Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product

Species information
Ostrya rehderiana
Species name
Ostrya rehderiana is a species of eudicot in the family Betulaceae (birch family).
Species info

Gene sequence info
Ostrya_rehderiana.HI4_OMT.fa
Download
>OreG0000650.1
MDIVHSQGATELFGVQSHLYKHIFSSSPLQVHIFSYIDSMPVNCAIELAIPDIIHSHGRPITLPQLASRLHIHPKKTSCLHMLMRLLVHSGFFTKTKLHHHHQYQPKDDYDDDEEEEGYALTPSSRLVIKDNVTSLSPFVKAMLDPVLVSPWHVLGDWLRGGSTQELTPFEKTHGMSIWSYCNQNTEYGGVFNEAMASDSQLMSFVVEDYKPIFEGLGSLVDVGGGTGTAARIISQAFPHVKCTVFDLPHFVANLPETPT
>OreG0000658.1
MRLLVHSGFFTKTKFHHHHQNQPEDDEEKEEEEEGYALTPSSRLLIKDNVTSMSPFVKAMLDPVLVSPWHVLGDWLRGGSTQELTPFEKTHGMSLLNYCNQNTEYGGVLNEAMASDSQLMSFVVEDYQPIFEGLGSLVDVGGGTGTVARIISQAFPHIECTVFDLPQVVANLPETTNLKYVGGDMFQSIPCADALLIKWILHDWSDEECVNILKRCREAITSKGRKGDHHRRGDK
>OreG0000651.1
MDIVRRREASELFEVQSYLHKHIFSYIDSMSLNCAIQLAIPDIIHSHGRPITLPQLASKLHIHPKKTSCLYRLMRLLVIKDNVNSLSPFVKAMLDPVLVSPWHVLGDWLREGSTQELTPFEKTHGMSLWNYCNQNTEYGGIFNEAMASNSQLLSFVVEDYKPIFEGLGSLVDVGGGTGTVARIISQAFPHIKCTVFDLPHVVANLPETTNLKYVGGDLFQSIPSADALLFKSILHDWSDEECVNILKRSREAITSKGKGGKVIIIDVVINEEEDEDDITKMKLFADTNLMVQLTGKERDKKEWEKLFFDAGFTHNNIVASYGIQSVIELYP
>OreG0000652.1
MEQIHGHGASELFQAQSHLYKHVFGFVGSMSLKCAVQLGIPDIIHSHGEPITLHGLVSALQIHPTKAGFVHRLMRLLAHCGFFATTRVNKKEIWSNLISGIIERKKACEVVNKSKMWA
>OreG0000654.1
MDIVHSQGANERIEVQSHVYKHIFSFIDSMSVNCAIQLAIPDIIHSHGRPITLPQLASKLHIHPQKTSCLYRLMRLLVHSGFFTKTKLHHHHHQNQPEDEEEGYALTPSSRLLIKDNVTSLSPFVKAMLDSALLSPWHVLGDWLREGSTQELTPFEKTHGMSLWNYCNQNTEYGGIFNEAMASNSQLLSFVVEDYKPIFEGLGSLVDVGGGNGTVARIISQAFPHIKCTVFDLPHVVANLPETTNLKYVGGDMFQSIPSADAILFKSVLHDWSDEECVNILKRCREAITSKGKGGKVIIIDVVINEEKDEDDVTKMKLHADTYMMVLLTGKERDKKEWEKLFFNAGFTHYNIVASHGMESVVELYP
>OreG0000656.1
MDIVHSREASELIEVQSHLHKHIFSFIDSMALNCAIQLAIPDIIHSHGRPITLPQLASKLHIHPKKTSCLYRLMRLLVHSGFFTKTKRHHHHHHQNQPEDEEKEEEEEGYALTPSSRLLIKDNVTSLSPFVKAVLDPVLVSPWRVLGDWLREGSTQELTPFEKTHGMSLWNYCSQNTEYGGIFNEAMASNSQLLSFVVEDYKPIFEGLGSLVDVGGGNGTVARIISQAFPHIKCTVFDLPHVVANLPETTNLKYVGGDMFQSIPSADAILFKSVLHDWSDEECVNILKRCREEVTSKGKGGKVIIIDVVINEEKDEDDITKNKLFADTYMMVLLTGKERDKKEWEKLFFNAGFTHYNIVASHGVESVIELYP
>OreG0000660.1
MEQIHGHGASELFQAQSHLYKHVFGFVGSMSLKCAVQLGIPDIIHSHGKPITLHGLVSALQIHPTKAGFVLRLMRLLAHCGFFATTRVNKNQEEEEEEEAYDLTPSSRILLKDNVTSLSPFVLAMLDPALVTPWQFLGNWFREDKVTAFESAHGMGFWEYGNQNPGFNNLFNEAMASDSGMMNLVVRDCKAVFEGLDTLIDVGGGTGTCSRIISEAFPNLKCTVFDLPHVVADLTDSLKLNYVGGDMFQFIPPTEAILLKLVLHSISDEDCVKVLKKCREAILNKKKGGKVIIIDIVINDIKDEHKITEAKLFFDVLMMALVTGRERCEKEWEKLFLEAGFSHYKITPMFGLRSLIEVYP
MDIVHSQGATELFGVQSHLYKHIFSSSPLQVHIFSYIDSMPVNCAIELAIPDIIHSHGRPITLPQLASRLHIHPKKTSCLHMLMRLLVHSGFFTKTKLHHHHQYQPKDDYDDDEEEEGYALTPSSRLVIKDNVTSLSPFVKAMLDPVLVSPWHVLGDWLRGGSTQELTPFEKTHGMSIWSYCNQNTEYGGVFNEAMASDSQLMSFVVEDYKPIFEGLGSLVDVGGGTGTAARIISQAFPHVKCTVFDLPHFVANLPETPT
>OreG0000658.1
MRLLVHSGFFTKTKFHHHHQNQPEDDEEKEEEEEGYALTPSSRLLIKDNVTSMSPFVKAMLDPVLVSPWHVLGDWLRGGSTQELTPFEKTHGMSLLNYCNQNTEYGGVLNEAMASDSQLMSFVVEDYQPIFEGLGSLVDVGGGTGTVARIISQAFPHIECTVFDLPQVVANLPETTNLKYVGGDMFQSIPCADALLIKWILHDWSDEECVNILKRCREAITSKGRKGDHHRRGDK
>OreG0000651.1
MDIVRRREASELFEVQSYLHKHIFSYIDSMSLNCAIQLAIPDIIHSHGRPITLPQLASKLHIHPKKTSCLYRLMRLLVIKDNVNSLSPFVKAMLDPVLVSPWHVLGDWLREGSTQELTPFEKTHGMSLWNYCNQNTEYGGIFNEAMASNSQLLSFVVEDYKPIFEGLGSLVDVGGGTGTVARIISQAFPHIKCTVFDLPHVVANLPETTNLKYVGGDLFQSIPSADALLFKSILHDWSDEECVNILKRSREAITSKGKGGKVIIIDVVINEEEDEDDITKMKLFADTNLMVQLTGKERDKKEWEKLFFDAGFTHNNIVASYGIQSVIELYP
>OreG0000652.1
MEQIHGHGASELFQAQSHLYKHVFGFVGSMSLKCAVQLGIPDIIHSHGEPITLHGLVSALQIHPTKAGFVHRLMRLLAHCGFFATTRVNKKEIWSNLISGIIERKKACEVVNKSKMWA
>OreG0000654.1
MDIVHSQGANERIEVQSHVYKHIFSFIDSMSVNCAIQLAIPDIIHSHGRPITLPQLASKLHIHPQKTSCLYRLMRLLVHSGFFTKTKLHHHHHQNQPEDEEEGYALTPSSRLLIKDNVTSLSPFVKAMLDSALLSPWHVLGDWLREGSTQELTPFEKTHGMSLWNYCNQNTEYGGIFNEAMASNSQLLSFVVEDYKPIFEGLGSLVDVGGGNGTVARIISQAFPHIKCTVFDLPHVVANLPETTNLKYVGGDMFQSIPSADAILFKSVLHDWSDEECVNILKRCREAITSKGKGGKVIIIDVVINEEKDEDDVTKMKLHADTYMMVLLTGKERDKKEWEKLFFNAGFTHYNIVASHGMESVVELYP
>OreG0000656.1
MDIVHSREASELIEVQSHLHKHIFSFIDSMALNCAIQLAIPDIIHSHGRPITLPQLASKLHIHPKKTSCLYRLMRLLVHSGFFTKTKRHHHHHHQNQPEDEEKEEEEEGYALTPSSRLLIKDNVTSLSPFVKAVLDPVLVSPWRVLGDWLREGSTQELTPFEKTHGMSLWNYCSQNTEYGGIFNEAMASNSQLLSFVVEDYKPIFEGLGSLVDVGGGNGTVARIISQAFPHIKCTVFDLPHVVANLPETTNLKYVGGDMFQSIPSADAILFKSVLHDWSDEECVNILKRCREEVTSKGKGGKVIIIDVVINEEKDEDDITKNKLFADTYMMVLLTGKERDKKEWEKLFFNAGFTHYNIVASHGVESVIELYP
>OreG0000660.1
MEQIHGHGASELFQAQSHLYKHVFGFVGSMSLKCAVQLGIPDIIHSHGKPITLHGLVSALQIHPTKAGFVLRLMRLLAHCGFFATTRVNKNQEEEEEEEAYDLTPSSRILLKDNVTSLSPFVLAMLDPALVTPWQFLGNWFREDKVTAFESAHGMGFWEYGNQNPGFNNLFNEAMASDSGMMNLVVRDCKAVFEGLDTLIDVGGGTGTCSRIISEAFPNLKCTVFDLPHVVADLTDSLKLNYVGGDMFQFIPPTEAILLKLVLHSISDEDCVKVLKKCREAILNKKKGGKVIIIDIVINDIKDEHKITEAKLFFDVLMMALVTGRERCEKEWEKLFLEAGFSHYKITPMFGLRSLIEVYP
Gene tree
Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University