Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Phtheirospermum japonicum
Species name
Phtheirospermum japonicum is a species of eudicot in the family Orobanchaceae.
Species info
Gene sequence info
Phtheirospermum_japonicum.ANR.fa
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>Pjv1_00004032
MENGNNNTKVCVTGASGYIGSWLIKKLLEKGYIVHATLRNLGEESKTTLLKSLPMAETRLKLFQADIYNAQEFEAAIQGCQYVVHVATPLFHTQNSTYKDTTEAAIAGIRSIAESCLRSSTVKRLVYTSSVTAASPLREDSSGYKPQMDELCWTPLNLSSSYANDFLLVYMLMEILSYNNLCTGNGGKLEVVSLVCGLVGGETLLPYLSSSLPTVLAQLKGDQIAVNVLRFLQDLLGSIPLIHIEDVCEAHIFCIEKNILCGRFLCAADSCTTQDMANCYQQIHPQFQIAEGLKSGPKGRSKCELSKLRKEGYEFKYGLKDILNDSVECGKRMGYL
>Pjv1_00007538
MKEKTYCVTGATGYIGSWLVKSLLQRGYRVHATLRNPEKASHLFERWHGRDSRLSLFRADLQEDGSFDDAVRGCDGVFHVAASMELGVPAVQDLDSYIKTELTDPAIRGTLNVLKACLKSNITRVVFTSSISTLTAKDTSGGWLPIVDESCQTPIQQVLKSKASGWVYVLSKLLTEEAAFDFAEKNGIDLISVITTTVGGSFLTPTVPISIQVLLSPVTGDDKMLSILASVSSRMGSIALVHIEDICSAHIFLMEHPNAEGRYICCTQSCLMLESMERESIPSEISSKKIRDIGFEFKYSIQDIIHQTVRRCVDYGFLS
>Pjv1_00011155
MKTVCVTGASGFIASWLVKYLLQRGYTVKASVRDINDPKKTEHLVALDGAKERLKLIKADLLEEGSFDAVVDGCDGVFHTASPVFFATTDPQAELIDPALKGTLNVLRSCAKTPSIKRVVLTSSSGAVVFNGKPRTPEVVVDETWRSDPEFCRQAKQWYLLSKTLAEDAAWKFVREKGIDMVAINPGAETYPNTTLGWVNVKDIANAHILAFENPSANGRYLMVESVAHYSEIVKILRELYPTLPLPEKCADDKPFMPTYQVSKERAKSLGVEFTPLKEGIKETVESLKEKNLFPGEG
>Pjv1_00013535
MMKKKVCVTGGAGYLASFLIKHLLERGYIVHATLRSLGDASKVGLLKGLAYADTRLKLFEADIYNPCEFATAVEGCEIVVHMATPLQHYTNNSKYKNTSEAAVAGVKFIMENCVKSNTVTKLIYTASVVAVSPLKDDGSGYKDLMDETCWSPLNISYEFLSDYVHSKTLAEKEVLSYNGKGIEVVSLACGLVGGDTIQSFMSGSMGALISQATNDRAGYKTLRILEDLLAKVPIAHVQDVTEAHIFAMENIHINGRFIEDVNRETRWGSRKLENLGFKYKYDAEKIILDSVICAKRLGNII
>Pjv1_00017291
MCVTGTSGYIASWLVKLLLHRCYSVKATVRNLSDPKKVEHLLALEGAEERLRLFEANLVDEGSFDSVVDGCEAVFHTTSPIFMKVSDPQTELIDPAVNGMINVLRSCKKASSIKRVVVTSSMASVIFNSNPLTPGVVIDETWVSDPKVCEENKEYYALSKTLAEKAAWKFAEENGPDLVTLHPGYVIGPLLQPTLNFTSEATVSLIKEGIYIQIRIR
>Pjv1_00020970
MKVSDPQTELIDPAVNGTINVLRSCKKASSIKRVVVTSSMASVIFNSNPLTPGVVIDETWVSDPKVCEENKEYYALSKTLAEKAAWKFAEENGLDLVTLHPGYVIGPLLQPTLNFTSEAQFHL
>Pjv1_00019710
MIRCLSTKKEKMSGTVCVTGAGGYVASWLIKLLISHGYNVHGTLRNPGDEKYVHLKSLENASEKLKLFKADLLDFDSILAAVKGCDGVFHVASPVPSSSVEVVAPALEGTLNILKACSQAKIRRAVVVSSVAEAEALEYAKQNGLDVVTVCPTLVLGPMLQHAPNASSLFLIKLLREGFEEVENKLRSIVDVRDVADALKLVYEKREAEGRYICTAHRVKNQDLVQTLRQIYPSYNYPKSFKEGEELGPKLSSEKLQGLGWKYRPLTETLVDSVEMYKQAGLIHSS
MENGNNNTKVCVTGASGYIGSWLIKKLLEKGYIVHATLRNLGEESKTTLLKSLPMAETRLKLFQADIYNAQEFEAAIQGCQYVVHVATPLFHTQNSTYKDTTEAAIAGIRSIAESCLRSSTVKRLVYTSSVTAASPLREDSSGYKPQMDELCWTPLNLSSSYANDFLLVYMLMEILSYNNLCTGNGGKLEVVSLVCGLVGGETLLPYLSSSLPTVLAQLKGDQIAVNVLRFLQDLLGSIPLIHIEDVCEAHIFCIEKNILCGRFLCAADSCTTQDMANCYQQIHPQFQIAEGLKSGPKGRSKCELSKLRKEGYEFKYGLKDILNDSVECGKRMGYL
>Pjv1_00007538
MKEKTYCVTGATGYIGSWLVKSLLQRGYRVHATLRNPEKASHLFERWHGRDSRLSLFRADLQEDGSFDDAVRGCDGVFHVAASMELGVPAVQDLDSYIKTELTDPAIRGTLNVLKACLKSNITRVVFTSSISTLTAKDTSGGWLPIVDESCQTPIQQVLKSKASGWVYVLSKLLTEEAAFDFAEKNGIDLISVITTTVGGSFLTPTVPISIQVLLSPVTGDDKMLSILASVSSRMGSIALVHIEDICSAHIFLMEHPNAEGRYICCTQSCLMLESMERESIPSEISSKKIRDIGFEFKYSIQDIIHQTVRRCVDYGFLS
>Pjv1_00011155
MKTVCVTGASGFIASWLVKYLLQRGYTVKASVRDINDPKKTEHLVALDGAKERLKLIKADLLEEGSFDAVVDGCDGVFHTASPVFFATTDPQAELIDPALKGTLNVLRSCAKTPSIKRVVLTSSSGAVVFNGKPRTPEVVVDETWRSDPEFCRQAKQWYLLSKTLAEDAAWKFVREKGIDMVAINPGAETYPNTTLGWVNVKDIANAHILAFENPSANGRYLMVESVAHYSEIVKILRELYPTLPLPEKCADDKPFMPTYQVSKERAKSLGVEFTPLKEGIKETVESLKEKNLFPGEG
>Pjv1_00013535
MMKKKVCVTGGAGYLASFLIKHLLERGYIVHATLRSLGDASKVGLLKGLAYADTRLKLFEADIYNPCEFATAVEGCEIVVHMATPLQHYTNNSKYKNTSEAAVAGVKFIMENCVKSNTVTKLIYTASVVAVSPLKDDGSGYKDLMDETCWSPLNISYEFLSDYVHSKTLAEKEVLSYNGKGIEVVSLACGLVGGDTIQSFMSGSMGALISQATNDRAGYKTLRILEDLLAKVPIAHVQDVTEAHIFAMENIHINGRFIEDVNRETRWGSRKLENLGFKYKYDAEKIILDSVICAKRLGNII
>Pjv1_00017291
MCVTGTSGYIASWLVKLLLHRCYSVKATVRNLSDPKKVEHLLALEGAEERLRLFEANLVDEGSFDSVVDGCEAVFHTTSPIFMKVSDPQTELIDPAVNGMINVLRSCKKASSIKRVVVTSSMASVIFNSNPLTPGVVIDETWVSDPKVCEENKEYYALSKTLAEKAAWKFAEENGPDLVTLHPGYVIGPLLQPTLNFTSEATVSLIKEGIYIQIRIR
>Pjv1_00020970
MKVSDPQTELIDPAVNGTINVLRSCKKASSIKRVVVTSSMASVIFNSNPLTPGVVIDETWVSDPKVCEENKEYYALSKTLAEKAAWKFAEENGLDLVTLHPGYVIGPLLQPTLNFTSEAQFHL
>Pjv1_00019710
MIRCLSTKKEKMSGTVCVTGAGGYVASWLIKLLISHGYNVHGTLRNPGDEKYVHLKSLENASEKLKLFKADLLDFDSILAAVKGCDGVFHVASPVPSSSVEVVAPALEGTLNILKACSQAKIRRAVVVSSVAEAEALEYAKQNGLDVVTVCPTLVLGPMLQHAPNASSLFLIKLLREGFEEVENKLRSIVDVRDVADALKLVYEKREAEGRYICTAHRVKNQDLVQTLRQIYPSYNYPKSFKEGEELGPKLSSEKLQGLGWKYRPLTETLVDSVEMYKQAGLIHSS
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University