Enzyme
This plant enzyme, involved in the biosynthesis of anthocyanidins, is known to act on (+)-dihydrokaempferol, (+)-taxifolin, and (+)-dihydromyricetin, although some enzymes may act only on a subset of these compounds. Each dihydroflavonol is reduced to the corresponding cis-flavan-3,4-diol. NAD+ can act instead of NADP+, but more slowly.
DFR
Enzyme
Dihydroflavonol 4-reductase
Enzyme name
EC:1.1.1.219
Entry
Oxidoreductases;Acting on the CH-OH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3S,4S)-leucoanthocyanidin:NADP+ 4-oxidoreductase
Sysname
a (2R,3S,4S)-leucoanthocyanidin + NADP+ = a (2R,3R)-dihydroflavonol + NADPH + H+ [RN:R11500]
Reaction
(2R,3R)-dihydroflavonol [CPD:C21417];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Perilla citriodora
Species name
Perilla frutescens var. hirtella is a varietas of beefsteak-mint (Perilla frutescens)
Species info
Gene sequence info
Perilla_citriodora.DFR.fa
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>rna-gnl|WGS:QIYW|PC00007751_00147692_00149588.mrna1
MSLETVAAPPPATTVCVTGASGFIGSWIIMRLLERGYVVRATVRNPGDSKKVKHLIELPGADTNLTLWKADMNIEGSYDEAVQGCECVFHMATPMDFESDDPENEVIKPTVDGMLSIMRSCTKAKTVKKLIFTNSAGTLNVEEHQKPVYSEANWSDLDFIYSKKMTGWMYFVSKILAEKEAMKAAKESNINFISIIPPVVVGPFFMPTFPPSLITALSPITGNEAHYSIIKQGQFVHVDDLCEAHIFLFEHPEAEGRYICSSHDATIYDIAKMIGENWPEYHIPTEFEGIDKDIAVVSFSSKKLMDMGFSFKYTLEDMYRGAIESCREKGMLPFSTQIHTNGENKESLLNSQEKHSQINTNGEYKDSILNSQINTNGENKDPILNSHDKHSQIHTNGENEESILNSQEKHSQIRTNGENKESIFDSLEKHDTDNNQEKELLPIPKEKHADRQEMQI
>rna-gnl|WGS:QIYW|chr10_39009253_39011914.mrna1
MEGKNEMKGKTYCVTGATGYIGSWLVKSLLQRGCRVHATFRNPQKASHFLELWQGRDRLSLFRADLLEDGSFDEAVRGCDGVFHVSASMEFGVPASQNLDNCYVKTHITDPAVKGTLNVLKACLKTSTVRRIVFTSSISTLTAKDSSNRWVPLVDESCQTSVDRVLKTKASGWVYALSKLLTEEAAFRFAGENDIDLISVITTTVGGPFLTPTVPSSIQVLLSPVTGDPNMLPILASVNSRMGSIALVHIEDICNAHVFLMEHVNAEGRYLCCIQSCVVSELIELLKKEYPRSIALRLDGVEQDSVPSTISSKKIRDMGFEFKHDVEDILHQTVKKSVDYGFLPPLEKS
>rna-gnl|WGS:QIYW|chr03_09974717_09975936.mrna1
MEKSVKVCVTGGAGYLASFLIKHLLERGYTVHATLRSLSDASKVGLLKGLAHAETRLKLFEADIYNPVEFAAAIQGCSFVFHLATPLQHYANNPKYKNTSEAAVDGVKMIAESCMRSNTVKRLIYTASVMGASPCQDDASVYKDVVDESCWTPPNLSFEMFSAKSKTLAEKVVVGYNGKGIEVVSLACGLVGGETIQSFVSESMAVLISQAMNDGLRYRGLRVLEDLVSKVPIVHIQDVIEAHIFSMENSDISGRFLVATAFLKSVEIAILIHKHYPGITIPPE
>rna-gnl|WGS:QIYW|chr05_27250496_27267443.mrna1
MEKSVKVCVTGGAGYIASFLIKHLLERGYTVHATLRSLSDASKVGLLKGLSDAETRLKLFEGDIYNPAEFAPAIHGCDFVFHIATPLQHYQGSQYKNTSEAAVSGVKVIAESCIKSNTVKKLIYTASVTAASPLKDDGSGYKDVIDESCWTPLNLSFEMHSDYIKSKTCAEREVVKYNGRGIEVVSLGCGLVGGDTIQSLVSSSMAVLISQAMNATNLYRGLRSLEELMSKVPIAHIQDVIQAHIFSMENPHINGRFLVATAFLKSTEIASLIHKYHPDITIPPHMIEDTKRETKWGSRKLEDLGFHYKFDAHNIIHDSIVCAKRLGDI
MSLETVAAPPPATTVCVTGASGFIGSWIIMRLLERGYVVRATVRNPGDSKKVKHLIELPGADTNLTLWKADMNIEGSYDEAVQGCECVFHMATPMDFESDDPENEVIKPTVDGMLSIMRSCTKAKTVKKLIFTNSAGTLNVEEHQKPVYSEANWSDLDFIYSKKMTGWMYFVSKILAEKEAMKAAKESNINFISIIPPVVVGPFFMPTFPPSLITALSPITGNEAHYSIIKQGQFVHVDDLCEAHIFLFEHPEAEGRYICSSHDATIYDIAKMIGENWPEYHIPTEFEGIDKDIAVVSFSSKKLMDMGFSFKYTLEDMYRGAIESCREKGMLPFSTQIHTNGENKESLLNSQEKHSQINTNGEYKDSILNSQINTNGENKDPILNSHDKHSQIHTNGENEESILNSQEKHSQIRTNGENKESIFDSLEKHDTDNNQEKELLPIPKEKHADRQEMQI
>rna-gnl|WGS:QIYW|chr10_39009253_39011914.mrna1
MEGKNEMKGKTYCVTGATGYIGSWLVKSLLQRGCRVHATFRNPQKASHFLELWQGRDRLSLFRADLLEDGSFDEAVRGCDGVFHVSASMEFGVPASQNLDNCYVKTHITDPAVKGTLNVLKACLKTSTVRRIVFTSSISTLTAKDSSNRWVPLVDESCQTSVDRVLKTKASGWVYALSKLLTEEAAFRFAGENDIDLISVITTTVGGPFLTPTVPSSIQVLLSPVTGDPNMLPILASVNSRMGSIALVHIEDICNAHVFLMEHVNAEGRYLCCIQSCVVSELIELLKKEYPRSIALRLDGVEQDSVPSTISSKKIRDMGFEFKHDVEDILHQTVKKSVDYGFLPPLEKS
>rna-gnl|WGS:QIYW|chr03_09974717_09975936.mrna1
MEKSVKVCVTGGAGYLASFLIKHLLERGYTVHATLRSLSDASKVGLLKGLAHAETRLKLFEADIYNPVEFAAAIQGCSFVFHLATPLQHYANNPKYKNTSEAAVDGVKMIAESCMRSNTVKRLIYTASVMGASPCQDDASVYKDVVDESCWTPPNLSFEMFSAKSKTLAEKVVVGYNGKGIEVVSLACGLVGGETIQSFVSESMAVLISQAMNDGLRYRGLRVLEDLVSKVPIVHIQDVIEAHIFSMENSDISGRFLVATAFLKSVEIAILIHKHYPGITIPPE
>rna-gnl|WGS:QIYW|chr05_27250496_27267443.mrna1
MEKSVKVCVTGGAGYIASFLIKHLLERGYTVHATLRSLSDASKVGLLKGLSDAETRLKLFEGDIYNPAEFAPAIHGCDFVFHIATPLQHYQGSQYKNTSEAAVSGVKVIAESCIKSNTVKKLIYTASVTAASPLKDDGSGYKDVIDESCWTPLNLSFEMHSDYIKSKTCAEREVVKYNGRGIEVVSLGCGLVGGDTIQSLVSSSMAVLISQAMNATNLYRGLRSLEELMSKVPIAHIQDVIQAHIFSMENPHINGRFLVATAFLKSTEIASLIHKYHPDITIPPHMIEDTKRETKWGSRKLEDLGFHYKFDAHNIIHDSIVCAKRLGDI
Sheng jun & Dong yang Teams, Yunnan agriculture University