Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Phtheirospermum japonicum
Species name
Phtheirospermum japonicum is a species of eudicot in the family Orobanchaceae.
Species info
Gene sequence info
Phtheirospermum_japonicum.COMT.fa
Download
>Pjv1_00000213
MLDPLLIDPWHDASEWFRNDCASPFMAHHGTTLWERLGNDESGNRLFKEGMASDARCVASVLTKECRRVFEGLRSMVDVGGGTGMLAKGIASAFPELECIVFDLPHVVDGMEGGKNLRYVSGDMWECVPHSDAVLLKWTLHDWDDQESVKLLEKCKEAIIPSKNKGGKLIIIEMVVDDEKEEREATETKLFFDMLMMVEVTGKERTEKEWANLFSAAGFSTYNISLGLGLRSVIEVFP
>Pjv1_00011455
MGSTTKNHVASSPIDSSSEEEACLFAMQLASASVLPMVLKSAIELDLLELIKKAGPGAFVSPVELAAQIPTSNPEAHVMLDRILRLLASYNVLNCSLKTLPGGGAERLYGLAPVCRFLTKNEDGVSMAALLLMNQDKILMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGKDLRFNKVFNQGMSNHSTILMKKILDIYDGFEGLKTVVDVGGGTGATLDMIVSKHPSIKGINFDLPHVIDDAPSYPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYEALPENGKVILAECILPEVPDTGLATQNVVHVDVIMLAHNPGGKERTEKEFQALAKGAGFKEFHKVCCAYNSWIMELRK
>Pjv1_00009642
MQLDLFEIVARAGDGAHLSPSDIASQLPTRNNRTVVVLESLLRLLATHSLLTCSAFKLANGSIERRYGLAPAGRFFIGDENGASLAPYYAFVSKEGPVQLRHSYKDAVLEGGKIFERIHGKSYYGSMASDLESGKIFNDAMGAHSTILMKKVVKIYDGFKGLSSLVNVGGGNGSTLDIIISNDPSIRGINFDLPQVVQTAPSFKGIEHTGGDMFLEVPKGDAILLKFVLHNWGDDQCAKLLKNCYEALANKGKVIVMDYILPNTPQTDVHAKYASQMDMVMSALLEGKERTEDEFEAMAKEAGFAEFKVVCYVYGMWIMEFVKLV
>Pjv1_00013453
MQLATASVLPMVLKAAIELDLLELIKKAGPVSAADLAARIHTTNPAAPATLGRVLRLLAAHSVLNCRLESLPDGGVEPRYSLAPVCKFFTNNEDGVSTVPFLLMLQDKVMMEAWYHLKDAIVEGEVPFNKVHGMNAFEYMGTDPRFGTLFNQAMSNLSIMVMNKILETYNGFGDFNSVVDVGGGTGATLNMIISKYSSIKGINFDLPQVIEHAPPYPGVEHIAGNMFVSVPKADAIFLKWIFHDWSDEHCIKILKNCYESLPENGKLIWVDAVVPEAPDTGLPTKNVVNADLMMLAQSPSGRERTEKECLGLAKLAGFEKFKSVCYAYNTWVMEIGK
>Pjv1_00014128
MQLATASVLPMVLKSAIELDLLELIKKAGPGAFVSPAELAAQIPATNPAAQAMLGRTLRLLATHSVLNCRLESRPDGGNERLYSLAPVCKFLTKNEDGVSMAPLLLMIQDKVIMESWYHLKETILDGEIPFNKAHGLTLFEYMAKDQMFNKLFNEAMSNPSTMLMKRILETYDGFEGLKSMVDVGGGTGAILNMIISKYPSIKGINFDLPHVIQQAPSYPGVEHVGGDMFVSVPRADAIFMKWIYHDWTDELCLKLLKNCYEALAEHGKVIWVEFVLPDGPDNGLQTKNVVNFDMLMLSQTPGGEERTEDKIQALAKGAGFNEFSKICRAYDFWVMEIRK
>Pjv1_00014134
MAQADENEEKDFLLAMQLASGSVLPMTLKAAIELDLLELIKKAEPESTASASELAAFELAAQLPTTNPDAATMIDRILRLLAAYSVLTCSLKPLPGGGAERRYGLAPVCRFLTRNEDGVSLASCYSLLSQDKVFMETWYHLKDAVLEGGVPFDRAYGMNTFEYPAKDPRFNKVFNKGMSEASAIIIKKIVEKYKGFEGLKSLVDVGGGIGTSLKMILSKYPSIKGINFDLPHVIQDAPPYPDVEHVAGDMFVSVPKADAIFMKWICHDWTDEHCLKLLKNCYEAIPENAKVILLELILPEVPHNGLGAKNVLNFDLIMLTHNPGGRERTEREFQVLANVAGFKKFHKVCCAYNFWIMELMK
>Pjv1_00014130
MQLATATVLPMVLKSAIELNLLELIKKAGPAAYVSSADLAAQIPTTNPAARDILDRILRLLATHSVLNCRLDSLPDGGVERLYSLAPVCEFLTKNEDGVSVAPLLLLLQDKVFTESWYQLKDAIRDGEIPFKKAHGSTLFEYMAKDEMFNKLFNQAMSNSSTILMKEILETYDGFEGLKSMVDVGGGNGATLNTIISKYPSIKGINFDLSHVIRDAPSYPGVEHVVGDMFVSLPKADAIFMRWICHVWTDEQCLKLLKNCYEALPENGKVILAEYVLPDGPDNGVPTKNAVNVDLHVLSQTPGGKERTEKEFHALAKGAGFKEFHKVCCAYDYWVMEFCK
>Pjv1_00014132
MALKSAIELNLLELIKKAGPAAYVSPADLAAQIPTTNPAARDMLDRILRLLATHSVLNCRLERLPDGGVERLYSLAPVCEFLTKNEDGVSMAPLLLMFLDKVSMDSWYHLNDAFLKGEIPFNKAHGMSLFDYNGTDKRFNKLFNQAMSNSSTILMKKILETYDGFEGLKSMVDVGGGSGATLNMIISKYPSIKGINFDLPHVIEHAPSYSGVDHVGGDMFVSVPKANAIFMKWIIHDWSDEYCLKLLKNCYEALSENGKVICVEFVLTDGPDNGLPAKNMANFDLIVLGLTPGGKERTENEYQALAKGAGFKEFYKVCCSYDYWVMEFRK
>Pjv1_00014131
MQLATAFMLPMALKSAIELNLLELIKKAGPAAYVSPADLVAQIPTTNPAAQDMLDRILRLLVTHSVLNCRLQSLPDGGVERLYSLAPVCEFLTKNEDGASMAPLLLMLLDKVSMENWYHIKDAFLEGEMPFNKAHGMSLFDYNGIDKRFSKLFYEAMANPSTIKIKKILETYDGFEGLKSMVDIGGGSGSILNMIISKYPSIKGINFDLPHIIEHAPCYSGVDHVGGDMFVSVPKANAIFMMWILNYWSDEYCLKLLKNCYEALPENGKVICVEFVLSDGPDNGLPAKNMANFDLIVLGLSLGGKERTENEFQALAKGAGFKEFHKVCCAYDYWVMEFCK
>Pjv1_00015654
MEQVLIGSDDEAGVVALAACTSHIFPKALKAAIQLDLFEIIGGAHGDDLSPLEIASQLPTSNVDRTVVVLDSLLRLLVTHSLLTCSVRKLANGSIERRYGLAPAGRFFVGDENGASLAPYFAFVSREGPVQLSYKDAVLNGGKIFEKVHGKSYYGSMASDPESGKIFDNAMGAHSTMIMKKVVKIYDGFEGLSSLVNVGGGNGATLDIIASNYPSIRGINFDLPQVVQTAPSYKGIEHIGGDMFLQVPKGDAILLKYVLHNWGDEQCAKLLKNCYRALPNKGKLIVMDYILPNSPQTDVHAKYASQMDIVMAAMLEGKERTEDEFRAMAKDAGFAEFKVVCYVYGVWIMEFFKLV
>Pjv1_00018568
MSEHSTIIMNKILDKYDGFDGLKSLVDVGGGIGTSLSMIISKYPSIKCINFDLSQVIQDAPSYSK
>Pjv1_00018563
MVLRTAIELDLLELIKKLAGPAGGFVSPADIAAQLPAACTNPAAHIAVDRILRLLASYSILNCTLKDGVGRVYSLAPVCKYLTKNEDGVSMAPLCLLQYDKASIESWYSLKDVVLEYAVLKGGEGFAKTSFEFLGKNPSFNNLFNQAMSDHSTIIMNKILDKYDGFDGLKSLVDVGGGTGAALGMIASKYPSIKGINFDLAQVIQTAPTYPGVEHVAGDMFVSVPKGDAIFMKWVCHDWNDEECLKILKNCYKSLPRSGKVILAEYILPDLPDNGRRTQYPVQLDLILMAYSTVGGKERSENEFHALAKGAGFKEFKKVCCAHNCWILELHK
>Pjv1_00029376
MSPMLLMLQDKVIMEGWYPLKDAIVEGEIPFKKAFGMNIFEYMRIDQRFNKLSHQAMSNLSTMFMKKILESYNGFEGLKSVVDVGGGIGATLNTIISKYPSIKGINFDLSHVVEHAPPYPGVEHVAGDMFVSVPKADAIFLKWICHDWTDEQCLKLLKNCYEALPQNGKVILVEYVLPDGPDNGVPTQNTVNVDLLMLAQAPEGKERTEKEFMALANLACFKNFKSVCYAHNTWLMEIGK
MLDPLLIDPWHDASEWFRNDCASPFMAHHGTTLWERLGNDESGNRLFKEGMASDARCVASVLTKECRRVFEGLRSMVDVGGGTGMLAKGIASAFPELECIVFDLPHVVDGMEGGKNLRYVSGDMWECVPHSDAVLLKWTLHDWDDQESVKLLEKCKEAIIPSKNKGGKLIIIEMVVDDEKEEREATETKLFFDMLMMVEVTGKERTEKEWANLFSAAGFSTYNISLGLGLRSVIEVFP
>Pjv1_00011455
MGSTTKNHVASSPIDSSSEEEACLFAMQLASASVLPMVLKSAIELDLLELIKKAGPGAFVSPVELAAQIPTSNPEAHVMLDRILRLLASYNVLNCSLKTLPGGGAERLYGLAPVCRFLTKNEDGVSMAALLLMNQDKILMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGKDLRFNKVFNQGMSNHSTILMKKILDIYDGFEGLKTVVDVGGGTGATLDMIVSKHPSIKGINFDLPHVIDDAPSYPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYEALPENGKVILAECILPEVPDTGLATQNVVHVDVIMLAHNPGGKERTEKEFQALAKGAGFKEFHKVCCAYNSWIMELRK
>Pjv1_00009642
MQLDLFEIVARAGDGAHLSPSDIASQLPTRNNRTVVVLESLLRLLATHSLLTCSAFKLANGSIERRYGLAPAGRFFIGDENGASLAPYYAFVSKEGPVQLRHSYKDAVLEGGKIFERIHGKSYYGSMASDLESGKIFNDAMGAHSTILMKKVVKIYDGFKGLSSLVNVGGGNGSTLDIIISNDPSIRGINFDLPQVVQTAPSFKGIEHTGGDMFLEVPKGDAILLKFVLHNWGDDQCAKLLKNCYEALANKGKVIVMDYILPNTPQTDVHAKYASQMDMVMSALLEGKERTEDEFEAMAKEAGFAEFKVVCYVYGMWIMEFVKLV
>Pjv1_00013453
MQLATASVLPMVLKAAIELDLLELIKKAGPVSAADLAARIHTTNPAAPATLGRVLRLLAAHSVLNCRLESLPDGGVEPRYSLAPVCKFFTNNEDGVSTVPFLLMLQDKVMMEAWYHLKDAIVEGEVPFNKVHGMNAFEYMGTDPRFGTLFNQAMSNLSIMVMNKILETYNGFGDFNSVVDVGGGTGATLNMIISKYSSIKGINFDLPQVIEHAPPYPGVEHIAGNMFVSVPKADAIFLKWIFHDWSDEHCIKILKNCYESLPENGKLIWVDAVVPEAPDTGLPTKNVVNADLMMLAQSPSGRERTEKECLGLAKLAGFEKFKSVCYAYNTWVMEIGK
>Pjv1_00014128
MQLATASVLPMVLKSAIELDLLELIKKAGPGAFVSPAELAAQIPATNPAAQAMLGRTLRLLATHSVLNCRLESRPDGGNERLYSLAPVCKFLTKNEDGVSMAPLLLMIQDKVIMESWYHLKETILDGEIPFNKAHGLTLFEYMAKDQMFNKLFNEAMSNPSTMLMKRILETYDGFEGLKSMVDVGGGTGAILNMIISKYPSIKGINFDLPHVIQQAPSYPGVEHVGGDMFVSVPRADAIFMKWIYHDWTDELCLKLLKNCYEALAEHGKVIWVEFVLPDGPDNGLQTKNVVNFDMLMLSQTPGGEERTEDKIQALAKGAGFNEFSKICRAYDFWVMEIRK
>Pjv1_00014134
MAQADENEEKDFLLAMQLASGSVLPMTLKAAIELDLLELIKKAEPESTASASELAAFELAAQLPTTNPDAATMIDRILRLLAAYSVLTCSLKPLPGGGAERRYGLAPVCRFLTRNEDGVSLASCYSLLSQDKVFMETWYHLKDAVLEGGVPFDRAYGMNTFEYPAKDPRFNKVFNKGMSEASAIIIKKIVEKYKGFEGLKSLVDVGGGIGTSLKMILSKYPSIKGINFDLPHVIQDAPPYPDVEHVAGDMFVSVPKADAIFMKWICHDWTDEHCLKLLKNCYEAIPENAKVILLELILPEVPHNGLGAKNVLNFDLIMLTHNPGGRERTEREFQVLANVAGFKKFHKVCCAYNFWIMELMK
>Pjv1_00014130
MQLATATVLPMVLKSAIELNLLELIKKAGPAAYVSSADLAAQIPTTNPAARDILDRILRLLATHSVLNCRLDSLPDGGVERLYSLAPVCEFLTKNEDGVSVAPLLLLLQDKVFTESWYQLKDAIRDGEIPFKKAHGSTLFEYMAKDEMFNKLFNQAMSNSSTILMKEILETYDGFEGLKSMVDVGGGNGATLNTIISKYPSIKGINFDLSHVIRDAPSYPGVEHVVGDMFVSLPKADAIFMRWICHVWTDEQCLKLLKNCYEALPENGKVILAEYVLPDGPDNGVPTKNAVNVDLHVLSQTPGGKERTEKEFHALAKGAGFKEFHKVCCAYDYWVMEFCK
>Pjv1_00014132
MALKSAIELNLLELIKKAGPAAYVSPADLAAQIPTTNPAARDMLDRILRLLATHSVLNCRLERLPDGGVERLYSLAPVCEFLTKNEDGVSMAPLLLMFLDKVSMDSWYHLNDAFLKGEIPFNKAHGMSLFDYNGTDKRFNKLFNQAMSNSSTILMKKILETYDGFEGLKSMVDVGGGSGATLNMIISKYPSIKGINFDLPHVIEHAPSYSGVDHVGGDMFVSVPKANAIFMKWIIHDWSDEYCLKLLKNCYEALSENGKVICVEFVLTDGPDNGLPAKNMANFDLIVLGLTPGGKERTENEYQALAKGAGFKEFYKVCCSYDYWVMEFRK
>Pjv1_00014131
MQLATAFMLPMALKSAIELNLLELIKKAGPAAYVSPADLVAQIPTTNPAAQDMLDRILRLLVTHSVLNCRLQSLPDGGVERLYSLAPVCEFLTKNEDGASMAPLLLMLLDKVSMENWYHIKDAFLEGEMPFNKAHGMSLFDYNGIDKRFSKLFYEAMANPSTIKIKKILETYDGFEGLKSMVDIGGGSGSILNMIISKYPSIKGINFDLPHIIEHAPCYSGVDHVGGDMFVSVPKANAIFMMWILNYWSDEYCLKLLKNCYEALPENGKVICVEFVLSDGPDNGLPAKNMANFDLIVLGLSLGGKERTENEFQALAKGAGFKEFHKVCCAYDYWVMEFCK
>Pjv1_00015654
MEQVLIGSDDEAGVVALAACTSHIFPKALKAAIQLDLFEIIGGAHGDDLSPLEIASQLPTSNVDRTVVVLDSLLRLLVTHSLLTCSVRKLANGSIERRYGLAPAGRFFVGDENGASLAPYFAFVSREGPVQLSYKDAVLNGGKIFEKVHGKSYYGSMASDPESGKIFDNAMGAHSTMIMKKVVKIYDGFEGLSSLVNVGGGNGATLDIIASNYPSIRGINFDLPQVVQTAPSYKGIEHIGGDMFLQVPKGDAILLKYVLHNWGDEQCAKLLKNCYRALPNKGKLIVMDYILPNSPQTDVHAKYASQMDIVMAAMLEGKERTEDEFRAMAKDAGFAEFKVVCYVYGVWIMEFFKLV
>Pjv1_00018568
MSEHSTIIMNKILDKYDGFDGLKSLVDVGGGIGTSLSMIISKYPSIKCINFDLSQVIQDAPSYSK
>Pjv1_00018563
MVLRTAIELDLLELIKKLAGPAGGFVSPADIAAQLPAACTNPAAHIAVDRILRLLASYSILNCTLKDGVGRVYSLAPVCKYLTKNEDGVSMAPLCLLQYDKASIESWYSLKDVVLEYAVLKGGEGFAKTSFEFLGKNPSFNNLFNQAMSDHSTIIMNKILDKYDGFDGLKSLVDVGGGTGAALGMIASKYPSIKGINFDLAQVIQTAPTYPGVEHVAGDMFVSVPKGDAIFMKWVCHDWNDEECLKILKNCYKSLPRSGKVILAEYILPDLPDNGRRTQYPVQLDLILMAYSTVGGKERSENEFHALAKGAGFKEFKKVCCAHNCWILELHK
>Pjv1_00029376
MSPMLLMLQDKVIMEGWYPLKDAIVEGEIPFKKAFGMNIFEYMRIDQRFNKLSHQAMSNLSTMFMKKILESYNGFEGLKSVVDVGGGIGATLNTIISKYPSIKGINFDLSHVVEHAPPYPGVEHVAGDMFVSVPKADAIFLKWICHDWTDEQCLKLLKNCYEALPQNGKVILVEYVLPDGPDNGVPTQNTVNVDLLMLAQAPEGKERTEKEFMALANLACFKNFKSVCYAHNTWLMEIGK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University