Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Petunia inflata
Species name
Petunia integrifolia subsp. inflata is a subspecies of violet-flowered petunia (Petunia integrifolia)
Species info
Gene sequence info
Petunia_inflata.COMT.fa
Download
>Peinf101Scf00540g05026.1
MKCTVLDQPHVVGDCRGSGNLEFVGGSMFDKISDANAIFFKWVLHNWSDEDCVKILKKCKESIPSKEKGGKVIIVDTVLESPKLSNEFVQAQYYMDMVMMAFFAAKQRTKKEWEKIFTEAVIGSKIMSPTPFHTAHGKSFWEYIVEDQELGNLFNIMHWLVTRGLTSLVDVGGGTGTVAIAIAKAFPSLKCTVLDLPHVIGDLKGSRNLEFVGGDMFEKIPHANVILLKCILHDWGDEDCVKILKKCKESIPSREEGGKVIIIDTVMEDPKLRNDFVRAQHNMDMLMMVLCAAKERTTKEWEKLFTEAGFTLKLKPGTIGSTYFISSSAAKCAIELGVPDVLNKHGKPMSLSAALPLDPSKDSFLPILMRSLVNSNALFSKRKIIILLPQLVAFFEWFQNDSSSAFHTTYQKSPWDYFAEETILGDSFNDGMASDSTLIANVLITDCRHVFEGLTSLVDCSVLDLPHVVGNCRGSGNLEFVGGREMRVTVSDNHRHSAGKSKAER
>Peinf101Scf00614g03014.1
MGSSAKSQLPTQIEEERHCTYAMQLLTSSSALPFVLHSTVELEVFEILAKADGDTQLSPSQIVSHMPSCKNPDAATMLDRMLYLLASSSLLTCSIVEENGVPTRLYGLSPVGKFFVRDEDGASMGPLLALLQDKGFINSWFELKDAVLEGGVPFDRAHGVHAFEYTKLDPRFNDVFNKAMLNHTTVVMKKVLDNYKGFQDLEILVDVGGGLGVNLKMITSKYPSIKGKNFDLPHVVQHAPSYPGVEHVGGDMFESVPEGEAIFMKWILHDWSDNHCLKLLKNCYKALPDTGKVIVVESVLPVKPDTDSSVVSVAQYDLTMMAQSSGGKERSEHDFLALATEAGFKGIQLICCVCNFWVIEFYK
>Peinf101Scf00559g11010.1
MGSSANSQLPTQTDEERDCTYAMQLMSSSVLPLVLHSTVQLEVFEILAKAGADTQLSASQIVSHVPSCKNPDAATMLDRMLYVLASSSLLTCSIVDEKGVPTRLYGLSPVGKFFVRDEDGASMGPLLALMHDKVFINSWFELKDAVLEGVPFDRVYGVHAFEYPKSDPRFNDVFNKAMLNHTIIVMKKILDNYKGFEHLKTLVDVGGGLGINLKMITSKYPTIKGTNFDLLHVVEHASSYPGLEHVGGDMFESVPEGEAIFMKWILHDWSDSHCLKLLKNCYKALPDNGIVIVVEAILPVKPDTDSSVVGVTQFDLMMMVQNPGGKERSEHEFLALATEAGFKGIKLICCVCNFWVMEFYK
>Peinf101Scf00559g11014.1
FELKDAVLEGGVAFDRVHGVHVFEYTKLDPRFNDVFNKAMLNHTTAAMKKILDNYKGFEDLKILVDVGVILICRMLFNMPLPILWILHDWSDSHCLKLLKNCCKALPDNGKVIVVESVLPVKPDTDSSVVSVAQCDL
>Peinf101Scf00559g12016.1
MGSSANTQLPTQSEEERNCTYAMQLMSSSVLPFVLHSTVQLEVFEILANAGADSQLSASQIVCHMPSCKNPDAASMLDRMLYVLASSSLLTCSIVEENGVPTRLYGLSPVGKFFVRDEDGASMGPLLALLQDKVFVNSWFELKDAVLEGGVPFDRVHGVHAFEYPKSDPRFNDVFNKAMLNHTTIVMKKILDSYKGFEDLKALVDVGGGLGINLKMITSKYPTIKGTNFDLPHVVQHATSYPAVEHVGGDMFESVPEGEAIFMKWILHDWSDSHCLKLLKNCYKALPDNGKVIVVEAILPVKPDTDSSVLGVTQCDLTMMVQNPGGKERSEHEFLALATEAGFKGIKLICCVYNFWVMEFYK
>Peinf101Scf00765g05012.1
MEKGNGEASDNNNNARLAIMELANMISVPMSLNAVVKLKVADAIWEAGSNTALSATEILAKIRPSKDGDAENLQRILRMLTSYGVFDEHIVDDGSQRRYSLTEVGKTLVTDENGLSYGSYVLQHHQDALMKAWTIVHEAVNDSSTEPFVKANGEPAYDYYGKKPEMNSLMLNAMSGIAVPFMKAILESYDHGFQDVKTLVDVGGSGGDCLKMIIEKHSSIQQGINFDLPEVVAKAPQIPGIKHVGGDMFKYIPKGDAIFMKWVLTTWTDDECKQIMKNCYDALPKKGKFIACEPVLPHQTDESKRTRALLEGDIFVMTIYRAKGKHRTEEEYRQLGQSAGFAECRGFYTDHFFTILEFQKL
>Peinf101Scf00590g05003.1
MGNNFHDGMASDSRLTTDVLISECNKHVFEGLTSLVDVGGDLKGKSSHENLEFVGGSMFDEIPRANAILLKKSRYG
>Peinf101Scf01482g11032.1
MGSTSQNQTQTEEEAFLFAMQLASASVLPMVLKSAIELDLLEIMAKAGPGASISPSELAAQLPSQNTATPIMLDRMLRLLASYSVLDCTLRTLPDGKVERLYSLAPVCKFLTKNEDGVSVAPLLLMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTITMNKILKDYKGFEGLNSIVDVGGGTGATVNMIISKYPSIKGFNFDLPHVIEDAPSYPGVEHVGGDMFVSVPKADAIFMKWICHDWSDEHCLKFLNKCYEALPPNGKVIIAECILPEAPDTSLATKNVVHIDVIMLAHNPGGKERTEKDFEALAKGAGFKGFNKACCACNTWVMEFYK
>Peinf101Scf02460g00003.1
VEHVGGDMFESVPQGEVILVKWILHNWDDDNCLKILKNCWRALPEDGKVVVIEEIKEEYPVDNLISKHTFITDMIMMNFLAGGKERTKQEFEALAKEAGFATMKIVSRACCDRVIELYKR
>Peinf101Scf02460g00008.1
MDIVSSMDNIVISNEEENSLMRAMHIPSGFFLNMVLKVAIELGLFEIIAKSSELSSYEIAAHLPTKSPKAPLVLERILRFLASQSFLKCNITKDDGGHIHTSYNLTPLSKSLISSKDGTSVAPFLLLTTDPVFVSTWFHLKDAILEGEIPFNKAHGMHAFEYHEKDSRFNVVFNKAMQNLTCIETKRILESYVGFEGVKEVIDVGGGLGVLLASIVSKYPNIKGINFDLPHVIKDAPTYAGVEHIGGDMFENVPKGEVIILKAVLHDWDDDHCLKILKNCWRALPKDGKVVVIEQIQQENPESTNLLSKHALSTDILMATMLAGGKERTKQEFETLAKDAGFASIKIVCRAYYCWVLELLKY
>Peinf101Scf04170g00007.1
MDIVSSMDNIVISNEEENSLMRAMHIPSGFFLNMVLKVAIELGLFEIIAKSSELSSYEIAAHLPTKNPKAPLVLERFLRFLASQSFLKCNITKDDGGHIHTSYNLTPLSKSLISSKDGTSVAPFLLLTTDPVFVSSWFHLKDAILEGEIPFNKAHGMHAFEYHEKDSRFNVVFNKAMQNLTCIETKRILESYVGFEGVKEVIDVGGGLGVLLASIVSKYPNIKGINFDLPHVIKDAPTYAGVEHIGGDMFENVPKGEAILHDWDDGHCLKILKNCCRALPKDGKVVVIEQIQQENPESTNLLSKHALSTDILMATMLAGGKERTKQEFETLAKDAGFASIKIVCRAYYCWVMELLKY
>Peinf101Scf09895g00003.1
MAPSTDKIVITNEEENLLKALKIPTGLVLTMVLKAAIELGLFEIIEKSSQISSYEIASHLPTKNPKAPLILERILMFLASESLLTCNIIQDDNGCNKRLYSLTPLSIQGWRITCSVTLLLATDQTFDAIVDGETPFKKVHGMHMFEYLKKDSRFDKVFNKAMQHVTCLGMDKILEHYDGLQGVKQVVDVGGGLGVSLASIVYKYPHIKGVNFDLPHVIKDAPTYA
>Peinf101Scf09667g00010.1
MAPSTDKIVITNEEENLLKALKIPTGLVLTMVLKAAIELGLFEIIEKSSQISSYEIASHLPTKNPKAPLILERILMFLASESLLTCNIIKDDNGCNKRLYSLTPLSIHYLVSTKDGESLAPLLQLATDQIIDTILDGETPFKKVHGMHMFEYLKKDSVQQSYAACHLFGMDKILEHYDGLQGVKQVVDVGGGLGVSLASIVYKYPHIKGVEHVGGDMFERFPKGEVILVKWILHNSEDDNCLKILKNCWRALSEDGKVVVIEEIKQEYPADNLISKHAFTTDMIMMNLFAGGKERTKQEFEALAKEAGFATMNIVSRACCDWVIELYKR
MKCTVLDQPHVVGDCRGSGNLEFVGGSMFDKISDANAIFFKWVLHNWSDEDCVKILKKCKESIPSKEKGGKVIIVDTVLESPKLSNEFVQAQYYMDMVMMAFFAAKQRTKKEWEKIFTEAVIGSKIMSPTPFHTAHGKSFWEYIVEDQELGNLFNIMHWLVTRGLTSLVDVGGGTGTVAIAIAKAFPSLKCTVLDLPHVIGDLKGSRNLEFVGGDMFEKIPHANVILLKCILHDWGDEDCVKILKKCKESIPSREEGGKVIIIDTVMEDPKLRNDFVRAQHNMDMLMMVLCAAKERTTKEWEKLFTEAGFTLKLKPGTIGSTYFISSSAAKCAIELGVPDVLNKHGKPMSLSAALPLDPSKDSFLPILMRSLVNSNALFSKRKIIILLPQLVAFFEWFQNDSSSAFHTTYQKSPWDYFAEETILGDSFNDGMASDSTLIANVLITDCRHVFEGLTSLVDCSVLDLPHVVGNCRGSGNLEFVGGREMRVTVSDNHRHSAGKSKAER
>Peinf101Scf00614g03014.1
MGSSAKSQLPTQIEEERHCTYAMQLLTSSSALPFVLHSTVELEVFEILAKADGDTQLSPSQIVSHMPSCKNPDAATMLDRMLYLLASSSLLTCSIVEENGVPTRLYGLSPVGKFFVRDEDGASMGPLLALLQDKGFINSWFELKDAVLEGGVPFDRAHGVHAFEYTKLDPRFNDVFNKAMLNHTTVVMKKVLDNYKGFQDLEILVDVGGGLGVNLKMITSKYPSIKGKNFDLPHVVQHAPSYPGVEHVGGDMFESVPEGEAIFMKWILHDWSDNHCLKLLKNCYKALPDTGKVIVVESVLPVKPDTDSSVVSVAQYDLTMMAQSSGGKERSEHDFLALATEAGFKGIQLICCVCNFWVIEFYK
>Peinf101Scf00559g11010.1
MGSSANSQLPTQTDEERDCTYAMQLMSSSVLPLVLHSTVQLEVFEILAKAGADTQLSASQIVSHVPSCKNPDAATMLDRMLYVLASSSLLTCSIVDEKGVPTRLYGLSPVGKFFVRDEDGASMGPLLALMHDKVFINSWFELKDAVLEGVPFDRVYGVHAFEYPKSDPRFNDVFNKAMLNHTIIVMKKILDNYKGFEHLKTLVDVGGGLGINLKMITSKYPTIKGTNFDLLHVVEHASSYPGLEHVGGDMFESVPEGEAIFMKWILHDWSDSHCLKLLKNCYKALPDNGIVIVVEAILPVKPDTDSSVVGVTQFDLMMMVQNPGGKERSEHEFLALATEAGFKGIKLICCVCNFWVMEFYK
>Peinf101Scf00559g11014.1
FELKDAVLEGGVAFDRVHGVHVFEYTKLDPRFNDVFNKAMLNHTTAAMKKILDNYKGFEDLKILVDVGVILICRMLFNMPLPILWILHDWSDSHCLKLLKNCCKALPDNGKVIVVESVLPVKPDTDSSVVSVAQCDL
>Peinf101Scf00559g12016.1
MGSSANTQLPTQSEEERNCTYAMQLMSSSVLPFVLHSTVQLEVFEILANAGADSQLSASQIVCHMPSCKNPDAASMLDRMLYVLASSSLLTCSIVEENGVPTRLYGLSPVGKFFVRDEDGASMGPLLALLQDKVFVNSWFELKDAVLEGGVPFDRVHGVHAFEYPKSDPRFNDVFNKAMLNHTTIVMKKILDSYKGFEDLKALVDVGGGLGINLKMITSKYPTIKGTNFDLPHVVQHATSYPAVEHVGGDMFESVPEGEAIFMKWILHDWSDSHCLKLLKNCYKALPDNGKVIVVEAILPVKPDTDSSVLGVTQCDLTMMVQNPGGKERSEHEFLALATEAGFKGIKLICCVYNFWVMEFYK
>Peinf101Scf00765g05012.1
MEKGNGEASDNNNNARLAIMELANMISVPMSLNAVVKLKVADAIWEAGSNTALSATEILAKIRPSKDGDAENLQRILRMLTSYGVFDEHIVDDGSQRRYSLTEVGKTLVTDENGLSYGSYVLQHHQDALMKAWTIVHEAVNDSSTEPFVKANGEPAYDYYGKKPEMNSLMLNAMSGIAVPFMKAILESYDHGFQDVKTLVDVGGSGGDCLKMIIEKHSSIQQGINFDLPEVVAKAPQIPGIKHVGGDMFKYIPKGDAIFMKWVLTTWTDDECKQIMKNCYDALPKKGKFIACEPVLPHQTDESKRTRALLEGDIFVMTIYRAKGKHRTEEEYRQLGQSAGFAECRGFYTDHFFTILEFQKL
>Peinf101Scf00590g05003.1
MGNNFHDGMASDSRLTTDVLISECNKHVFEGLTSLVDVGGDLKGKSSHENLEFVGGSMFDEIPRANAILLKKSRYG
>Peinf101Scf01482g11032.1
MGSTSQNQTQTEEEAFLFAMQLASASVLPMVLKSAIELDLLEIMAKAGPGASISPSELAAQLPSQNTATPIMLDRMLRLLASYSVLDCTLRTLPDGKVERLYSLAPVCKFLTKNEDGVSVAPLLLMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTITMNKILKDYKGFEGLNSIVDVGGGTGATVNMIISKYPSIKGFNFDLPHVIEDAPSYPGVEHVGGDMFVSVPKADAIFMKWICHDWSDEHCLKFLNKCYEALPPNGKVIIAECILPEAPDTSLATKNVVHIDVIMLAHNPGGKERTEKDFEALAKGAGFKGFNKACCACNTWVMEFYK
>Peinf101Scf02460g00003.1
VEHVGGDMFESVPQGEVILVKWILHNWDDDNCLKILKNCWRALPEDGKVVVIEEIKEEYPVDNLISKHTFITDMIMMNFLAGGKERTKQEFEALAKEAGFATMKIVSRACCDRVIELYKR
>Peinf101Scf02460g00008.1
MDIVSSMDNIVISNEEENSLMRAMHIPSGFFLNMVLKVAIELGLFEIIAKSSELSSYEIAAHLPTKSPKAPLVLERILRFLASQSFLKCNITKDDGGHIHTSYNLTPLSKSLISSKDGTSVAPFLLLTTDPVFVSTWFHLKDAILEGEIPFNKAHGMHAFEYHEKDSRFNVVFNKAMQNLTCIETKRILESYVGFEGVKEVIDVGGGLGVLLASIVSKYPNIKGINFDLPHVIKDAPTYAGVEHIGGDMFENVPKGEVIILKAVLHDWDDDHCLKILKNCWRALPKDGKVVVIEQIQQENPESTNLLSKHALSTDILMATMLAGGKERTKQEFETLAKDAGFASIKIVCRAYYCWVLELLKY
>Peinf101Scf04170g00007.1
MDIVSSMDNIVISNEEENSLMRAMHIPSGFFLNMVLKVAIELGLFEIIAKSSELSSYEIAAHLPTKNPKAPLVLERFLRFLASQSFLKCNITKDDGGHIHTSYNLTPLSKSLISSKDGTSVAPFLLLTTDPVFVSSWFHLKDAILEGEIPFNKAHGMHAFEYHEKDSRFNVVFNKAMQNLTCIETKRILESYVGFEGVKEVIDVGGGLGVLLASIVSKYPNIKGINFDLPHVIKDAPTYAGVEHIGGDMFENVPKGEAILHDWDDGHCLKILKNCCRALPKDGKVVVIEQIQQENPESTNLLSKHALSTDILMATMLAGGKERTKQEFETLAKDAGFASIKIVCRAYYCWVMELLKY
>Peinf101Scf09895g00003.1
MAPSTDKIVITNEEENLLKALKIPTGLVLTMVLKAAIELGLFEIIEKSSQISSYEIASHLPTKNPKAPLILERILMFLASESLLTCNIIQDDNGCNKRLYSLTPLSIQGWRITCSVTLLLATDQTFDAIVDGETPFKKVHGMHMFEYLKKDSRFDKVFNKAMQHVTCLGMDKILEHYDGLQGVKQVVDVGGGLGVSLASIVYKYPHIKGVNFDLPHVIKDAPTYA
>Peinf101Scf09667g00010.1
MAPSTDKIVITNEEENLLKALKIPTGLVLTMVLKAAIELGLFEIIEKSSQISSYEIASHLPTKNPKAPLILERILMFLASESLLTCNIIKDDNGCNKRLYSLTPLSIHYLVSTKDGESLAPLLQLATDQIIDTILDGETPFKKVHGMHMFEYLKKDSVQQSYAACHLFGMDKILEHYDGLQGVKQVVDVGGGLGVSLASIVYKYPHIKGVEHVGGDMFERFPKGEVILVKWILHNSEDDNCLKILKNCWRALSEDGKVVVIEEIKQEYPADNLISKHAFTTDMIMMNLFAGGKERTKQEFEALAKEAGFATMNIVSRACCDWVIELYKR
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University