Enzyme
A copper-containing glycoprotein that plays a key role in the yellow coloration of flowers such as Antirrhinum majus (snapdragon). The enzyme is a homologue of plant polyphenol oxidase [1] and catalyses two separate chemical transformations, i.e. 3-hydroxylation and oxidative cyclization (2',-dehydrogenation). H2O2 activates reaction (1) but inhibits reaction (2). Originally considered to act on the phenol but now thought to act mainly on the 4'-O-beta-D-glucoside in vivo [4].
AS1
Enzyme
Aureusidin synthase
Enzyme name
EC:1.21.3.6
Entry
Oxidoreductases;Catalysing the reaction X-H + Y-H = X-Y;With oxygen as acceptor
Class
2',4,4',6'-tetrahydroxychalcone 4'-O-beta-D-glucoside:oxygen oxidoreductase
Sysname
(1) 2',4,4',6'-tetrahydroxychalcone 4'-O-beta-D-glucoside + O2 = aureusidin 6-O-beta-D-glucoside + H2O [RN:R08008];(2) 2',3,4,4',6'-pentahydroxychalcone 4'-O-beta-D-glucoside + 1/2 O2 = aureusidin 6-O-beta-D-glucoside + H2O [RN:R08010];(3) 2',3,4,4',6'-pentahydroxychalcone 4'-O-beta-D-glucoside + O2 = bracteatin 6-O-beta-D-glucoside + H2O [RN:R08009]
Reaction
aureusidin 6-O-beta-D-glucoside [CPD:C16409];H2O [CPD:C00001];bracteatin 6-O-beta-D-glucoside [CPD:C16410]
Product
Species information
Pharus latifolius
Species name
Pharus latifolius is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Pharus_latifolius.AS1.fa
Download
>Phala.03G254100.1.v1.1
MASLSQLITKPTTFSLSSPWSFSPRTTALHSGAKPRRTIQCISCTSSTNHRGEQAAPDGPKLDRRNVLLGIGTLGATAGLLSPHRASADPVATPDISTCGAPDLPPAANVMTCCPPPSNAPPVDFAPPDASSPLRTRPATHSASPDYVAKFNRAMAAMKALPVDDPRSFAAQASIHCAYCDGSYSPHGFPGMELQVHNSWLFLPFHRCYLYFFERILGSLIGDPSFGIPFWNWDAPEGMRMPAMYFDQSSPLYDPLRDRRHAPPKLIDLDYSGREPRFSEKQQIDHNLSVMYRQMISLSPTPSLFFGSPYRAGDDPNQGPSPLENMPHGPVHIWCGDPNEPDGEDMGNFYSAGRDPLFYAHHANVDRMWSVWKGIDARRHTDLNDPDWLDASFLFYDESPKLVRIRVRDVLDTERLRYKYQDVSVPWTNARPTVTQKARSFFTPAARAADTNKAVKFPLTLDSTASITVKRPAMSRSKKEKKAKEEVLVIEGIEVDRDVSAKFDVFVNADDHRAVGPAGRELAGSFVNVPHRHKHDKMGRGIRTKLRLALNEQLEDLMAEGDESVVVTLVPRQGNGKVKVGGVKIELMD
>Phala.03G376900.1.v1.1
MDGNHRGSTPFSLCILLLCALFSTVITAVILSINPCFHPFTRTLLAASGIDPYLVSCADDDGSLATLESDKAAHGKPIVTDLRLCGKPKLPPHALPPYHCCPPMSATEPINFTFPDTNEPMRTRQPVQAVGAEYMAKYARAIALMKALPKSDPRSFYQQANVHCAYCTGTYRQQGHPNLHVQIHFSWLFFPFHRAYLYFFERIVAKLLDDPGYALPFWSWDVPEGMQMPPVFIDPASPLYDPIRNPMHVPPKVADLNFDIVEKNLTDDEQIQYNLRLMYKQMISNAALPSLFHGQPFRAGDAEMPGPGTVELAPHNTMHTWTGDITEPNVEDMGVYYSAGRDPIFYPHHANIDRLWDAWRRLGHRDYSDPDWLDASFLFYDEEARLVSITTRDVIDMDKLRYRYDGVGLPWVNARPPVTPGVNNKNRGLMSVAFPVTLDAAPVTAEVRRPRVPRSRQEKDMEEEVLVVDSIRTDGTEYVKFDVYVNAADYEKVPPGGREMAESFACLQNQGEESTETSMRVALNEILEDLGAEGDDSVTVTLVPRRGKATIGGLRIAYIVE
>Phala.03G254300.1.v1.1
MASSHSAPFSLCILLFCAAVAFTPIAALSLSINPCAYSLSRNLLAASGLDSYLISCAADTSTTLLSDGDHDDGNPGKPIVTDLRQCAKPDFPPHALPPFHCCPPTPASEPVNFTFPDPGEPLRTRRPVHAMSAEYMARYARAIALMKALPKSDPRSFYQQANIHCAYCTGAYRQVGHPELRVQIHFSWLFFPFHRAYIYFFERIVGKLLGDPEFAVPFWSWDVPEGMRIPAEFADRASPLYDSIRNPRHAPPKLVDLDFLDVEKNYTDEVQIQHNLRVMYKQMISNAPLPSLFHGQPYRAGDADKPGAGTIEIYPHNTMHGWTGDISRPNHEDMGVYYSAGRDPLFYPHHANIDRLWEAWRCIGRRHADFTDPDWLNASFLFYNEEARLVRVTIRDMLDMDKLRYTYEGVSLVWLNARPPVTPDVNSKKHALKSVKFPVSLDAAVTAEIRRPRVLRSRHEKEIEEEVLVVDGIETDGTDFVKFDVYVNAVEYEKVGPGGREMAGSFVCLKHRGNDGVEGKGIETSMRVALNELLEDLGVEGDETVTVTLVPKRGKVRIGALRIEYLVE
>Phala.03G254400.1.v1.1
MASHRPPLIILILAASLAFAASLSTNPCDYTPARTLLSLSGLDPFLISCGDGAVASAEAPRPHRNTTGPMITDLSACGPPDLPSHAMPPFHCCPPSSPDPPLDFTFPDPSEPLRVRRRMHTLSAELLANYTRAIELMKALPKSDPRSFYQQANIHCAYCAGAYRHLGFPELTIMVHNTWFFQPLHRGYLYFYERILGKLIGDPTFALPFWNWDAPDGMQMPAVFADPSSTFFDPIRNSNHTPPKYLNMDFDGHDPDYTDEQQIEHNLKIMYRQLITNAPLPSLFHGQPYRPGDKMNPGPGSLETYPHNAVHYWLGDLTRPNHEHMGVYYSAGRDPILYQHHSNIDRMWEVWRALDDRHVDFTDRDWLESSFLFYDENARLVRMRVRDTLETSKLRYTYEDVKLQWVNAKPAVTPNLNKKAPPLQAVRFPVALDSAVTVEVRRPRVSRSRLAKAVEEEVLVVEGIEFDGTEFVKFDVYINSPEYEKVGRSGRELAGSYVSLKSREKHDDTRGMKETETSFRLALNEVLEDLDAEEDETVTVTLVPRRGKVSVGGLRIEYLVE
>Phala.06G236300.1.v1.1
MEGTCIARSATPRMPCNVQNSRRNLSSHYKKHHKISCKATGGHVDRRDMLLGLGGAAAGLAMNRGALAAPIQAPDLANCGPPPDNLPESAPDDLNCCLPYSANTVITDFKLPSSSSPLRVRPAAHLVDQECVAKYERAVELMKNLPADDPRSFAQQWRVHCAFCDGAYDQVGFPNLELQVHNCWLFFPWHRFYLYFHERILGKLIGDDTFALPFWNWDAPDGMTLPAIYAKSSSSLYDERRNPVHQPPFPVDLDYSGTDPSIPRDQQINENLRIMYRQMISGAKKTVLFMGRPYRAGDQPDPGPGSIENVPHNPVHRWTGDPRQPNGEDMGTFYSAARDPIFFAHHGNVDRMWRLWRGLRPSNRDFTDPDWLDANFLFYDEEARLVRVRVRDCLDTVAMRYTYQDVPLPWLNAKPAVQARPGASKRAVTASTLPAKLDKAVTVTVTRPKVSRSRKEKDDEEEVLFVEGIEIANHNKYVKFDVYVNGSEGDGTAARQCAGSFALAPHMVRGGSVKTLGCFGIGELLEDIGADGDKTVTVTIVPRCAGDMVTVGSVRIGYTK
>Phala.08G170000.1.v1.1
MAGYSFFAPLSAISTSPACPFPKPSFTTTSLSKKPSHGHVSCKAGATGGGESDNDDRLFDRRDMLVGLTGAATATGGLTLSELALAADKKKCNGQAFDFARQGSVTCKVVPGKTVCTPELPTSEPIVRFQGPPRTEPIRARRPAHLVDSDYVAKYKKALGAMKALPKDDPHSFWYQAAIHEAFCSTNNYRYKRKVGEEGGFNVHFSWVFAPWHRMYIYFFERIVRKYAEDDSFTLPYWNWDTAEGMALPEMYKDRSSPLYNENRNQKHLDCFVDLTLTPTSEDRGICPKFDLNDKDTIDANLCLMYKQMVPNRTALQFLGDKYCTDDDSIMAAGKLEEMAHTAVHIWTGDQAKCDANNQNCGQDMGYLGTAGRDPVFYAHHANVDRMWHLWRTVLGRQDFQDREWLDTTFVFYDENRQLVSIKTSDVLDTSALKYKYEETGPLPWLDRKPQPAIAESDRAAARSAGKAGLLSSFGLDDTTFPINLPKKGEKVYLAVQRPAEAKGAAKQEEVLVLDVKLDPTRSAKFDVLINVPRGLEAKVGPRSSELAGSFVNLPHGPRGGGKTETMRLSVRFPLREPLTDIKCAEDDVVNVTIVQRSNEKVEVGSVRIELLE
>Phala.08G170100.1.v1.1
MASYSFFPPLSAMPASPACPFPTLKPTNSSTRRFSCKAAGDSGSNDDVRLDRRDVLLGLTGAAATGGGGGLTLAAPISVCRGAPFTEATLKCDRNKDKECLAPARIIDFSPPPASDPLRVRFPAHLADPSYVAKYEKAIQAMRDLDNTDPDDPRSFRHQAAVHDAFCNKMYKYKVGDKDIGFNVHFSWLFAPWHRMYIYFFERILGKLIGDKSFALPYWNWDTPAGMALPAMYRPVCSPLYDANREFTHLNCYVDLAYNFPEPDTCMPFDQNAKMNSMIEKNLCTVYNQMKRASKTTRSFLGEKYCSGDLDFDLQTGAGTLESMAHTAVHIWTGDLKFANDMGNLATAGRDPAFYVHHANVDRMWHIWRTELGNRDFRDPDWHNTQFIFYDENKDLVRVKISDSVDIAKLRYAYAPSDMPWLDKRPKPRKKLDKPSRSRVVAAATAAVPAALALEGPLPLPTDLKKEPVKVTLDRPAAALDEAKKEGKKEVLVVHGIEINPTKFAKFDVCINVPLAEAATVGPQDSEFAGSFVNVPHAGEEGVKLKTSLRLALDELLTDIGCAGDPSVNVVLVPRSKQAIKVKGVSIELQDASD
>Phala.12G163400.1.v1.1
MPSLSQLTTKSTTFSLSSPWPFSPRTTALHSGAKPRRTIQCISCTSSTNHRGEQAAPDGPKLDRRNVLLGIGTLGATAGLLSPHRASVDPVETPDISTCREPHLPPKMTCCPQPSNAPPVDFAPPDASSPLRTRPATHSASPDYVAKFNCAMAAMKALPADDPRSFAAQAGIHCAYCDGSYSPHGFPGMELQVHNSWLFLPFHRCYLYFFERILGSLIGDPSFGIPFWNRDAPEGMRMPAMYFDQSSPLYDPLRGRRHAPPKLIDLDYSGREPRFSEKQQIDHNLSVMYRQMISLSPTPSLFFGSPYRAGDDPNQGPSPLENMPHGTVHNWCGDPDELNCEDMGNFYSAGRDPLFYAHHANVDRMWSVWKGIDARRHTDLSDPDWLDASVLFYDESPKLVRIRVRDVLDTERLRYKYQDVSAPWTNARPTVTQKARSFFTPAARAADTNKAAKFPLTLDSTASITVKRPAMSRSKKEKKAKEEVLVIEGIEVDRDVSQDSVHFCCQQPHRARSSRPSLCAHSLVVNLVAFGAPNHVNLLHPSPSNAPNTTTSPKLHRNCRAAHAIAVTSVSRRTPANGPVVRAEACCHVPAMHLPATISTCAWRSWQAPAFTAMPDHRLGPVAAPPPGTATPSHASEISSRRPLAVFQSSLARLYKASTTSATMHLTITPPPCCHLLLR
MASLSQLITKPTTFSLSSPWSFSPRTTALHSGAKPRRTIQCISCTSSTNHRGEQAAPDGPKLDRRNVLLGIGTLGATAGLLSPHRASADPVATPDISTCGAPDLPPAANVMTCCPPPSNAPPVDFAPPDASSPLRTRPATHSASPDYVAKFNRAMAAMKALPVDDPRSFAAQASIHCAYCDGSYSPHGFPGMELQVHNSWLFLPFHRCYLYFFERILGSLIGDPSFGIPFWNWDAPEGMRMPAMYFDQSSPLYDPLRDRRHAPPKLIDLDYSGREPRFSEKQQIDHNLSVMYRQMISLSPTPSLFFGSPYRAGDDPNQGPSPLENMPHGPVHIWCGDPNEPDGEDMGNFYSAGRDPLFYAHHANVDRMWSVWKGIDARRHTDLNDPDWLDASFLFYDESPKLVRIRVRDVLDTERLRYKYQDVSVPWTNARPTVTQKARSFFTPAARAADTNKAVKFPLTLDSTASITVKRPAMSRSKKEKKAKEEVLVIEGIEVDRDVSAKFDVFVNADDHRAVGPAGRELAGSFVNVPHRHKHDKMGRGIRTKLRLALNEQLEDLMAEGDESVVVTLVPRQGNGKVKVGGVKIELMD
>Phala.03G376900.1.v1.1
MDGNHRGSTPFSLCILLLCALFSTVITAVILSINPCFHPFTRTLLAASGIDPYLVSCADDDGSLATLESDKAAHGKPIVTDLRLCGKPKLPPHALPPYHCCPPMSATEPINFTFPDTNEPMRTRQPVQAVGAEYMAKYARAIALMKALPKSDPRSFYQQANVHCAYCTGTYRQQGHPNLHVQIHFSWLFFPFHRAYLYFFERIVAKLLDDPGYALPFWSWDVPEGMQMPPVFIDPASPLYDPIRNPMHVPPKVADLNFDIVEKNLTDDEQIQYNLRLMYKQMISNAALPSLFHGQPFRAGDAEMPGPGTVELAPHNTMHTWTGDITEPNVEDMGVYYSAGRDPIFYPHHANIDRLWDAWRRLGHRDYSDPDWLDASFLFYDEEARLVSITTRDVIDMDKLRYRYDGVGLPWVNARPPVTPGVNNKNRGLMSVAFPVTLDAAPVTAEVRRPRVPRSRQEKDMEEEVLVVDSIRTDGTEYVKFDVYVNAADYEKVPPGGREMAESFACLQNQGEESTETSMRVALNEILEDLGAEGDDSVTVTLVPRRGKATIGGLRIAYIVE
>Phala.03G254300.1.v1.1
MASSHSAPFSLCILLFCAAVAFTPIAALSLSINPCAYSLSRNLLAASGLDSYLISCAADTSTTLLSDGDHDDGNPGKPIVTDLRQCAKPDFPPHALPPFHCCPPTPASEPVNFTFPDPGEPLRTRRPVHAMSAEYMARYARAIALMKALPKSDPRSFYQQANIHCAYCTGAYRQVGHPELRVQIHFSWLFFPFHRAYIYFFERIVGKLLGDPEFAVPFWSWDVPEGMRIPAEFADRASPLYDSIRNPRHAPPKLVDLDFLDVEKNYTDEVQIQHNLRVMYKQMISNAPLPSLFHGQPYRAGDADKPGAGTIEIYPHNTMHGWTGDISRPNHEDMGVYYSAGRDPLFYPHHANIDRLWEAWRCIGRRHADFTDPDWLNASFLFYNEEARLVRVTIRDMLDMDKLRYTYEGVSLVWLNARPPVTPDVNSKKHALKSVKFPVSLDAAVTAEIRRPRVLRSRHEKEIEEEVLVVDGIETDGTDFVKFDVYVNAVEYEKVGPGGREMAGSFVCLKHRGNDGVEGKGIETSMRVALNELLEDLGVEGDETVTVTLVPKRGKVRIGALRIEYLVE
>Phala.03G254400.1.v1.1
MASHRPPLIILILAASLAFAASLSTNPCDYTPARTLLSLSGLDPFLISCGDGAVASAEAPRPHRNTTGPMITDLSACGPPDLPSHAMPPFHCCPPSSPDPPLDFTFPDPSEPLRVRRRMHTLSAELLANYTRAIELMKALPKSDPRSFYQQANIHCAYCAGAYRHLGFPELTIMVHNTWFFQPLHRGYLYFYERILGKLIGDPTFALPFWNWDAPDGMQMPAVFADPSSTFFDPIRNSNHTPPKYLNMDFDGHDPDYTDEQQIEHNLKIMYRQLITNAPLPSLFHGQPYRPGDKMNPGPGSLETYPHNAVHYWLGDLTRPNHEHMGVYYSAGRDPILYQHHSNIDRMWEVWRALDDRHVDFTDRDWLESSFLFYDENARLVRMRVRDTLETSKLRYTYEDVKLQWVNAKPAVTPNLNKKAPPLQAVRFPVALDSAVTVEVRRPRVSRSRLAKAVEEEVLVVEGIEFDGTEFVKFDVYINSPEYEKVGRSGRELAGSYVSLKSREKHDDTRGMKETETSFRLALNEVLEDLDAEEDETVTVTLVPRRGKVSVGGLRIEYLVE
>Phala.06G236300.1.v1.1
MEGTCIARSATPRMPCNVQNSRRNLSSHYKKHHKISCKATGGHVDRRDMLLGLGGAAAGLAMNRGALAAPIQAPDLANCGPPPDNLPESAPDDLNCCLPYSANTVITDFKLPSSSSPLRVRPAAHLVDQECVAKYERAVELMKNLPADDPRSFAQQWRVHCAFCDGAYDQVGFPNLELQVHNCWLFFPWHRFYLYFHERILGKLIGDDTFALPFWNWDAPDGMTLPAIYAKSSSSLYDERRNPVHQPPFPVDLDYSGTDPSIPRDQQINENLRIMYRQMISGAKKTVLFMGRPYRAGDQPDPGPGSIENVPHNPVHRWTGDPRQPNGEDMGTFYSAARDPIFFAHHGNVDRMWRLWRGLRPSNRDFTDPDWLDANFLFYDEEARLVRVRVRDCLDTVAMRYTYQDVPLPWLNAKPAVQARPGASKRAVTASTLPAKLDKAVTVTVTRPKVSRSRKEKDDEEEVLFVEGIEIANHNKYVKFDVYVNGSEGDGTAARQCAGSFALAPHMVRGGSVKTLGCFGIGELLEDIGADGDKTVTVTIVPRCAGDMVTVGSVRIGYTK
>Phala.08G170000.1.v1.1
MAGYSFFAPLSAISTSPACPFPKPSFTTTSLSKKPSHGHVSCKAGATGGGESDNDDRLFDRRDMLVGLTGAATATGGLTLSELALAADKKKCNGQAFDFARQGSVTCKVVPGKTVCTPELPTSEPIVRFQGPPRTEPIRARRPAHLVDSDYVAKYKKALGAMKALPKDDPHSFWYQAAIHEAFCSTNNYRYKRKVGEEGGFNVHFSWVFAPWHRMYIYFFERIVRKYAEDDSFTLPYWNWDTAEGMALPEMYKDRSSPLYNENRNQKHLDCFVDLTLTPTSEDRGICPKFDLNDKDTIDANLCLMYKQMVPNRTALQFLGDKYCTDDDSIMAAGKLEEMAHTAVHIWTGDQAKCDANNQNCGQDMGYLGTAGRDPVFYAHHANVDRMWHLWRTVLGRQDFQDREWLDTTFVFYDENRQLVSIKTSDVLDTSALKYKYEETGPLPWLDRKPQPAIAESDRAAARSAGKAGLLSSFGLDDTTFPINLPKKGEKVYLAVQRPAEAKGAAKQEEVLVLDVKLDPTRSAKFDVLINVPRGLEAKVGPRSSELAGSFVNLPHGPRGGGKTETMRLSVRFPLREPLTDIKCAEDDVVNVTIVQRSNEKVEVGSVRIELLE
>Phala.08G170100.1.v1.1
MASYSFFPPLSAMPASPACPFPTLKPTNSSTRRFSCKAAGDSGSNDDVRLDRRDVLLGLTGAAATGGGGGLTLAAPISVCRGAPFTEATLKCDRNKDKECLAPARIIDFSPPPASDPLRVRFPAHLADPSYVAKYEKAIQAMRDLDNTDPDDPRSFRHQAAVHDAFCNKMYKYKVGDKDIGFNVHFSWLFAPWHRMYIYFFERILGKLIGDKSFALPYWNWDTPAGMALPAMYRPVCSPLYDANREFTHLNCYVDLAYNFPEPDTCMPFDQNAKMNSMIEKNLCTVYNQMKRASKTTRSFLGEKYCSGDLDFDLQTGAGTLESMAHTAVHIWTGDLKFANDMGNLATAGRDPAFYVHHANVDRMWHIWRTELGNRDFRDPDWHNTQFIFYDENKDLVRVKISDSVDIAKLRYAYAPSDMPWLDKRPKPRKKLDKPSRSRVVAAATAAVPAALALEGPLPLPTDLKKEPVKVTLDRPAAALDEAKKEGKKEVLVVHGIEINPTKFAKFDVCINVPLAEAATVGPQDSEFAGSFVNVPHAGEEGVKLKTSLRLALDELLTDIGCAGDPSVNVVLVPRSKQAIKVKGVSIELQDASD
>Phala.12G163400.1.v1.1
MPSLSQLTTKSTTFSLSSPWPFSPRTTALHSGAKPRRTIQCISCTSSTNHRGEQAAPDGPKLDRRNVLLGIGTLGATAGLLSPHRASVDPVETPDISTCREPHLPPKMTCCPQPSNAPPVDFAPPDASSPLRTRPATHSASPDYVAKFNCAMAAMKALPADDPRSFAAQAGIHCAYCDGSYSPHGFPGMELQVHNSWLFLPFHRCYLYFFERILGSLIGDPSFGIPFWNRDAPEGMRMPAMYFDQSSPLYDPLRGRRHAPPKLIDLDYSGREPRFSEKQQIDHNLSVMYRQMISLSPTPSLFFGSPYRAGDDPNQGPSPLENMPHGTVHNWCGDPDELNCEDMGNFYSAGRDPLFYAHHANVDRMWSVWKGIDARRHTDLSDPDWLDASVLFYDESPKLVRIRVRDVLDTERLRYKYQDVSAPWTNARPTVTQKARSFFTPAARAADTNKAAKFPLTLDSTASITVKRPAMSRSKKEKKAKEEVLVIEGIEVDRDVSQDSVHFCCQQPHRARSSRPSLCAHSLVVNLVAFGAPNHVNLLHPSPSNAPNTTTSPKLHRNCRAAHAIAVTSVSRRTPANGPVVRAEACCHVPAMHLPATISTCAWRSWQAPAFTAMPDHRLGPVAAPPPGTATPSHASEISSRRPLAVFQSSLARLYKASTTSATMHLTITPPPCCHLLLR
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University