Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Pinus lambertiana
Species name
Sugar pine (Pinus lambertiana) is a species of seed plant in the family Pinaceae (pine family).
Species info
Gene sequence info
Pinus_lambertiana.FLS.fa
Download
>PILA_02149
MVNICREIPPCVVAFALKLWLEPQVWGARWQDREAKSWAWCCMCLWDPGNIVFEVQCAAVEAISALSAMRKKPNWACFLRRKRSHTVAFFLEPGPDCLVECLPTCHSESNPPKYSPVKYGDYLAQRFKDTSSESSSY
>PILA_03383
MISSLFGCLMTSDEWNLILAEVNDEYGRKLLGVVNKLLAALSMSLGMKESAMKEAVGGENLEMEMKINYYPPCPQPELALGVEPHTDMSALTLLIPNEVPGLQVFNDGHWVSATYVPNAIIVHIGDQLEILSNGRYKSVLHRSVVLKSLREGGQSMWK
>PILA_03592
MSGLNDERRYKTMKEISDTCSKWGFFQVINHGIPSQILTTAMNVGREFFNTPLEEKQKYANDAITYEGYGSRIGVQKGALIDWGDYDFHHFLPPSIRNESKWPSKPENYRMTYEEIMEMLMRDRDERILLKCGRPLPEVTFSILCQHRSSLGLSEGSIWRKGGNGHVHSNKLLSSMSRTRSDFGFVISL
>PILA_06868
MTLVNGYQPKRKQKPQITCYLRESPRCSQVRASVDWKPRPQQAESWRRENRREVTSPQENMSSASVCQAVERPRYTSVREKRTQLSHGELNINRNPDLETLNRLASYTCLHHHNWHGDEQLGGVGTIKFRGPLTVLKPNDVPGLQISRNNAWVTAKPILNTLIIHIGDQLQTLSNGRYKSVLHRTLVSKDKVRMSWPVFCIPPLDLVVGPLKQLIDEKHPPLFDAMTFREFKQRKKTKANQKTS
>PILA_08507
MLKYREVTEEYGRQVLGVVDKLLSAFSINLRLEESTLRNAVGGYNLEMELKINYYPPCPQSDMAQGVLPHTDMSALTVLKPNDVPWLQILRNNEWLTAKHVPNTLIIHIGDQLQTLSNGRYKRKDKVRMSWPVFCIPPSWPAQTVDR
>PILA_14695
MMEPDARNEKRWSRHSQNRVGILMMKSTGIESRDYGHGVCYEHGHGHGHGHGGDAIREVTEEYGRLVLGVVDKLLSAFSINLGLEESTVRNALGGENLEMELKINYYPPCPQPDMALEVLPYTDMSALTVLKPNDVPGLQILRNNEWVTAKYVPNTLIIHIGDQLQTLSNGRSKSVLHRTLVSKDKLRMSWTRGRSSPFLPLI
>PILA_14759
MAAMGSWAITRAQNLAESGILTVPPEYVRQVEKALVVQEADDPRFLVPVIDLRRFSLLPRDHLCKDQYDTISAQISRAAENWGFFQIRNHGIPDSLIARVQAASRAFFQLPAEEKEAYANEAHNPIGYGSKLGYSADGEVKLEWGDYYYNLMRPLDRRDMSKWPIQLSDFTEAMDEYSTELSKLFEYLMKVLSRDLGLEGENSLNESVGGERKEQQIRINYYPPCPQPDLVVGVAPHSDPAAITILLHDQIPGLQIRKDGAWIDVQFVPGALVVNIGDQLEILSNGKYSLKDVMGRVLHSSV
>PILA_21254
MENWEFKRVQTLADSGIQTVPPQYVRPVEKTHSDPNSYQVPIIDLQLAFSPQKHDQICKDQYDSIAAQISRAAENWGFFQIINHGIPDSLIARVQATGKTFFQLPAEEKEAYANEAQNPIGYGSKIGYSPDSEAKLDWGDYYYNAIWPPHRRDMNKWLTKLSDFTESMDEYSIGLTKLFELVMEVLSRDLGLETENSLNESVGGEKKELYIRMNYYPPCPQPDLVAGLAAHSDLNVLTILLHDQTPGLQIRKNGAWIDAQCIPGALVVNVADQMEILSNGKYKSIEHRTRVHKDRSRMSWAVFCSPPRDMVVSPRKELIDEQHPPLYQGASFREYVTKAFKKGLVGKGYIQDAKQQHSLVSDS
>PILA_21852
MVDPRSLNSDKCYGGCSRSVHPPAGRLLALDLPVIGRCAGSEAGVMTSLAVCLFPGPFPLFTSIDRVLELSAKLSLGGACPLAHSATNRFDSEDDWSAPMVARVYLITPLLEFLDLPLEEKWEQRPVRPGLHMLEGYGRFFDVSDDTVLDWFDALVHYISPEWAKAIEHWPKNPSTYRETYEKYGAEIMGLMEKLLGLLSEGLGLDPGAVSINTSSSGLKNITIDMRLFFVFDTARCSALLYFPLLIICHYTIPISFYKNSAKV
>PILA_21853
MEDTDQRFKIVSQIAQASQEWGFFQIINHGIPLSLLETVKRVSQEFFDLPLEEKREKCPVRPGLHMLEGYGHLFDISDDTVLDWVVSLVHYISPEWAKALEHWPKKPSTYRYNSIHTL
>PILA_35420
MASARVQAVSLSGFQSIPSEFVRPVYERPARETFNEFEIPLIDLSSLQVDALREKTVAEIGRASQEWGIFQVVNHGISDELVECLQAAGRVFFDLPLEEKEAYANLEGVTDDRKLKCTSDGRQGWSDFYFHMVWPPSLIDFNKWPKHPPFYREVTEEYGRQVLGVVDKLLSSFSINLGLEESTVRKALGGGNLEMELKINYYPLCPQPDMALGVLPHTDMSALTVLKPNDVPGLQRNNEWVTAKYVPNTLIIHIGDQLQTLSNGRYQSVLHRTLVSKDKVRMSWPVFCIPPLDLVVGPLKQLIDENHPPLFDAMTYREFKRRKKTKAKNQSS
>PILA_36626
MAVRVQAIAEGGLESIPPQFVRPEHERPAALTTFSMNYNDWGQPACEIPVIDLSNLDLSGHVREKTLKEIASASWEWGIFQIVNHGISEELIQRLQSVGEEFFRLPQEEKEAYANNPAAGNLEGYGTKLAANLNGKLEWLDYYFHMLWPPSRRDFSTWPKHPPSYIEVNDEYGRKLLGVVNKLLAALSMSLGMEESAIKEAVGGENLEMEMKINYYPPCPQPELALGVEPHTDMSALTLLIPSEVPGLQVFNDGHWVSATYIPNAIIIHIGDQLEVGQPSTVPPGLPPVPSWAEDDSQNYPPPS
>PILA_37486
MAVRVQAIAEGGLESIPHQFIRPEHERPAALTTFSMNSTGSGQPSCDLPVIDLSNLDLSGHVREKTLQEIASASREWGIFQIVNHGISEGLVQRLQSVGEEFFHLPQEEKESYANNPAAGNLEGYGTKLAANLDGKLEWLDYYFHMLWPPSRRDFSSWPKHPPSYIEVNDEYGRKLLGVVNKLLAALSMSLGMEESAMKEALGGENLEMEMKINYYPPCPQPELALGVEPHTDMSALTLLIPNDVPGLQVFNDGHWVSATYIPNAIIVHIGDQLEILSNGRYKSVLHRSVVSKDRVRMQCSAAHLLTPSSAP
>PILA_37866
MASVYESVDMSLPKWGKCFLTVESVQELVKKQKETIPERYIRRDHEQPTPNSMSNHQLDVPIIDMAKLSEGQQREGEIKKIAKACEDWGFFQVVNHGIPNSLIDGAKRVGKEFSQLPLEEKQKYALKAGSLEGYG
MVNICREIPPCVVAFALKLWLEPQVWGARWQDREAKSWAWCCMCLWDPGNIVFEVQCAAVEAISALSAMRKKPNWACFLRRKRSHTVAFFLEPGPDCLVECLPTCHSESNPPKYSPVKYGDYLAQRFKDTSSESSSY
>PILA_03383
MISSLFGCLMTSDEWNLILAEVNDEYGRKLLGVVNKLLAALSMSLGMKESAMKEAVGGENLEMEMKINYYPPCPQPELALGVEPHTDMSALTLLIPNEVPGLQVFNDGHWVSATYVPNAIIVHIGDQLEILSNGRYKSVLHRSVVLKSLREGGQSMWK
>PILA_03592
MSGLNDERRYKTMKEISDTCSKWGFFQVINHGIPSQILTTAMNVGREFFNTPLEEKQKYANDAITYEGYGSRIGVQKGALIDWGDYDFHHFLPPSIRNESKWPSKPENYRMTYEEIMEMLMRDRDERILLKCGRPLPEVTFSILCQHRSSLGLSEGSIWRKGGNGHVHSNKLLSSMSRTRSDFGFVISL
>PILA_06868
MTLVNGYQPKRKQKPQITCYLRESPRCSQVRASVDWKPRPQQAESWRRENRREVTSPQENMSSASVCQAVERPRYTSVREKRTQLSHGELNINRNPDLETLNRLASYTCLHHHNWHGDEQLGGVGTIKFRGPLTVLKPNDVPGLQISRNNAWVTAKPILNTLIIHIGDQLQTLSNGRYKSVLHRTLVSKDKVRMSWPVFCIPPLDLVVGPLKQLIDEKHPPLFDAMTFREFKQRKKTKANQKTS
>PILA_08507
MLKYREVTEEYGRQVLGVVDKLLSAFSINLRLEESTLRNAVGGYNLEMELKINYYPPCPQSDMAQGVLPHTDMSALTVLKPNDVPWLQILRNNEWLTAKHVPNTLIIHIGDQLQTLSNGRYKRKDKVRMSWPVFCIPPSWPAQTVDR
>PILA_14695
MMEPDARNEKRWSRHSQNRVGILMMKSTGIESRDYGHGVCYEHGHGHGHGHGGDAIREVTEEYGRLVLGVVDKLLSAFSINLGLEESTVRNALGGENLEMELKINYYPPCPQPDMALEVLPYTDMSALTVLKPNDVPGLQILRNNEWVTAKYVPNTLIIHIGDQLQTLSNGRSKSVLHRTLVSKDKLRMSWTRGRSSPFLPLI
>PILA_14759
MAAMGSWAITRAQNLAESGILTVPPEYVRQVEKALVVQEADDPRFLVPVIDLRRFSLLPRDHLCKDQYDTISAQISRAAENWGFFQIRNHGIPDSLIARVQAASRAFFQLPAEEKEAYANEAHNPIGYGSKLGYSADGEVKLEWGDYYYNLMRPLDRRDMSKWPIQLSDFTEAMDEYSTELSKLFEYLMKVLSRDLGLEGENSLNESVGGERKEQQIRINYYPPCPQPDLVVGVAPHSDPAAITILLHDQIPGLQIRKDGAWIDVQFVPGALVVNIGDQLEILSNGKYSLKDVMGRVLHSSV
>PILA_21254
MENWEFKRVQTLADSGIQTVPPQYVRPVEKTHSDPNSYQVPIIDLQLAFSPQKHDQICKDQYDSIAAQISRAAENWGFFQIINHGIPDSLIARVQATGKTFFQLPAEEKEAYANEAQNPIGYGSKIGYSPDSEAKLDWGDYYYNAIWPPHRRDMNKWLTKLSDFTESMDEYSIGLTKLFELVMEVLSRDLGLETENSLNESVGGEKKELYIRMNYYPPCPQPDLVAGLAAHSDLNVLTILLHDQTPGLQIRKNGAWIDAQCIPGALVVNVADQMEILSNGKYKSIEHRTRVHKDRSRMSWAVFCSPPRDMVVSPRKELIDEQHPPLYQGASFREYVTKAFKKGLVGKGYIQDAKQQHSLVSDS
>PILA_21852
MVDPRSLNSDKCYGGCSRSVHPPAGRLLALDLPVIGRCAGSEAGVMTSLAVCLFPGPFPLFTSIDRVLELSAKLSLGGACPLAHSATNRFDSEDDWSAPMVARVYLITPLLEFLDLPLEEKWEQRPVRPGLHMLEGYGRFFDVSDDTVLDWFDALVHYISPEWAKAIEHWPKNPSTYRETYEKYGAEIMGLMEKLLGLLSEGLGLDPGAVSINTSSSGLKNITIDMRLFFVFDTARCSALLYFPLLIICHYTIPISFYKNSAKV
>PILA_21853
MEDTDQRFKIVSQIAQASQEWGFFQIINHGIPLSLLETVKRVSQEFFDLPLEEKREKCPVRPGLHMLEGYGHLFDISDDTVLDWVVSLVHYISPEWAKALEHWPKKPSTYRYNSIHTL
>PILA_35420
MASARVQAVSLSGFQSIPSEFVRPVYERPARETFNEFEIPLIDLSSLQVDALREKTVAEIGRASQEWGIFQVVNHGISDELVECLQAAGRVFFDLPLEEKEAYANLEGVTDDRKLKCTSDGRQGWSDFYFHMVWPPSLIDFNKWPKHPPFYREVTEEYGRQVLGVVDKLLSSFSINLGLEESTVRKALGGGNLEMELKINYYPLCPQPDMALGVLPHTDMSALTVLKPNDVPGLQRNNEWVTAKYVPNTLIIHIGDQLQTLSNGRYQSVLHRTLVSKDKVRMSWPVFCIPPLDLVVGPLKQLIDENHPPLFDAMTYREFKRRKKTKAKNQSS
>PILA_36626
MAVRVQAIAEGGLESIPPQFVRPEHERPAALTTFSMNYNDWGQPACEIPVIDLSNLDLSGHVREKTLKEIASASWEWGIFQIVNHGISEELIQRLQSVGEEFFRLPQEEKEAYANNPAAGNLEGYGTKLAANLNGKLEWLDYYFHMLWPPSRRDFSTWPKHPPSYIEVNDEYGRKLLGVVNKLLAALSMSLGMEESAIKEAVGGENLEMEMKINYYPPCPQPELALGVEPHTDMSALTLLIPSEVPGLQVFNDGHWVSATYIPNAIIIHIGDQLEVGQPSTVPPGLPPVPSWAEDDSQNYPPPS
>PILA_37486
MAVRVQAIAEGGLESIPHQFIRPEHERPAALTTFSMNSTGSGQPSCDLPVIDLSNLDLSGHVREKTLQEIASASREWGIFQIVNHGISEGLVQRLQSVGEEFFHLPQEEKESYANNPAAGNLEGYGTKLAANLDGKLEWLDYYFHMLWPPSRRDFSSWPKHPPSYIEVNDEYGRKLLGVVNKLLAALSMSLGMEESAMKEALGGENLEMEMKINYYPPCPQPELALGVEPHTDMSALTLLIPNDVPGLQVFNDGHWVSATYIPNAIIVHIGDQLEILSNGRYKSVLHRSVVSKDRVRMQCSAAHLLTPSSAP
>PILA_37866
MASVYESVDMSLPKWGKCFLTVESVQELVKKQKETIPERYIRRDHEQPTPNSMSNHQLDVPIIDMAKLSEGQQREGEIKKIAKACEDWGFFQVVNHGIPNSLIDGAKRVGKEFSQLPLEEKQKYALKAGSLEGYG
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University