFEGQAHLYKGIVKNLSSMSLKCAVELGIADAIHSHGGPITLSELASALNIQPTKTRSLFHFMRLLVHMGLFSKTKVDNHNEQEEAYGLTPTSTLLIKDKPYCLSPLVTGLLDQDYVSSFQHLSRWFKGNDLTLWESFRGLKFWDYLNQNTPLTKRFNQAMTSDSEMATLIVKDCKPIFQGLRSLVDIGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVEGDMFQFIPPADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDSELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAGFTHYKVAPIFGIKSLIEVYP
>Ptrif.0002s2409.1.v1.3.1
MDPIQNEGAKEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLSKLMRLLVHTGFFSKANVQSVQQQQEEAYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYVAKNPDFNNIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPAIKCTVLDLPHVVPKVPDTDNLTFIAGDMFQSIPPADAFFFKAIFHAFVDEDSLKILKRCREAIASRGDRGKVIIIDIVINEKKEDAQLTEAKLLYDMLMMVAVRGSERTEKEWEKLFLDAGFSHFKITPVYGIKSLIEVYP
>Ptrif.0005s1389.1.v1.3.1
MELIQDEGAKELLHANSHLFKLIYNYVSSMSLRCAVELDIPEIIHKHGRPITLSELVSALEIHPPKVHGLFRLMRLLVHSGFFSTKKVQGEEAYILTSSSKMFLKEKSYCYTPLVLALTDPVFVNPCHFLSHWFRGNNDLATFQTAHDGLNFWDYAEKNPEFSNLFNEAMACDSQMANLIVKDCKPIFQGLSSLVDVGGGTGSFARIISEAFPGIKCTVLDRAHVVPNLPDNENLKFIAGDMFQFIPPADAFFFKLIFHAFVDEDCLKILKKCREAIASKGDEGGKLIIIDIVINEKKDEAEVTEAKLLYDMLMMVAVRGIERTEKEWKRLFLDAGFTRYKITPLFGIKSVIEVFP
>Ptrif.0005s0377.1.v1.3.1
MSSIEVEDANELLQAQAHLYKHVLSYMSSIYLKCAVELGIPDIIHKRGRPVTLPELVSALEFQPNKRNCLRRIMRLLDHSGFFSTTKVHNSREEENEAYALTFASKLLLKDKSYCLSPFVLLVTDAAFITPGHYLSRWLRGNELPDPFVTGHGINIWGYAEQNHEFNNLFNQGLASDSQMAKLIIKDCKHIFDGLSSLVEVGGGTGSFARIISEAFPSIKCSVLELPHVIADLPETDNLKFIAGDMNQSIPFADAFLFKLIFHDYDDEVCLKLLKNCREAMASSDAREKVIIVDIVVNEKKDEPEITEAKLLYDALMMTCVPGIERSEKEWERLFLDAGFTSYKITPLLGLRSFIEVYL
>Ptrif.0009s1128.1.v1.3.1
MDANQDQGAKELFQGQARLYKLMFNHLSSMSLKCAIELGIADIIHSHGRAITLSELVSALDIQPTKTSGLFRLMRLLVHSGCFNKTKVNGQEEAYGLTAASTLLIKEKPYCFSPTVSLFLDPCFVAAFQSLSSWFKGTEFTLWETVHGIKFWEFMNQNPGINQRFNEATASDTEIMTSFVVKAECKQIFEGLGSLVDVGGGNGSLSRIISEAFPGIKCTVLDLPHVVANLPEADNLKFIAGDMFQFIPLADAFLFKLVFHGLGDEDGLKILKKRREAIASNGERGKVIIMDIVINAEEEEHALTETKFLFDISMSVNANGKERTDSEWAKLFSDAGFSHYKITPIFGMRSLIEVYP
>Ptrif.0009s1249.1.v1.3.1
MDANQDQGAKELFQGQAQLYKLMFNHLSSMSLKCAIELGIADIIHSHGRPITLSELVSALDIQPTKTTGLFRLMCLLVHSGCFKKTKVNGQEEAYGLTAASTLLIKDKPCCLSPTVSVFVDPFFVAPFQSLSSWFKGTELTLWETVHGIKFWEFMNQNPAINQRFNEAMASDSEIMTSFVVKSECKQIFERLGSLVDVGGGNGSFSRIISEAFPGIKCTVLDLPHVVANLPEADNLKYIAGDMFRFIPPADAFLFKLVFHGLGDEDGLKILKKRREAIASNGERGKVIIIDIVINAEEEEHELTETKFLFDIMMSVNATGKERTESEWAKLFFDAGFSHYKITPIFGMKSLIEVYA
MDPIQNEGAKELFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVQSAQQQQEEAYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYVAKNPDFNNIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPAIKCTVLDLPHVVPKVPDTDNLTFIAGDMFQSIPPADAFFFKAIFHAFVDKDCLKVLKRCREPIASRGDRGKVIIIDIVINEKKEDAGSPKQSYCTTC
>Ptrif.0002s2820.1.v1.3.1
MSLKCAVELGIADVIHSHGRPITLSELASALNIQPTKTRSLFHFMRLFVHVGLFSKTKVVNHNEQEEAYGLTPTSTLLIKDNSYCLSPLVTGLLDQDFVSSFQYLSSWFKANDLTLWETVRGLKFWDYLNQNPVLSQQFNQVIESDSVMATLLVKDCRPIFQGLRSMVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVEGDMFQFIPPADAFFFKTVFHFFDVEDCLKLLKKCREAIASNGERGKHYNRIGFSWHGKYAVNGI
>Ptrif.0002s2822.1.v1.3.1
MGDIENDRVTVRELFEGQAHLYKGIVKNLSSMSLKCAVELGIADAIHSHGGPITLSELASALNIQPTKTRSLFHFMRLLVHMGLFSKTKVDNHNEQEEAYGLTPTSTLLIKDKPYCLSPLVTGLLDQDYVSSFQHLSRWFKGNDLTLWESFRGLKFWDYLNQNTPLTKRFNQAMTSDSEMATLIVKDCKPIFQGLRSLVDIGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVEGDMFQFIPPADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDSELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAGFTHYKVAPIFGIKSLIEVYP
>Ptrif.0002s2409.1.v1.3.1
MDPIQNEGAKEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLSKLMRLLVHTGFFSKANVQSVQQQQEEAYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYVAKNPDFNNIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPAIKCTVLDLPHVVPKVPDTDNLTFIAGDMFQSIPPADAFFFKAIFHAFVDEDSLKILKRCREAIASRGDRGKVIIIDIVINEKKEDAQLTEAKLLYDMLMMVAVRGSERTEKEWEKLFLDAGFSHFKITPVYGIKSLIEVYP
>Ptrif.0005s1389.1.v1.3.1
MELIQDEGAKELLHANSHLFKLIYNYVSSMSLRCAVELDIPEIIHKHGRPITLSELVSALEIHPPKVHGLFRLMRLLVHSGFFSTKKVQGEEAYILTSSSKMFLKEKSYCYTPLVLALTDPVFVNPCHFLSHWFRGNNDLATFQTAHDGLNFWDYAEKNPEFSNLFNEAMACDSQMANLIVKDCKPIFQGLSSLVDVGGGTGSFARIISEAFPGIKCTVLDRAHVVPNLPDNENLKFIAGDMFQFIPPADAFFFKLIFHAFVDEDCLKILKKCREAIASKGDEGGKLIIIDIVINEKKDEAEVTEAKLLYDMLMMVAVRGIERTEKEWKRLFLDAGFTRYKITPLFGIKSVIEVFP
>Ptrif.0005s0377.1.v1.3.1
MSSIEVEDANELLQAQAHLYKHVLSYMSSIYLKCAVELGIPDIIHKRGRPVTLPELVSALEFQPNKRNCLRRIMRLLDHSGFFSTTKVHNSREEENEAYALTFASKLLLKDKSYCLSPFVLLVTDAAFITPGHYLSRWLRGNELPDPFVTGHGINIWGYAEQNHEFNNLFNQGLASDSQMAKLIIKDCKHIFDGLSSLVEVGGGTGSFARIISEAFPSIKCSVLELPHVIADLPETDNLKFIAGDMNQSIPFADAFLFKLIFHDYDDEVCLKLLKNCREAMASSDAREKVIIVDIVVNEKKDEPEITEAKLLYDALMMTCVPGIERSEKEWERLFLDAGFTSYKITPLLGLRSFIEVYL
>Ptrif.0009s1128.1.v1.3.1
MDANQDQGAKELFQGQARLYKLMFNHLSSMSLKCAIELGIADIIHSHGRAITLSELVSALDIQPTKTSGLFRLMRLLVHSGCFNKTKVNGQEEAYGLTAASTLLIKEKPYCFSPTVSLFLDPCFVAAFQSLSSWFKGTEFTLWETVHGIKFWEFMNQNPGINQRFNEATASDTEIMTSFVVKAECKQIFEGLGSLVDVGGGNGSLSRIISEAFPGIKCTVLDLPHVVANLPEADNLKFIAGDMFQFIPLADAFLFKLVFHGLGDEDGLKILKKRREAIASNGERGKVIIMDIVINAEEEEHALTETKFLFDISMSVNANGKERTDSEWAKLFSDAGFSHYKITPIFGMRSLIEVYP
>Ptrif.0009s1249.1.v1.3.1
MDANQDQGAKELFQGQAQLYKLMFNHLSSMSLKCAIELGIADIIHSHGRPITLSELVSALDIQPTKTTGLFRLMCLLVHSGCFKKTKVNGQEEAYGLTAASTLLIKDKPCCLSPTVSVFVDPFFVAPFQSLSSWFKGTELTLWETVHGIKFWEFMNQNPAINQRFNEAMASDSEIMTSFVVKSECKQIFERLGSLVDVGGGNGSFSRIISEAFPGIKCTVLDLPHVVANLPEADNLKYIAGDMFRFIPPADAFLFKLVFHGLGDEDGLKILKKRREAIASNGERGKVIIIDIVINAEEEEHELTETKFLFDIMMSVNATGKERTESEWAKLFFDAGFSHYKITPIFGMKSLIEVYA
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University