Enzyme

Requires Fe2+ and ascorbate. This plant enzyme catalyses an early step in the flavonoid biosynthesis pathway, leading to the production of flavanols and anthocyanins. Substrates include (2S)-naringenin, (2S)-eriodictyol, (2S)-dihydrotricetin and (2S)-pinocembrin. Some enzymes are bifuctional and also catalyse EC 1.14.20.6, flavonol synthase.

F3H

Enzyme

Flavanone 3-hydroxylase

Enzyme name

EC:1.14.11.9

Entry

Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

Class

(2S)-flavan-4-one,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)

Sysname

a (2S)-flavan-4-one + 2-oxoglutarate + O2 = a (2R,3R)-dihydroflavonol + succinate + CO2 [RN:R07329]

Reaction

(2R,3R)-dihydroflavonol [CPD:C21417];succinate [CPD:C00042];CO2 [CPD:C00011]

Product

Species information

Pinus lambertiana

Species name

Sugar pine (Pinus lambertiana) is a species of seed plant in the family Pinaceae (pine family).

Species info

Gene sequence info

Pinus_lambertiana.F3H.fa

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>PILA_00340
MNYYPPCPHPDLTLGLKRHTELGTLTILLQDKVGGLQATKDDGKTWVTVEPIDGAFVVNLGDQMHVLSNGIFKSADHQAVVNSSALRQSIVTFYNPHPDSIVYPLDELVPVSEQESTARAKFERYIYREFYQKKMIKHLLDREMKRNKGIILPQQQKG
>PILA_03812
MAPAADVVASERPDFLVTAEPPKSLQSIFIRDEDERPKVPYNQFSKDVPVISLSGIAGEERGRVRDEVSKACSEWGIFQVVDHGVPQELVESMTQLSREFFALSPEEKLKYDMRGGKREGFVVSSHLQGESVQDWREIFIYFSYPLHQRDYSMWPVKPEGWREIVEKYSEFLMGLASKLLGIISEALGLETEAVTNACVEMDQKVVVNFYPKCPEPDMTLGLKRHTDPGTITLLLQDQVGGLQATKDDGKNWITMEPIKGAFVVNLGDHMHYLSNGKFKWRENVHAWLSEEHVMEQINREHRSLVSTAYKFLFVHGYINFGVAPAIKSGVPEEATKVSVVVIGAGLAGLAAARQLLLHGCKVVVVEGKSRPGGRVFTHIMERKGQVAAADLGGSVITRIPGNPLAVLSRQLCLPLHKVRDKCPLYQPDGRPVDAEIDSKLYGVAGKIEERQLLNWHFSNLEYANAGLLSELCLAFWDQDDPYEMGGDHCLLAGGNCPRSSVSSRYGSLYSSPRGLKEWFDQLCSGVTWKQSRCY
>PILA_04575
MDWRELYVTPCSPALNVQHWSTKPVQFRETLSKYSEEMIKLAKTLQGLISEFLGVEADAIQKRCGDGEQKVLMNYYLPCPRPDLTLGLKRHTDPGTLTILLQDKVGGLQATKDDGKTWVTVEPIDGAFVVNLGDQMHVHSTHGSQPLHTTSTVSQSTTRPVALRHTSQRAKFQFFCTMDRLILNAFITVSLQEEAPSSKPTDEPAPQPPPVHDASQGLKGDDNAQE
>PILA_04579
MIKLAKTLQGLISESLGVEADAIQKRCGEGEQKVLMNYYPPCPRPDLTLGLKRHTDPGTLTILLQDKVGGVQATKDDGKTWVTVEPIDGAFVVNLGDQMHVLSNGIFKSADHQAVVNSSALRQSIVTFYNPHIYISKCWSAFIYIYIYGIKVHSTHGSQPLHTTSTSQLVSQSATPPVALRHTSQRAEFQFFCNMDHPVLNAFITVSLQEEAHSSKPTDEPAPQPPPVHDALQGLKRDDNAQE
>PILA_05102
MRCVEDFFSLPVEERMQYTIEAGEFVGYGNGSFINDDPLMDWRELYVIPCSPALNVQHWSTKPVQFRETLSKYSEEMIKLAKTLQGLISESLGVEADAIQKRCGEGEDYYPPCPRPDLTLGLKRHTDPGTLTILLEDKVGGLQATKDDGKTWVTMEPIDGSFVVNLGDQMHVSVLSNGIFKSADHQAVVNSSALRQSIVTFYNPHPDSIVYPLDELVLVSEQESPAREKFERYIYREFYQKKMIKHLLDREMKRNKGIILPQQQKG
>PILA_06616
MIKLAKTLLGLISESLGVEADAIQKRCGEGEQKVLMNYYPPCPRPDLTLGLNRHTDPGTLTILLQDKVGGLQATKDDGKTWVTVEPIDGAFVVNLGDQMHVSVLSNGIFKSADHQAVVNSSALRQSIVTFYNPHPDSIVYPLDELVLVSEQESLARAKFERYIYREFYQKKMIKHLLDREMKRNKGIILP
>PILA_12680
MAPPSLAGVSSLPEATGSATDGNIDWNFQMAPMLIPRDLRVSHNKARMQIACHLTLNLMLITWRRPIMGIIRNIRIKPIMSRETLSKYSEEMIKLAKTLQGLISESLGVEADAIQKRCGEGEQKVLMNYYPPCPRPDLTLGLKRHTDPGTLTILLQDKVGGLQATKDDGKTWVTVEPIDGAFVVNLGDQMHVLSNGIFKSADHQAVVNSSALRQSIVTFYNPHPDSIVCPLDELVLVSEQESPARAKFERYIYREFYQKKMIKHLLDREMKRNKGIILPQQKKG
>PILA_12855
MAPAAVVASDRPDFLVTAEEPKSLQSIFVRDEDERPKVAYNQFSKDIPVISLSGIAGEERGRVKDEVSKACSEWGIFQVVDHGVPQELVDSMTQLSRAFFALPAEEKLKYDMRGGKRGGFVVSSHLQGEAVLDWREICTYFSYPLHQRDYTRWPVKPDGWREIVEKYSESLMGLACNLLGIISEALGLETEAVTKACVEMDQKVVINFYPKCPEPDMTLGLKRHTDPGTITLLLQDQVGGLQATKDDGKNWITVEPIQGAFVVNLGDHMHYLSNGKFKTADHQAVVNSNSSRLSIATFQNPAQEGIVYPLDGVVKEAEGEKCFMEEPITRSDVLQKDEPRHRARSPEEDG
>PILA_13997
MEPIKGAFVVNLGDHMHYLSNGKFKCADHQAVVNSNSSKLSIATFQNPAQEAIVYPLDGVVKEAEGEKFLQVSKKAKPEKSWRVKVEKKAQCVEVQAAMSVGGVVVASVDALVAAQYVEKAVSMGVDRQQLASLLTKFQL
>PILA_17793
MAGANIENSVNLPGLGDVNASTLLDVLKLSENFNVDVQIIKPAVRDCKKNAATRDADQSQQKEFELDASIIAQSIFAPPNFHVNRQMASVVTNQAAERNAPIIHPLPVSSRTPKEINILGQAPREDQASSFPSSTKDSAHSEELSPPHKDFTPPDESELRSAPFDAKDINVFTAPVQACLFLDEHNMQARLFPSPTPLPGCILRMGYMSRFFHREIVEKYSEFLMGLASKLLGIISEALGLETEAVTNACVEMDQKVVVNFYPKCPEPDMTLGLKRHTDPGTITLLLQDQVGGLQATKDDGKNWITVEPIKGAFVVNLGDHMHGWWIGNVGNTEQGWCYWHYGFHTGYFLPKLLRNSLEMVVELQFKQKVEQTNPFGSTCVDVYQNITFQVMKKDHITTVTLFPS
>PILA_20889
MIKLAKTLQGLISESLGVEADAIQKRCGEGEQNVLMNYYRPCPCPDLTLGLKRHTDPGTLTILLQDKVLSNGIFKSADHQAIVNSSALRQSIVTFYNPHPDSIVYPLDELVLVSEQEIPARANFELIKMAPTALEVKTVKSLADLGIKDLPPNYVKEEDERPVVAYNDFSHEVPVISLAKARGGGAEREETNTHELLSLLNDRETLSNYSEEMIKLAKTLQGLISESLGVEADAIQKRCGEGEQKVLMNYYPPCPRPDLTLGLKRHTDPGTLTILLQDKVGGLQATKDDGKTWVTVEPIDGAFIVNLGDQMHVSVRVLLYSLICLVLLYLLILDLENILESNGKERKKLLFFYVLNNGIFKSADHQAVVNASALRQSIVTFYNPHPDSIVYPLDELVLVSEQESPARAKFERYIYREFYQKKMIKHLLDREMKRNKGIILPQQQKG
>PILA_22907
MTLGLKRHTDSGTITLLLQDQVGGLQATKDDGKNWITVEPIKGAFVVNLGDHMHYLSNGKFKCADHQAVVNSNSSRLSIATFQNPAQEAIVYPLDGVVKEAEGEKCFMEEPITFAQMYSRKMSRHIEVARQKKLAKLAQPQCHVWRAKCDKCYNWDVGMWQLIETSLRHSNSLNC
>PILA_24501
MDWRELYVTPCSPALNVQHWSTKPVQFRETLSKYNEEMIKLAKTLQGLISESLGVEADAIQKRCGEGEQKVLMNYYPPCPRHDLTLGLKRHTDPGTLTILLQDKVGGLQATKGDGKTWVTVEPIDGAFIVNLKDQMHIDALEIEANMYAVGKIKHKYETMDKKKEKEEAISSNQPKYSHE
>PILA_26505
MNEYQVSCDEASGSIGLLPHTDGGSVTILHEDEVGGLQVLSKAGNWVEVKPRPNSFIVFLGDTLQGKGLDQNDEKTLLSEFRRCCEDWGCFRLTNHGIPAPLIERVEILCRDVFKLPPETKQKNVSPTLYGGYITGGSVAPYYESLGIGPDIEAIHEFSDLMWPQGNQNFW
>PILA_27976
MALQHAIAVTDVSTRAPHAFAAEDPSTAFTVFEVKPKSALVVSEAKGGRKADTVLPLGFEHRCPASIDSEGNLRVGFSEQEPTDLELSVHGKPCNLEQPVVKTEDAAKNLVGGHSAMNTKTYCLPERKAAEHYEDERPKVAYNQFSKDIPVISLSGIAGEERGRVRDEVSKACSEWGIFQVVDHGVPQELVDSMTQLSRAFFALPAEEKLKYDMRGGKRGGFVVSSHLQGEAVLDWREICIYFSYPLHQRDYTRWPVKPDGWRETVETYSESLMGLACKLLGIISEALGLEDQGLRGDGPESGDKLLSQMPRTRHDIGTEEAHRSCSPNEEKTYDNGEEKDSEVETEVDEENKEEDSSAEEKYDGSSHEGQTNSSEEEAKSIEEG
>PILA_29427
MARAVVELKTVKSLADMGIKDLPVNYVRDEDERPAVAYNDFSRDVPVISLANARAGGAEHARLIRELGAACEEWGMFQIVDHGVPTELNSQMMRCAEEFFSLPVEEKMQYAIQAGQFVGYGNGSFINDDPLMDWRELYVTACYPARDIHSWPAKPLQFREALANYSDTMMDLAKTLLGLISESLGVEADAIEKVCGEVQQKVLLNYYPTCPRPDLTLGLKRHTDPGTLTILLQDNVGGLQASKNGGETWVTVEPVDGAFVVNIGDHMHVLSNGMFKSADHQAVVNSNVRRQSIATFYNPDPESIVSPFDALAATKGEGQWIGLVIIYDVMRIIRQANIVVDDGSSTVVVGAWSCIDFGKAIEVFFSPLAVDVVLNGVDPTKPNIVKLGKPQSELRKLQLELSSLLLNASSSSSDILNSCQAGDFYFIFLHNMLPISAPEINPLLSLRARIMSTMKAE
>PILA_35198
MISSETNHILKTAHMPPNRRLPPNYVKEEDERPVVAYNDFSHEVPVISLAKARGGGAEREEVIREVRGACEEWGLFQITDHGVPTELNSHIMRCAEDFFSLPVEEKMQYAIEAGEFVGYGNGSFINDDPLMDWRELYVTSCSPALNVQHWSTKPVQFSEEMIKLAKTLLGLISESLGVEADAIQKRCGEGEQKVLMNYYPPCPRPDLTLGLKRHTDPGTLTILLQDKVGGLQATKDDGKTWVTVEPIDGAFVVNLGDQMHVLSNGIFKSTDHQAVVNSSALRQSIVTFYNPHPDSIVYPLDELVPGILPEEDDQTSPGQRDETQQGNYFTTATKRLIGQLSRLPEIQLSAVFTYGDATGESLGVEADAIQKRCGEGEQKVLMNYYPPCPRPDLTLGLNRHTDPGTLTILLQDKVGGLQATKDDGKTWVTVEPIDGAFVVNLGDQMHVLSNGIFKSADHQAVVNSSALRQSIVTFYNPHCLSA
>PILA_36171
MLELWRVLLELISEILGLEPEAIGKACGEGKQKVLLNYYPECPQPELALGLKRHTDPGVITIAVQDHGLQVTKDDGNNWVTVEPIEGAFVVFVGDILHILSNGRLKKGDHQAAVNSRHCTFKDLFKEKILRDYVRRAELKRKIEARSEKPADRVALLDNGILGQ
>PILA_36172
MLSRSHGDHSRALLSVHYQPFDNIMALSTVLSAAHQHADKDSVIEMARSGLKLLPACYVKSEDERPKITHNAFSKNVPVVSLLNSAGLERDRVKSELKQACGEWGVFQVVDHGVPQELNNRMMREAIGFFSLPVEEKYKYSFETGNFVGYASGSVYKDDPLMDWRELYAARGSPRDITTWPSQPPQMSQTLADYSDAMLELWRVLLELISEILGLESEAIQKACGEGEQKVLLNYYPECPQPELALGLKRHTDPGVITIALQDHVGGLQVTKDDGNNWVTVEPIEGAFVVFVGDILHFYFISQYSAERWKGTDPYYFFLFLDLK
>PILA_37865
MAPTAAVVNDRPDFLVTAEVPKSLQSIFIRDEDERPKVAYNQFSKDIPVISLYGVAGEERGRVRDEVSKACSEWGIFQVVDHGVPQELVDSMTQLSRAFFALPAEEKLKYDMRGGKRGGFVRDYTRWPVKPDGWREIVEKYSESLMGLACKLLGIISEALGLETEAEAEGEKCFMEEPITFAQMYSRKMSRDIELARQKKLAKVAEKEQSEISK

Gene tree

Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University