Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Pistacia vera
Species name
Pistachio (Pistacia vera) is a species of eudicot in the family Anacardiaceae (sumac family).
Species info
Gene sequence info
Pistacia_vera.FLS.fa
Download
>rna-XM_031418961.1
MASTSVITFHLEYRANSSTLIPERLSYKTYTLCMQRRGCFPGIPSYFVQDVFQFKLVCAISLKTTSLINQEVSDELLRNMKEQAQEFFALPLQEKKRWAQKPGSLEGYGQAFVTSPQQKLEWNDMLFLKTLPPSIRNQNLWPQKPQKFRETLETYSEDMRKVAISLATFIAMALEIETHEEISRANQEGTYEVRMNLYPPCPESELVIGILPHADISGITLLLECGDMPRLQALKDGQWVTVLPKHGAIVVNLGHITEVCNI
>rna-XM_031419483.1
MEMERVQALAYAGLNELPAQFIRPINERPENSKPISGVTVPVISLSQPRHVIIEEVYKACREWGFFLVTDHGISSALIQQLQEVGQQFFGLPQKEKEAYANDPATGKFEGYGTKMTKNLDEKVEWVDYFFHVMSPKAKTNYDIWPKHPHTYREVTEEYNKEMLRVTNELLELLSEGLGVEGNVLKSHLGGEDIELEMKINMYPPCPQPQLALGVEPHTDMSALTILVPNDVPGLHVWKDGNWVAVNYLPNALFVHVGDQIEVLSNGKYKSVLHRSIVNKERMRMSWAVFAVPPHEALIGPLQELVDDDQNPPKYSTKTFAEYRYRKFNKLPQ
>rna-XM_031431135.1
MELQRVQAIASLSFSKETIPAEFIRPEKEQPAITTYHGPVPEIPTIDLNDPCEENLVRLIAKASREWGIFQVVNHGIPSDVIRKLQAAGKEFFELPQEEKEVYAKPPGAQTVEGYGSKLQKEVEGKKAWVDHLFHKIWPPSSINYQYWPKNPPSYRDANEEYAKYMKEVVDKLFKTLSLGLGVEGHVLKEASGGEDIEYMLKINYYPPCPRPDLALGVVPHTDLSTITILVPNDVPGLQVFKDDRWIDAKYIPNALIVHIGDQIEILSNGVYKAVLHRTTVNKEKTRMSWPVFLEPPGEFVVGPLPQLVGEDNPPKYKAKIFKDYIYCKLNKIPQ
>rna-XM_031394124.1
MAEASILPAEVLLSKRVQEMVLKGEEPPPKYVCRDGDAVHEVAAPFSMIPIIDLSLCASSTPGMGQQEELQKLKSALCSWGCFQAVGHGISSTFLDKFRKVSREYFELPMEEKKKQAKGVEDFEGYGADPVPEEGQFLDWSDRLFLEVYPEDQRNPKLWPEKPESFRGILEEYSIKMKKVTDLTSKAMAKSLNIEENCFLTQFGERAQHQARFNYYSRCQRPDLVLGLKPHSDGSGYTIILMDAEGLQVLKDEQWFTVPKISEALLVLMGDQMEIMTNGIFKSPVHRVVTSKEKERISVAVFYSPEVNKEIGPEDRFINEETPRLFKKVKGYPDIHWGYYQKGQRALHVAKV
>rna-XM_031394146.1
MAEASILPAEVLRSKHVQEMVLKGEEPPPKYVCRDGDAVMIIYISISNAVCHGISSTFLDKFREVSREYFEQPMEEKKKQAKGVEDFEGYGADPVPEEGQFLDWSDRLFLEVYPEDQRNPKLWPENPESFSCILEKYSIKMKKVTELTSKAMAKSLNIEENCFLTQFGERAQYKARFNYYSRCQRPDLVLGLKPHSDGSGYTIIPMDAEGLQVLKDEQWFTVPKISEALLVLMGDQMEIMTNGIFKSPVHRVVTSKEKERISVAIFCSPEVNKDIGPEDGLINEETPRLFKKVKGYADIHWGYYQKGQRALHVAKV
>rna-XM_031394176.1
MEQEEELQKLKSALCSWGCFQEVSHGISSTFLDKFREVSRESFEQPMEEKKKQAKGVEDFEGYGADPVPEEGQFLDLFYRLFLDVYPEDRRNPELWPEKPESFRCFHAPHVSDND
>rna-XM_031396753.1
MEMERVQALAYAGLNELPAQFIRPPNERPENSKPISGVTVPVISLSQPRHVIVEEVYKACSEWGFFLVTDHGISSALIQQLQEVGQQFFGLPQKEKEAYANDPATGKFEGYGTKMTKNLDEKVEWVDYFFHVMSPKAKTNYDMWPKHPHTYREVTEEYNKEMLRVTNELLELLSEGLGVEGNVLKSHLGGEDIELEMKINMYPPCPQPQLALGVEPHTDMSTLTILVPNDVPGLHVFKDGNWVAVNYLSNALFVHVGDQIEAYEKNQDLDQALGQVGFG
>rna-XM_031396765.1
MAIETVQSIVLKEKIPEEFIRSQHEQPGITTVQGTVLEVPVIDLNDHDQEKVLNLIVDASQKWGMFQIVNHGIPFQVLSKLKEVGKEFFELPQEEKEVYAKPPESKGIEGYGTKLQKELQGKKTWNDHLFHKISPPSVINYRFWPKNPPSYREATEEYTKWLHGVAEKLLKSLSLGLGLEEDEVKKALGGENLIYLLKINHYPPCPQPELALGLADHTDMCAITILLPNEVQGLQASRDGHWYDVKYVTNALVVHIGDQMEILSNGKYRAVLHRVAVNKEKTRLSWPVFLEPAVDLEVGPHPKLVDQENPAKYKTKKYGDYAYCKLNKIPQ
>rna-XM_031396751.1
MQRVKALAYAGLNELPAQFIRPPTERPENSKPISGVTVPVISLSQPRHVIVEEVYEACSEWGFFLVTDHGISSALIQQLQEVGQQFFGLPQKEKEAYANDPATGKFEGYGTKMTKNLDENVEWLDYFFHVMSPKAKTNYDIWPKHPHTYREVTEEYNKEMLRVTNELLELLSEGLGVEGNVLKSHLGGEDIELEMKINMYPPCPQPQLALGVEPHTDMSTLTILVPNDVPGLHVWKDGNWVAVNYLPNALFVHVGDQIEVLSNGKYKSVLHRSIVNKERMRMSWAVFAAPPHEALIGPLPELVNDDQKPPKYSTKTFAEYRYRKFNKLSQ
>rna-XM_031399903.1
MAAVSPQSVPEKPLDFRAPPPSPIASSRRSSVTNDDVLTEYLEHSLRVPDLILPDKVFPRQKFIENPPRIDFQTLNSEESCDSVSKIVDSITTIGCFQLVNYGIPSELIKSALAIAAGVFRVSPEKRATVTRSPEKPYGFEEVHGEEESEMSEEFVWCRDENLKLEMEGIWPLGYSKFSEKMETLLGDIEKLGEKILVSLRENCVRKSMHGDEILEEHENVGSVCHIHKHSHNILADFSSLRYDVIRMLIRGVDYSHALCLHICDGASEFHVYSKKGWVSFCPDKDAILITAGDQIQAVSGGQYKHVIGRPVYKEEKGDCISMAFLYAPQTIITNFTPNLEKEKTISLPQQFIVALLLTLLYHFLVFVYKKF
>rna-XM_031400080.1
MDLPVIDLAPYLSAGGKPSGDDDVAKVSSELGQLCREVSRILRETGALLVKDPRCTVEDNDRFIDMMEKYFESPKEFKLLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKLKVMPKESQPSLPRGPDPKWRYMWRVGPRPSNTRFQELNSEPVIPEGFPEWKDTMDSWGYKMISAIEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSRYGQEGTVFAGYHYDLNFLTIHGRSRFPGLNIWLRNGQKVEVKVPVGCLLIQTGKQIEWLTAGDCIAGMHEVIVMSRTINAIKLASEQNRSLWRVSSTLFAHIASDAVLKPLGHFAESPLASKYPPICAGEFVEQELAVINLKGSKGSLDNL
MASTSVITFHLEYRANSSTLIPERLSYKTYTLCMQRRGCFPGIPSYFVQDVFQFKLVCAISLKTTSLINQEVSDELLRNMKEQAQEFFALPLQEKKRWAQKPGSLEGYGQAFVTSPQQKLEWNDMLFLKTLPPSIRNQNLWPQKPQKFRETLETYSEDMRKVAISLATFIAMALEIETHEEISRANQEGTYEVRMNLYPPCPESELVIGILPHADISGITLLLECGDMPRLQALKDGQWVTVLPKHGAIVVNLGHITEVCNI
>rna-XM_031419483.1
MEMERVQALAYAGLNELPAQFIRPINERPENSKPISGVTVPVISLSQPRHVIIEEVYKACREWGFFLVTDHGISSALIQQLQEVGQQFFGLPQKEKEAYANDPATGKFEGYGTKMTKNLDEKVEWVDYFFHVMSPKAKTNYDIWPKHPHTYREVTEEYNKEMLRVTNELLELLSEGLGVEGNVLKSHLGGEDIELEMKINMYPPCPQPQLALGVEPHTDMSALTILVPNDVPGLHVWKDGNWVAVNYLPNALFVHVGDQIEVLSNGKYKSVLHRSIVNKERMRMSWAVFAVPPHEALIGPLQELVDDDQNPPKYSTKTFAEYRYRKFNKLPQ
>rna-XM_031431135.1
MELQRVQAIASLSFSKETIPAEFIRPEKEQPAITTYHGPVPEIPTIDLNDPCEENLVRLIAKASREWGIFQVVNHGIPSDVIRKLQAAGKEFFELPQEEKEVYAKPPGAQTVEGYGSKLQKEVEGKKAWVDHLFHKIWPPSSINYQYWPKNPPSYRDANEEYAKYMKEVVDKLFKTLSLGLGVEGHVLKEASGGEDIEYMLKINYYPPCPRPDLALGVVPHTDLSTITILVPNDVPGLQVFKDDRWIDAKYIPNALIVHIGDQIEILSNGVYKAVLHRTTVNKEKTRMSWPVFLEPPGEFVVGPLPQLVGEDNPPKYKAKIFKDYIYCKLNKIPQ
>rna-XM_031394124.1
MAEASILPAEVLLSKRVQEMVLKGEEPPPKYVCRDGDAVHEVAAPFSMIPIIDLSLCASSTPGMGQQEELQKLKSALCSWGCFQAVGHGISSTFLDKFRKVSREYFELPMEEKKKQAKGVEDFEGYGADPVPEEGQFLDWSDRLFLEVYPEDQRNPKLWPEKPESFRGILEEYSIKMKKVTDLTSKAMAKSLNIEENCFLTQFGERAQHQARFNYYSRCQRPDLVLGLKPHSDGSGYTIILMDAEGLQVLKDEQWFTVPKISEALLVLMGDQMEIMTNGIFKSPVHRVVTSKEKERISVAVFYSPEVNKEIGPEDRFINEETPRLFKKVKGYPDIHWGYYQKGQRALHVAKV
>rna-XM_031394146.1
MAEASILPAEVLRSKHVQEMVLKGEEPPPKYVCRDGDAVMIIYISISNAVCHGISSTFLDKFREVSREYFEQPMEEKKKQAKGVEDFEGYGADPVPEEGQFLDWSDRLFLEVYPEDQRNPKLWPENPESFSCILEKYSIKMKKVTELTSKAMAKSLNIEENCFLTQFGERAQYKARFNYYSRCQRPDLVLGLKPHSDGSGYTIIPMDAEGLQVLKDEQWFTVPKISEALLVLMGDQMEIMTNGIFKSPVHRVVTSKEKERISVAIFCSPEVNKDIGPEDGLINEETPRLFKKVKGYADIHWGYYQKGQRALHVAKV
>rna-XM_031394176.1
MEQEEELQKLKSALCSWGCFQEVSHGISSTFLDKFREVSRESFEQPMEEKKKQAKGVEDFEGYGADPVPEEGQFLDLFYRLFLDVYPEDRRNPELWPEKPESFRCFHAPHVSDND
>rna-XM_031396753.1
MEMERVQALAYAGLNELPAQFIRPPNERPENSKPISGVTVPVISLSQPRHVIVEEVYKACSEWGFFLVTDHGISSALIQQLQEVGQQFFGLPQKEKEAYANDPATGKFEGYGTKMTKNLDEKVEWVDYFFHVMSPKAKTNYDMWPKHPHTYREVTEEYNKEMLRVTNELLELLSEGLGVEGNVLKSHLGGEDIELEMKINMYPPCPQPQLALGVEPHTDMSTLTILVPNDVPGLHVFKDGNWVAVNYLSNALFVHVGDQIEAYEKNQDLDQALGQVGFG
>rna-XM_031396765.1
MAIETVQSIVLKEKIPEEFIRSQHEQPGITTVQGTVLEVPVIDLNDHDQEKVLNLIVDASQKWGMFQIVNHGIPFQVLSKLKEVGKEFFELPQEEKEVYAKPPESKGIEGYGTKLQKELQGKKTWNDHLFHKISPPSVINYRFWPKNPPSYREATEEYTKWLHGVAEKLLKSLSLGLGLEEDEVKKALGGENLIYLLKINHYPPCPQPELALGLADHTDMCAITILLPNEVQGLQASRDGHWYDVKYVTNALVVHIGDQMEILSNGKYRAVLHRVAVNKEKTRLSWPVFLEPAVDLEVGPHPKLVDQENPAKYKTKKYGDYAYCKLNKIPQ
>rna-XM_031396751.1
MQRVKALAYAGLNELPAQFIRPPTERPENSKPISGVTVPVISLSQPRHVIVEEVYEACSEWGFFLVTDHGISSALIQQLQEVGQQFFGLPQKEKEAYANDPATGKFEGYGTKMTKNLDENVEWLDYFFHVMSPKAKTNYDIWPKHPHTYREVTEEYNKEMLRVTNELLELLSEGLGVEGNVLKSHLGGEDIELEMKINMYPPCPQPQLALGVEPHTDMSTLTILVPNDVPGLHVWKDGNWVAVNYLPNALFVHVGDQIEVLSNGKYKSVLHRSIVNKERMRMSWAVFAAPPHEALIGPLPELVNDDQKPPKYSTKTFAEYRYRKFNKLSQ
>rna-XM_031399903.1
MAAVSPQSVPEKPLDFRAPPPSPIASSRRSSVTNDDVLTEYLEHSLRVPDLILPDKVFPRQKFIENPPRIDFQTLNSEESCDSVSKIVDSITTIGCFQLVNYGIPSELIKSALAIAAGVFRVSPEKRATVTRSPEKPYGFEEVHGEEESEMSEEFVWCRDENLKLEMEGIWPLGYSKFSEKMETLLGDIEKLGEKILVSLRENCVRKSMHGDEILEEHENVGSVCHIHKHSHNILADFSSLRYDVIRMLIRGVDYSHALCLHICDGASEFHVYSKKGWVSFCPDKDAILITAGDQIQAVSGGQYKHVIGRPVYKEEKGDCISMAFLYAPQTIITNFTPNLEKEKTISLPQQFIVALLLTLLYHFLVFVYKKF
>rna-XM_031400080.1
MDLPVIDLAPYLSAGGKPSGDDDVAKVSSELGQLCREVSRILRETGALLVKDPRCTVEDNDRFIDMMEKYFESPKEFKLLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKLKVMPKESQPSLPRGPDPKWRYMWRVGPRPSNTRFQELNSEPVIPEGFPEWKDTMDSWGYKMISAIEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSRYGQEGTVFAGYHYDLNFLTIHGRSRFPGLNIWLRNGQKVEVKVPVGCLLIQTGKQIEWLTAGDCIAGMHEVIVMSRTINAIKLASEQNRSLWRVSSTLFAHIASDAVLKPLGHFAESPLASKYPPICAGEFVEQELAVINLKGSKGSLDNL
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University