Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Nyssa sinensis
Species name
Nyssa sinensis is a species of eudicot in the family Nyssaceae.
Species info
Gene sequence info
Nyssa_sinensis.COMT.fa
Download
>Nsi03305.mRNA1
MGSTENHVSPSIKEDDEACAYALQFCLSHVPHMVMNAAVELELFDIIAQKGSGAHISSSEIVSQLPAQNPDAASILDRMLCLLASYSLLTCSLRTHQDGTVERLYGLAPAGKFFVRNQGGRSLASMFPFTCYPPIWTTWFHLKDAILEGGSILKKACGMSVFQFMDADPTFNSLFNKSMADMSNITMLKLLETYKGFEELGSLVDAGGGTGTSLNMIISKYPSIKGINFDLPHVIQSAPSYPGIEHVGGDMFVNIPAAEAIMVKNVIHNWNDEHCLKLLRNCYEALPKKGKVIIIDFVMPLTPEQTSAAKFAALMDVTMLNTPNGKERTEKEFEALCKGAQFSDFKIICTILSLGVIEFRK
>Nsi03306.mRNA1
MALHEMLVQLITLLIAILSARAIAATSQPKPGCPTNCGNLSIPFPFGTSDGCYLDDSFFLTCNDIQYNPPRTFAGDEQVLDISVNGHMRVASNFAQDCFNKSGLLFKKSTNFSLPNFVISSTRNKFTAVGCDTIGVAAGVGSEGQNYAIGCVSFCNLFESVDNGSCSGFGCCQTSMPQGATEFAVVVGSIWNHANISDFNPCGYAFLVEEDYFNFSNLDLKDFQNRENVPMVLDWAIGNETCEEAKKNSSSYACRAVNSDCYNSPNGLGYFCNCSGGYQGNPYLIDDCQDIDECEISNPCVKKCKNLPGNFSCPCPEGYDGDGRKDGTGCQYKGSILLNVSLGLSISLLVLVVGSSWIYWIVKKRKHMKLKEKFFLQNGGLMFQQQLSRHEGSIETAKIFTIEDLKKATKNYDESKVIGRGGFGTVYKGVLPDNTVVAIKKSKISDQNQIEQFINEVIVLSQINHRNVVKLLGCCLETEVPLLVYEFITNGTLSYHIHNEGRATSLSWEMRLKIAAETAGAIAYLHSAASTPIIHRDIKSTNILLDDNYTAKVADFGASKLVPVDQAQLTTLVQGTFGYLDPEYFHSSQLTEKSDVYSFGVLLAELLTGEMALSFVRPEKDRNLAMYFDSLMEEGRLFQILDHRPVNEGKFEQLKEVAELARRCLRVKREERPTMKEVAMELEGLRVMEKHPWVNVNLYPEETELLLAKTIPSDGYNNGEENRAQLKVEAKKASILNKHELNRKPVELELFDIIAQKGSGAHISSSEIVSQLPAQNPDAASILDRMLCLLASYSLLTCSLRTHQDGTVERLYGLAPAGKFFVRNQSGRSLASMFPFAYHPPIWTAWFHLKDAILEGDNIFKKACGMSVFQFMNADPTFNSLFNKSMADMSNITMLKLLETYKGFEGLGSLVDVGGGIGTSLNMIISKYPFIKGINYDLPHVIQSAPSYPGIEHVGGDMFVSIPKGEAIMVKNVFHNWNDERCLKLLHNCYEALPKKGKVIIIDFVMPLIPEQSTSAKVAALMDITMLNTPSGKERTVKEFEALCKGARFSDFNSISSAHAFGVIEFLK
>Nsi01534.mRNA1
MGASSGPKLSGMQKQVLSLYRGFFRAARSKSPEDRRQIESLVSAEFHRNSNLVDRKNFLYIEYLLRRVSMDTRLVTKIVIRINIIMLAHKPGRKERTEESEALAKRD
>Nsi13993.mRNA1
MSSEEKEIRLSSEEEDDINCQYAMQLACSSVLPMALKAAIELGLLDIIERAGPGALLSPTEIASQLPTSNPDAPVLLDRILRLLASHSVLTCSLGDGEQHEIQRLYGLAPVCKYFIRNQDGVSLAPFFEIIQSKVMIDMWYHMKDAVVEGGLPFNRAYGMSSSEYVGKDAKLLQLFKASMKEYNSMFMRMILEKYKGFQDLKSMVDVGGGDASILNMIVSKYPTIKAINFDLPSIIDKSPTYPGIEHVAGDMFVSIPKGDAIFMKWILHSWDDEHCLKILKICYEALPEHGKVIIVDLVVPEVPETSIPVKSVLQLDLFLMNMNPGGKERTEKEFEILAKAAGFSSIRVAYCAYGFSLVEFYKNM
>Nsi30853.mRNA2
MESSARTCYYHEDGGEEEQCSNAMQLVNSSVLPMVMQAAVELDLLEFIAKAGPGAQLSPSEIASQMPTTIPGAPAMLDRILRLLASYSVLTCSVVDADDGHPRRLYGLAPVAKYFVRNEDGVSLGPLMALLQDKVFMDSWYALKDAVLEGGIPFNKVHGTHAFDYPARDPRFNEVFNKAMINHTTIVIKRILESYEGFEHLKQLVDVGGGLGVTLNMITSKYPTIRGINFDLPHVIQHAPHYPGVEHVGGDMFASVPKGDVHFYEGKFEFCRYP
>Nsi16391.mRNA1
MENKAGLDYLSVWGLGGLIVTQMAIKAAIELNVFNIISDSGAEAQLSSAEIVSKIPTTNPNAATVLDRILRVLGSSSLLSTSVRPCPNGEERTYGLSMESRALVTNTDGVCLAPVMLMVSQREVVESLYLLKNMVLEPEYVPFQKAHGVNFFEYAAKDPKMGRMFDDSMACHSKIAFEMVLKVYTGFEQVKELMDVGGGNGTLLGRIVSTYPNVRGINFDLPHVIAEAPSFPGVEHVAGDMFEPLPDAQTILLKSVLHDWDDDNCIKLLRNCWKALPANGMVIVIESAIPQVSDNDAELQTVILMDVAIMVVAGGKERSAAEFDNLAKAAGFAETRIFHIAQGIHVMEFIKKITE
>Nsi29350.mRNA1
MENKAGLDYLSVWGLGGLIATQMAIKAAIELNVFNIISDSGAEAQLSSAEIVSKIPTTNPNAAAVLDRILRVLGSRSLLSTSSRPCPNGEERTYGLSMESRALVTNTDGVCLAPVMLMASQRELVESLYLLKNMVIEPGYLPFQKAHGVNFFEYAAKDPKMGRMFDQSMACHSKMAFEMVFKVYTGFKQVKELMDVGGGNGTLLGRIVSTYPNVRGINFDLPHVIADAPNFPGVEHVAGDMFEPLPDAQTILLKSVLHDWDDDNCVKLLRNCWKALPANGMVIVIESAIPPVSDNDAELQTVILMDVAMMVVAGGKERSTAEFDNLAKAAGFAETRIFHIAQGIHVMEFVKKITE
>Nsi01251.mRNA1
MGSTSEVHTSPAEAEEEACLFAIQLASASVLPMVMKSAIELDLLEIMAKAGPGAYISPSELAAQLPTKNPDAPVMLDRILRLLASYSVLNCTLRNLPDGGVERLYGLAPVCKFLTRNADGVSMAPLLLMNQDKVLMESWYYLKDAVLNGGIPFNKAYGMTAFEYHGTDPRFNKVFNQGMSNHSTITMKKILETYKGFDGLGSVVDVGGGTGATLNMIVSKHPTIKGINFDLPHVIEDATSYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYKALPDNGKVIVAECILPVSPDARLATKNVIHIDVIMLAHNPGGKERTEKEFEALAKGAGFQGFRVMCSAYNTWVMEFLKKI
>Nsi22263.mRNA1
MVLNAAIELHLFDIIARAGPHTQISAADFASKLPTQNPDAPAMLDRILRLLASHSLLSCSLHTLEDGTVERRYGLLPAGKFFAQNQDGPSLASVVSLANHRAPAEVRSNLKDAILEGGNQFKKVHGMSIFEYMNIDQTLNNVFNKAMSDISSLVMKKILEAYKGFEGLESLVDVGGGIGMSLNMIISMYPSIKGINFDLPHVIQTAPSYPGIVHVGGDMLVSIPKGDAIMIKNACHNWSDDHCVKILNNCCEALPDNGKTIVVDIVMPMAPESSNAAKYGTQLDNLMLLEPGGKERTQRDFEALCKASKFSSFQIVCCAYAFSVIEFYK
>Nsi24721.mRNA1
MGSTSEIQTSSAEAAEEEACLFAMQLASASVLPMVLKSAIELDLLETMAKSGPATPFSTVHFETSPTGHVERLYGLAPVCKFLTRNADGVSMAPLLLMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNQGMSNHSTITMKKILETYNGFDGLASVVDVGGGTGATLNMILSKYPAIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSVPKADAIFMKWICHDWSDEALPEIFEELLRSTSRQWKSDCCRMHSSSSPRFQSCH
MGSTENHVSPSIKEDDEACAYALQFCLSHVPHMVMNAAVELELFDIIAQKGSGAHISSSEIVSQLPAQNPDAASILDRMLCLLASYSLLTCSLRTHQDGTVERLYGLAPAGKFFVRNQGGRSLASMFPFTCYPPIWTTWFHLKDAILEGGSILKKACGMSVFQFMDADPTFNSLFNKSMADMSNITMLKLLETYKGFEELGSLVDAGGGTGTSLNMIISKYPSIKGINFDLPHVIQSAPSYPGIEHVGGDMFVNIPAAEAIMVKNVIHNWNDEHCLKLLRNCYEALPKKGKVIIIDFVMPLTPEQTSAAKFAALMDVTMLNTPNGKERTEKEFEALCKGAQFSDFKIICTILSLGVIEFRK
>Nsi03306.mRNA1
MALHEMLVQLITLLIAILSARAIAATSQPKPGCPTNCGNLSIPFPFGTSDGCYLDDSFFLTCNDIQYNPPRTFAGDEQVLDISVNGHMRVASNFAQDCFNKSGLLFKKSTNFSLPNFVISSTRNKFTAVGCDTIGVAAGVGSEGQNYAIGCVSFCNLFESVDNGSCSGFGCCQTSMPQGATEFAVVVGSIWNHANISDFNPCGYAFLVEEDYFNFSNLDLKDFQNRENVPMVLDWAIGNETCEEAKKNSSSYACRAVNSDCYNSPNGLGYFCNCSGGYQGNPYLIDDCQDIDECEISNPCVKKCKNLPGNFSCPCPEGYDGDGRKDGTGCQYKGSILLNVSLGLSISLLVLVVGSSWIYWIVKKRKHMKLKEKFFLQNGGLMFQQQLSRHEGSIETAKIFTIEDLKKATKNYDESKVIGRGGFGTVYKGVLPDNTVVAIKKSKISDQNQIEQFINEVIVLSQINHRNVVKLLGCCLETEVPLLVYEFITNGTLSYHIHNEGRATSLSWEMRLKIAAETAGAIAYLHSAASTPIIHRDIKSTNILLDDNYTAKVADFGASKLVPVDQAQLTTLVQGTFGYLDPEYFHSSQLTEKSDVYSFGVLLAELLTGEMALSFVRPEKDRNLAMYFDSLMEEGRLFQILDHRPVNEGKFEQLKEVAELARRCLRVKREERPTMKEVAMELEGLRVMEKHPWVNVNLYPEETELLLAKTIPSDGYNNGEENRAQLKVEAKKASILNKHELNRKPVELELFDIIAQKGSGAHISSSEIVSQLPAQNPDAASILDRMLCLLASYSLLTCSLRTHQDGTVERLYGLAPAGKFFVRNQSGRSLASMFPFAYHPPIWTAWFHLKDAILEGDNIFKKACGMSVFQFMNADPTFNSLFNKSMADMSNITMLKLLETYKGFEGLGSLVDVGGGIGTSLNMIISKYPFIKGINYDLPHVIQSAPSYPGIEHVGGDMFVSIPKGEAIMVKNVFHNWNDERCLKLLHNCYEALPKKGKVIIIDFVMPLIPEQSTSAKVAALMDITMLNTPSGKERTVKEFEALCKGARFSDFNSISSAHAFGVIEFLK
>Nsi01534.mRNA1
MGASSGPKLSGMQKQVLSLYRGFFRAARSKSPEDRRQIESLVSAEFHRNSNLVDRKNFLYIEYLLRRVSMDTRLVTKIVIRINIIMLAHKPGRKERTEESEALAKRD
>Nsi13993.mRNA1
MSSEEKEIRLSSEEEDDINCQYAMQLACSSVLPMALKAAIELGLLDIIERAGPGALLSPTEIASQLPTSNPDAPVLLDRILRLLASHSVLTCSLGDGEQHEIQRLYGLAPVCKYFIRNQDGVSLAPFFEIIQSKVMIDMWYHMKDAVVEGGLPFNRAYGMSSSEYVGKDAKLLQLFKASMKEYNSMFMRMILEKYKGFQDLKSMVDVGGGDASILNMIVSKYPTIKAINFDLPSIIDKSPTYPGIEHVAGDMFVSIPKGDAIFMKWILHSWDDEHCLKILKICYEALPEHGKVIIVDLVVPEVPETSIPVKSVLQLDLFLMNMNPGGKERTEKEFEILAKAAGFSSIRVAYCAYGFSLVEFYKNM
>Nsi30853.mRNA2
MESSARTCYYHEDGGEEEQCSNAMQLVNSSVLPMVMQAAVELDLLEFIAKAGPGAQLSPSEIASQMPTTIPGAPAMLDRILRLLASYSVLTCSVVDADDGHPRRLYGLAPVAKYFVRNEDGVSLGPLMALLQDKVFMDSWYALKDAVLEGGIPFNKVHGTHAFDYPARDPRFNEVFNKAMINHTTIVIKRILESYEGFEHLKQLVDVGGGLGVTLNMITSKYPTIRGINFDLPHVIQHAPHYPGVEHVGGDMFASVPKGDVHFYEGKFEFCRYP
>Nsi16391.mRNA1
MENKAGLDYLSVWGLGGLIVTQMAIKAAIELNVFNIISDSGAEAQLSSAEIVSKIPTTNPNAATVLDRILRVLGSSSLLSTSVRPCPNGEERTYGLSMESRALVTNTDGVCLAPVMLMVSQREVVESLYLLKNMVLEPEYVPFQKAHGVNFFEYAAKDPKMGRMFDDSMACHSKIAFEMVLKVYTGFEQVKELMDVGGGNGTLLGRIVSTYPNVRGINFDLPHVIAEAPSFPGVEHVAGDMFEPLPDAQTILLKSVLHDWDDDNCIKLLRNCWKALPANGMVIVIESAIPQVSDNDAELQTVILMDVAIMVVAGGKERSAAEFDNLAKAAGFAETRIFHIAQGIHVMEFIKKITE
>Nsi29350.mRNA1
MENKAGLDYLSVWGLGGLIATQMAIKAAIELNVFNIISDSGAEAQLSSAEIVSKIPTTNPNAAAVLDRILRVLGSRSLLSTSSRPCPNGEERTYGLSMESRALVTNTDGVCLAPVMLMASQRELVESLYLLKNMVIEPGYLPFQKAHGVNFFEYAAKDPKMGRMFDQSMACHSKMAFEMVFKVYTGFKQVKELMDVGGGNGTLLGRIVSTYPNVRGINFDLPHVIADAPNFPGVEHVAGDMFEPLPDAQTILLKSVLHDWDDDNCVKLLRNCWKALPANGMVIVIESAIPPVSDNDAELQTVILMDVAMMVVAGGKERSTAEFDNLAKAAGFAETRIFHIAQGIHVMEFVKKITE
>Nsi01251.mRNA1
MGSTSEVHTSPAEAEEEACLFAIQLASASVLPMVMKSAIELDLLEIMAKAGPGAYISPSELAAQLPTKNPDAPVMLDRILRLLASYSVLNCTLRNLPDGGVERLYGLAPVCKFLTRNADGVSMAPLLLMNQDKVLMESWYYLKDAVLNGGIPFNKAYGMTAFEYHGTDPRFNKVFNQGMSNHSTITMKKILETYKGFDGLGSVVDVGGGTGATLNMIVSKHPTIKGINFDLPHVIEDATSYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYKALPDNGKVIVAECILPVSPDARLATKNVIHIDVIMLAHNPGGKERTEKEFEALAKGAGFQGFRVMCSAYNTWVMEFLKKI
>Nsi22263.mRNA1
MVLNAAIELHLFDIIARAGPHTQISAADFASKLPTQNPDAPAMLDRILRLLASHSLLSCSLHTLEDGTVERRYGLLPAGKFFAQNQDGPSLASVVSLANHRAPAEVRSNLKDAILEGGNQFKKVHGMSIFEYMNIDQTLNNVFNKAMSDISSLVMKKILEAYKGFEGLESLVDVGGGIGMSLNMIISMYPSIKGINFDLPHVIQTAPSYPGIVHVGGDMLVSIPKGDAIMIKNACHNWSDDHCVKILNNCCEALPDNGKTIVVDIVMPMAPESSNAAKYGTQLDNLMLLEPGGKERTQRDFEALCKASKFSSFQIVCCAYAFSVIEFYK
>Nsi24721.mRNA1
MGSTSEIQTSSAEAAEEEACLFAMQLASASVLPMVLKSAIELDLLETMAKSGPATPFSTVHFETSPTGHVERLYGLAPVCKFLTRNADGVSMAPLLLMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNQGMSNHSTITMKKILETYNGFDGLASVVDVGGGTGATLNMILSKYPAIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSVPKADAIFMKWICHDWSDEALPEIFEELLRSTSRQWKSDCCRMHSSSSPRFQSCH
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University