Enzyme
One of three specific glucosyltransferases in pea (Pisum sativum) that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside (see also EC 2.4.1.91, flavonol 3-O-glucosyltransferase and EC 2.4.1.240, flavonol-3-O-glycoside glucosyltransferase). TDP-glucose can replace UDP-glucose as the glucose donor but the reaction proceeds more slowly.
UGT79B30
Enzyme
UDP-glycosyltransferase 79B30
Enzyme name
EC:2.4.1.239
Entry
Transferases;Glycosyltransferases;Hexosyltransferases
Class
UDP-glucose:flavonol-3-O-beta-D-glucoside 2''-O-beta-D-glucosyltransferase
Sysname
UDP-glucose + a flavonol 3-O-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside [RN:R07339]
Reaction
UDP [CPD:C00015];flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside [CPD:C15581]
Product
Species information
Parasponia andersonii
Species name
Parasponia andersonii is a species of eudicot in the family Cannabaceae (hemp family).
Species info
Gene sequence info
Parasponia_andersonii.UGT79B30.fa
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>rna-gnl|WGS:JXTB|PanWU01x14_002300.1
MESSSSTMHIAMYPWFAFGHITPFLHLSNKLAQRGHRVSFLIPPKTESKIRHLNLLPDLITFYPITFPHVDGLPSGVETTNDVPVSSISHIAMAMDKTQADIQLLLRDLKPDFVLFDFAYWIPKLARPLGIISLHYSVVSPAATAYNASTSSHLHSLSGRQVSEADLMKPPPGYPDLPIYLHLHEARDYLMMTTLKLGSVTLFDRLFMSLSECEAIAVKGCREIDGPFVDYLKEDFGKPLLLTGPLIPDLQASPLEQKWVDFLGRFKPGSVIYCGFGSEVILTKGQFQELVLGLELSGFAFLAALRTPVGAESMDEALPDGFSERVGGRGVVHEGWIQQPQILKHPSVGCNVTHCGWGSLFEALMRKCQLVLIPNRGDQIYNARLMANTFKAGIEVEKGEEDGLFTRESVCKAIKTVMEEDTEVGKEVRANRAKLQELISHEEFDSSYIDGFIHELRTLLK
>rna-gnl|WGS:JXTB|PanWU01x14_022880.1
MSEQSKLHIAMYPWFAMGHLTSFLHISNKLAERGHRISFFIPTKTQSKLVSFNLHPDLIAFIPIQVPHVDGLPPGAETTADVPYPLLSFLMTAMDLTRPDIEAALLDLKPHFVFFDFTHWLPGSVRRFGIKAVHYCTVSPATVGFMLSPERTTITESALMEPPPSFPSSSIKLRVHEARDLAKVTLKEFGKGISFLERQQTSFNDSDAIAFKTSREMEGVYSDYVGEQWGKVVVLAGPVVPEPPKSVLEDKTASFLSRFGDKTVVYCAFGSECVLRKEQFQELVLGLELTGRPFLVALKPPVGFDTVESALPEGFEERVGRRGLVHGGWVQQQLILGHGSVGCFVTHCGSGSLLEAMVNECQLVLVPNVGDQIINARLMSGDLKVGVEVEKGNENGVYTTEGVCKAVEAVMEEESQVGQEVRANHKRWREFLLSKGLEDSYINGFIQKLHALRS
>rna-gnl|WGS:JXTB|PanWU01x14_026870.1
MESASSLHIVMTPWFAFGHITPYFHLSNKLAQKKHKISFVIPTKTQSKFNHLNQFPDLISFYPITVPHVDGLPYGAETTNDVPSSLIPLIMAAMDRTQNDIELLLRHLKPDLVFFDFLHWIPKLARGLGLKSLYYSTTTPVTMAYILSSDRNLITRRHLFSETDFMVPPPCYPDPLINLHLHEARALYEGIATKLNGDVLFFERWYMGLTQCDAVGFKACREIDGRFLDYLQKSLVKPFLVSGPLIPDPPVSSLEQKWVDFLGGFQPGSVVYCALGSEATLEKDQFQELLLGLELSGSPFLAALRLPFGVDSIHEALPLGFGERVKGRGVVHEGWIQQQKILEHPSIGCFVTHCGWGSLLEGLVSHCELVMLPHAGDQIFNARLMGKSLKVGVEVEKGEKDGLFSKESVCKAIRILMDDDSEVGKEIRINRAKLRDVLLRKDLESSYIDDFIHKLMTL
>rna-gnl|WGS:JXTB|PanWU01x14_289290.1
MEGSGSSFHIAMCPWFALGHINLYLHLSNKLAKKGHKSSFFIPTKTQSKIEHLNHFPDLITFYPITVPHVEGLPPGAETTNDVLPSLIPLIMTAMDRTENDLELLLRHLKPDFVFFDFVYWIPKLARRLGLKSLHYSVISPVTLAYNLSPSRHLITGRHQFSEMDFMQPPPGYPDSAINLHLHEARALLNESTAKFNGDVLFFDRWYVGFTQCDAIGFKACREIDGPFVDYLEKQFGKSLLLSGPLIPEPPISTLEQKWVRFLGGFKPASVVYCAFGSEATLTQDQLQELVLGLDLSGLPFLAALRPPSGVESIDEALPEGFVERVKGRGVVHGGWIQQPQILEHPSIGCHVTHCGVGSLFEALMNRCQLVFIPFLSDQIFNARLMANSLKVGSEVEKGEENGLFSRDGVCKAIKIVMEEDNEVGREIRANRAKLRELILHKDFESSYIDDFLGKLRTLLDRS
>rna-gnl|WGS:JXTB|PanWU01x14_289390.1
MGAPSSLHIVMYPRSAFGHIIPFLQLSNKLAEKGHRISFFIPTNTQSKIDHLNQFPDLISFVPIAVPHVDGLPSGSETTNDVPSTLQPLITIAMDRTEPQVELLLRYLKPDFVFFDFAHWIPKLARRLGIKSLSYVTSGPVFVSYVLSPARERQLDVGRQLSEADVMQPPPGFPDSSFKIHFHEARAFLRFKNMKFGSNIGFRHRLVTSLSECDALGFKSCREIDGPFLDYLETQFRKPLLLSGPLFPNPSSTLDEKWVNWLGGFKPGSVVYCALGSEATLREDQFKELLLGLELSGLPFLAALRPPSGVKPSSVVKALPDGFQERVGGRGVVHEGWIQQPQILEHPSVGCYVTHCGWGSLMEGVVNQCELVMIPRVGDQYYNARLMGQSLKVGIEVEKDEEDGLFTKESVCKAIRTVIEEDNEFGREVRANHAKLRKLLLRKDMESSYIDDFSQKLQSLLLS
>rna-gnl|WGS:JXTB|PanWU01x14_289370.1
MAESPSSLHIAMYPWFAFGHFTPFLQLSNKLAQKGHTISFFIPTKSQTKVEHLNHFPDLITFVPITLPYVDGLPSGAETTHDVHRSLFPLIMTAMDRTEPQVELLLRDLKPDFVFFDFLHWIPNLARRLGVKSLAYITGGSEFVSYVLSPERERQLDGLGRQLSEADVMQPPPGFPDLSFKIHLHEARGFLTFWSMKFGSNILFRHRMVTGLSECDALGFKACGEIDGPFLNYLEMQFGKPLLLSGPLFPKPSTSLDEKWVNWLGRFKPGSVVYCAFGSEATLREDQFKELVLGLKLSGHPFLAALRPPSGVDSIEKALPDGFRARVGGRGVVHECWIQQPQILEHPSVGCFVTHCGSGSLMEGLVNQCELVMIPQVLDQYYNARLIGNSLKVGIEVEKDEEEGLFTKESVCKAIRTVMEEDNEIGREIRANHTKLRNLLLRKDFESSYIDDFSQKLKNLVSLG
>rna-gnl|WGS:JXTB|PanWU01x14_289330.1
MEAPNSSLHIAMYPWFAFGHITPFLYLSNKLAQKGHKISFFIPTKTQAKVDHFNRFPDLLTFVPITVPHVDGLPSGAETTHDVPRSLFPLLMTAMDRTEPDVELLLRDLKPDFVFFDFVHWIPELARRLGIKSLVYGTGSPMIVAYNFSPARETQLNGVGRQLSEADIMQPPPGFPDSSIKLHLHEAQAWLMFRNLKFGSDILFRDRLLTGLTECEALGFKACREMEGPFIDYLEAQLGKPLLLSGPLLPDHTSSTTLGEKWVNWLGGFEPGSTVYCALGSEVILSKDQFQELLLGLELSGLHFLAALRPPSGAESSSIEDALPDGFLERVGGRGVVHGGWVQQPQILEHPSVGCFVTHCGWGSLMEALVNTCELVLIPNRGDQFYNARLLGSSLKVGIEVEKGEEDGLFTRESVSKAIRMVMEEDSEVGREVRANRSKLRELLIRKDMESSYIDDFILKLQSLLG
>rna-gnl|WGS:JXTB|PanWU01x14_289380.1
VILTRILDIYCHSLNPEELVNNNMFTDFFTGNSLSMAKAPSSLHIAMYPWFALGHFTPFLLLSNKLAQKGHTISFFIPTKSQTKVEHLNHFPDLITFVPINVPHVDGLPSGAETTHDVHRSLFPLIMTAMDRTEPEVELLLRHLKPHFIFFDFLHWIPNLARRLGIKSLAYITGGAVFVSYVLSPARERQLDVGRELSEADFMRPPLGCPDSSSFEIHLHEARGFLRFWSMKFGSDILFRHRLITCLSECDALGFRACREIDGPFLDYLETQFRKPLLISGPLLPNPGTTLDEKWVNWLGGFNRASVVYCAFGSEATLREDQFKELVLGLELSGLPFLAALRPPSGVESIEKALPDGFRERVGGRGVVHEGWVQQPQILEHPSVGCFVTHCGCGSLTEGLVNQCELVMIPHFLDQSYNARLIGNSLKVGIEAKKDEEDGLFSKESVCKAIRTVMEEDNEFGREVRANHAKLRKLLRRTEIESSYIDDFSQKLQNLILPG
MESSSSTMHIAMYPWFAFGHITPFLHLSNKLAQRGHRVSFLIPPKTESKIRHLNLLPDLITFYPITFPHVDGLPSGVETTNDVPVSSISHIAMAMDKTQADIQLLLRDLKPDFVLFDFAYWIPKLARPLGIISLHYSVVSPAATAYNASTSSHLHSLSGRQVSEADLMKPPPGYPDLPIYLHLHEARDYLMMTTLKLGSVTLFDRLFMSLSECEAIAVKGCREIDGPFVDYLKEDFGKPLLLTGPLIPDLQASPLEQKWVDFLGRFKPGSVIYCGFGSEVILTKGQFQELVLGLELSGFAFLAALRTPVGAESMDEALPDGFSERVGGRGVVHEGWIQQPQILKHPSVGCNVTHCGWGSLFEALMRKCQLVLIPNRGDQIYNARLMANTFKAGIEVEKGEEDGLFTRESVCKAIKTVMEEDTEVGKEVRANRAKLQELISHEEFDSSYIDGFIHELRTLLK
>rna-gnl|WGS:JXTB|PanWU01x14_022880.1
MSEQSKLHIAMYPWFAMGHLTSFLHISNKLAERGHRISFFIPTKTQSKLVSFNLHPDLIAFIPIQVPHVDGLPPGAETTADVPYPLLSFLMTAMDLTRPDIEAALLDLKPHFVFFDFTHWLPGSVRRFGIKAVHYCTVSPATVGFMLSPERTTITESALMEPPPSFPSSSIKLRVHEARDLAKVTLKEFGKGISFLERQQTSFNDSDAIAFKTSREMEGVYSDYVGEQWGKVVVLAGPVVPEPPKSVLEDKTASFLSRFGDKTVVYCAFGSECVLRKEQFQELVLGLELTGRPFLVALKPPVGFDTVESALPEGFEERVGRRGLVHGGWVQQQLILGHGSVGCFVTHCGSGSLLEAMVNECQLVLVPNVGDQIINARLMSGDLKVGVEVEKGNENGVYTTEGVCKAVEAVMEEESQVGQEVRANHKRWREFLLSKGLEDSYINGFIQKLHALRS
>rna-gnl|WGS:JXTB|PanWU01x14_026870.1
MESASSLHIVMTPWFAFGHITPYFHLSNKLAQKKHKISFVIPTKTQSKFNHLNQFPDLISFYPITVPHVDGLPYGAETTNDVPSSLIPLIMAAMDRTQNDIELLLRHLKPDLVFFDFLHWIPKLARGLGLKSLYYSTTTPVTMAYILSSDRNLITRRHLFSETDFMVPPPCYPDPLINLHLHEARALYEGIATKLNGDVLFFERWYMGLTQCDAVGFKACREIDGRFLDYLQKSLVKPFLVSGPLIPDPPVSSLEQKWVDFLGGFQPGSVVYCALGSEATLEKDQFQELLLGLELSGSPFLAALRLPFGVDSIHEALPLGFGERVKGRGVVHEGWIQQQKILEHPSIGCFVTHCGWGSLLEGLVSHCELVMLPHAGDQIFNARLMGKSLKVGVEVEKGEKDGLFSKESVCKAIRILMDDDSEVGKEIRINRAKLRDVLLRKDLESSYIDDFIHKLMTL
>rna-gnl|WGS:JXTB|PanWU01x14_289290.1
MEGSGSSFHIAMCPWFALGHINLYLHLSNKLAKKGHKSSFFIPTKTQSKIEHLNHFPDLITFYPITVPHVEGLPPGAETTNDVLPSLIPLIMTAMDRTENDLELLLRHLKPDFVFFDFVYWIPKLARRLGLKSLHYSVISPVTLAYNLSPSRHLITGRHQFSEMDFMQPPPGYPDSAINLHLHEARALLNESTAKFNGDVLFFDRWYVGFTQCDAIGFKACREIDGPFVDYLEKQFGKSLLLSGPLIPEPPISTLEQKWVRFLGGFKPASVVYCAFGSEATLTQDQLQELVLGLDLSGLPFLAALRPPSGVESIDEALPEGFVERVKGRGVVHGGWIQQPQILEHPSIGCHVTHCGVGSLFEALMNRCQLVFIPFLSDQIFNARLMANSLKVGSEVEKGEENGLFSRDGVCKAIKIVMEEDNEVGREIRANRAKLRELILHKDFESSYIDDFLGKLRTLLDRS
>rna-gnl|WGS:JXTB|PanWU01x14_289390.1
MGAPSSLHIVMYPRSAFGHIIPFLQLSNKLAEKGHRISFFIPTNTQSKIDHLNQFPDLISFVPIAVPHVDGLPSGSETTNDVPSTLQPLITIAMDRTEPQVELLLRYLKPDFVFFDFAHWIPKLARRLGIKSLSYVTSGPVFVSYVLSPARERQLDVGRQLSEADVMQPPPGFPDSSFKIHFHEARAFLRFKNMKFGSNIGFRHRLVTSLSECDALGFKSCREIDGPFLDYLETQFRKPLLLSGPLFPNPSSTLDEKWVNWLGGFKPGSVVYCALGSEATLREDQFKELLLGLELSGLPFLAALRPPSGVKPSSVVKALPDGFQERVGGRGVVHEGWIQQPQILEHPSVGCYVTHCGWGSLMEGVVNQCELVMIPRVGDQYYNARLMGQSLKVGIEVEKDEEDGLFTKESVCKAIRTVIEEDNEFGREVRANHAKLRKLLLRKDMESSYIDDFSQKLQSLLLS
>rna-gnl|WGS:JXTB|PanWU01x14_289370.1
MAESPSSLHIAMYPWFAFGHFTPFLQLSNKLAQKGHTISFFIPTKSQTKVEHLNHFPDLITFVPITLPYVDGLPSGAETTHDVHRSLFPLIMTAMDRTEPQVELLLRDLKPDFVFFDFLHWIPNLARRLGVKSLAYITGGSEFVSYVLSPERERQLDGLGRQLSEADVMQPPPGFPDLSFKIHLHEARGFLTFWSMKFGSNILFRHRMVTGLSECDALGFKACGEIDGPFLNYLEMQFGKPLLLSGPLFPKPSTSLDEKWVNWLGRFKPGSVVYCAFGSEATLREDQFKELVLGLKLSGHPFLAALRPPSGVDSIEKALPDGFRARVGGRGVVHECWIQQPQILEHPSVGCFVTHCGSGSLMEGLVNQCELVMIPQVLDQYYNARLIGNSLKVGIEVEKDEEEGLFTKESVCKAIRTVMEEDNEIGREIRANHTKLRNLLLRKDFESSYIDDFSQKLKNLVSLG
>rna-gnl|WGS:JXTB|PanWU01x14_289330.1
MEAPNSSLHIAMYPWFAFGHITPFLYLSNKLAQKGHKISFFIPTKTQAKVDHFNRFPDLLTFVPITVPHVDGLPSGAETTHDVPRSLFPLLMTAMDRTEPDVELLLRDLKPDFVFFDFVHWIPELARRLGIKSLVYGTGSPMIVAYNFSPARETQLNGVGRQLSEADIMQPPPGFPDSSIKLHLHEAQAWLMFRNLKFGSDILFRDRLLTGLTECEALGFKACREMEGPFIDYLEAQLGKPLLLSGPLLPDHTSSTTLGEKWVNWLGGFEPGSTVYCALGSEVILSKDQFQELLLGLELSGLHFLAALRPPSGAESSSIEDALPDGFLERVGGRGVVHGGWVQQPQILEHPSVGCFVTHCGWGSLMEALVNTCELVLIPNRGDQFYNARLLGSSLKVGIEVEKGEEDGLFTRESVSKAIRMVMEEDSEVGREVRANRSKLRELLIRKDMESSYIDDFILKLQSLLG
>rna-gnl|WGS:JXTB|PanWU01x14_289380.1
VILTRILDIYCHSLNPEELVNNNMFTDFFTGNSLSMAKAPSSLHIAMYPWFALGHFTPFLLLSNKLAQKGHTISFFIPTKSQTKVEHLNHFPDLITFVPINVPHVDGLPSGAETTHDVHRSLFPLIMTAMDRTEPEVELLLRHLKPHFIFFDFLHWIPNLARRLGIKSLAYITGGAVFVSYVLSPARERQLDVGRELSEADFMRPPLGCPDSSSFEIHLHEARGFLRFWSMKFGSDILFRHRLITCLSECDALGFRACREIDGPFLDYLETQFRKPLLISGPLLPNPGTTLDEKWVNWLGGFNRASVVYCAFGSEATLREDQFKELVLGLELSGLPFLAALRPPSGVESIEKALPDGFRERVGGRGVVHEGWVQQPQILEHPSVGCFVTHCGCGSLTEGLVNQCELVMIPHFLDQSYNARLIGNSLKVGIEAKKDEEDGLFSKESVCKAIRTVMEEDNEFGREVRANHAKLRKLLRRTEIESSYIDDFSQKLQNLILPG
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University