Enzyme

Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.

HI4'OMT

Enzyme

Isoflavone 4'-O-methyltransferase

Enzyme name

EC:2.1.1.46

Entry

Transferases;Transferring one-carbon groups;Methyltransferases

Class

S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase

Sysname

S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]

Reaction

S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]

Product

Species information

Oryza sativa

Species name

Asian cultivated rice (Oryza sativa) is a species of monocot in the family Poaceae (grass family).

Species info

Gene sequence info

Oryza_sativa.HI4_OMT.fa

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>rna-XM_015783065.2
MGSLELSKNKCNGGQEVSLLDAQLELYSNTFAVIKSMALKSALDLGIADAVHRHGGAATLAEIASEVALHPSKIPCLRRLMRALTVSGVFAAAVKPGDGGGGEPVYELTPSSRLLVGSSNLSGIMSMILHPTLVVPFLGVGEWLRRDREPPEEDPYCIFKQAHGRSLWELAGRDAAFDALINDGMVSDSRVIMDYVVREHGEVFRGIASLVDLAGGLGAAAQVISKAFPEVRCSVMDLGHVVAKAPAGTDVEYIAGDMFESVPPADAVFLKWVLHDWGDNDCIKILKNCKKSITPRDKGGKVIIMDIVVGAGPSDQKHREVQALFDMYIMLVNGIERDEQEWKKVFVEAGFSGYKIMPILGFRSMIEVYP
>rna-XM_015782901.2
MELTESKCDGGQEVSLLDAQLELYWNTFAVIKSMALKSALDLRIADAVHLHGGAATLAEIASEVALHPSKIPCLRRLMRALTVSGVFAAAVKPGDGGGGEPVYELTPSSRLLVGSSNLSGIMSMILHPTLVVPFLGVGEWLRRDREPPEEDPYCIFKQAHGRSLWELAGRDAAFDALINDGMVSDSRVIMDYVVREHGEVFRGIASLVDLAGGLGAAAQVISKAFPEVRCSVMDLGHVVAKAPAGTDVEYIAGDMFESVPPADAVFLKWVLHDWGDDDCIKILKNCKKAIPPRDKGGKVIIMDIVVGAGPSDQKHREVQALFDMYIMFVNGIERDEQEWKKVFMGAGFSGYKIMPVLGFRSMIEVYP
>rna-XM_015787174.2
MHEHTTQHSYEMSTEELLQGHLQLYHHFFSYIKSMALKCAAELGIPAAIHRRGGAATLRDIVADVALRQAKVPHLRRLMRVLTVSGIFAMKQQQPASSGEAVYTLTPASRLLVAGAGGGHDMSPMLRFLVHPTALTPFFSLHAWFRVDDEEEEEEPVAAGGGAAMSLFEMAHGFPRWEMTGRDAAYGAVLNDAMAADSRFVMEVVFREGGGDVFRGIGSLVDVGGGHGAAAAAVAAAFPHVKCSVLDLPQVVRKAPPDAGDVRFVAGDMFEYVPPADAVLLKYVLHCFGDDDCVKILRRCKEAIPARDAGGKVIIINMVIGSGSQRDIFKETQVLFDLYMMYIDGVEREEKEWENIFSKAGFSAYKIMPILGFLSIIEVYP
>rna-XM_015789584.2
MEHEQLVQASTELMHHSLGYVRSMALGCAAKLGVADAIHRAGGRATLHDLHAALSLHPTKLPFLRRVMRVLVASGVFAQVKEEEDHYRLTPVSSLLVTAGRTLLPFVLLQHSPLCVTPATSMAEWLKTGEEETAFEMAHGAGLWGACRRAPELGDFFNDAMAADSAFIMDAAIRGARQVFDKITSLVDVAGGTGAAARAVAAAFPHIKCTVLDLPHVIDSIPVDHGDVVQFVAGDMMDFIPQADALLLKFVLHDWSDEDCVKILKRCKEAIPSKDKGGKVIIIDVVVGSSSQAMCYGTQLLFDLTISMLTPGMERDEKEWFKIFNEAGFTEYKISPVLGIRSIIEVFP
>rna-XM_015789878.2
MEHEQLLQASTELMNHSLGYIRSMALGCAAKLGVADAIHHAGGRATMDDLRAALSLHPSKLPYFLRRVMRVLVASGVFAHDEEEDDDDIYRVTPVSSLLVTATGGNGGRSLLPFVLLQLSPPIYVTPATSMAEWLTSGEEETPFEMTHGAGLWTVCSRDPELGELFNDAMAADSAFIMDVAIRGAGRQVFDKITSLVDVAGGTGTAARVVAAAFPHIKCTVLDLPHVIDSIPADHRGRDVVKFVAGDMMDFIPRADALLLKFVLHDWSDEDCMKILKRCKEAIPSREAGGKVIVIDVVVGSSTQAMCHGTQLLFDLLISTTLPGMQRGEKEWCKVFKEAGFTDYKISPVLGIRSIIEVFP
>rna-XM_015789585.2
MEHEQLVQASTELMHHSLGYVRSMALGCAAKLGVADAIHRAGGRATLHDLHAALSLHPTKLPFLRRVMRVLVASGVFAQVKEEEDHYRLTPVSSLLVTAGRTLLPFVLLQHSPLCVTPATSMAEWLKTGEEETAFEMAHGAGLWGACRRAPELGDFFNDAMAADSAFIMDAAIRGARQVFDKITSLVDVAGGTGAAARAVAAAFPHIKCTVLDLPHVIDSIPVDHGDVVQFVAGDMMDFIPQADALLLKFVLHDWSDEDCVKILKRCKEAIPSKDKGGKVIIIDVVAWRGMRKSGSRYSTRPGSLSTRLVLC
>rna-XM_015755512.2
MAQNVQENEQVMSTEDLLQAQIELYHHCLAFIKSMALRAATDLRIPDAIHCNGGAATLTDLAAHVGLHPTKLSHLRRLMRVLTLSGIFTVHDGDGEATYTLTRVSRLLLSDGVERTHGLSQMVRVFVNPVAVASQFSLHEWFTVEKAAAVSLFEVAHGCTRWEMIANDSKDGSMFNAGMVEDSSVAMDIILRKSSNVFRGINSLVDVGGGYGAVAAAVVRAFPDIKCTVLDLPHIVAKAPSNNNIQFVGGDLFEFIPAADVVLLKCILHCWQHDDCVKIMRRCKEAISARDAGGKVILIEVVVGIGSNETVPKEMQLLFDVFMMYTDGIEREEHEWKKIFLEAGFSDYKIIPVLGVRSIIEVYP
>rna-XM_015755511.2
MAQNVQENEQVMSTEDLLQAQIELYHHCLAFIKSMALRAATDLRIPDAIHCNGGAATLTDLAAHVGLHPTKLSHLRRLMRVLTLSGIFTVHDGDGEATYTLTRVSRLLLSDGVERTHGLSQMVRVFVNPVAVASQFSLHEWFTVEKAAAVSLFEVAHGCTRWEMIANDSKDGSMFNAGMVEDSSVAMDIILRKSSNVFRGINSLVDVGGGYGAVAAAVVRAFPDIKCTVLDLPHIVAKAPSNNNIQFVGGDLFEFIPAADVVLLKCILHCWQHDDCVKIMRRCKEAISARDAGGKVILIEVVVGIGSNETVPKEMQLLFDVFMMYTDGIEREEHEWKKIFLEAGFSDYKIIPVLGVRSIIEVYP
>rna-XM_015757665.2
MAQRVQEEDEQMMSTDDLIQAQIKLYHHCFAFIKSTALWAAIDLRIADVIHRNGGAATLSDLALNVGLHPTKLSHLRRLMRVLTVTGIFAVEDRNGEAMYTLTRVSRLLLNSDGEGTHALSQMARVLANPLAVISHFSIHEWFTTEKATTMTPFEVAHGCTRWEMIANDAKDGSVFNAGMVEDSRVAMDIILKESCGIFQGISSLIDVGGGHGAAAAAIATAFPNIKCTVLDLPHIVAEAPATHSNIQFIGGDLFKFIPAADVVLLKCLLHCWQDDDCVKILRLCKEAIPARDAGGKVIIIEVVVGIGSEEIVPKEMQLLFDVFMMYIDGIEREEYEWKKIFLEAGFSDYKITPVLGARSIIEVYP
>rna-XM_015757837.2
MAQRVQEEDEQMTSTDDLIQAEIELYHHCFAFIKSTALRAATDLCISDAIHRNGGAATLSDLALNIGLHPTKLSHLRRLMRVPTVSGVFAVEDHNGEAMYTLTRVSRLLLNGDGERTHALSHLVRVLVNPLTVASHFSIHEWFTIEQAAAMTPFEVAHGCTRWEIIANDAKDGSVFNTAMVEDSRVAMDIILKESCGVFQGISSLVDVGGGHGAAAAAIATAFPNIKCTVLDLPHIVAEAPTTHSNIQFVGGDFFEFIPAADVVLLKYILHAWQDDDCVKILRRCKEAILARDAGGKVIIIEVVVGIGPKEIVPKEMQILFDVFMMYVDGIEREEHEWKKIFLEAGFSDYKITPVLGARSIIEVYP
>rna-XM_015761109.2
MAGQPPNMLAPPTDDELLHAQADMLRHSLYFVTSMAFQCAVKLGIPTAIHRAGGTASLPDLVAALSLPPAKLPFLRRLMRLLVNSGVFAAADDTATGWAGMYRLTPLSWLLVEGEGAAPVVDGHPSQVLVVLAATSRHFVEAAMGLADWFGKDLPPSSSAPPSPFEEVHGAALFDESMANLDPESDSMFNEALAAHDHSDFPTVLRECREVFQGVESLTDCRGGDGRAAKAIVEAFPHIKFTVLDFPRVIGETRTGVVNYVAGDMFREIPPAQAVMLQLVLHHWNDEDCVKILANCKKAIPTREDGGKVIIIDIVIGAPSGLLLEAQLLMDVAMMVVTKGRQRDENDWRDLFSKAGFSDYNIVKKLGARGVFEVYP
>rna-XM_026022261.1
MAHAKSNGYQASDATSSLDALRELYGNTFAVVKSMALKAAMDLGIADAIHHHGGAATLSQIVTRVALHPSKVPCLRRLMRVLTLSGVFAVQKLAPGDAAAPADEAEAAVYALTPVSRLLIGAGNQGHMMSMLLHPNFITPFFRISDWLQRELPGPCIFKHTHGRSLWEMADDDAAFNTVVNDGMASDSIFTMDILVREHGEVFQGISSLVDVAGGNGTAAQAIARAFPEVKCSVMDLAHVVAEAPGGTGVEFITGDMFESVPPANAVFLKWIMHD*GDNDCVKILGNCKKAIPTRDKGEKVIIMDIVVGTGPSDQKHRDVQILYDAYIMFINGAERDEQEWKKLFLEAGFSDYKIMPIMGFRSIIEVYP
>rna-XM_015764071.2
MATTQSNDHGAGLLDAQLELYANTLAVVKSMALKTAMDLGIADAIHHHGGAATLPQILTRVTLHPSKIPCLRRLMRVLTLTGVFAVEKPTAADEPPVYALTPVSRLLVSSGNLQQAPIMSLLLHPSCITPFLRIGDWLQRELPGSSIFEHTHGRSLWEVADGDAAFSKVFNDAMVSDSRLVMDVVVREHGDVFRRISSLVDVAGGHGTAAQAIARAFPEVKCSVMDLAHVVAKAPGGTGVEYIAGDMFESIPPANAVFLKWIMHDWGDDECVKVLKNAKKAIPSKDAGGKVIIIDVVVRAGSPDQKHIELQALFGAYMMLINGVERDEKEWKKVFIEAGFSGYKIIPVLGFRSIIEVYP

Gene tree

Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University