Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Oryza rufipogon
Species name
Common wild rice (Oryza rufipogon) is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Oryza_rufipogon.HI4_OMT.fa
Download
>R1005829.t1
MELTESKCDGGQEVSLLDAQLELYWNTFAVIKSMALKSALDLRIADAVHRHGGAATLAEIASEVALHPSKIPCLRRLMRALTVSGVFAAAVKPGDGGGGEPVYELTPSSRLLVGSSNLSGIMSMILHPTLVVPFLGVGEWLRRDREPPEEDPYCIFKQAHGRSLWELAGRDAAFDALVNDGMVSDSRVIMDYVVREHGEVFRGIASLVDLAGGLGAAAQVISKAFPEVRCSVMDLVHVVAKAPAGTDVEYIAGDMFESVPPADAVFLKWVLHDWGDDDCIKILKNCKKSIPPRDKGGKVIIMDIVVGAGPSDQKHREVQALFDMYIMLVNGIERDEQEWKKVFVEAGFSGYKIMPILGFRSMIEVYP
>R1005830.t1
MGSLELSESKCNGGQEVSLLDAQLELYWNTFAVIKSMALKSALDLGIADAVHRHGGAATLAEIAGEVALHPSKIPCLRRLMRALTVSGVFAAAVKPGDGGGGEPVYELTPSSRLLAGSSTLSGIMSMILHPTLVVPFLGVGEWLRRDREPPEEDPYCIFKQAHGRSLWELAGRDAAFDALVNDGMVSDSRVIMDYVVREHGEVFRGIASLVDLAGGLGAAAQVISKAFPEVRCSVMDLVHVVAKAPAGTDVEYIAGDMFESVPPADAVFLKWVLHDWGDDDCIKILKNCKKSIPPRDKGGKVIIMDIVVGAGPSDQKHREVQALFDMYIMLVNGIERDEQEWKKVFVEAGFSGYKIMPILGFRSMIEWVLHDWSDDDCIKILKNCKKAIPPRDKGGKVIIMDIVVGAGPSDQKHREVQALFDMYIMFVNGIERDEQEWKKVFMGAGFSGYKIMPVLGFRSMIEVYP
>R1023660.t1
MAGQPPNMLAPPTDDELLHAQADLWRHSLYFVTSMAFQCAVKLGIPTAIHRAGGTASLADLVAALSLPPAKLPFLRRLMRLLVNSGVFAAADDTATGWAGMYRLTPLSWLLVEGEGAAPVVDGHPSQVLVVLAATSRHCVEAAMGLADWIGKDLPPSSSAPPSPFEEGES
>R1023658.t1
MAAQAPTMLAPTNEELLQAQADLWRHSLYYITSLSLQCVIKLGIPTAIHRAGGAASLPDLVTALSLPPSKLPFLRRLMRLLVHSGVFAADDTTDTGTYRLTPLSCLLVPTVLAMGSRHCVEAAMGLADWIKKDSPSLLFEDVHGATPFDESMADLDPETHGLLNEGLAAHDGSGFAAVLRECREVFQGLESLTDCGGGDGATARTIVEAFPHIKCTVLDLPRVIGDKKTDGVVSYVAGDMLSFIPPAQAVMLKVVLHHWSDQDCVKILAHCKKAIPSREAGGKVIIIDVVISSTSGPLLEAELLMDVGMILIGKGQQRDENKWCDLFKKAGFSDYKTVKKLGIRGIFEVYP
>R1001367.t1
MATTQSNDHGAGLLDAQLELYANTLAVVKSMALKTAMDLGIADAIHHHGGAATLPQILTRVTLHPSKMPCLRRLMRVLTLTGVFAVEKPTAADEPPVYALTPVSRLLVSSGNLQQAPIMSLLLHPSCITPFLRIGDWLQRELPGSSIFEHTHGRSLWEVADGDAAFSKVFNDAMVSDSRLVMDVVVREHGEVFRGISSLVDVAGGHGTAAQAIARAFPEVKCSVMDLAHVVAKAPGGTGVEYIAGDMFESIPPANAVFLKWIMHDWGDDECVKVLKNAKKAIPSKDAGGKVIIIDVVVGAGPPDQKHIELQALFGAYMMLINGVERDEKEWKKVFIEAGFSGYKIIPVLGFRSIIEVYP
>R1027248.t1
MVGAFALLYHHVFSYIKPMALKCAVELGIPDAIHRRGGAATLADIAADTGVHASRLTDLRCLMRLLTTSGMFAATAAATDGDEGEPAVATSTMYTLTAASGLVVGPRGLSTVVRFAAGPFAVSPFFDMHAWVRAAPAAAPPAARSLFELAHGRSRWDAANADNDTMKAHSFVESQLLIEAVLRDHAGVFRGLASLVDVGGGHGAVAKAIAAAFPDIKCTVMDLPHVVADAPVSDDGNLHLVAGDMFQSIPPADAVLLKYVLHCWGDDDCVRILRNCREAIPAREAGGKVIITELVLGSSAAPRDANVAEAEDMHSLFLMCISGVGREEREWRAIFSDAGFGDYKITPVLGPISVIEVYP
>R1033616.t1
MAHAKSNGYHASDATSSLDALSELYGNTFAVVKSMALKAAMDLGIADAIHHHGGAATLSQIVTRVALHPSKVPCLRRLMRVLTLSGVFAVQKLAPGDAAAPADEAEAAVYALTPVSRLLIGAGNQGHMMSMLLHPNFITPFFRISDWLQRELPGPCIFKHTHGRSLWEMADDDAAFNTVVNDGMASDSIFTMDILVREHGEVFQGISSLVDVAGGNGTAAQAIARAFPEVKCSVMDLAHVVAEAPGGTGVEFIAGDMFESVPPANAVFLKWIMHDWGDNDCVKILGNCKKAIPTRDKGGKVIIMDIVVGTGPSDQKHRDVQILYDAYILFINGAERDEQEWKKLFLEAGFSDYKIMPIMGFRSIIEVYP
>R1013384.t1
MANLDPESDSMFNEALAAHDHSGFPTVLRECREVFQGVESLTDCRGGDGRAAKAIVEAFPHIKCTVLDFPRVIGETRTGVVNYVAGDMFREIPPAQAVMLQLVLHHWNDEDCVKILANCKKAIPTREDGGKVIIIDIVIGAPSGLLLEAQLLMDVAMMVVTKGRQRDENDWRDLFSKAGFSDYNIVKKLGARGVFEVYP
>R1020261.t1
MSTEELLQGHLQLYHHFFSYIKSMALKCAAELGIPAAIHRRGGAATLRDIVADVALRQAKVPHLRRLMRVLTVSGIFAMKQQQPASSGEAVYTLTPASRLLVAGAGGGHDMSPMLRFLVHPTALTPFFSLHAWFRVDDEEEEEEPVAAGGGGGGAAMSLFEMAHGFPRWEMTGRDAAYGAVLNDAMAADSRFVMEVVFREGGGDVFRGIGSLVDVGGGHGAAAAAVAAAFPHVKCSVLDLPQVVRKAPPDAGDVRFVAGDMFEYVPPADAVLLKYVLHCFGDDDCVKILRRCKEAIPARDAGGKVIIINMVIGSGSQRDIFKETQVLFDLYMMYIDGVEREEKEWENIFSKAGFSAYKIMPILGFLSIIEVYP
>R1000696.t1
MEHEQLLQASTELMNLGYIRSMALGCAAKLGVADAIHHAGGRATMDDLRAALSLHPSKLPYFLRRVMRVLVASGVFAHDEEEDDDDIYRLTPVSSLLVTATGGNGGRSLLPFVLLQLSPPLYVTPATSMAEWLTSGEEETPFEMTHGAGLWTVCSRDPELGELFNDAMAADSAFIMDVAIRGAGRQVFDKITSLVDVAGGTGTAARVVAAAFPHIKCTVLDLPHVIDSIPADHRGRDVVKFVAGDMMDFIPRADALLLNFVLHDWSDEDCMKILKRCKEAIPSREAGGKVIVIDVVVGSSTQAMCHGTQLLFDLLISTTLPGMQRGEKEWCKVFKEAGFTDYKISPVLGIRSIIEVFP
>R1000693.t2
MEHEQLVQASTELMHHSLGYVRSMALGCAAKLGVADAIHRAGGRATLHDLHAALSLHPTKLPFLRRVMRVLVASGVFAQVKEEEEDHYRLTPVSSLLVTAGRTLLPFVLLQHSPLCVTPATSMAEWLKTGEEETAFEMAHGAGFWGACRRAPELGDFFNDAMAADSAFIMDAAIRGARQVFDKITSLVDVAGGTGAAARAVAAAFPHIKCTVLDLPHVIDSIPVDHGDVVQFVAGDMMDFIPQADALLLKSVLHDWSDEDCVKILKRCKEAIPSKDTGGKVIIIDVVVGSSSQAMCYGTQLLFDLTISMLTPGMERDEKEWFKIFNEAGFTEYKISPVLGIRSIIEVFP
MELTESKCDGGQEVSLLDAQLELYWNTFAVIKSMALKSALDLRIADAVHRHGGAATLAEIASEVALHPSKIPCLRRLMRALTVSGVFAAAVKPGDGGGGEPVYELTPSSRLLVGSSNLSGIMSMILHPTLVVPFLGVGEWLRRDREPPEEDPYCIFKQAHGRSLWELAGRDAAFDALVNDGMVSDSRVIMDYVVREHGEVFRGIASLVDLAGGLGAAAQVISKAFPEVRCSVMDLVHVVAKAPAGTDVEYIAGDMFESVPPADAVFLKWVLHDWGDDDCIKILKNCKKSIPPRDKGGKVIIMDIVVGAGPSDQKHREVQALFDMYIMLVNGIERDEQEWKKVFVEAGFSGYKIMPILGFRSMIEVYP
>R1005830.t1
MGSLELSESKCNGGQEVSLLDAQLELYWNTFAVIKSMALKSALDLGIADAVHRHGGAATLAEIAGEVALHPSKIPCLRRLMRALTVSGVFAAAVKPGDGGGGEPVYELTPSSRLLAGSSTLSGIMSMILHPTLVVPFLGVGEWLRRDREPPEEDPYCIFKQAHGRSLWELAGRDAAFDALVNDGMVSDSRVIMDYVVREHGEVFRGIASLVDLAGGLGAAAQVISKAFPEVRCSVMDLVHVVAKAPAGTDVEYIAGDMFESVPPADAVFLKWVLHDWGDDDCIKILKNCKKSIPPRDKGGKVIIMDIVVGAGPSDQKHREVQALFDMYIMLVNGIERDEQEWKKVFVEAGFSGYKIMPILGFRSMIEWVLHDWSDDDCIKILKNCKKAIPPRDKGGKVIIMDIVVGAGPSDQKHREVQALFDMYIMFVNGIERDEQEWKKVFMGAGFSGYKIMPVLGFRSMIEVYP
>R1023660.t1
MAGQPPNMLAPPTDDELLHAQADLWRHSLYFVTSMAFQCAVKLGIPTAIHRAGGTASLADLVAALSLPPAKLPFLRRLMRLLVNSGVFAAADDTATGWAGMYRLTPLSWLLVEGEGAAPVVDGHPSQVLVVLAATSRHCVEAAMGLADWIGKDLPPSSSAPPSPFEEGES
>R1023658.t1
MAAQAPTMLAPTNEELLQAQADLWRHSLYYITSLSLQCVIKLGIPTAIHRAGGAASLPDLVTALSLPPSKLPFLRRLMRLLVHSGVFAADDTTDTGTYRLTPLSCLLVPTVLAMGSRHCVEAAMGLADWIKKDSPSLLFEDVHGATPFDESMADLDPETHGLLNEGLAAHDGSGFAAVLRECREVFQGLESLTDCGGGDGATARTIVEAFPHIKCTVLDLPRVIGDKKTDGVVSYVAGDMLSFIPPAQAVMLKVVLHHWSDQDCVKILAHCKKAIPSREAGGKVIIIDVVISSTSGPLLEAELLMDVGMILIGKGQQRDENKWCDLFKKAGFSDYKTVKKLGIRGIFEVYP
>R1001367.t1
MATTQSNDHGAGLLDAQLELYANTLAVVKSMALKTAMDLGIADAIHHHGGAATLPQILTRVTLHPSKMPCLRRLMRVLTLTGVFAVEKPTAADEPPVYALTPVSRLLVSSGNLQQAPIMSLLLHPSCITPFLRIGDWLQRELPGSSIFEHTHGRSLWEVADGDAAFSKVFNDAMVSDSRLVMDVVVREHGEVFRGISSLVDVAGGHGTAAQAIARAFPEVKCSVMDLAHVVAKAPGGTGVEYIAGDMFESIPPANAVFLKWIMHDWGDDECVKVLKNAKKAIPSKDAGGKVIIIDVVVGAGPPDQKHIELQALFGAYMMLINGVERDEKEWKKVFIEAGFSGYKIIPVLGFRSIIEVYP
>R1027248.t1
MVGAFALLYHHVFSYIKPMALKCAVELGIPDAIHRRGGAATLADIAADTGVHASRLTDLRCLMRLLTTSGMFAATAAATDGDEGEPAVATSTMYTLTAASGLVVGPRGLSTVVRFAAGPFAVSPFFDMHAWVRAAPAAAPPAARSLFELAHGRSRWDAANADNDTMKAHSFVESQLLIEAVLRDHAGVFRGLASLVDVGGGHGAVAKAIAAAFPDIKCTVMDLPHVVADAPVSDDGNLHLVAGDMFQSIPPADAVLLKYVLHCWGDDDCVRILRNCREAIPAREAGGKVIITELVLGSSAAPRDANVAEAEDMHSLFLMCISGVGREEREWRAIFSDAGFGDYKITPVLGPISVIEVYP
>R1033616.t1
MAHAKSNGYHASDATSSLDALSELYGNTFAVVKSMALKAAMDLGIADAIHHHGGAATLSQIVTRVALHPSKVPCLRRLMRVLTLSGVFAVQKLAPGDAAAPADEAEAAVYALTPVSRLLIGAGNQGHMMSMLLHPNFITPFFRISDWLQRELPGPCIFKHTHGRSLWEMADDDAAFNTVVNDGMASDSIFTMDILVREHGEVFQGISSLVDVAGGNGTAAQAIARAFPEVKCSVMDLAHVVAEAPGGTGVEFIAGDMFESVPPANAVFLKWIMHDWGDNDCVKILGNCKKAIPTRDKGGKVIIMDIVVGTGPSDQKHRDVQILYDAYILFINGAERDEQEWKKLFLEAGFSDYKIMPIMGFRSIIEVYP
>R1013384.t1
MANLDPESDSMFNEALAAHDHSGFPTVLRECREVFQGVESLTDCRGGDGRAAKAIVEAFPHIKCTVLDFPRVIGETRTGVVNYVAGDMFREIPPAQAVMLQLVLHHWNDEDCVKILANCKKAIPTREDGGKVIIIDIVIGAPSGLLLEAQLLMDVAMMVVTKGRQRDENDWRDLFSKAGFSDYNIVKKLGARGVFEVYP
>R1020261.t1
MSTEELLQGHLQLYHHFFSYIKSMALKCAAELGIPAAIHRRGGAATLRDIVADVALRQAKVPHLRRLMRVLTVSGIFAMKQQQPASSGEAVYTLTPASRLLVAGAGGGHDMSPMLRFLVHPTALTPFFSLHAWFRVDDEEEEEEPVAAGGGGGGAAMSLFEMAHGFPRWEMTGRDAAYGAVLNDAMAADSRFVMEVVFREGGGDVFRGIGSLVDVGGGHGAAAAAVAAAFPHVKCSVLDLPQVVRKAPPDAGDVRFVAGDMFEYVPPADAVLLKYVLHCFGDDDCVKILRRCKEAIPARDAGGKVIIINMVIGSGSQRDIFKETQVLFDLYMMYIDGVEREEKEWENIFSKAGFSAYKIMPILGFLSIIEVYP
>R1000696.t1
MEHEQLLQASTELMNLGYIRSMALGCAAKLGVADAIHHAGGRATMDDLRAALSLHPSKLPYFLRRVMRVLVASGVFAHDEEEDDDDIYRLTPVSSLLVTATGGNGGRSLLPFVLLQLSPPLYVTPATSMAEWLTSGEEETPFEMTHGAGLWTVCSRDPELGELFNDAMAADSAFIMDVAIRGAGRQVFDKITSLVDVAGGTGTAARVVAAAFPHIKCTVLDLPHVIDSIPADHRGRDVVKFVAGDMMDFIPRADALLLNFVLHDWSDEDCMKILKRCKEAIPSREAGGKVIVIDVVVGSSTQAMCHGTQLLFDLLISTTLPGMQRGEKEWCKVFKEAGFTDYKISPVLGIRSIIEVFP
>R1000693.t2
MEHEQLVQASTELMHHSLGYVRSMALGCAAKLGVADAIHRAGGRATLHDLHAALSLHPTKLPFLRRVMRVLVASGVFAQVKEEEEDHYRLTPVSSLLVTAGRTLLPFVLLQHSPLCVTPATSMAEWLKTGEEETAFEMAHGAGFWGACRRAPELGDFFNDAMAADSAFIMDAAIRGARQVFDKITSLVDVAGGTGAAARAVAAAFPHIKCTVLDLPHVIDSIPVDHGDVVQFVAGDMMDFIPQADALLLKSVLHDWSDEDCVKILKRCKEAIPSKDTGGKVIIIDVVVGSSSQAMCYGTQLLFDLTISMLTPGMERDEKEWFKIFNEAGFTEYKISPVLGIRSIIEVFP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University