Enzyme

Isolated from the plant Gentiana triflora. Transfers the hydroxycinnamoyl group only to the C-5 glucoside of anthocyanin. Caffeoyl-CoA, but not malonyl-CoA, can substitute as an acyl donor.

5AT

Enzyme

Anthocyanin 5-aromatic acyltransferase

Enzyme name

EC:2.3.1.153

Entry

Transferases;Acyltransferases;Transferring groups other than aminoacyl groups

Class

4-hydroxycinnamoyl-CoA:anthocyanidin 3,5-di-O-beta-D-glucoside 5-O-glucoside-6'''-O-4-hydroxycinnamoyltransferase

Sysname

4-hydroxycinnamoyl-CoA + an anthocyanidin 3,5-di-O-beta-D-glucoside = CoA + anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [RN:R05163]

Reaction

CoA [CPD:C00010];anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [CPD:C06363]

Product

Species information

Ananas comosus

Species name

Pineapple (Ananas comosus) is a species of monocot in the family Bromeliaceae (bromeliad family).

Species info

Gene sequence info

Ananas_comosus.5AT.fa

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>rna-XM_020227904.1
MVEILETSFVVPSEETPKKGLWLSNLDVAATRGHTPTVYFFRSNGDPDFFSAEAVKAALAKALVPFYPMAGRLGVDRDGRPEIDCNAEGAVFVLARSDQHTVDSFKDFEPSPEMRRLFVPAIDSADPPCPVLFLQNAGEWRWERRCTTWRWTGAAPSTSSKHGRRWPAGGRRRXLARGGEAAAGLSPPFHERTLLRERTPPAVLFDHPEYAPSAFPPRAPGPCVTTILKLSREQVAFLKARCVAPGGFVSTFRAVAAHVWRSMCVARGLAADAETRLFLPAEVRGHLRPPLPPHYFGNALVRVSAQARVGDIVAGPIGFAAERIKSAVDRVSDEYARSVIDHLGVIKRGGMSAKRGLPDTDLSVVSWLGMPMYEADFGWGAPQFMARAQTYGSGFVYLTSSPGKDAGISVVLGLEPDVMKQFKKLFYEEFCPLQT
>rna-XM_020230202.1
MKVKVESSKILKPLCEGYYPSTDHAYIPLSVFDTVTFDTHVAVLYAFRPPSPSNSAIQSGLARALAVYREWAGRLGADEDGNPVILLNDEGVRLVEASVDLPLCRAVPFKPSAALLSLHPGVDGVAELVQVQLTRFACGSLVVGFTAHHHVADGHATSNFLVAWGLASRGLAIPPRSPLRDRATLFAPRDPPRVEFEHRGVEFTTRKQLAVLKDENPSDGNPPDIAVHKAHFTREFLTSLKAKASLGAHRQFTTFECLVAHLWRAVTKARTLPNHQITFLRISVNGRMRIKPRVPKEFFGNCVLWAFPRAAAGELVSRPLRHAAGLIHEAVAGVDDAYFRSFVDFASSGGAKAEGLVPTAEAAKVVMCPNLELDSWLGFPFYDLDFGGGSPFYFMPTYSPMEGTLFLLPSFMGDGSVEAYVSLFDHSMASFKRICHSLDY
>rna-XM_020234431.1
MVEILESCMVVPSKETPQHKLWLSNLDVVAMRGHTPVLHVYRRTGVSPSFAVDTVKSALAEALAVYYPLAGKLAVDENGRIEADCTGEGALFVAAKSDEYAVDDVDGVVPTAEMRRLFVPAVESHELPCTVLAIQVTFLRCGGMILGTAIHHAVADGRCFFEFIKMMSDIARQVDGTQPASLSLDRSLLRALPTPKVTFDHPEYSPVSSDDDNNAPAAPFVVALFELSKDQLRFLKLRCSGDRNPPTAVSTFQAVAAHAWRCACIARGLLPSQETRLHTITDVHGRLKPPLPPRYLGNAAVRISVTTKVEKLISHSIRYGAEHIRRVLNNIGDEYVRSVVDYVEVTGISRLPASGKLLRTDLRIVSWLSLQSYAGDFGWGEPIYVARPHFSGGGHGYLVRSPRKDGGVGLTLALEVDSMERFKQLFYDEINEKAFSKCLRRNPRGSASSL
>rna-XM_020240174.1
MFPADFRRRVEPPIPAEYFGNCIGPCFAEADAEELLGGAGLAAAAAAIAGGIEELSSGLEGAENWMRRVKAVAAEQPLSAAGSPRFGVYETDFGWGRPRRVEIVSVANTGAVALAESPDEDEGGIQIGLGLRRPELESFQDYFVQALKDVTE
>rna-XM_020240175.1
MAPPPPPPPPPSPLMVKLRTIETCGVSPPEGSVPPSTVPLTFFDVFFLHARPMERLFFYPFPHPTSHFLSSFLPSLKSSLSLSLRSFFPLASLLRPSPDASDRFELFFSDATSPGVRFTVAECSDAQNAAGLFDHLADGRPRGRRPAPGASCLVVHARLRRSQPLLAVQVTVFPHHGVVVGTTMHHAACDGSSYTLFRQTWAAAARRWSGDISLDAHPLGPVVDRSLVPDPHGLYSLFYKTVAGKSPIPAPASSPADVVLASFTIKSSHIKALKAAFRCSTVAVTFAFTWINYVRARELAKRNDKVYLAIPVDFRSRMRPPLPAEYFGNCIGPCYVEAEAADLSGANGLTAAAEAIDRAIKSVASAGFEGAEPEKWLETIRGLPAEAILTGAGSHRFGVYDTDFGWGRPAHVDLNSVARTGVVAVAESGEEEGGVEIGLALPRNQMDLFQIYFAQGLGRLDG
>rna-XM_020242153.1
MFHFSREAVARLKARANEECGRGDLSSFQSLTALLWRAVTRARGLAPGQATSCRVAIENRARLRPPLAKEYFGNSIYVVAASATAGELLAQGIGWAAALVNRAVAEHTDTEIRAKLGAWMARPFVYTLREFDENSVMVGSSPRFDMYGCEFGWGRAVAARSGGANKFDGKISMYPGWEGGGSMDVELCLVPENMAALERDEEFMGAVSPPVEMEVLLEGIN
>rna-XM_020246041.1
MNVRVVSTKIIKPVHDHNSPQPIARSITSITLSAFDRVSEDMHIAAIYAFRPPAPSNSAVATGLARVLSHYPEWAGWLGHDENNNPAILLNDKGVKLTEASVDSTFDEAVPLDLSPVHLSLQPSKHGIEELLQVQLTRFACGALVVGYTMHHRVADGHAASQFLVAWGRACRGLEVHRHSSLDRCSLFSPRNPPHVDFEHRGVEFASEEFARCLDYSFKGKIITYKAHFTKEFLKKLKSQATSGAPPRRSYTTVESLVAHLWRAVTIARGLDKNDTTNVRISVNGRTRLRRNVPDDYFGNLILWAYPRTTVKELVSRPLHYVAELIHRAVADVDERYFKSFVDFASSGAVEAEGLVPAPEPKGRDMCRNLDVDSWIRFPLYELDFGGGSPFYFMPSYVPVEGLVNFLPSLVGDGRIEVHVSLFDHNVAAFQQLCYSLDADTRNYVHTHGYGVMSPGYNHNWDQVDKVKEMARL
>rna-XM_020245809.1
MAPSLPSSLDSSSGSAVAVVTVLSKCTVYPDAASVLGDLKLSVSDLPMLSCHYIQKGLFFSRPPIPIAALLSLLKTSLSRALSLVPALAGRLVTLPDGRIVISCDGSGVEFSHAAAPSLTLSSLLPPSSDVPLPVRSLFPCEGVISFDGHFRPLAAFQLTELGDGAVFVGCGVNHAVVDGTSLWSFFNAWAELCRGGAPEPPEFRRDFFGGSPAVLRFPGGAGPEVTFPVNAPLRERIFHFAREAVQDLKARANRRAVVRKLCSDRLREAEIFGKLAHDPKATSEGAGAGAGAGAGGGEAGAVTTFRMAVNCRHRVRPRVAAEYFGNAIQSVATRASVGDVASRELRWAAGLLHASVAAHGDAAVRKAVAEWEAAPRCFPLGNPDGAGITMGSSHRFPMYDGNDFGWGRPLAVRSGKANKFDGKISAFPGRDGDGSVDLEVCLAPDTMAALLQDHEFMSYASAHN
>rna-XM_020246751.1
MVASKAYTVTVTAKTTVAAALPMQEHRRPLSNLDLLLPPLDVGIFLCYENAVATSTTYATMVAALKASLARTLATYYPFAGEVVSNEAGEPELLCSNRGVDFVEARADAELRELCFHDPDESVEGKLVPKKKKGVLCVQATELKCGGVVVACTFDHRVADAYSTNMFIVSWAETVRSAPLSVLPTFRSSLLSPRPAAAVDASIDRLYAPLSSLPPPPDSDTAAAPVSVNRIYYIAAKDVTTLQAAAGKGKTKLEAFTACLWRLLAKAADADEKVCCMGVVVNGRGRLCGGEDDMLGYFGNVLSIPYGTLDVEALHGMKLDEVAEHVHGWVSAAATEKHFLNLVDWVESRRPVPSVARIYCGEEGGPACVVSSGRGFPEEADFGWGRPAFGSYHFPWGDGAGYVMPIPRGSGDWVVYAHLARRFVAALEEEPTVFRPLTTDYILNKIDSEK
>rna-XM_020250557.1
MANSCTITKKPPILVRACEATPRGSHRLSFLDRRPEIRLSLDVLLVFRRGGGEDPAKTIREGLSRALVHYYPVAGRIAEPAGGELAIECSGDGDGVWFVEASAEGRLDGFGDWDLILRLIFAAGLVARPPPEVNMENVIFMMQVTEFKCGGFTVGITYNHTVFDGAGSAQFLNAVAELTRGLAEPTVSPTWCRELIPSPPRPTTSAQGPEGPKIQFTFDSFKLAEFDISLQSVKQVKDRVTAETGQSCSTFDVVTAVVWQSRTRAINLDPDADVGLSFMANARHVLHELLSIEGGYYGNCVYTAGITLPSKQVANASLVVLVGLIREAKRTVSDGFSRWMNGDGATNLHALQDYRMLTVTDWNRLGYCEIDFGWGEPSFVYPCGCSSEFNVVCSCAFLKLPMPKQGIRLVMHCVEEQHSAALHDELMKFA
>rna-XM_020250915.1
MDESLEFEAEVVSMETIKPSTPTPPELRSYTLGWADQLARVYHLPVAFFYRGSPQTPAAERIRAMKSSLPRALSRFYPLAGELVLNTHVRCCDAGARFLEARADADLADFLRGLRGLHHVEKLLPCARSCSAAGAEGAPLLSVQATAFRCGGLALGVCACHKILDGRSLFLFLQSWASAAPDPVFDGAALFPPSEDVVPEEDQARPPGAPPLVRKGFVIAGPEVAALRSGLPSSTTRVQAVCALLWRCALRARPPPARASVASLLVDTRPRMAPPAPNASFGNRCFSAGAAAAKATVELVLARSGAARGVLEEELGRSAKMMNADHTRKLRAPDGHSSAYALIVDGVIRSRGFEGFDHYFFSSWLSLPLYEVDFGWGRPAWVGTTARVRNLSVLMPRSPEVDPRGVEVWVTMEESEMAEFEKDEELLQFAAPFRVVTPPPGRA
>rna-XM_020251118.1
MLQCDKKFSRFELYIYIYIIYINLYGGGGKGRGVPKLNLACCCNFLNLDCVTSCAMWSVRVLSEETVKPSSRTPPDLRTYKLSWLDQVTYIHHIPVTFFFLFPPSDAVQTLRSSLSNTLSRFYPLAGRQIGDTHVDCADQGVDFFEARVEARLAELLARPTPDLDFMFDLLPCSTAGCLTRADGRGSLLKVKATAFACGGLAVAACLSHADARSLFAFFRAWAAEASGSGAAPAPDFRAAARLPPNESAAPMKAGEEGPHAPRNARKRFLLDGSKILAAARRDSPASERAEAVGALLWRCALRARARLGAPARASVCSPQVDLRGRLDPPAPHHAFGNHCVMSGAAATAEEVSKVVGGDASASGALEEELRRAEKKVGGEFVRRLQGPEGHAAAYQLVGEAVRGSGGAATGFDLYFLSDWLDLPAYDVDFGWGTPAWVGYSRTKAKNLMLLMPAAPSVGDPTAVEAWVALEEKELEEFERDEELASFLVAASSEPLPVREA
>rna-XM_020251690.1
HGIRVRGGGDRGVPVAQDRRRAVDGVVPSLLGGRGDEPDPLFDAADAFPPSDDAVPPTEPEAGPGEKKPQVVKRFPLDPRKIAALRPAPAPAPAPAPTRVQAVTALLWRGLIRARARAARGGAPPKASVASLIVDTRARTDPPAPDHAFGNRCVLAAAALAAETAAGVAVGEGEGCGGAALEEEIGSSVKKVGGEHAKRLQGPSGHTSAFALVGEAVRGCGGFRGFDLHVISSWLRLPVYEVDFGWGKPAWVGINRNVSRDGAVLLPAPFHAMDGRGVEAWVSLSEDEMAEFEKDAELTSCLLAVNS
>rna-XM_020254469.1
MVVVEVIKSELVAPSEATPKDPIWSSNLDLAARRGYTPTVYFYRPNGDSDFFSEDVLKTALAKALVRFYPVDGRVEISCTAEGALFVVARADAALDDFNDFAPSTAMRDLFVPPACSPDPPCILLMLQVTYLKCGGVVLGTAMHHTVIDARSAFHFISTWSAIARGGGGGADLPPPPFLDHTLLRARSPPVVLFDHPEYKPTPNPNPNPNPNPNSSPNPNRSSTPAPYASAILRLSQAQVDALKARCCGGGGGGGARVSAFRAIVAHVWRCVCAARGLARGAETRLYTMVDVRARLAPPLPPNYFGNAVLRTSVAAAAGELADAPLGSVARRLRAATSPGDDYARSLVDYLDAVDMDRLPRSGLPGNDLRVISWMGMSIYDADFGWGEPVFMGPALMYYAGFVYLMSSPGKVGGVSVAVSLEPDTLPTFKKLLFQELGGVQG
>rna-XM_020256598.1
MDQNHPLYHLPVVFFYSATPSAADDIGTLKSSLSTVLASFYPLAGRLSGNSLVDCGDQGAEFFEARAPNADLSEILSSDEPDLGEVKKFLPCSKGEASCCDSEPTVILSVQATVFRCGGIAVGVCISHKIADGRSFFSFLQGWAAAARGSFSSEYSPSFDGAAVFPPKENAVPNAAEQPPEKPPQVHKRFVLGGPKIAALREKTASSAATRFQAVAALVWRCAVRARPVARVAVAVVVANTRSRMELRSPNGSFGNCCTGTLAGIPMDEVENIAKSDVTENTKCVLEEEMGAAVKRVDAEYVRRLQGEDGWQRQLELGWEAVRKCGGATEAGFDMNVFSSWLRLPVYEVNFGWGPAAWACMTQLRPSSIVVLPRPPAARDPQGMEVWASLEQVEMAELQRELEKLVPLNPVHHVTQR
>rna-XM_020227058.1
MHHTVIDARSAFHFISTWSAIARGGGGADLPPPPFLDHTLLRARSPPVVLFDHPEYKPTPNPNPNPNPNSSPNPNPSSTPAPYASAILRLSQAQVDALKARCCCGGGGGGGARVSAFRAIVAHVWRCVCAARGLARGAETRLYTMVDVRARLAPPLPPNYFGNAVLRTSVAAAAGELADAPLGSVARRLRAATSPGDDYARSLVDYLDAVDMDRLPRSGLPGNDLRVISWMGMSIYDADFGWGEPVFMGPALMYYAGFVYLMTSPGKVGGVSVAVSLEPDTLPTFKKLLFQELGGVQG

Gene tree

Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University