Enzyme
Isolated from the plants Perilla frutescens var. crispa, Verbena hybrida [1], Dahlia variabilis [2] and Gentiana triflora (clustered gentian) [3]. It will also act on anthocyanidin 3-O-(6-O-malonylglucoside) [2] and is much less active with hydroxycinnamoylglucose derivatives [3]. There is no activity in the absence of the 3-O-glucoside group.
PF3R4
Enzyme
Anthocyanidin 3-O-glucoside 5-O-glucosyltransferase 1
Enzyme name
EC:2.4.1.298
Entry
Transferases;Glycosyltransferases;Hexosyltransferases
Class
UDP-alpha-D-glucose:anthocyanidin-3-O-beta-D-glucoside 5-O-glucosyltransferase
Sysname
UDP-alpha-D-glucose + an anthocyanidin 3-O-beta-D-glucoside = UDP + an anthocyanidin 3,5-di-O-beta-D-glucoside [RN:R10262]
Reaction
UDP [CPD:C00015];anthocyanidin 3,5-di-O-beta-D-glucoside [CPD:C06361]
Product
Species information
Perilla citriodora
Species name
Perilla frutescens var. hirtella is a varietas of beefsteak-mint (Perilla frutescens)
Species info
Gene sequence info
Perilla_citriodora.PF3R4.fa
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>rna-gnl|WGS:QIYW|chr03_08908581_08909948.mrna1
MAETHILLVTFPAQGHINPSLQFAKRLSKHGVKITFLTSLYATSRMSQPSSFHGGAIDLLSFSDGSDAVWSVDETPKFMQALNDHGSKAVEDAVAARRAEGRPFTRVIYTLLVPWAGQVAHRVGVPSTLLWIQPAIVFGVYFHCYNKSFDAADEVIELPGLPVLRRSDLPSFFHDTNPSVYDFAIPTFTENFEMLDREDHPAVLLNTFDALELEPLRVLTNKYKLMPIGPLIPSAFLDGNDPTDKSFGADLIQKSEDYVQFLDSAEKETVIYVAFGSYSELSKPQMEAIEEGLIRSGRPFLWVIRGSENREKVDGILSRREELEKRGKIVAWCTQVEVLSHPSVGCFVTHCGWNSTLESLAAGVPLVALPQWTDQATNAKLVQDYWRSGVRAVKKEGNGEIVEADEIERCLEIVMGGGEMREQAKKWRDLAKEAIKEDGTSNVNLKIFVDQMINH
>rna-gnl|WGS:QIYW|chr03_08938988_08940427.mrna1
MLSNINVLIVTFPGQAHINPALQLAKNLAKRGVKVTFLTSLYALRRRRISSSSSSSSSSSSSSFHNLIDFVSFSDGYDDGWSVTPQFVSALRDHGSKAVEDAVAAKRREGRPFTRLIYTLSVPWAGAVAHRLDLPATLLWIQPAILFGVYFHYFNNNFDAIATDNEVIDLPGLPVLERRDLPSFLLPSTPSKFVLATFKENFENLDRDNHPTVLVNSFDALEAEALRVMSHYCRLMAVGPLIPSAYLDGGDDHDHDHDPNSDACFGGDLIPTSEDYEQYLDSKEEKSLVYVAFGSYTELSKPQMEEIAKGLITSGKPFLWVIRGSENKEKLDQLFSCREELEKQGKIVGWCNQVEVLSHPCVGCFVTHCGWNSTLESIASGVPMVLFPQWTDQATNAKLIQDFWRTGVRVVIPEKGGPVEGDEMKRCIEIVMAGGERGEQIRREAKKWRDLGKGAMKEDSGPSHANLHILINQLVAHQH
>rna-gnl|WGS:QIYW|chr03_08986579_08987961.mrna1
MVRRRVLLATFPAQGHINPALQFAKRLLKAGTDVTFFTSVYAWRRMANTASAAAGNPPGLDFVAFSDGYDDGLKPGGDGKRYMSEMKARGSEALRNLLLNNHDVTFVVYSHLFAWAAEVARESQVPSALLWVEPATVLCIYYFYFNGYADEIDAGSDEIQLPRLPPLEQRDLPTFLLPETPERFRLMMKEKLETLDGEEKAKVLVNTFDALEPDALTAIDRYELIGIGPLIPSAFLDGGDPSETSYGGDLFEKSEENNCVEWLDTKPKSSVVYVSFGSVLRFPKAQMEEIGKGLLACGRPFLWMIREQKNDDGEEEEEELSCIGELKKMGKIVSWCSQLEVLAHPALGCFVTHCGWNSAVESLSCGVPVVAVPQWFDQTTNAKLIEDAWGTGVRVRMNEGGGVDGSEIERCVEMVMDGGEKSKLVRENAIKWKTLAREAMGEDGSSLKNLNAFLHQVARA
>rna-gnl|WGS:QIYW|chr07_09360292_09361701.mrna1
MDRRHVLLVTFPVQGHINPTLQFAKRLINMGIDVTFATSISARRRMAKTATTNLAKGLTFVAFSDGYDDGYRLDQDDANNYMIQLRSLGTKALKETIRAASDDGRPITCLVYTLLLPWVPEVARDLHIPSALLWIQPATVLSIYYHYFNGFQDEITTNSNDDPSWNVQIPGAPLLSKRDLPSFLVPSSSDMYSFILPTLKEQLDALDAETKPRLLVNTFDALEPDALKAIDEYELIGIGPLIPSAFLDGKDPSDKSFGGDLFQKSDDYIEWLNSMPEASVVYVSFGSLLRLPKPQMEEIAKGLLDARRPFLWVIRANDNNEDDDDEDELSCLEDLVKIGKIVPWCSQLEVLTHPSLGCFVTHCGWNSTLESISCGVPVVAFPHWTDQGTNAKLIEDVWKTGVRVRFNEEDGIVESGEIWKCVEEVMDGGERSTQLKQNAHKWKDLARQATAENGSSTNNLKAFFDVIRP
>rna-gnl|WGS:QIYW|chr07_01712769_01714166.mrna1
MVNNNINVLIVTFPGQAHINPALQLAKNLAKRGVSITFLTSISSSSSSSSSSSSSSSSSFHNLIEFVSFSDGYDDGWSVTPEFISALRDHGSKAVEDAVAAKRGEGRPFTRLIYTLSVPWAGGVARRLGLPATLLWIQPAILFGVYFHYFNSNFDADDGGAIDLPGLPVLESRDLPSFLLPSTPSKFVLATFKENFENLDRDGGDRPTVLVNSFDALEAEALRGMSSYCRLIAVGPLLPSTAYLDDGGDDDDDLIPTPVEDYAEFLDSKEEKSVVYVAFGSYTELSKPQMEEIAKGLIASAKPFLWAIRGFESKEKMDQDLWEELEKQGKIVGWCNQVGVLSHPCVGCFVTHCGWNSTLESIASGVPMVLFPQWSDQATNAKLVQDLWGTGVRVVVPEKGGPVEGGEIRRCIEIVMGGGERGEEIRREAKKWRDLGKGAMKEDGGTSHANLNIFIHDQLYGGEER
MAETHILLVTFPAQGHINPSLQFAKRLSKHGVKITFLTSLYATSRMSQPSSFHGGAIDLLSFSDGSDAVWSVDETPKFMQALNDHGSKAVEDAVAARRAEGRPFTRVIYTLLVPWAGQVAHRVGVPSTLLWIQPAIVFGVYFHCYNKSFDAADEVIELPGLPVLRRSDLPSFFHDTNPSVYDFAIPTFTENFEMLDREDHPAVLLNTFDALELEPLRVLTNKYKLMPIGPLIPSAFLDGNDPTDKSFGADLIQKSEDYVQFLDSAEKETVIYVAFGSYSELSKPQMEAIEEGLIRSGRPFLWVIRGSENREKVDGILSRREELEKRGKIVAWCTQVEVLSHPSVGCFVTHCGWNSTLESLAAGVPLVALPQWTDQATNAKLVQDYWRSGVRAVKKEGNGEIVEADEIERCLEIVMGGGEMREQAKKWRDLAKEAIKEDGTSNVNLKIFVDQMINH
>rna-gnl|WGS:QIYW|chr03_08938988_08940427.mrna1
MLSNINVLIVTFPGQAHINPALQLAKNLAKRGVKVTFLTSLYALRRRRISSSSSSSSSSSSSSFHNLIDFVSFSDGYDDGWSVTPQFVSALRDHGSKAVEDAVAAKRREGRPFTRLIYTLSVPWAGAVAHRLDLPATLLWIQPAILFGVYFHYFNNNFDAIATDNEVIDLPGLPVLERRDLPSFLLPSTPSKFVLATFKENFENLDRDNHPTVLVNSFDALEAEALRVMSHYCRLMAVGPLIPSAYLDGGDDHDHDHDPNSDACFGGDLIPTSEDYEQYLDSKEEKSLVYVAFGSYTELSKPQMEEIAKGLITSGKPFLWVIRGSENKEKLDQLFSCREELEKQGKIVGWCNQVEVLSHPCVGCFVTHCGWNSTLESIASGVPMVLFPQWTDQATNAKLIQDFWRTGVRVVIPEKGGPVEGDEMKRCIEIVMAGGERGEQIRREAKKWRDLGKGAMKEDSGPSHANLHILINQLVAHQH
>rna-gnl|WGS:QIYW|chr03_08986579_08987961.mrna1
MVRRRVLLATFPAQGHINPALQFAKRLLKAGTDVTFFTSVYAWRRMANTASAAAGNPPGLDFVAFSDGYDDGLKPGGDGKRYMSEMKARGSEALRNLLLNNHDVTFVVYSHLFAWAAEVARESQVPSALLWVEPATVLCIYYFYFNGYADEIDAGSDEIQLPRLPPLEQRDLPTFLLPETPERFRLMMKEKLETLDGEEKAKVLVNTFDALEPDALTAIDRYELIGIGPLIPSAFLDGGDPSETSYGGDLFEKSEENNCVEWLDTKPKSSVVYVSFGSVLRFPKAQMEEIGKGLLACGRPFLWMIREQKNDDGEEEEEELSCIGELKKMGKIVSWCSQLEVLAHPALGCFVTHCGWNSAVESLSCGVPVVAVPQWFDQTTNAKLIEDAWGTGVRVRMNEGGGVDGSEIERCVEMVMDGGEKSKLVRENAIKWKTLAREAMGEDGSSLKNLNAFLHQVARA
>rna-gnl|WGS:QIYW|chr07_09360292_09361701.mrna1
MDRRHVLLVTFPVQGHINPTLQFAKRLINMGIDVTFATSISARRRMAKTATTNLAKGLTFVAFSDGYDDGYRLDQDDANNYMIQLRSLGTKALKETIRAASDDGRPITCLVYTLLLPWVPEVARDLHIPSALLWIQPATVLSIYYHYFNGFQDEITTNSNDDPSWNVQIPGAPLLSKRDLPSFLVPSSSDMYSFILPTLKEQLDALDAETKPRLLVNTFDALEPDALKAIDEYELIGIGPLIPSAFLDGKDPSDKSFGGDLFQKSDDYIEWLNSMPEASVVYVSFGSLLRLPKPQMEEIAKGLLDARRPFLWVIRANDNNEDDDDEDELSCLEDLVKIGKIVPWCSQLEVLTHPSLGCFVTHCGWNSTLESISCGVPVVAFPHWTDQGTNAKLIEDVWKTGVRVRFNEEDGIVESGEIWKCVEEVMDGGERSTQLKQNAHKWKDLARQATAENGSSTNNLKAFFDVIRP
>rna-gnl|WGS:QIYW|chr07_01712769_01714166.mrna1
MVNNNINVLIVTFPGQAHINPALQLAKNLAKRGVSITFLTSISSSSSSSSSSSSSSSSSFHNLIEFVSFSDGYDDGWSVTPEFISALRDHGSKAVEDAVAAKRGEGRPFTRLIYTLSVPWAGGVARRLGLPATLLWIQPAILFGVYFHYFNSNFDADDGGAIDLPGLPVLESRDLPSFLLPSTPSKFVLATFKENFENLDRDGGDRPTVLVNSFDALEAEALRGMSSYCRLIAVGPLLPSTAYLDDGGDDDDDLIPTPVEDYAEFLDSKEEKSVVYVAFGSYTELSKPQMEEIAKGLIASAKPFLWAIRGFESKEKMDQDLWEELEKQGKIVGWCNQVGVLSHPCVGCFVTHCGWNSTLESIASGVPMVLFPQWSDQATNAKLVQDLWGTGVRVVVPEKGGPVEGGEIRRCIEIVMGGGERGEEIRREAKKWRDLGKGAMKEDGGTSHANLNIFIHDQLYGGEER
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University