Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Piper nigrum
Species name
Piper nigrum is a species of flowering plant in the family Piperaceae (pepper family).
Species info
Gene sequence info
Piper_nigrum.ROMT.fa
Download
>evm.model.Pn8.922
MGRCWCGTGSAAKVIAEAFTCIKCTVLDPAPCGRRSAGDPRGGNILHDWSDEECVQILQRCKEAIPSREEGGKVILVELVAGAKPNDSTYMETIFYLDLLMAAHADGKEREEHELKKIFQDAGFSHYKITPALGLRSVIELFP
>evm.model.Pn37.3
MQHFLCAIVLLLIVQPSIFAYARNSHRDRKMLVDPLGSISNKNKSLKSSHPIPVIDDVLEPEDYPPPGPNRPLPPARNGACEEQRVMNTEETWHAESLLWNNFYSFVKSMALKCAIRLRIPEIINNHGPDFNKA
>evm.model.Pn25.230
MGHAKCNERVMNTEETWQAESLLWNNFYSFVKSMALKCAIRLRIPEIIHNHGRPAALHEVMAAVPIPAAKADPLRRLMRLLTDSGIFAIDQNGAYALTPASRLLLPGPGGTSLALSSLLSLEPDVVGVWDHLDEWYKVEDGAKTPFQVTYRMNTWDYCRSQPRLQGLLNDAMAADSEHLMDALVNNCGDVFRGLRSVVDAAGGVGMAAKTIARAFPEIEVTVLDQPHVVAGLPEKEDDSGVKFVGGDIFKSIPPANAVFLKWVLHDWTDEECIEILKKCKEAIPSKEAGGKVIIVDMVVDPSCSNGVARETQFAYDVFMLVVSMGKEREKEEWKKIFEDAGFSDCKFTPALGYRCVIEVYP
>evm.model.Pn25.237
MIRVLHDWTDEECVEILKKCKEAIPSKEARGKVIIVDMVVDPSCSNGVARETQFAYDVFMLVVSMGKEREKEDF
>evm.model.Pn25.228
MHDWSDEACVQMVKKCKEAILAEDCRGKAIIVDMVLDPGCNDAMARENQYSLDLLNDTKEPSHFDGLYWQGEGRRRLKEDF
>evm.model.Pn4.3421
MNDQELHRYSQDAQPQLWNLLFSFINSMSLKCAVELGIPDIIHSHAQTPINMADLAASIPIPPNKTSHFRRLMRLLVHSNVFSVHKREDGDEGFLLTPMSRTLVTSNDNNGGNLSPFVIMMLHSSLVSPWHFLGQWLKGNDTQGTSFGMCHGAEVWDWANQCPDFNKKFNVAMVCDSQYLMKTFVKKCVTAFEGQRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPKDSRVKFVGGDMFHAIPPADAVLLKWILHDWNDEECIKILKNCKKAIPSKEEGGKVMILDMVVNSFPGDHMATEDQYFMDLLMMVNTSGMERDEKEWKKLFKDAGFTSYKITPVLGTRSLIELYP
>evm.model.Pn4.3404
MNDQELHRYSQDAQPQLWNLLFSFINSMSLKCAVELGIPDIIHSHAQTPINMADLAASIPIPPNKTSHFRRLMRLLVHSNVFSVHKREDGDEGFLLTPMSRTLVTSNDNNGGNLSPFVIMMLHSSLVSPWHFLGQWLKGNDTQGTSFGMCHGAEVWDWANQCPDFNKKFNMAMVCDSQYLMKTFVKKCVTAFEGQRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPKDSRVKFVGGDMFHAIPPADAVLLKWILHDWNDEECIKILKNCKKAIPSKEEGGKVMILDMVVNSFPGDHMATEDQYFMDLLMMVNTSGMERDEKEWKKLFKDAGFTSYKITPVLGTRSLIELYP
>evm.model.Pn4.3409
MADLAATIPIPPNKASHFRRLMRLLVHSNVFSVHKREDGDEGFLLTPMSRTLVASNNNLPPYVSMMLHSSLVSPWHFLGQWLKGNDTQGTPFGMRHGAETWDWANQCPDFNKKFNMAMRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPEDSRVKFVGGDMFHAVPPADAVLLKWILHDWNDEECIKILKNCKKAIPSKEEGGKVMILDMVVNSFPGDHMAIEDQYFMDLLMMVNTSGMERDEKE
>evm.model.Pn4.3411
MAMVCDSQYLMKTFVKKCVTAFEGQRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPEDSRVKFVGGDMFHAIPPADAVLLKWILHDWNDEDSIKILKNCKKAIPSKEEGGKVMILDMVVNSAPGDHNATEDEYFMDLLMMVLTSGMERDEKEWKKLFKDSGFTSYTITQVLGTRSLIELYP
>evm.model.Pn4.3296
MMLHSSLVSPWHFLGQWLKGNDTQGTSFGMCHGAEVWDWANECPDFNKKLNMAMFCDSQYLMKTFVKKCVTAFEGQRSLVDVGGGSGGAARLIAEAFPDIFHAIPHVVAGLPKDSRVKFVGGDMFHAIPPADAVLLKWILHDWYDEECIQILKNCKKAIPSKEEGGMVMILDMVVKSALGDHMAIEDEYFMDLWNGETRERMEEIV
>evm.model.Pn4.3393
MGEVQASQVSLPDAQTQLWNLLFSFINSMFLGCAVELGRADIKHSHDPTPNLLDLAASIPIPPNKTSHLRRLMRLLVHSGIFAVKNCDNGEEGFVLIPMSSLIVQGRDESILSSFVSMMLDSSLVSPWHFLGEWFEGKNLTTAFRLYRREEFWNKANQFPDQ
>evm.model.Pn4.3395
MNDQELHRCSQDAQPQLWNLLFSFINSMSLKCAVELGIPDIIHSHAQRPINMADLAASIPIPPNKTSQFRRLMRLLVHSNVFSVHKREDGDEGFLLTPMSRTLVTSNDNNGGNLSPFVSMMLHSSLVSPWHFLGQWLKGNDTQGTPFGMCHGAEVWDWANQCPDFNKKFNMAMRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPKDSRVKFVGGNMFHAIPPADAVLLKWILQDWNDEECIKILKNCKKAIPCKEEGGKVMILDMVVNSAPGDHMAIEDDFFMDLLMMVLASGMERDEKEWKKLFKDAGFTSYKITPVLGTRSLIELYP
>evm.model.Pn4.3362
MNDQELHRCSQDAQPQLWNLLFSFINSMSLKCAVELGIPDIIHSHAQRPINMADLAASIPIPPNKTSQFRRLMRLLVHSNVFSVHKREDGDEGSLLTPMSRTLVTSNDNNGGNLSPFVSMMLHSSLVSPWHFLGQWLKGNDTQGTPFGMCHGAEVWDWANQCPDFNKKFNMAMVCDSQYLMKTFVQKCVTAFEGQRSLVDVGGGSGGAARSIAEAFPDIQAVSVLDLPHVVAGLPKDSRVKFVGGNMFHAIPPADAVLLKWILQDWNDEECIKILKNCKKAIPCKEEGGKVMILDMVVNSAPGDHMAIEDDFFMDLLMMVLASGMERDEKEWKKLFKDAGFTSYKITPVLGTRSLIELYP
>evm.model.Pn4.3367
MNDQELHRYSQDAQPQLWNLLFSFINSMSLKCAVELGIPDIIHSHAQTPINMADLAASIPIPPNKTSHFRRLMRLLVHSNVFSVHKREDGDEGFLLTPMSRTLVTSNDNNGGNLSPFVIMMLHSSLVSPWHFLGQWLKGNDTQGTSFGMCHGAEVWDWANQCPDFNKKFNMAMVCDSQYLMKTFVKKCVTAFEGQRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPKDSRVKFVGGDMFHAIPPADAVLLKWILHDWNDEECIKILKNCKKAIPSKEEGGKVMILDMVVNSFPGDHMAIEDQYFMDLLMMVNTSGMERDEKEWKKLFKDAGFTSYKITPVLGTRSLIELYP
>evm.model.Pn4.3310
MGEVQASQVSLPDAQTQLWNLLFSFINSMFLGCAVELGRADIKHSHDPTPNLLDLAASIPIPPNKTSHLRRLMRLLVHSGIFAVKNCDNGEEGFVLIPMSSLIVQGRDESILSSFVSMMLDSSLVSPWHFLGEWFEGKNLTTAFRLYRREEFWNKANQFPDQ
MGRCWCGTGSAAKVIAEAFTCIKCTVLDPAPCGRRSAGDPRGGNILHDWSDEECVQILQRCKEAIPSREEGGKVILVELVAGAKPNDSTYMETIFYLDLLMAAHADGKEREEHELKKIFQDAGFSHYKITPALGLRSVIELFP
>evm.model.Pn37.3
MQHFLCAIVLLLIVQPSIFAYARNSHRDRKMLVDPLGSISNKNKSLKSSHPIPVIDDVLEPEDYPPPGPNRPLPPARNGACEEQRVMNTEETWHAESLLWNNFYSFVKSMALKCAIRLRIPEIINNHGPDFNKA
>evm.model.Pn25.230
MGHAKCNERVMNTEETWQAESLLWNNFYSFVKSMALKCAIRLRIPEIIHNHGRPAALHEVMAAVPIPAAKADPLRRLMRLLTDSGIFAIDQNGAYALTPASRLLLPGPGGTSLALSSLLSLEPDVVGVWDHLDEWYKVEDGAKTPFQVTYRMNTWDYCRSQPRLQGLLNDAMAADSEHLMDALVNNCGDVFRGLRSVVDAAGGVGMAAKTIARAFPEIEVTVLDQPHVVAGLPEKEDDSGVKFVGGDIFKSIPPANAVFLKWVLHDWTDEECIEILKKCKEAIPSKEAGGKVIIVDMVVDPSCSNGVARETQFAYDVFMLVVSMGKEREKEEWKKIFEDAGFSDCKFTPALGYRCVIEVYP
>evm.model.Pn25.237
MIRVLHDWTDEECVEILKKCKEAIPSKEARGKVIIVDMVVDPSCSNGVARETQFAYDVFMLVVSMGKEREKEDF
>evm.model.Pn25.228
MHDWSDEACVQMVKKCKEAILAEDCRGKAIIVDMVLDPGCNDAMARENQYSLDLLNDTKEPSHFDGLYWQGEGRRRLKEDF
>evm.model.Pn4.3421
MNDQELHRYSQDAQPQLWNLLFSFINSMSLKCAVELGIPDIIHSHAQTPINMADLAASIPIPPNKTSHFRRLMRLLVHSNVFSVHKREDGDEGFLLTPMSRTLVTSNDNNGGNLSPFVIMMLHSSLVSPWHFLGQWLKGNDTQGTSFGMCHGAEVWDWANQCPDFNKKFNVAMVCDSQYLMKTFVKKCVTAFEGQRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPKDSRVKFVGGDMFHAIPPADAVLLKWILHDWNDEECIKILKNCKKAIPSKEEGGKVMILDMVVNSFPGDHMATEDQYFMDLLMMVNTSGMERDEKEWKKLFKDAGFTSYKITPVLGTRSLIELYP
>evm.model.Pn4.3404
MNDQELHRYSQDAQPQLWNLLFSFINSMSLKCAVELGIPDIIHSHAQTPINMADLAASIPIPPNKTSHFRRLMRLLVHSNVFSVHKREDGDEGFLLTPMSRTLVTSNDNNGGNLSPFVIMMLHSSLVSPWHFLGQWLKGNDTQGTSFGMCHGAEVWDWANQCPDFNKKFNMAMVCDSQYLMKTFVKKCVTAFEGQRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPKDSRVKFVGGDMFHAIPPADAVLLKWILHDWNDEECIKILKNCKKAIPSKEEGGKVMILDMVVNSFPGDHMATEDQYFMDLLMMVNTSGMERDEKEWKKLFKDAGFTSYKITPVLGTRSLIELYP
>evm.model.Pn4.3409
MADLAATIPIPPNKASHFRRLMRLLVHSNVFSVHKREDGDEGFLLTPMSRTLVASNNNLPPYVSMMLHSSLVSPWHFLGQWLKGNDTQGTPFGMRHGAETWDWANQCPDFNKKFNMAMRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPEDSRVKFVGGDMFHAVPPADAVLLKWILHDWNDEECIKILKNCKKAIPSKEEGGKVMILDMVVNSFPGDHMAIEDQYFMDLLMMVNTSGMERDEKE
>evm.model.Pn4.3411
MAMVCDSQYLMKTFVKKCVTAFEGQRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPEDSRVKFVGGDMFHAIPPADAVLLKWILHDWNDEDSIKILKNCKKAIPSKEEGGKVMILDMVVNSAPGDHNATEDEYFMDLLMMVLTSGMERDEKEWKKLFKDSGFTSYTITQVLGTRSLIELYP
>evm.model.Pn4.3296
MMLHSSLVSPWHFLGQWLKGNDTQGTSFGMCHGAEVWDWANECPDFNKKLNMAMFCDSQYLMKTFVKKCVTAFEGQRSLVDVGGGSGGAARLIAEAFPDIFHAIPHVVAGLPKDSRVKFVGGDMFHAIPPADAVLLKWILHDWYDEECIQILKNCKKAIPSKEEGGMVMILDMVVKSALGDHMAIEDEYFMDLWNGETRERMEEIV
>evm.model.Pn4.3393
MGEVQASQVSLPDAQTQLWNLLFSFINSMFLGCAVELGRADIKHSHDPTPNLLDLAASIPIPPNKTSHLRRLMRLLVHSGIFAVKNCDNGEEGFVLIPMSSLIVQGRDESILSSFVSMMLDSSLVSPWHFLGEWFEGKNLTTAFRLYRREEFWNKANQFPDQ
>evm.model.Pn4.3395
MNDQELHRCSQDAQPQLWNLLFSFINSMSLKCAVELGIPDIIHSHAQRPINMADLAASIPIPPNKTSQFRRLMRLLVHSNVFSVHKREDGDEGFLLTPMSRTLVTSNDNNGGNLSPFVSMMLHSSLVSPWHFLGQWLKGNDTQGTPFGMCHGAEVWDWANQCPDFNKKFNMAMRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPKDSRVKFVGGNMFHAIPPADAVLLKWILQDWNDEECIKILKNCKKAIPCKEEGGKVMILDMVVNSAPGDHMAIEDDFFMDLLMMVLASGMERDEKEWKKLFKDAGFTSYKITPVLGTRSLIELYP
>evm.model.Pn4.3362
MNDQELHRCSQDAQPQLWNLLFSFINSMSLKCAVELGIPDIIHSHAQRPINMADLAASIPIPPNKTSQFRRLMRLLVHSNVFSVHKREDGDEGSLLTPMSRTLVTSNDNNGGNLSPFVSMMLHSSLVSPWHFLGQWLKGNDTQGTPFGMCHGAEVWDWANQCPDFNKKFNMAMVCDSQYLMKTFVQKCVTAFEGQRSLVDVGGGSGGAARSIAEAFPDIQAVSVLDLPHVVAGLPKDSRVKFVGGNMFHAIPPADAVLLKWILQDWNDEECIKILKNCKKAIPCKEEGGKVMILDMVVNSAPGDHMAIEDDFFMDLLMMVLASGMERDEKEWKKLFKDAGFTSYKITPVLGTRSLIELYP
>evm.model.Pn4.3367
MNDQELHRYSQDAQPQLWNLLFSFINSMSLKCAVELGIPDIIHSHAQTPINMADLAASIPIPPNKTSHFRRLMRLLVHSNVFSVHKREDGDEGFLLTPMSRTLVTSNDNNGGNLSPFVIMMLHSSLVSPWHFLGQWLKGNDTQGTSFGMCHGAEVWDWANQCPDFNKKFNMAMVCDSQYLMKTFVKKCVTAFEGQRSLVDVGGGSGGAARSIAEAFPDIQVVSVLDLPHVVAGLPKDSRVKFVGGDMFHAIPPADAVLLKWILHDWNDEECIKILKNCKKAIPSKEEGGKVMILDMVVNSFPGDHMAIEDQYFMDLLMMVNTSGMERDEKEWKKLFKDAGFTSYKITPVLGTRSLIELYP
>evm.model.Pn4.3310
MGEVQASQVSLPDAQTQLWNLLFSFINSMFLGCAVELGRADIKHSHDPTPNLLDLAASIPIPPNKTSHLRRLMRLLVHSGIFAVKNCDNGEEGFVLIPMSSLIVQGRDESILSSFVSMMLDSSLVSPWHFLGEWFEGKNLTTAFRLYRREEFWNKANQFPDQ
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University