Enzyme
Requires Fe2+ and ascorbate. This plant enzyme catalyses an early step in the flavonoid biosynthesis pathway, leading to the production of flavanols and anthocyanins. Substrates include (2S)-naringenin, (2S)-eriodictyol, (2S)-dihydrotricetin and (2S)-pinocembrin. Some enzymes are bifuctional and also catalyse EC 1.14.20.6, flavonol synthase.
F3H
Enzyme
Flavanone 3-hydroxylase
Enzyme name
EC:1.14.11.9
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Class
(2S)-flavan-4-one,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Sysname
a (2S)-flavan-4-one + 2-oxoglutarate + O2 = a (2R,3R)-dihydroflavonol + succinate + CO2 [RN:R07329]
Reaction
(2R,3R)-dihydroflavonol [CPD:C21417];succinate [CPD:C00042];CO2 [CPD:C00011]
Product
Species information
Piper nigrum
Species name
Piper nigrum is a species of flowering plant in the family Piperaceae (pepper family).
Species info
Gene sequence info
Piper_nigrum.F3H.fa
Download
>evm.model.Pn1.3607
MPSLPLDNQSLPESFVQDKDDRPQVPHDAFSDQIQLVSLAGTDDAGEGRKEGRKKIVEACEKWGVFQVVEHGVDQGLMDEMVRLSKEFFALPAQVVAMSGDRGEAISFPALTTDYKQWPETPNGWRKVMEDYAEKLMGLSFKLLELLSEAMNLKKDAIAKACLAIDQKVIVNFYRSCPYTDLTFGLSRHTDPGTITILLQDQVGGLQVTCDHGKTWIDVQPLHGAFVVNLGNHGSVTISRLPFII
>evm.model.Pn3.2598
MAIHVMPSLPLESQSLPASFVQDKDDRPQVPHDAFSDEVPVVSLAGIDDAGEGRINARKKIVEACEKWGVFQAVDHGVDQGLVDEMVRLSKEFFALPAQEKLEVAMSGDRRGGYLVSSTHQESKAFDWREYMMFFTYPVEARDYKQWPESPNEWRQVMEDYAEKLTGLSYKLLELLSEAMNLKKDAIAKACLDIDQKVIVNFYPRCPQPDLTFGLSRHTDPGLITILLQDQVGGLQVTCDHGKTWIPVRPLHGAFVVNLGDYGSVPSNGRFKSCDHRAVVNSSCNRISVATFLYPSQEAMVYPLGLHEEEKSIIEKGVTFKEMYKKKMNDGRIVQV
>evm.model.Pn3.2596
MAIHVMPSLPLESQSLPASFVQDKDDRPQVPHDAFIDEVPVVSLAGIDDAGEGRINARKKIVEACEKWGVFQAVDHGVDQGLVDEMVRLSKEFFALPAQEKLEVAMSGDRRGGYLVSSTHQERKAFDWREFMMFFTYPVEARDYKQWPESPNEWRKVMEDYAEKLTGLSYKLLELLSEAMNLKKDAIAKACLDIDQKVIANFYPRCPQPDLTFGLSRHTDPGLITILLQDQVGGLQVTCDHGKTWIPVRPLHGAFVVNLGDYGSVLSNGRFKSCDHRAVVNSSCNRISVATFLYPSQEAMVYPLGLHEEEKSIIEKGVTFKEMYKKKMNDGRIVQV
>evm.model.Pn4.999
MAHATTLPFLPSETQEKTLRESFVRDEDEQPKVAYNVFSDDIPVISLAGIDDPDGRADVCRKIVEACEDWGIFQVVHHGVDGDLLREMMRLAREFFAMPAEEKLRYDMSGGKKGGFVVSSHLQGETVKDWRELVTYFSYPTRSRDYERWPDKPEGWRKLTEEYCEKAMGLACKLLAVLSEAMGLESEALTKACIEMDQKVVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDHVGGLQATRDGGRTWITIQPIDGAFVVNLGDHGHYLSNGRFKNADHQAVVNSDCNRLSIATFQNPAPEAVVYPLAVRDGDKPIMEEAITFSEMYRRKMSRDLELARLKKLAKNTPEEMEKTRIEFESKSKPLSEILA
>evm.model.Pn4.998
MEDYSQQLMGLAQKLLQLLSEAISLAKDAIARACLALDQKVIAYPRSAASRTWITVEPVYGAFVNLSNGMFKSADYRAVVNGNSSRFSVAAFQYPLHVSMLYPLAIRSGEESIMEEPIAFKEKYARKMDEARAKQGR
>evm.model.Pn22.834
MAPTVQPIPLDGQTLPESFIRSEADRPKVAYNAFSNEIPVISLANVDDEDDGVVGGGGQGRRAEARRKIAEACEEWGVFQVVDHGVDQVLMDEVLRLSKEFFALPAEEKLKFDMTGGKRGGYLVSSHIQGMKTQDWREFLTYFTYPVETRDYTLWPEKPQGLTKAMEDYSQQLMGLAQKLLQLLSEAMGLAKDAIARACMALDQKVIANFYPKCPQPDLTFGLCRHTDPGTITILLQDQVGGLQVTRDGAKTWITVEPIHGAFVVNLGDHGHYLSNGRFKSADHRAVVNGNSSRFSVATFQYPLQESILYPLAIISGEESIMEEPITFKEMYARKMDEARAKNGR
>evm.model.Pn22.675
MAPTVQPIPLDGQTLPESFIRSEADRPKVAYNAFSNEIPVISLANVDDEDDGVVGGGGQGRRAEARRKIAEACEEWGVFQVVDHGVDQVLMDEVLRLSKEFFALPAEEKLKFDMTGGKRGGYLVSSHIQGMKTQDWREFLTYFTYPVETRDYTLWPEKPQGLTKAMEDYSQQLMGLAQKLLQLLSEAMGLAKDAIARACMALDQKVIANFYPKCPQPDLTFGLGRHTDPGTITILLQDQVGGLQVSRDGAKTWITVEPIHGAFVVNLGDHGHYLSNGRFKSADHRAVVNGNSSRFSVATFQYPLQESILYPLAIISGEESIMEEPITFKEMYARKMDEARAKNGR
MPSLPLDNQSLPESFVQDKDDRPQVPHDAFSDQIQLVSLAGTDDAGEGRKEGRKKIVEACEKWGVFQVVEHGVDQGLMDEMVRLSKEFFALPAQVVAMSGDRGEAISFPALTTDYKQWPETPNGWRKVMEDYAEKLMGLSFKLLELLSEAMNLKKDAIAKACLAIDQKVIVNFYRSCPYTDLTFGLSRHTDPGTITILLQDQVGGLQVTCDHGKTWIDVQPLHGAFVVNLGNHGSVTISRLPFII
>evm.model.Pn3.2598
MAIHVMPSLPLESQSLPASFVQDKDDRPQVPHDAFSDEVPVVSLAGIDDAGEGRINARKKIVEACEKWGVFQAVDHGVDQGLVDEMVRLSKEFFALPAQEKLEVAMSGDRRGGYLVSSTHQESKAFDWREYMMFFTYPVEARDYKQWPESPNEWRQVMEDYAEKLTGLSYKLLELLSEAMNLKKDAIAKACLDIDQKVIVNFYPRCPQPDLTFGLSRHTDPGLITILLQDQVGGLQVTCDHGKTWIPVRPLHGAFVVNLGDYGSVPSNGRFKSCDHRAVVNSSCNRISVATFLYPSQEAMVYPLGLHEEEKSIIEKGVTFKEMYKKKMNDGRIVQV
>evm.model.Pn3.2596
MAIHVMPSLPLESQSLPASFVQDKDDRPQVPHDAFIDEVPVVSLAGIDDAGEGRINARKKIVEACEKWGVFQAVDHGVDQGLVDEMVRLSKEFFALPAQEKLEVAMSGDRRGGYLVSSTHQERKAFDWREFMMFFTYPVEARDYKQWPESPNEWRKVMEDYAEKLTGLSYKLLELLSEAMNLKKDAIAKACLDIDQKVIANFYPRCPQPDLTFGLSRHTDPGLITILLQDQVGGLQVTCDHGKTWIPVRPLHGAFVVNLGDYGSVLSNGRFKSCDHRAVVNSSCNRISVATFLYPSQEAMVYPLGLHEEEKSIIEKGVTFKEMYKKKMNDGRIVQV
>evm.model.Pn4.999
MAHATTLPFLPSETQEKTLRESFVRDEDEQPKVAYNVFSDDIPVISLAGIDDPDGRADVCRKIVEACEDWGIFQVVHHGVDGDLLREMMRLAREFFAMPAEEKLRYDMSGGKKGGFVVSSHLQGETVKDWRELVTYFSYPTRSRDYERWPDKPEGWRKLTEEYCEKAMGLACKLLAVLSEAMGLESEALTKACIEMDQKVVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDHVGGLQATRDGGRTWITIQPIDGAFVVNLGDHGHYLSNGRFKNADHQAVVNSDCNRLSIATFQNPAPEAVVYPLAVRDGDKPIMEEAITFSEMYRRKMSRDLELARLKKLAKNTPEEMEKTRIEFESKSKPLSEILA
>evm.model.Pn4.998
MEDYSQQLMGLAQKLLQLLSEAISLAKDAIARACLALDQKVIAYPRSAASRTWITVEPVYGAFVNLSNGMFKSADYRAVVNGNSSRFSVAAFQYPLHVSMLYPLAIRSGEESIMEEPIAFKEKYARKMDEARAKQGR
>evm.model.Pn22.834
MAPTVQPIPLDGQTLPESFIRSEADRPKVAYNAFSNEIPVISLANVDDEDDGVVGGGGQGRRAEARRKIAEACEEWGVFQVVDHGVDQVLMDEVLRLSKEFFALPAEEKLKFDMTGGKRGGYLVSSHIQGMKTQDWREFLTYFTYPVETRDYTLWPEKPQGLTKAMEDYSQQLMGLAQKLLQLLSEAMGLAKDAIARACMALDQKVIANFYPKCPQPDLTFGLCRHTDPGTITILLQDQVGGLQVTRDGAKTWITVEPIHGAFVVNLGDHGHYLSNGRFKSADHRAVVNGNSSRFSVATFQYPLQESILYPLAIISGEESIMEEPITFKEMYARKMDEARAKNGR
>evm.model.Pn22.675
MAPTVQPIPLDGQTLPESFIRSEADRPKVAYNAFSNEIPVISLANVDDEDDGVVGGGGQGRRAEARRKIAEACEEWGVFQVVDHGVDQVLMDEVLRLSKEFFALPAEEKLKFDMTGGKRGGYLVSSHIQGMKTQDWREFLTYFTYPVETRDYTLWPEKPQGLTKAMEDYSQQLMGLAQKLLQLLSEAMGLAKDAIARACMALDQKVIANFYPKCPQPDLTFGLGRHTDPGTITILLQDQVGGLQVSRDGAKTWITVEPIHGAFVVNLGDHGHYLSNGRFKSADHRAVVNGNSSRFSVATFQYPLQESILYPLAIISGEESIMEEPITFKEMYARKMDEARAKNGR
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University