Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Poncirus trifoliata
Species name
Hardy orange (Citrus trifoliata) is a species of eudicot in the family Rutaceae (rue family).
Species info
Gene sequence info
Poncirus_trifoliata.4CL.fa
Download
>Ptrif.0001s2365.2.v1.3.1
MEARQDHQEFIFRSKLPDIYIPNHLSLHTYCFENISQFADRPCIINGPTGDVYTHADVELTSRKVAAGLSKLGVQKGDVIMLLLQNCPQFVFAFLGASYIGATSTTANPFYTPAEIAKQAIASKAKIIITQAVYNDKVRDLSEHHGARIVTIDPPPENCLHFSELITQADENEIPAVKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVVQQVDGENPNLYLHKDDVILCVLPLFHIYSLNSVLLCALRVGAAILIMQKFDIVKLMELVQKYKVTVAPFVPPIVLAVAKSGDVDKYDMSSIRMVMSGAAPMGKELEDAVRAKLPHATLGQGYGMTEAGSVLSMCLAFAKEPFEIKSGACGTVVRNAEMKIVNPDTGISLPRNQAGEICIRGAQIMKGYLNDREATETTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKFKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVVFYKRINRVFFTDSIPKAPSGKILRKDLRMKLAAGDLRN
>Ptrif.0003s2873.1.v1.3.1
MISIATKKPELSLDISSPAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQDRLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGASMIGAVTTTANPFYTSAEILKQFRTSGAKLIITMSQYVDRLPKTDKDFTVITIDAPPENCLHFTVLSEADEDQIPEVAIEPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLINGDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSVAAVVPPLVLALAKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGASLPHNQPGEICIRGPQIMKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKEIIKFKGFQVPPAEIEALLLSHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAIKEYIAKQVVFYKRLHKIYFVHAIPKSPSGKILRKDLRAKLASSMPLN
>Ptrif.0004s1939.1.v1.3.1
MAMPSSFSVPVPLAAAHDEEHIFRSRHPAVPVPENVTLPDFVLQDADLYADKVAFVEAVSGKAYTYAQVARDTRRFAKALRSLGLRKGQVVIVVLPNVAEYGIVALGIMAAGGVFSGANPTAHASELKKQVDAADAKLIVSYAPIYEKVKGLGLPIIVLGEERIESAINWSELLEAADKAGNNFSNDDDVKQTDLCALPFSSGTTGISKGVMLTHRNLVANLCSTLFSVVPEMIGQVTTLGLIPFFHIYGITGICCATLRNKGKVVMMNRFELRAFLDALITHEVTFAPIVPPIILSLVKDPIVDEFDLSKLQLRAVMTAAAPLAPDLLSSFEKKLPGVQVQEAYGLTEHSCITLTYGDPANPSAGSKKNSVGFILPNLEVKFINPETGKSLPSNTPGELCVRSQCVMQGYYNNEEETARTIDKNGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVSLPDEEAGEIPAACVVMNPNAKESEDDIMDFVASNVATYKKVRVLHFVDGIPKSHSGKIMRRILKDKMLEKMRK
>Ptrif.0005s0930.6.v1.3.1
MMEKFLRTSSSKRINTSQWYSPETGIYHSKHPSVNLPANDSLLDVVSFIFSHKHNGRTALVDSSSGCSISYPQLFPLVQSVASGLRHHFGISQGDAVLLLLPNSIYFPVIFLALLYLGAIVIPMNPLFSVSEIKKQVTLCKTKISLAFTIYENVDKLRSRGTTPVIAVPENLVNDFKEGRFSDFHKLITGKFDLVDRPVIRQQDTAAILYSSGTTGASKVLPMFHIYGLSLFALGLLSLGASVVVMRRFEASEMLKVIDKYGVTHFPVVPPILMALTKAAKGLCGNSLKSLKQVSSGAAPANSKIIEDFVGAFPHVDFIQGYGMTESTAVGTRGFNTKKFSKYFSVGLLAPNMQAKVVDWVNGCFLPPGSTGELWLRGPGTMKEYINNADATMSTIDKEGWLQTGDIAYFDEDGYLYIVDRLKEVIKYNGYQISPADLETVLICHPEILDVAVTGAMDKVLGEIPVAFVVRRDGSTLTEAAVIDYIAQRVAPYKKVRRVVFTKSIPKSAAGKVLRRELRKFLTSKL
>Ptrif.0007s1771.1.v1.3.1
MFIKKASVRNHFHHTNFLVSSMETQTQTQTQTKQQEEIIFRSKLPDIYIPKHLPLHSYCFENISKVASRPCLINGSTGDVYTYGEVELTARKVASGLNKLGIGQRDVIMLLLPNVPEFVLSFLGASYRGAIATAANPFFTPAETCKQAKASGAKLIITKASYVDKVKDLEEVKIMCIDSPPDGCLHFSELTQADEKDLPHVEILPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFTKEDAILCVLPLFHIYALNSIMLCGLRAGAAILIMQKFEINSLLQLIERYKVTVAPVVPPIVLAIAKSPETEKYDLSSIRILKSGAAPLGKELEDAVRIKLPNAILGQGYGMTEAGPVLAMCLSFAKEPFEIKAGACGTVVRNAEMKIVDPETGASLPTNKPGEICIRGDQIMTGYLNDPEATKSTIDKEGWLHTGDIGYIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLSHSSITDAAVVPMKDDAAGEVPVAFVVKSNDSQITEDEIKQFISKQVVFYKRINRVFFIDAIPKAPSGKILRKELRAKLASDYQN
>Ptrif.0008s1926.2.v1.3.1
MEKSGYGRDGIYRSLRPPLVLPSEPNLSMVHFLFRNSASYPSKPALIDADSGESLTFSQLKSTVIKASHGFRHLGITKNDVVMIFAPNSIQFPVCFLGVIATGAIATTANPVYSVSELSKQVIDSNPKLIVTVPELWDKVKDFNLPAVLLGSKSKLASCGLSSSSKIVHFHDLIELSGNISNFPDVSIKQTDTAALLYSSGTTGVSKGVILTHKNFIASSLMVTMDQETAGELDYVFLCVLPLFHVFGLAVITCGQLQKGNCIILMAKFDLEMFLRAIEKFRVTHIWVVPPLILALAKHGLVKKFDLSSLKLVGSGAAPLGKELMEECAKNVPSATVIQGYGLTETSGIATMENSFAGSRNIGSAGALAPGVEALIVSVDTQKPLPPNQLGEIWLRGPNMMQGYYNNEQATKLTIDKKGWVHTGDLGYFDGDGQLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAEAGEVPIAYVVRSPNSSLTEEDVQKFIANQVAPFKRLRRVTFINSVPKSASGKILRRELIAKVRAKM
>Ptrif.0008s1923.3.v1.3.1
MEKSGYGRDGIYKWAALVLPQDPNLSMVHFLFRNSASYGSKSALIDADSGESLTFSQFKCIVIKVSHGFRHLGVTKHDVVLIFAPNSIQFPICLLGVIAIGGIATTANPVYTVSELSKQVKDSSPKLVVTVPELWERVKDFNIPAVLLGSNHIVSPRGLRSSSNIVYFDELIELSGNASNFPDVSVKQTDTAALLYSSGTTGVGKGVILTHKNFIASSLMTAMDQELAGALDYENFIASSLMTAMDQELAGALDYVTLCVLPMFHVFGLSFVVYAQLQKGNCVVSMWKFDLETALRAIEKYSVTHWWVVPPIILALAKNVSLVKKFDLSSLKGIGSGAAPLRKELVEECSKNLPTVTVFQGYGMTETCGVVTVENPLLGVQNSGSAGTLVAGVEAQIVCVDTLKPLPPNQVGELWVRGPILMPGYFNNEQATKLIIDKEGWLHTGDLGSFDEHGQLFIVDRIKELIKCNGFQVAPAELEGVLVSHPEILDAVVIPFPDPEAGEVPIAYVVRSPSSSLTKEDVQKFVVKQVARYKRLRNVTFTNNIPKTTSGKILRRELIAKLRSKI
>Ptrif.0008s1922.1.v1.3.1
MEKSGYGRDGIYRSLRPPLVLPSDPGFSMVHFLFRNSASYSSKLALIDADSDESLSFSQFKSIVIKVSHSFRHLGITKKDVVLIFAPNSIQFPICFLGVIAIGAIASTANPVYTVSELSKQVKDSNPKLVITVPELWDKVKGLNLPAVLLGSKDKVSSSGLISRSSKIVSFHDLIELSGSVTDIPDVSVKQTDAAALLYSSGTTGVSKGVILTHKNFIAASLMISADQELAGELDHVVLCVLPMFHVFGLSVILYGQLQKGNCVVSMGKFDIEMALRAIEKYRVTVWWVVPPIILALAKNSLVRKFDISSLKLVGSSAAPLGKELMEDCQKNIPGATIFQGYGMTEACAPISLENPLVGVRLSGSAGTLLAGVEAQIVSVDTLKPLPPNQLGEIWVRGPNVTPGYFKNEQATKQTIDERGWLHTGDLGYFDGDGQLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDPESGEVPIAYVVRSPNSSLTAEDVQNFVAEQVSPYKRLRKVTFINSVPKSASGKILRRELIAKVRSKI
MEARQDHQEFIFRSKLPDIYIPNHLSLHTYCFENISQFADRPCIINGPTGDVYTHADVELTSRKVAAGLSKLGVQKGDVIMLLLQNCPQFVFAFLGASYIGATSTTANPFYTPAEIAKQAIASKAKIIITQAVYNDKVRDLSEHHGARIVTIDPPPENCLHFSELITQADENEIPAVKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVVQQVDGENPNLYLHKDDVILCVLPLFHIYSLNSVLLCALRVGAAILIMQKFDIVKLMELVQKYKVTVAPFVPPIVLAVAKSGDVDKYDMSSIRMVMSGAAPMGKELEDAVRAKLPHATLGQGYGMTEAGSVLSMCLAFAKEPFEIKSGACGTVVRNAEMKIVNPDTGISLPRNQAGEICIRGAQIMKGYLNDREATETTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKFKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVVFYKRINRVFFTDSIPKAPSGKILRKDLRMKLAAGDLRN
>Ptrif.0003s2873.1.v1.3.1
MISIATKKPELSLDISSPAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQDRLSDDPCLIVGSTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGASMIGAVTTTANPFYTSAEILKQFRTSGAKLIITMSQYVDRLPKTDKDFTVITIDAPPENCLHFTVLSEADEDQIPEVAIEPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLINGDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSVAAVVPPLVLALAKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGASLPHNQPGEICIRGPQIMKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKEIIKFKGFQVPPAEIEALLLSHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAIKEYIAKQVVFYKRLHKIYFVHAIPKSPSGKILRKDLRAKLASSMPLN
>Ptrif.0004s1939.1.v1.3.1
MAMPSSFSVPVPLAAAHDEEHIFRSRHPAVPVPENVTLPDFVLQDADLYADKVAFVEAVSGKAYTYAQVARDTRRFAKALRSLGLRKGQVVIVVLPNVAEYGIVALGIMAAGGVFSGANPTAHASELKKQVDAADAKLIVSYAPIYEKVKGLGLPIIVLGEERIESAINWSELLEAADKAGNNFSNDDDVKQTDLCALPFSSGTTGISKGVMLTHRNLVANLCSTLFSVVPEMIGQVTTLGLIPFFHIYGITGICCATLRNKGKVVMMNRFELRAFLDALITHEVTFAPIVPPIILSLVKDPIVDEFDLSKLQLRAVMTAAAPLAPDLLSSFEKKLPGVQVQEAYGLTEHSCITLTYGDPANPSAGSKKNSVGFILPNLEVKFINPETGKSLPSNTPGELCVRSQCVMQGYYNNEEETARTIDKNGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVSLPDEEAGEIPAACVVMNPNAKESEDDIMDFVASNVATYKKVRVLHFVDGIPKSHSGKIMRRILKDKMLEKMRK
>Ptrif.0005s0930.6.v1.3.1
MMEKFLRTSSSKRINTSQWYSPETGIYHSKHPSVNLPANDSLLDVVSFIFSHKHNGRTALVDSSSGCSISYPQLFPLVQSVASGLRHHFGISQGDAVLLLLPNSIYFPVIFLALLYLGAIVIPMNPLFSVSEIKKQVTLCKTKISLAFTIYENVDKLRSRGTTPVIAVPENLVNDFKEGRFSDFHKLITGKFDLVDRPVIRQQDTAAILYSSGTTGASKVLPMFHIYGLSLFALGLLSLGASVVVMRRFEASEMLKVIDKYGVTHFPVVPPILMALTKAAKGLCGNSLKSLKQVSSGAAPANSKIIEDFVGAFPHVDFIQGYGMTESTAVGTRGFNTKKFSKYFSVGLLAPNMQAKVVDWVNGCFLPPGSTGELWLRGPGTMKEYINNADATMSTIDKEGWLQTGDIAYFDEDGYLYIVDRLKEVIKYNGYQISPADLETVLICHPEILDVAVTGAMDKVLGEIPVAFVVRRDGSTLTEAAVIDYIAQRVAPYKKVRRVVFTKSIPKSAAGKVLRRELRKFLTSKL
>Ptrif.0007s1771.1.v1.3.1
MFIKKASVRNHFHHTNFLVSSMETQTQTQTQTKQQEEIIFRSKLPDIYIPKHLPLHSYCFENISKVASRPCLINGSTGDVYTYGEVELTARKVASGLNKLGIGQRDVIMLLLPNVPEFVLSFLGASYRGAIATAANPFFTPAETCKQAKASGAKLIITKASYVDKVKDLEEVKIMCIDSPPDGCLHFSELTQADEKDLPHVEILPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFTKEDAILCVLPLFHIYALNSIMLCGLRAGAAILIMQKFEINSLLQLIERYKVTVAPVVPPIVLAIAKSPETEKYDLSSIRILKSGAAPLGKELEDAVRIKLPNAILGQGYGMTEAGPVLAMCLSFAKEPFEIKAGACGTVVRNAEMKIVDPETGASLPTNKPGEICIRGDQIMTGYLNDPEATKSTIDKEGWLHTGDIGYIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLSHSSITDAAVVPMKDDAAGEVPVAFVVKSNDSQITEDEIKQFISKQVVFYKRINRVFFIDAIPKAPSGKILRKELRAKLASDYQN
>Ptrif.0008s1926.2.v1.3.1
MEKSGYGRDGIYRSLRPPLVLPSEPNLSMVHFLFRNSASYPSKPALIDADSGESLTFSQLKSTVIKASHGFRHLGITKNDVVMIFAPNSIQFPVCFLGVIATGAIATTANPVYSVSELSKQVIDSNPKLIVTVPELWDKVKDFNLPAVLLGSKSKLASCGLSSSSKIVHFHDLIELSGNISNFPDVSIKQTDTAALLYSSGTTGVSKGVILTHKNFIASSLMVTMDQETAGELDYVFLCVLPLFHVFGLAVITCGQLQKGNCIILMAKFDLEMFLRAIEKFRVTHIWVVPPLILALAKHGLVKKFDLSSLKLVGSGAAPLGKELMEECAKNVPSATVIQGYGLTETSGIATMENSFAGSRNIGSAGALAPGVEALIVSVDTQKPLPPNQLGEIWLRGPNMMQGYYNNEQATKLTIDKKGWVHTGDLGYFDGDGQLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAEAGEVPIAYVVRSPNSSLTEEDVQKFIANQVAPFKRLRRVTFINSVPKSASGKILRRELIAKVRAKM
>Ptrif.0008s1923.3.v1.3.1
MEKSGYGRDGIYKWAALVLPQDPNLSMVHFLFRNSASYGSKSALIDADSGESLTFSQFKCIVIKVSHGFRHLGVTKHDVVLIFAPNSIQFPICLLGVIAIGGIATTANPVYTVSELSKQVKDSSPKLVVTVPELWERVKDFNIPAVLLGSNHIVSPRGLRSSSNIVYFDELIELSGNASNFPDVSVKQTDTAALLYSSGTTGVGKGVILTHKNFIASSLMTAMDQELAGALDYENFIASSLMTAMDQELAGALDYVTLCVLPMFHVFGLSFVVYAQLQKGNCVVSMWKFDLETALRAIEKYSVTHWWVVPPIILALAKNVSLVKKFDLSSLKGIGSGAAPLRKELVEECSKNLPTVTVFQGYGMTETCGVVTVENPLLGVQNSGSAGTLVAGVEAQIVCVDTLKPLPPNQVGELWVRGPILMPGYFNNEQATKLIIDKEGWLHTGDLGSFDEHGQLFIVDRIKELIKCNGFQVAPAELEGVLVSHPEILDAVVIPFPDPEAGEVPIAYVVRSPSSSLTKEDVQKFVVKQVARYKRLRNVTFTNNIPKTTSGKILRRELIAKLRSKI
>Ptrif.0008s1922.1.v1.3.1
MEKSGYGRDGIYRSLRPPLVLPSDPGFSMVHFLFRNSASYSSKLALIDADSDESLSFSQFKSIVIKVSHSFRHLGITKKDVVLIFAPNSIQFPICFLGVIAIGAIASTANPVYTVSELSKQVKDSNPKLVITVPELWDKVKGLNLPAVLLGSKDKVSSSGLISRSSKIVSFHDLIELSGSVTDIPDVSVKQTDAAALLYSSGTTGVSKGVILTHKNFIAASLMISADQELAGELDHVVLCVLPMFHVFGLSVILYGQLQKGNCVVSMGKFDIEMALRAIEKYRVTVWWVVPPIILALAKNSLVRKFDISSLKLVGSSAAPLGKELMEDCQKNIPGATIFQGYGMTEACAPISLENPLVGVRLSGSAGTLLAGVEAQIVSVDTLKPLPPNQLGEIWVRGPNVTPGYFKNEQATKQTIDERGWLHTGDLGYFDGDGQLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDPESGEVPIAYVVRSPNSSLTAEDVQNFVAEQVSPYKRLRKVTFINSVPKSASGKILRRELIAKVRSKI
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University