Enzyme
This enzyme is a member of the aromatic amino acid lyase family, other members of which are EC 4.3.1.3 (histidine ammonia-lyase) and EC 4.3.1.23 (tyrosine ammonia-lyase) and EC 4.3.1.25 (phenylalanine/tyrosine ammonia-lyase). The enzyme contains the cofactor 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO), which is common to this family [3]. This unique cofactor is formed autocatalytically by cyclization and dehydration of the three amino-acid residues alanine, serine and glycine [9]. The enzyme from some species is highly specific for phenylalanine [7,8].
PAL
Enzyme
Phenylalanine ammonia-lyase
Enzyme name
EC:4.3.1.24
Entry
Lyases;Carbon-nitrogen lyases;Ammonia-lyases
Class
L-phenylalanine ammonia-lyase (trans-cinnamate-forming)
Sysname
L-phenylalanine = trans-cinnamate + NH3[RN:R00697]
Reaction
trans-cinnamate [CPD:C00423];NH3 [CPD:C00014]
Product
Species information
Panax ginseng
Species name
Asiatic ginseng (Panax ginseng) is a species of eudicot in the family Araliaceae (ginseng family).
Species info
Gene sequence info
Panax_ginseng.PAL.fa
Download
>Pg_S1960.32
MLVRTNTLMQGYSGIRWELLEAMAKLINENLIPKLPLRGSITASGDLMPLSYITGILAGRHNSKEVTLEGAEVSAKEALKRAGISAPFELQAKEGLALVNGTAVGSAVAATVCFDANILSLLAEILSAFFCEAMHGKPEFTDPLTPELKHHPGQIESASIMKHLLDGSDYMKEAKIRHEKDPLTKPKQDRYALRTTPQWL
>Pg_S1960.33
MDNLRIAIAAIGKLIFAQFSELVCDHYNNGLPLNLSGGPNPSLYYGFKGAEIVMAAYSSELQYLANPVTTHVQSPEQHNQNMNSLGLISARKSAEAIEILKLMSATFMVALCQAIDLRHLEENMRENVKHAVLRAVRNSLFVDQDGSLLESHFCEKELLQVIEHHPVFAYINDTTNPCYAFLSQLREVLVEQALRDLKTEGMDKMGTRFSSEYWYLWRH
>Pg_S0085.45
MATNRIQKNFVPPFPTLPNAENDDPICLPPTHWIKAAESLQSSHFSEVQSMVSQFANYHTIGIQGTGLTVAQVTALARRTHLKVSLDEAVARERVAKSANWVADNISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGKEYLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAMTKLINENLVPKLPLRGTITASGDLVPLSYIAGVLTGRHNSKVVTVEGQEVSAMEALKRAGISAPFELQAKEGLALVNGTAVGSAVAATVCFDANIFSLLAEVLSALFCEAMHGKPEFTDPLTHELKHHPGQIESASIMKHLLDGSDYMKEAKIRHEKGPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSVEREINSVNDNPLIDVARDIALHGGNFQGTPIGVSMDNLRIALAAIGKLIFAQFSELVCDYYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSSTFLVALCQAIDLRHLEENMRETVKHVVLRAVRKTLYVDQDGSLLESRFCEKELLQVVEHHPVFSYIDDPTNPSYAFLAQLRGVVVEGALKDSKGEGMDENGYSIFKRIPVFMEELKECLGEEVVKSRERFENGDFKIENRIKKCRTYPIYKFVRSEVGTEILSGARKISPGEDIEKVYEAINEGKLGSVLMNCLSLAGGC
>Pg_S0024.2
MEALKRAGISAPFELQAKEGLALVNGTTVGSAMAATVCFDANIFSLLAEVLSELVCDYYNNGLPSNLSGVPNPSLDYGFKGAEIAMASYCSELQYLANPVTTHV
>Pg_S6037.3
MATNRIQKNFVPPFPTLPNAENDDPICLPPTHWIKAAESLQSSHFSEVQSMVSQFANYHTIGIQGTGLTVAQVTALARRTHLKVSLDEAVARERVAKSANWVADNISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGKEYLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAMTKLINENLVPKLPLRGTITASGDLVPLSYIAGVLTGRHNSKVVTVEGQEVSAMEALKRAGISVPFELQAKEGLALVNGTAVGSAVAATVCFDANIFSLLAEVLSALFCEAMHGKPEFTDPLTHELKHHPGQIESASIMKHLLDGSDYMKEAKIRHEKGPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSVEREINSVNDNPLIDVARDIALHGGNFQGTPIGVSMDNLRIALAAIGKLIFAQFSELVCDYSNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSSTFLVALCQAIDLRHLEENMRETVKHVVLRAVRKTLYVDQDGSLLESRFCEKELLQVIEHHPVFSYIDDPTNPSYAFLAQLRGVLVEGALKDSKGEGMDENGYSIFKRIPVFMEELKECLGEEVMKSRERFQNGDFKIENRIKKCRSYPIYKFVRSEVGTEILSGARKISPGEDIEKVYEAINEGKLGSVLMNCLSLAGGC
>Pg_S2940.7
MEALKRAGISAPFELQAKEGLALINGTAVGSAVAATVCFDANIFSLLAEVLSELVCDYYNNGLPSNLSGGPNPSLDYGFKGAEIAMALYCSELQYLANSVTTHVQSA
>Pg_S0406.8
MEALKRAGISAPFELQAKEGLALVNGTVVGSAVAATVCFDANIFSLLAEVLSELVCDYYNNGLPSNLSGGPNPSLDYGFKGAEIAMASYCSELQYLANPVTTHVQSA
>Pg_S1157.26
MKAAEALQSSHFSEVQSMVLQFEKYQTIDIQGTSLTVAQVTAIARRTNVKVCLDEAVACDRVAKSANWVADNISSLSTGFGATSNPRVTKTTDLQTELIRFLNAAIFHQVTPRLQCLSEQIP
>Pg_S1157.28
MDNLRIAIAAIGKLIFAQLSELVCDHYNNGLPLNLSGGPNPSLYYGFKGAEIVMAAYSSELQYLGNPVTTHVQSPEQHNQDMNSLGLISARKSAKAIEILKLMSATFMVALCQAINLRHLEENMRENVKHAVLGAVRNSLFVDQDGSLLESHFCEKELLQVIEHHPVFAYINDPTNPCYAFLSQLREVLVEQALRDLKTEGMDKMGTGFSSEYWYLWRH
>Pg_S0528.1
MEALKRAGISAPFELQAKEGLALVNEIAVGSTVAVTVKEMRKVPIARKTEVTWRQFSDAFILKFTEAGLDNLFEQFKSLKQSKDVNSY
MLVRTNTLMQGYSGIRWELLEAMAKLINENLIPKLPLRGSITASGDLMPLSYITGILAGRHNSKEVTLEGAEVSAKEALKRAGISAPFELQAKEGLALVNGTAVGSAVAATVCFDANILSLLAEILSAFFCEAMHGKPEFTDPLTPELKHHPGQIESASIMKHLLDGSDYMKEAKIRHEKDPLTKPKQDRYALRTTPQWL
>Pg_S1960.33
MDNLRIAIAAIGKLIFAQFSELVCDHYNNGLPLNLSGGPNPSLYYGFKGAEIVMAAYSSELQYLANPVTTHVQSPEQHNQNMNSLGLISARKSAEAIEILKLMSATFMVALCQAIDLRHLEENMRENVKHAVLRAVRNSLFVDQDGSLLESHFCEKELLQVIEHHPVFAYINDTTNPCYAFLSQLREVLVEQALRDLKTEGMDKMGTRFSSEYWYLWRH
>Pg_S0085.45
MATNRIQKNFVPPFPTLPNAENDDPICLPPTHWIKAAESLQSSHFSEVQSMVSQFANYHTIGIQGTGLTVAQVTALARRTHLKVSLDEAVARERVAKSANWVADNISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGKEYLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAMTKLINENLVPKLPLRGTITASGDLVPLSYIAGVLTGRHNSKVVTVEGQEVSAMEALKRAGISAPFELQAKEGLALVNGTAVGSAVAATVCFDANIFSLLAEVLSALFCEAMHGKPEFTDPLTHELKHHPGQIESASIMKHLLDGSDYMKEAKIRHEKGPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSVEREINSVNDNPLIDVARDIALHGGNFQGTPIGVSMDNLRIALAAIGKLIFAQFSELVCDYYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSSTFLVALCQAIDLRHLEENMRETVKHVVLRAVRKTLYVDQDGSLLESRFCEKELLQVVEHHPVFSYIDDPTNPSYAFLAQLRGVVVEGALKDSKGEGMDENGYSIFKRIPVFMEELKECLGEEVVKSRERFENGDFKIENRIKKCRTYPIYKFVRSEVGTEILSGARKISPGEDIEKVYEAINEGKLGSVLMNCLSLAGGC
>Pg_S0024.2
MEALKRAGISAPFELQAKEGLALVNGTTVGSAMAATVCFDANIFSLLAEVLSELVCDYYNNGLPSNLSGVPNPSLDYGFKGAEIAMASYCSELQYLANPVTTHV
>Pg_S6037.3
MATNRIQKNFVPPFPTLPNAENDDPICLPPTHWIKAAESLQSSHFSEVQSMVSQFANYHTIGIQGTGLTVAQVTALARRTHLKVSLDEAVARERVAKSANWVADNISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGKEYLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAMTKLINENLVPKLPLRGTITASGDLVPLSYIAGVLTGRHNSKVVTVEGQEVSAMEALKRAGISVPFELQAKEGLALVNGTAVGSAVAATVCFDANIFSLLAEVLSALFCEAMHGKPEFTDPLTHELKHHPGQIESASIMKHLLDGSDYMKEAKIRHEKGPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSVEREINSVNDNPLIDVARDIALHGGNFQGTPIGVSMDNLRIALAAIGKLIFAQFSELVCDYSNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSSTFLVALCQAIDLRHLEENMRETVKHVVLRAVRKTLYVDQDGSLLESRFCEKELLQVIEHHPVFSYIDDPTNPSYAFLAQLRGVLVEGALKDSKGEGMDENGYSIFKRIPVFMEELKECLGEEVMKSRERFQNGDFKIENRIKKCRSYPIYKFVRSEVGTEILSGARKISPGEDIEKVYEAINEGKLGSVLMNCLSLAGGC
>Pg_S2940.7
MEALKRAGISAPFELQAKEGLALINGTAVGSAVAATVCFDANIFSLLAEVLSELVCDYYNNGLPSNLSGGPNPSLDYGFKGAEIAMALYCSELQYLANSVTTHVQSA
>Pg_S0406.8
MEALKRAGISAPFELQAKEGLALVNGTVVGSAVAATVCFDANIFSLLAEVLSELVCDYYNNGLPSNLSGGPNPSLDYGFKGAEIAMASYCSELQYLANPVTTHVQSA
>Pg_S1157.26
MKAAEALQSSHFSEVQSMVLQFEKYQTIDIQGTSLTVAQVTAIARRTNVKVCLDEAVACDRVAKSANWVADNISSLSTGFGATSNPRVTKTTDLQTELIRFLNAAIFHQVTPRLQCLSEQIP
>Pg_S1157.28
MDNLRIAIAAIGKLIFAQLSELVCDHYNNGLPLNLSGGPNPSLYYGFKGAEIVMAAYSSELQYLGNPVTTHVQSPEQHNQDMNSLGLISARKSAKAIEILKLMSATFMVALCQAINLRHLEENMRENVKHAVLGAVRNSLFVDQDGSLLESHFCEKELLQVIEHHPVFAYINDPTNPCYAFLSQLREVLVEQALRDLKTEGMDKMGTGFSSEYWYLWRH
>Pg_S0528.1
MEALKRAGISAPFELQAKEGLALVNEIAVGSTVAVTVKEMRKVPIARKTEVTWRQFSDAFILKFTEAGLDNLFEQFKSLKQSKDVNSY
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University