Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Apostasia shenzhenica
Species name
Apostasia shenzhenica is a species of monocot in the family Orchidaceae (orchid family).
Species info
Gene sequence info
Apostasia_shenzhenica.4CL.fa
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>rna-gnl|WGS:PEFY|mrna.Ash012429
MGDAAMEEEQRQLDQKEHIFRSNYSSMPVPADVTLPEFVLSGAEQYADKVALVEAATGKSLTYGQVARETRRFAGALRSLGLRKGHVVVVALPNLALYPVVALGVMAAGGVFSGVNPLATPAEMRKQVEDSEAKLLVANDSTYEKVKDLGLPTITLGEERLPGAILWEDLLEASDRAGVCQSDLCALPYSSGTTGTSKGVMLTHRNLIANLCCSLFSVPAEMVGQATVLGLMPFFHIYGITGICCAALRNRGTIVVLQRFELRSFLCALIDYEVTFAPIVPPIMLAMVNSSVAGEFDLSRLKLRSVMSAAAPLAPELRAAFEEKFPGVEIQEAYGLTEHSCITLTHGDQRKGQRVAKKSSVGFILPNIEVKFIDPDSTQSLPKNSPGELCVRSQCVMQGYYKNKEETQRTVDGGGWLHTGDVGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLAHPSVDDAAVFGLPEEEAGEIPTAAIVKKADKAESEEEIMMYVAGKVASYKRVRAVHFVDTIPRSPSGKIMRRVLKKRFAETDK
>rna-gnl|WGS:PEFY|mrna.Ash018560
MGSYSPETAVSGDLIFRSRLPVIDIPDHLPLHDYCFANLPAVAHRPCLINGSTGDILSYSDVHLLSRRAAAGLRRLGVRSGDVIMLLLPNSPEFAISFLGASLLGAVSTTANPFYTPAEVHKQARAAGACLVVTEACHLHKVKGLGIEIVCVNRDITPDGCWCFADVVEADEKELPEAEIQPDDVVALPYSSGTTGLPKGVLLTHRGLVTSVAQQVDGENPNLYYDKDDVLLCVLPLFHIYSLNSVLLCGLRAGAAIVIMRKFELGRMMELVERYRVTVAPFVPPIVVEVTKDGTAGVSGYDLSSIRTVMCGAAPMGRGLQDAFRAVLPNAKLGQGYGMTEAGPVLSMSLAFAKEPCEVKSGSCGTVVRNAELKVIDIETGHSLSRNKAGEICIRGNQIMKGYLNDPEATRQIIDKEGWLHTGDIGYVDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPKIIDVAVVSMKDDLCGELPVAFIVRSNGSEVTEDEIKQYVSKQVIFYKRIHRVFTVETIPKSPSGKILRKELRARLETGGCAGRT
>rna-gnl|WGS:PEFY|mrna.Ash012027
MITVASPELHQQDRAPPAVAEPLPAETVVFRSRLPDIPIPNHEPLHAYCFEKLPEFAGNRCLIFGPTGRTHTYAETKVFCRRAAAGLAGLGIKQGDVVLLLLQNCPEFVFTFMAASFLGAAATTANPFCTPAEIYKQFKASGAKLVVTQSLYVDKLRNYTHEFPEFGPELTIITVDEPPEGCSPFSVLYADEEDKVPAVPIDPDDAVALPFSSGTTGLPKGVVLTHRSLITNIAQQVDGENPNLHLRSDDVVLCVLPLFHIFSLSSVLLCSLRAGAAVVIMPKFEIGAMLGLIEKYRVTVTAVVPPLVLALAKNPVVENYDLGSIRIVISGAAPLGKDLEAALRCRLPQAVLGQGYGMTEAGPVISMCPTFAKRPPTFAKSGSCGIVVRNAELKVVDLETGFSCNRSKPGEICIRGAQIMKGYLNDSEATTMTVDLDGWLHTGDIGYVDDDDEVYIVDRVKELIKFKGFQVPPAELEGLLVSHPFIADAAVIPQKDEAAGEVPVAFIVRANDAELTEDDVKEFIAQQVVFYKRLYRVHFVHSIPKSPTGKILRKELRARLANATA
>rna-gnl|WGS:PEFY|mrna.Ash016648
MGSLYTPDAVSGAGEHIFRSKVPDIDIPDHLPLHDYCFAKLPEVAGRPCLIDGASGDVFSYADVHLLSRRAAAGLGRLGVRQGDVIMILLPNTPEFAFAFLGASILGAMSTTANPFYTPAEIHRQARDAGTRLIVTEACYVHKVRDLAGVDVVCIGETPVPEGCRSFAELLSADEKELPEVEIKAEDVVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGENPNLYFDADDVLICFLPLFHIYSLNSLLLCSLRAGAAMVVMRKFDLERMMELVERYRVTVAPFVPPVVVEMAKNAAVERYDLSSVRIIMSGAAPMGKELEDTLRAKLPNAKFAQGYGMTEAGVLSMSLLFAKQPFESKSGACGTVMRNAELKIIEPESGRSLPPSQPGEICIRGNQIMKGYLNNHEATEQTIDKEGWLHTGDIGYVDEDDEIFIVDRLKELIKYKGFQVAPAELEALLITHPAIASAAVVSMKDDLCGELPVAFVVLSNGSEVTEEEIKKYISKQVVFYKRIHRVFFVEAIPKSPAGKILRKVLRARLAAGEPNGRTQ
>rna-gnl|WGS:PEFY|mrna.Ash018517
MTEAGPVLSFCMSFAKEPMPSKPGSCGMVVRNAELKVVDVSTGASLRRNQRGEICIRGAQIMKGYLNNETATKATVDGDGWLHTGDIGYVDDDDDIFIVDRLKEIIKYKGFQVAPAEIEALLLAHDHVVDAAVVAMKDDAAGEVPVAFIVRVKGSELSEDEIKRYIAKQVVFYKRISRAFFMQAIPKSPSGKILRKVLTQKLEEGLHNLC
>rna-gnl|WGS:PEFY|mrna.Ash015800
MAFSGAIYGADGIYRSPRPLVSFPSDPSLSMITFLFRNASSYPDRIAVVDADSNDSLTFLQLQSSVHSAAAGFRRLGIGKGSIVLIFAPNSIHFLVAFLAVVAVGAIATTVNPLYTVAELSKQGKDSGAQLVVSVPQLLDKVKSLGLPIVLLGSKTTSATDGSETPIYYFSNFLALPKSEYSPPPISQSDIAALLYSSGTTGASKGVILNHRNFICASLMTSGDQDLRGEPPNTFLLFLPLFHIFGLSVIAYGQLQRGNSLVVMERFDMETMLRSIQKHKVSHVWVVPPVMIALAKQGKVTKYNLSSLRFVGSGAAPLGKDVMEEVAKNIPTAQILQGYGMTETTGIISLEYPTGEGRHYGSTGFLAVGVDGKVINVDTQMPLPPNQLGELCFRGPNIMLGYLNNPEATRSTLDEEGWLHTGDLGYFDEKGQLFVADRIKELIKYKGFQVAPAELEGLLLAHPEIIDAVVIPFPDAEAGEVPIAYVVRSPNSSLTELDVKQFIADQVAPFKRLRRVSFVNSVPKSASGKILRRELIQKVKSNL
>rna-gnl|WGS:PEFY|mrna.Ash016950
MEPSKHGHDAAGIYRSPRPAISFPSDPSLTMIPFLFRNTTAYSSRTALIDADSGNSLTFSELRSAVNSVAAGLISLGVTKGSVVLIAAPNSLHFPVAYFAIVAAGAVVTTTNPLSTSRELSNHVNDSGAVLIFTLPQLWDKIQHLRLPTVFFGPKKRATAVAAGSKLTQTVHYLADLLAVPESDFRPPEIKQSDTAALPYSSGTTGKSKGVVLTHRNIIYASLMTVSDQEATAGDSHTTVLCFLPLFHFFGLGAAAFAQLQRGNCVAVMARYEMEAVLRAVERYRAGYLFVVPPVMAALARLGRAPKYDLSSLKLIISGAAPVGKEVVEKVAENFPEATVVQAYGMTEASGFISMGYPQKGATHFGSNGFLVSGLEGKVINLETLKSLAPNNIGELCFRGPSIMQGYFNNPDATKLSLDDEGWLHTGDLGYFDENGQLFIVDRIKDLIKYKGFQVAPAELEGLLISHPQILDAAVIPFPDAEAGEVPAAYVVRTLTSSLTEDEVKKFIADQVAPFKRVRRVSFIDSVPKSASGKILRRELIRKVRPAL
MGDAAMEEEQRQLDQKEHIFRSNYSSMPVPADVTLPEFVLSGAEQYADKVALVEAATGKSLTYGQVARETRRFAGALRSLGLRKGHVVVVALPNLALYPVVALGVMAAGGVFSGVNPLATPAEMRKQVEDSEAKLLVANDSTYEKVKDLGLPTITLGEERLPGAILWEDLLEASDRAGVCQSDLCALPYSSGTTGTSKGVMLTHRNLIANLCCSLFSVPAEMVGQATVLGLMPFFHIYGITGICCAALRNRGTIVVLQRFELRSFLCALIDYEVTFAPIVPPIMLAMVNSSVAGEFDLSRLKLRSVMSAAAPLAPELRAAFEEKFPGVEIQEAYGLTEHSCITLTHGDQRKGQRVAKKSSVGFILPNIEVKFIDPDSTQSLPKNSPGELCVRSQCVMQGYYKNKEETQRTVDGGGWLHTGDVGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLAHPSVDDAAVFGLPEEEAGEIPTAAIVKKADKAESEEEIMMYVAGKVASYKRVRAVHFVDTIPRSPSGKIMRRVLKKRFAETDK
>rna-gnl|WGS:PEFY|mrna.Ash018560
MGSYSPETAVSGDLIFRSRLPVIDIPDHLPLHDYCFANLPAVAHRPCLINGSTGDILSYSDVHLLSRRAAAGLRRLGVRSGDVIMLLLPNSPEFAISFLGASLLGAVSTTANPFYTPAEVHKQARAAGACLVVTEACHLHKVKGLGIEIVCVNRDITPDGCWCFADVVEADEKELPEAEIQPDDVVALPYSSGTTGLPKGVLLTHRGLVTSVAQQVDGENPNLYYDKDDVLLCVLPLFHIYSLNSVLLCGLRAGAAIVIMRKFELGRMMELVERYRVTVAPFVPPIVVEVTKDGTAGVSGYDLSSIRTVMCGAAPMGRGLQDAFRAVLPNAKLGQGYGMTEAGPVLSMSLAFAKEPCEVKSGSCGTVVRNAELKVIDIETGHSLSRNKAGEICIRGNQIMKGYLNDPEATRQIIDKEGWLHTGDIGYVDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLIAHPKIIDVAVVSMKDDLCGELPVAFIVRSNGSEVTEDEIKQYVSKQVIFYKRIHRVFTVETIPKSPSGKILRKELRARLETGGCAGRT
>rna-gnl|WGS:PEFY|mrna.Ash012027
MITVASPELHQQDRAPPAVAEPLPAETVVFRSRLPDIPIPNHEPLHAYCFEKLPEFAGNRCLIFGPTGRTHTYAETKVFCRRAAAGLAGLGIKQGDVVLLLLQNCPEFVFTFMAASFLGAAATTANPFCTPAEIYKQFKASGAKLVVTQSLYVDKLRNYTHEFPEFGPELTIITVDEPPEGCSPFSVLYADEEDKVPAVPIDPDDAVALPFSSGTTGLPKGVVLTHRSLITNIAQQVDGENPNLHLRSDDVVLCVLPLFHIFSLSSVLLCSLRAGAAVVIMPKFEIGAMLGLIEKYRVTVTAVVPPLVLALAKNPVVENYDLGSIRIVISGAAPLGKDLEAALRCRLPQAVLGQGYGMTEAGPVISMCPTFAKRPPTFAKSGSCGIVVRNAELKVVDLETGFSCNRSKPGEICIRGAQIMKGYLNDSEATTMTVDLDGWLHTGDIGYVDDDDEVYIVDRVKELIKFKGFQVPPAELEGLLVSHPFIADAAVIPQKDEAAGEVPVAFIVRANDAELTEDDVKEFIAQQVVFYKRLYRVHFVHSIPKSPTGKILRKELRARLANATA
>rna-gnl|WGS:PEFY|mrna.Ash016648
MGSLYTPDAVSGAGEHIFRSKVPDIDIPDHLPLHDYCFAKLPEVAGRPCLIDGASGDVFSYADVHLLSRRAAAGLGRLGVRQGDVIMILLPNTPEFAFAFLGASILGAMSTTANPFYTPAEIHRQARDAGTRLIVTEACYVHKVRDLAGVDVVCIGETPVPEGCRSFAELLSADEKELPEVEIKAEDVVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGENPNLYFDADDVLICFLPLFHIYSLNSLLLCSLRAGAAMVVMRKFDLERMMELVERYRVTVAPFVPPVVVEMAKNAAVERYDLSSVRIIMSGAAPMGKELEDTLRAKLPNAKFAQGYGMTEAGVLSMSLLFAKQPFESKSGACGTVMRNAELKIIEPESGRSLPPSQPGEICIRGNQIMKGYLNNHEATEQTIDKEGWLHTGDIGYVDEDDEIFIVDRLKELIKYKGFQVAPAELEALLITHPAIASAAVVSMKDDLCGELPVAFVVLSNGSEVTEEEIKKYISKQVVFYKRIHRVFFVEAIPKSPAGKILRKVLRARLAAGEPNGRTQ
>rna-gnl|WGS:PEFY|mrna.Ash018517
MTEAGPVLSFCMSFAKEPMPSKPGSCGMVVRNAELKVVDVSTGASLRRNQRGEICIRGAQIMKGYLNNETATKATVDGDGWLHTGDIGYVDDDDDIFIVDRLKEIIKYKGFQVAPAEIEALLLAHDHVVDAAVVAMKDDAAGEVPVAFIVRVKGSELSEDEIKRYIAKQVVFYKRISRAFFMQAIPKSPSGKILRKVLTQKLEEGLHNLC
>rna-gnl|WGS:PEFY|mrna.Ash015800
MAFSGAIYGADGIYRSPRPLVSFPSDPSLSMITFLFRNASSYPDRIAVVDADSNDSLTFLQLQSSVHSAAAGFRRLGIGKGSIVLIFAPNSIHFLVAFLAVVAVGAIATTVNPLYTVAELSKQGKDSGAQLVVSVPQLLDKVKSLGLPIVLLGSKTTSATDGSETPIYYFSNFLALPKSEYSPPPISQSDIAALLYSSGTTGASKGVILNHRNFICASLMTSGDQDLRGEPPNTFLLFLPLFHIFGLSVIAYGQLQRGNSLVVMERFDMETMLRSIQKHKVSHVWVVPPVMIALAKQGKVTKYNLSSLRFVGSGAAPLGKDVMEEVAKNIPTAQILQGYGMTETTGIISLEYPTGEGRHYGSTGFLAVGVDGKVINVDTQMPLPPNQLGELCFRGPNIMLGYLNNPEATRSTLDEEGWLHTGDLGYFDEKGQLFVADRIKELIKYKGFQVAPAELEGLLLAHPEIIDAVVIPFPDAEAGEVPIAYVVRSPNSSLTELDVKQFIADQVAPFKRLRRVSFVNSVPKSASGKILRRELIQKVKSNL
>rna-gnl|WGS:PEFY|mrna.Ash016950
MEPSKHGHDAAGIYRSPRPAISFPSDPSLTMIPFLFRNTTAYSSRTALIDADSGNSLTFSELRSAVNSVAAGLISLGVTKGSVVLIAAPNSLHFPVAYFAIVAAGAVVTTTNPLSTSRELSNHVNDSGAVLIFTLPQLWDKIQHLRLPTVFFGPKKRATAVAAGSKLTQTVHYLADLLAVPESDFRPPEIKQSDTAALPYSSGTTGKSKGVVLTHRNIIYASLMTVSDQEATAGDSHTTVLCFLPLFHFFGLGAAAFAQLQRGNCVAVMARYEMEAVLRAVERYRAGYLFVVPPVMAALARLGRAPKYDLSSLKLIISGAAPVGKEVVEKVAENFPEATVVQAYGMTEASGFISMGYPQKGATHFGSNGFLVSGLEGKVINLETLKSLAPNNIGELCFRGPSIMQGYFNNPDATKLSLDDEGWLHTGDLGYFDENGQLFIVDRIKDLIKYKGFQVAPAELEGLLISHPQILDAAVIPFPDAEAGEVPAAYVVRTLTSSLTEDEVKKFIADQVAPFKRVRRVSFIDSVPKSASGKILRRELIRKVRPAL
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University