Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Poncirus trifoliata
Species name
Hardy orange (Citrus trifoliata) is a species of eudicot in the family Rutaceae (rue family).
Species info
Gene sequence info
Poncirus_trifoliata.ROMT.fa
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>Ptrif.0001s1951.1.v1.3.1
MFEAIPPADAVLIKCVLHNWNDEECVKILNNCKKAIAINGKAGNVIIIDIRMESEKADYKTTETQLFMDMLMMVLVKGEERNEKEWARLFFEAGFSDYKITDVLGVRSLIEVYP
>Ptrif.0001s1949.1.v1.3.1
MDLTKGKNNDNISDELLQSQAHVWNNIFSFVNSMSLKCAIQLGIPNIIHNHAKPMTINQLLTALQIHPTKTQCVYHLMRILVHSGFFALQKASENEQEEGYILTSASKLLLKDHPLSLVPFLLAMLDPILTKPWHQVVRETRLNHFFNEGMASDTRLTSSALIHKCKDVFEGLNTLVDFPHIECIVFDQPHVVADLKSNGNLKYVGGNMFEAIPPADAVLIKNVLRF
>Ptrif.0001s1950.1.v1.3.1
MDLTKGKNNDNISDELLQSQAHVWNNIFSFVNSMSLKCAIQLGIPNIIHNHAKPMTINQLLTALQIHPTKTQCVYHLMRILVHSGFFALQKASENEQEEGYILTSASKLLLKDHPLSLVPFLLAMLDPILTKPWHQVVRETRLNHFFNEGMASDTRLTSSALIHKCKDVFEGLNTLVDRHSQKFPHIECIVFDQPHVVADLKSNGNLKYVGGNMFEAIPPADAVLIKILKNCKKAIAINGKAGKVIIIDIRMESEKADYKTTETQLFMDMLMMVLVKGEERNEKEWARLFFEAGFSDYKITDVLGVRSLIEVYP
>Ptrif.0002s2408.1.v1.3.1
MDPIQNEGAKELFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVQSAQQQQEEAYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYVAKNPDFNNIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPAIKCTVLDLPHVVPKVPDTDNLTFIAGDMFQSIPPADAFFFKAIFHAFVDKDCLKVLKRCREPIASRGDRGKVIIIDIVINEKKEDAGSPKQSYCTTC
>Ptrif.0002s0812.1.v1.3.1
MDMNHMINAESSSSSSSSELLKAQAQVWNCAFNYINSMSLKCAVELGIPDVIHKHGQPMTLSQIASALDIQKNKAHCIQRLMRILAHSGFFAQQNKIDKDESEVYYSLTPASRLLLKDDPLRAAPFMFLITHPIVTTPFNFLSSWFKNDDPSTPTVFESTHGKDIWGCLAQEAKFKNVFYDAMMTDSQFISSVLIKDCKEVFKGLKCLVDLGGGTGTMAKAIAEAFPEMKCIVFDLPHVVDNNLQQQQGANKNLEFLGGDMFEAIPPADAVLLKWVLHNWGDEESVKILKKCKEAIDGSKDKGGKVIVIDMVIEDQSIDKDSTETQLCCDMLMMSLFSVAKERSIKEWKKLFLEAGFSHYNVNSTLGLRSLIELYP
>Ptrif.0002s0813.1.v1.3.1
MNLIDGEHDTELLEAQAHVWNHIFNFINSMSLKCAVELGIPDIINKHGKPTTLNELVSALTINPSKTRCVYRLMRILIHSGFFAQQTLNSSRNNNDEEQGYVLTNASKLLLKDNPLSVTPFLQAMLDPILLSPWLKLSTWFQNDDPTPFDTAHGKSFWEYAGDEPKINNFFNEAMASDARLATRVVIHKCKDVFEGLNSLVDVGGGTGTVAKAIAKAFPNLECTDFDLPHVVNGLESDLANLKYVGGDMFEAIPPADAVLFKWILHDWNDEECVKILKKCKEAITSDGKKGKVIIIDMMRENKKRDSESIETQLFFDMLMMVLVTGTERDEKEWAKLFADAGFSDYKITPILGLRSLIEVYP
>Ptrif.0002s2820.1.v1.3.1
MSLKCAVELGIADVIHSHGRPITLSELASALNIQPTKTRSLFHFMRLFVHVGLFSKTKVVNHNEQEEAYGLTPTSTLLIKDNSYCLSPLVTGLLDQDFVSSFQYLSSWFKANDLTLWETVRGLKFWDYLNQNPVLSQQFNQVIESDSVMATLLVKDCRPIFQGLRSMVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVEGDMFQFIPPADAFFFKTVFHFFDVEDCLKLLKKCREAIASNGERGKHYNRIGFSWHGKYAVNGI
>Ptrif.0002s2822.1.v1.3.1
MGDIENDRVTVRELFEGQAHLYKGIVKNLSSMSLKCAVELGIADAIHSHGGPITLSELASALNIQPTKTRSLFHFMRLLVHMGLFSKTKVDNHNEQEEAYGLTPTSTLLIKDKPYCLSPLVTGLLDQDYVSSFQHLSRWFKGNDLTLWESFRGLKFWDYLNQNTPLTKRFNQAMTSDSEMATLIVKDCKPIFQGLRSLVDIGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVEGDMFQFIPPADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDSELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAGFTHYKVAPIFGIKSLIEVYP
>Ptrif.0002s3316.1.v1.3.1
MINGENAASSSELLIAQAQVWNCTFNYVNSMSLKCAVELGIADVIHSHGQPTTLSQIASALDIKPNKVECFQSVMRTLVHSGFFAQQKDDEYFLTPASRLLLKDTPLKAAPFVDLVADPLYTTAFHCLGTWLQNDDPTLFETAHGKKVWDRLIAGIVIKDCKEVFEGLKSLIDVAGGTGIMARAIATAFPDIKCTVFDLPHVVDNLQGTNDNLDFLGGNMFEAIPQANAVLLKWILHNWNDEESVKLLKKCKEAIPSKDEGGKVIIIDMAIENQSQDKESMETQLCFDILMVSLFRGKERSVDDWKKLFLAAGFSHYKLTPILGVRSLIEAYP
>Ptrif.0002s2409.1.v1.3.1
MDPIQNEGAKEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLSKLMRLLVHTGFFSKANVQSVQQQQEEAYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYVAKNPDFNNIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPAIKCTVLDLPHVVPKVPDTDNLTFIAGDMFQSIPPADAFFFKAIFHAFVDEDSLKILKRCREAIASRGDRGKVIIIDIVINEKKEDAQLTEAKLLYDMLMMVAVRGSERTEKEWEKLFLDAGFSHFKITPVYGIKSLIEVYP
>Ptrif.0005s3043.1.v1.3.1
MIADFELITSIVVEDCKEVFKGLKSLVDVGGGTRTMARAIATGFLDIKCIVLICLLLWGTNKLEFF
>Ptrif.0005s0416.1.v1.3.1
MSLPMCLCDVLACVERDYAHGHITWLQNDDQTLFGTADGKNFWDYIADEVNFKSIFYEAMITDFELIASVLIEDCTEVTGTMARAIATAFPDTKCTVFDLPLVVDNLVGINNLDFFGGNMFEAIPTPNAVLLKIFNNYSNLKKLTNQLTWWILHDWSDEESLKILKKCKESIPSTERKSKETMETQLCFDMLVATFLKGKERSVYYWKKLFLAAGFSHYKITPNLGLWSLIEAYP
>Ptrif.0006s0175.1.v1.3.1
MDLIDGDHDTELLEAQTHVWNHIFNFINSMTLKCAIQLGIPDIISKHGKPMTLNELVSALTINPSKSRCVYRLMRILIHTSFFAQQKLISSRNNNDEEQGYVLTNASKLLLKDNPLSVTPFLQTMLDPILLSPWQQLSTWFQNDDPTPFDTAHGKTLWEYAGDEPKLNNFFNEAMASDARLATSVMIHKCKDVFEGLNSLVDVGGGIGTAAKVIAKAFPKLECTCFDLPHVVNGLDSDLANLKYVGGDMFEAISPADAVLLKWILHDWNDEECVKILKKCKEAITSDGKKGKVIIIDMIRENKKGDDKSIETQLFFDMLMMVLLTGTERDEKEWAKLFTYAGFSDYKIIPILGLRSLIEVYP
MFEAIPPADAVLIKCVLHNWNDEECVKILNNCKKAIAINGKAGNVIIIDIRMESEKADYKTTETQLFMDMLMMVLVKGEERNEKEWARLFFEAGFSDYKITDVLGVRSLIEVYP
>Ptrif.0001s1949.1.v1.3.1
MDLTKGKNNDNISDELLQSQAHVWNNIFSFVNSMSLKCAIQLGIPNIIHNHAKPMTINQLLTALQIHPTKTQCVYHLMRILVHSGFFALQKASENEQEEGYILTSASKLLLKDHPLSLVPFLLAMLDPILTKPWHQVVRETRLNHFFNEGMASDTRLTSSALIHKCKDVFEGLNTLVDFPHIECIVFDQPHVVADLKSNGNLKYVGGNMFEAIPPADAVLIKNVLRF
>Ptrif.0001s1950.1.v1.3.1
MDLTKGKNNDNISDELLQSQAHVWNNIFSFVNSMSLKCAIQLGIPNIIHNHAKPMTINQLLTALQIHPTKTQCVYHLMRILVHSGFFALQKASENEQEEGYILTSASKLLLKDHPLSLVPFLLAMLDPILTKPWHQVVRETRLNHFFNEGMASDTRLTSSALIHKCKDVFEGLNTLVDRHSQKFPHIECIVFDQPHVVADLKSNGNLKYVGGNMFEAIPPADAVLIKILKNCKKAIAINGKAGKVIIIDIRMESEKADYKTTETQLFMDMLMMVLVKGEERNEKEWARLFFEAGFSDYKITDVLGVRSLIEVYP
>Ptrif.0002s2408.1.v1.3.1
MDPIQNEGAKELFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLFKLMRLLVHTGFFSTANVQSAQQQQEEAYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYVAKNPDFNNIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPAIKCTVLDLPHVVPKVPDTDNLTFIAGDMFQSIPPADAFFFKAIFHAFVDKDCLKVLKRCREPIASRGDRGKVIIIDIVINEKKEDAGSPKQSYCTTC
>Ptrif.0002s0812.1.v1.3.1
MDMNHMINAESSSSSSSSELLKAQAQVWNCAFNYINSMSLKCAVELGIPDVIHKHGQPMTLSQIASALDIQKNKAHCIQRLMRILAHSGFFAQQNKIDKDESEVYYSLTPASRLLLKDDPLRAAPFMFLITHPIVTTPFNFLSSWFKNDDPSTPTVFESTHGKDIWGCLAQEAKFKNVFYDAMMTDSQFISSVLIKDCKEVFKGLKCLVDLGGGTGTMAKAIAEAFPEMKCIVFDLPHVVDNNLQQQQGANKNLEFLGGDMFEAIPPADAVLLKWVLHNWGDEESVKILKKCKEAIDGSKDKGGKVIVIDMVIEDQSIDKDSTETQLCCDMLMMSLFSVAKERSIKEWKKLFLEAGFSHYNVNSTLGLRSLIELYP
>Ptrif.0002s0813.1.v1.3.1
MNLIDGEHDTELLEAQAHVWNHIFNFINSMSLKCAVELGIPDIINKHGKPTTLNELVSALTINPSKTRCVYRLMRILIHSGFFAQQTLNSSRNNNDEEQGYVLTNASKLLLKDNPLSVTPFLQAMLDPILLSPWLKLSTWFQNDDPTPFDTAHGKSFWEYAGDEPKINNFFNEAMASDARLATRVVIHKCKDVFEGLNSLVDVGGGTGTVAKAIAKAFPNLECTDFDLPHVVNGLESDLANLKYVGGDMFEAIPPADAVLFKWILHDWNDEECVKILKKCKEAITSDGKKGKVIIIDMMRENKKRDSESIETQLFFDMLMMVLVTGTERDEKEWAKLFADAGFSDYKITPILGLRSLIEVYP
>Ptrif.0002s2820.1.v1.3.1
MSLKCAVELGIADVIHSHGRPITLSELASALNIQPTKTRSLFHFMRLFVHVGLFSKTKVVNHNEQEEAYGLTPTSTLLIKDNSYCLSPLVTGLLDQDFVSSFQYLSSWFKANDLTLWETVRGLKFWDYLNQNPVLSQQFNQVIESDSVMATLLVKDCRPIFQGLRSMVDVGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVEGDMFQFIPPADAFFFKTVFHFFDVEDCLKLLKKCREAIASNGERGKHYNRIGFSWHGKYAVNGI
>Ptrif.0002s2822.1.v1.3.1
MGDIENDRVTVRELFEGQAHLYKGIVKNLSSMSLKCAVELGIADAIHSHGGPITLSELASALNIQPTKTRSLFHFMRLLVHMGLFSKTKVDNHNEQEEAYGLTPTSTLLIKDKPYCLSPLVTGLLDQDYVSSFQHLSRWFKGNDLTLWESFRGLKFWDYLNQNTPLTKRFNQAMTSDSEMATLIVKDCKPIFQGLRSLVDIGGGTGAFARIISEAFPGIKCTVLDLPHAVTDMPQTDNLKYVEGDMFQFIPPADAFFFKTVFHLFDDEDCLKLLKKCREAIASNGERGKVLIVDIVINEKEDDSELTEAKLLFDTLMNFNVGGRERTEQEWESLFVNAGFTHYKVAPIFGIKSLIEVYP
>Ptrif.0002s3316.1.v1.3.1
MINGENAASSSELLIAQAQVWNCTFNYVNSMSLKCAVELGIADVIHSHGQPTTLSQIASALDIKPNKVECFQSVMRTLVHSGFFAQQKDDEYFLTPASRLLLKDTPLKAAPFVDLVADPLYTTAFHCLGTWLQNDDPTLFETAHGKKVWDRLIAGIVIKDCKEVFEGLKSLIDVAGGTGIMARAIATAFPDIKCTVFDLPHVVDNLQGTNDNLDFLGGNMFEAIPQANAVLLKWILHNWNDEESVKLLKKCKEAIPSKDEGGKVIIIDMAIENQSQDKESMETQLCFDILMVSLFRGKERSVDDWKKLFLAAGFSHYKLTPILGVRSLIEAYP
>Ptrif.0002s2409.1.v1.3.1
MDPIQNEGAKEMFEAQAHLFKIIYNYVSSTSLKCAVELDIPEVIHKHGRPITLPQLVSALEINPTKADGLSKLMRLLVHTGFFSKANVQSVQQQQEEAYALTLTSKLFLKDKPYCLSPVVLTLTDPVFVNPCHFLSRWFRDNELSAYETANDGTVFWDYVAKNPDFNNIYNQAMASDSQLANLIVKDCQPIFQGLGSLVDVGGGTGSFARIISEAFPAIKCTVLDLPHVVPKVPDTDNLTFIAGDMFQSIPPADAFFFKAIFHAFVDEDSLKILKRCREAIASRGDRGKVIIIDIVINEKKEDAQLTEAKLLYDMLMMVAVRGSERTEKEWEKLFLDAGFSHFKITPVYGIKSLIEVYP
>Ptrif.0005s3043.1.v1.3.1
MIADFELITSIVVEDCKEVFKGLKSLVDVGGGTRTMARAIATGFLDIKCIVLICLLLWGTNKLEFF
>Ptrif.0005s0416.1.v1.3.1
MSLPMCLCDVLACVERDYAHGHITWLQNDDQTLFGTADGKNFWDYIADEVNFKSIFYEAMITDFELIASVLIEDCTEVTGTMARAIATAFPDTKCTVFDLPLVVDNLVGINNLDFFGGNMFEAIPTPNAVLLKIFNNYSNLKKLTNQLTWWILHDWSDEESLKILKKCKESIPSTERKSKETMETQLCFDMLVATFLKGKERSVYYWKKLFLAAGFSHYKITPNLGLWSLIEAYP
>Ptrif.0006s0175.1.v1.3.1
MDLIDGDHDTELLEAQTHVWNHIFNFINSMTLKCAIQLGIPDIISKHGKPMTLNELVSALTINPSKSRCVYRLMRILIHTSFFAQQKLISSRNNNDEEQGYVLTNASKLLLKDNPLSVTPFLQTMLDPILLSPWQQLSTWFQNDDPTPFDTAHGKTLWEYAGDEPKLNNFFNEAMASDARLATSVMIHKCKDVFEGLNSLVDVGGGIGTAAKVIAKAFPKLECTCFDLPHVVNGLDSDLANLKYVGGDMFEAISPADAVLLKWILHDWNDEECVKILKKCKEAITSDGKKGKVIIIDMIRENKKGDDKSIETQLFFDMLMMVLLTGTERDEKEWAKLFTYAGFSDYKIIPILGLRSLIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University