Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Nyssa sinensis
Species name
Nyssa sinensis is a species of eudicot in the family Nyssaceae.
Species info
Gene sequence info
Nyssa_sinensis.4CL.fa
Download
>Nsi36068.mRNA1
MQAKVVDWICGSFLPPGCSGELWLRGPAVMKGYLNNMEATTITIDRDGWLHTGDIVYFDEDGYLHIYDRLKEIIKYKGFQIAPADLEAVLISHPDILDAAVTAAMDEEAGEIPVAFVVKRQGSVLSQAAVVDFVAKQVAPYKKVRKVAEFYSSTQLQISKAFKEKSIAFIHIIGNYVKDVQAQIYYRDVLVSSSQLLSRMIP
>Nsi18414.mRNA1
MAAETLFGDNGIYTSPRPPLSLPTDPNLSMLPFLFRNLSSFHNHPALIDGDTSETLTFSELKTVVSKLAHSLLKLNIKNNDVVLIFAPNSIHFVVSFLAVVAISAIATTVNPQYTITELSNQVKDSNPKLIITLHELYHNVKQLNLPSIILGHQNENHGIANNTKIWYYTDLIKFSDNATFEFPAIVQTQIATILYSSGTTGTSKGVILTHRNFIATALMVASDQDVYGDKRNVFLCFLPMFHIFGLSVVTFAQLQRGHTVVSMARFEMGKVLGAIEKYRVTHLFVVPPVVIALTKHSAVLKKYDLSSLKEIGSGAAPLGEDVMEDCAKNFPQAAIFQGYGMTETCGIISLENTMIGSRHSGSTGVLVPGVESQIVSIDTLKPLPPNQLGEICVRGPNMMQGYFNNPQATELTIDKQGWVHTGDLGYFDEDGQLYVVDRIKELIKYKGFPVAPAELEGLLLSHPEILDAVVIPYPDAEAGEVPIAYAVRSPSSSLTEEEAQNYIAKQVAPFKRLRKVNFTNSVPKSASGKILRRELTQKVRAKL
>Nsi35190.mRNA1
MEKSGYGRDGIFRSLRPPLVLPRDPNLSMVSFLFPKNSSFPDKPALVDADTGETLTFAQFKSTVCKVSHGLLQLGIEKNDVVLFFAPNSIQFPLCFFGIIALGAIATTVNPTYTVSELSKQIKDCNPKLLITVPQLWEKVKVFGLPAVILGSERSSKFMDSSSRTTLFTDLVNNSRLVSNFPEASIKQSDTAALLYSSGTTGTSKGVVLSHRNFIAAALMVTADQGLAGEMYNVFLCVLPMFHAFGLCVILYSQLQRGNAVVSMGKFDLEMLLMAVEKYRVTHLWVVPPIILALSKQSVVKKYDLSSLKQIGSGAAPLGKELMDECAKNFPYAVILQGYGMTETCGTVSYENQYVGSRHSGSAGTLAPGVESQIVTIDTLKPLPPNKLGEIWIRGPNMMQGYFKNPQATKLTIDKQGWVHTGDLGYFDEEGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHREILDAVVIPFPDAEAGEVPIAYVVRSPNSSLSEEDVQKFIADQVAPFKRLRKVTFVNSVPKSASGKILRRELIEKVRSKL
>Nsi35189.mRNA1
MVSFLFRNTSSFPDKPALFDADTGETLTFAQFKSTVSKVSHGLLQLGIKQNDVVLIFAPNSIQFPLCFFGIIAIGAIATTVNPIYTVSELSKQIKDCNPKLIITVPQLWEKVKVFGLPAVILGSEKSWKLMDSTSRIISFTDLVNNARPVSNFGEASIKQSDTAALLYSSGTTGTSKGVVLSHRNFIAAALMVTADQELSGEMYNVFLCVLPMFHVFGLCVIMYSQLQRGNAIVSMGKFDLEMILRSVEKYRVTHLWVVPPIILALSKQSVVKKYDLSSLRQIGSGAAPLGKELMEECAKNFPHAVVLQGYGMTETCGIVSVENQYAGSRHSGSTGTLVPGVESQIVSVDTLKPLPSNKLGEIWVRGPNMMQGYFNNPQATKLTIDKQGWVHTGDLGYFDEEGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHREILDAVVIPFPDAEAGEVPIAYVVRSPNSSLTEEDVQKFIADQVAPFKRLRKVTFVNSVPKSASGKILRRELIEKVRSKL
>Nsi35188.mRNA1
MENYGYGRDGIFRSLRPPRVLPRDPNLSMVSFLFRNTSSFPDKPALIDADTGETLTFAQFKSTVSKVSHGLLQLGIKKNDVVLIFAPNSIQFPLCFFGIIAIGAVASTVNPAYTVSELSKQIKDCNPKLIITVPQLWEKAKVFGLPAVILGSETSSKFMDSSSRITLFTDVVNNSRLMSNFPESSIKQSDTAALLYSSGTTGTSKGVVLSHRNFIAAAIMVTADQELAGEMYNVFLCVLPMFHVFGLCVIMYSQLQRGSAVVSMGKFDLEMILRVIEKYRVTNLWAVPPIILALSKQSVVKKYDLSSLRQIGSGAAPLGKELMEECAKNFPHAVVFQGYGMTETCGVISIENQNVGSRHSGSTGPLVPGVESQIVSVDTLKPLPPSKLGEIWVRGPNMMQGYFNNPQATELTIDKQGWVHTGDLGYFDEKGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHHEILDAVVIPFPDAEAGEVPIAYVVRSPNSLLTEEDVQKFIADQVAPFKRLRRVTFVKSIPKSPSGKILRRVLIEKVRSKL
>Nsi10884.mRNA1
MDQTQTKQKEIIFRSKLPDLYIPKHLPLHSYCFENISKFSSRPCLINATGEVHTYAEVELTARKVASGLNKLGVKQGETIMILLQNSPEFIFAFLGASYRGANSTMANPFFTPAEVVKQAKASNAKIIITQASHVEKVKDYATQNDVKVMCIDSPPEGCLHFSELTQADENEMPEVEISPDDVVALPYSSGTTGLPKGVMLTHRGQVTSVAQQVDGENPNLYMHSEDVMLCVLPLFHVYSLNSVLFCGLRVGAALLIMQKFEIVTVLELIQKYKVTIAPVVPPIVLAIAKSPAVDNYDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPDTGASLPRNQPGEICIRGDQVMKGYLNDPEATERTIDKQGWLHTGDIGYIDDDEELFIVDRLKELIKYKGFQVAPAELEAMLLNHPKISDAAVVPMKDDAAGEVPVAFVVRSKGSDVTEDEIKQYISKQVVFYKRLNRVFFIDAIPKAHSGKILRKDLRAKLAAGFPN
>Nsi31379.mRNA1
METKREPEEFIFRSKLPDIYIPNHLPLHTYCFENISQFSSRPCVINGATGDIYSYADVELTARKVAAGLDKLGIQQGDVIMLLLQNSPEFVFAFLGASYRGATSTTANPFYTPAEIAKQAIASKAKLIITQSCYAEKADEKDMPAVKINPDDAVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMQKFEINALMELVHKYKVTIAPFVPPIVLAIAKSPVVDKYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGSCGTVVRNAEMKIVDPDTGASLTRNQAGEICYLNDPEATERTIDKEGWLHTGFQVAPCGARSIVDSPIPTSPMLLLYP
>Nsi24220.mRNA1
MINLNPNPNPNPFHLSVMAQDEEHIFRSLYPSVPVPNDVTLPDFVLQDAELYASKVAFVEAVTGKEYTYGEVVRDIRRFAKALRSLGLRKGKVVVVVLPNVAEYAIVALGIMMAGGVFSGANPSAHASEIKKQVEVADAKLIVTNRSTYEKVKALELPVIILGEEHVEGTMNWDGLLEAADKASTDITNIETIHQTDLCALPFSSGTTGVSKGVMLTHHNLVANLCSSLFSVGPELVGQVTILGLMPFFHIYGMTGICCATLRNKGKVVTMQRYEIEPFLNALIAHEVTFAPIVPPIILELVKNPIVDEFYVRKLKLRSIMTAAAPLAPEILNAFEHKFPGVQVQEAYGMTEHSCITLTHGNPSKGHRNAKRNSVGFILPNMELKFIDSETGRSLPNNSPGEICVRSQCVMKGYYKNEEETAHTIDKNGWLHTGDVGFIDDDGDVFIVDRIKELIKYKGFQVAPAELEGILLTHPSIEDAAVLGLPDEEAGEIPAACVVMNPNAKESEMEIMSYVASNVANYKRVRMLQFVDKIPTSPSGKIMRRLLKEEMLKKMKNKPKAIPTLQSN
>Nsi37344.mRNA1
MEAKQDQQEFIFRSKLPDIYIPNHLPLHTYCFENISQFSSRPCIINGPTGDIYTYADVELTSRKVAAGLDKLGIQQGDVIMLLLQNSPEFVFAFLGASYRGATSTTANPFYTPAEIAKQVKASKAKLIITQSCYTEKVMEFGKENDVKIMCIDSPLEGCLHFSELTQADEKDIPAVKIHPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHRDDVIMCVLPLFHIYSLNSVLLCALRVGAAILIMQKFEINTLLELVQKYKVTIAPFVPPIVLAIAKSPVVDKYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKPGSCGTVVRNAEMKIVDPDTAASLPRNQAGEICIRGNQIMKGYLNDPEATENTIDKERWLHTGDIGFIDDNDELFIVDRLKELIKYKGFQVAPAELEAMLISHPNISDAAVVPMKDDTAGEVPVAFVVKAIGSKITEDEIKQYISKQVVFYKRINRVFFTETIPKAPSGKILRKDLRAKLATGLPN
MQAKVVDWICGSFLPPGCSGELWLRGPAVMKGYLNNMEATTITIDRDGWLHTGDIVYFDEDGYLHIYDRLKEIIKYKGFQIAPADLEAVLISHPDILDAAVTAAMDEEAGEIPVAFVVKRQGSVLSQAAVVDFVAKQVAPYKKVRKVAEFYSSTQLQISKAFKEKSIAFIHIIGNYVKDVQAQIYYRDVLVSSSQLLSRMIP
>Nsi18414.mRNA1
MAAETLFGDNGIYTSPRPPLSLPTDPNLSMLPFLFRNLSSFHNHPALIDGDTSETLTFSELKTVVSKLAHSLLKLNIKNNDVVLIFAPNSIHFVVSFLAVVAISAIATTVNPQYTITELSNQVKDSNPKLIITLHELYHNVKQLNLPSIILGHQNENHGIANNTKIWYYTDLIKFSDNATFEFPAIVQTQIATILYSSGTTGTSKGVILTHRNFIATALMVASDQDVYGDKRNVFLCFLPMFHIFGLSVVTFAQLQRGHTVVSMARFEMGKVLGAIEKYRVTHLFVVPPVVIALTKHSAVLKKYDLSSLKEIGSGAAPLGEDVMEDCAKNFPQAAIFQGYGMTETCGIISLENTMIGSRHSGSTGVLVPGVESQIVSIDTLKPLPPNQLGEICVRGPNMMQGYFNNPQATELTIDKQGWVHTGDLGYFDEDGQLYVVDRIKELIKYKGFPVAPAELEGLLLSHPEILDAVVIPYPDAEAGEVPIAYAVRSPSSSLTEEEAQNYIAKQVAPFKRLRKVNFTNSVPKSASGKILRRELTQKVRAKL
>Nsi35190.mRNA1
MEKSGYGRDGIFRSLRPPLVLPRDPNLSMVSFLFPKNSSFPDKPALVDADTGETLTFAQFKSTVCKVSHGLLQLGIEKNDVVLFFAPNSIQFPLCFFGIIALGAIATTVNPTYTVSELSKQIKDCNPKLLITVPQLWEKVKVFGLPAVILGSERSSKFMDSSSRTTLFTDLVNNSRLVSNFPEASIKQSDTAALLYSSGTTGTSKGVVLSHRNFIAAALMVTADQGLAGEMYNVFLCVLPMFHAFGLCVILYSQLQRGNAVVSMGKFDLEMLLMAVEKYRVTHLWVVPPIILALSKQSVVKKYDLSSLKQIGSGAAPLGKELMDECAKNFPYAVILQGYGMTETCGTVSYENQYVGSRHSGSAGTLAPGVESQIVTIDTLKPLPPNKLGEIWIRGPNMMQGYFKNPQATKLTIDKQGWVHTGDLGYFDEEGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHREILDAVVIPFPDAEAGEVPIAYVVRSPNSSLSEEDVQKFIADQVAPFKRLRKVTFVNSVPKSASGKILRRELIEKVRSKL
>Nsi35189.mRNA1
MVSFLFRNTSSFPDKPALFDADTGETLTFAQFKSTVSKVSHGLLQLGIKQNDVVLIFAPNSIQFPLCFFGIIAIGAIATTVNPIYTVSELSKQIKDCNPKLIITVPQLWEKVKVFGLPAVILGSEKSWKLMDSTSRIISFTDLVNNARPVSNFGEASIKQSDTAALLYSSGTTGTSKGVVLSHRNFIAAALMVTADQELSGEMYNVFLCVLPMFHVFGLCVIMYSQLQRGNAIVSMGKFDLEMILRSVEKYRVTHLWVVPPIILALSKQSVVKKYDLSSLRQIGSGAAPLGKELMEECAKNFPHAVVLQGYGMTETCGIVSVENQYAGSRHSGSTGTLVPGVESQIVSVDTLKPLPSNKLGEIWVRGPNMMQGYFNNPQATKLTIDKQGWVHTGDLGYFDEEGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHREILDAVVIPFPDAEAGEVPIAYVVRSPNSSLTEEDVQKFIADQVAPFKRLRKVTFVNSVPKSASGKILRRELIEKVRSKL
>Nsi35188.mRNA1
MENYGYGRDGIFRSLRPPRVLPRDPNLSMVSFLFRNTSSFPDKPALIDADTGETLTFAQFKSTVSKVSHGLLQLGIKKNDVVLIFAPNSIQFPLCFFGIIAIGAVASTVNPAYTVSELSKQIKDCNPKLIITVPQLWEKAKVFGLPAVILGSETSSKFMDSSSRITLFTDVVNNSRLMSNFPESSIKQSDTAALLYSSGTTGTSKGVVLSHRNFIAAAIMVTADQELAGEMYNVFLCVLPMFHVFGLCVIMYSQLQRGSAVVSMGKFDLEMILRVIEKYRVTNLWAVPPIILALSKQSVVKKYDLSSLRQIGSGAAPLGKELMEECAKNFPHAVVFQGYGMTETCGVISIENQNVGSRHSGSTGPLVPGVESQIVSVDTLKPLPPSKLGEIWVRGPNMMQGYFNNPQATELTIDKQGWVHTGDLGYFDEKGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHHEILDAVVIPFPDAEAGEVPIAYVVRSPNSLLTEEDVQKFIADQVAPFKRLRRVTFVKSIPKSPSGKILRRVLIEKVRSKL
>Nsi10884.mRNA1
MDQTQTKQKEIIFRSKLPDLYIPKHLPLHSYCFENISKFSSRPCLINATGEVHTYAEVELTARKVASGLNKLGVKQGETIMILLQNSPEFIFAFLGASYRGANSTMANPFFTPAEVVKQAKASNAKIIITQASHVEKVKDYATQNDVKVMCIDSPPEGCLHFSELTQADENEMPEVEISPDDVVALPYSSGTTGLPKGVMLTHRGQVTSVAQQVDGENPNLYMHSEDVMLCVLPLFHVYSLNSVLFCGLRVGAALLIMQKFEIVTVLELIQKYKVTIAPVVPPIVLAIAKSPAVDNYDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPDTGASLPRNQPGEICIRGDQVMKGYLNDPEATERTIDKQGWLHTGDIGYIDDDEELFIVDRLKELIKYKGFQVAPAELEAMLLNHPKISDAAVVPMKDDAAGEVPVAFVVRSKGSDVTEDEIKQYISKQVVFYKRLNRVFFIDAIPKAHSGKILRKDLRAKLAAGFPN
>Nsi31379.mRNA1
METKREPEEFIFRSKLPDIYIPNHLPLHTYCFENISQFSSRPCVINGATGDIYSYADVELTARKVAAGLDKLGIQQGDVIMLLLQNSPEFVFAFLGASYRGATSTTANPFYTPAEIAKQAIASKAKLIITQSCYAEKADEKDMPAVKINPDDAVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMQKFEINALMELVHKYKVTIAPFVPPIVLAIAKSPVVDKYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGSCGTVVRNAEMKIVDPDTGASLTRNQAGEICYLNDPEATERTIDKEGWLHTGFQVAPCGARSIVDSPIPTSPMLLLYP
>Nsi24220.mRNA1
MINLNPNPNPNPFHLSVMAQDEEHIFRSLYPSVPVPNDVTLPDFVLQDAELYASKVAFVEAVTGKEYTYGEVVRDIRRFAKALRSLGLRKGKVVVVVLPNVAEYAIVALGIMMAGGVFSGANPSAHASEIKKQVEVADAKLIVTNRSTYEKVKALELPVIILGEEHVEGTMNWDGLLEAADKASTDITNIETIHQTDLCALPFSSGTTGVSKGVMLTHHNLVANLCSSLFSVGPELVGQVTILGLMPFFHIYGMTGICCATLRNKGKVVTMQRYEIEPFLNALIAHEVTFAPIVPPIILELVKNPIVDEFYVRKLKLRSIMTAAAPLAPEILNAFEHKFPGVQVQEAYGMTEHSCITLTHGNPSKGHRNAKRNSVGFILPNMELKFIDSETGRSLPNNSPGEICVRSQCVMKGYYKNEEETAHTIDKNGWLHTGDVGFIDDDGDVFIVDRIKELIKYKGFQVAPAELEGILLTHPSIEDAAVLGLPDEEAGEIPAACVVMNPNAKESEMEIMSYVASNVANYKRVRMLQFVDKIPTSPSGKIMRRLLKEEMLKKMKNKPKAIPTLQSN
>Nsi37344.mRNA1
MEAKQDQQEFIFRSKLPDIYIPNHLPLHTYCFENISQFSSRPCIINGPTGDIYTYADVELTSRKVAAGLDKLGIQQGDVIMLLLQNSPEFVFAFLGASYRGATSTTANPFYTPAEIAKQVKASKAKLIITQSCYTEKVMEFGKENDVKIMCIDSPLEGCLHFSELTQADEKDIPAVKIHPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHRDDVIMCVLPLFHIYSLNSVLLCALRVGAAILIMQKFEINTLLELVQKYKVTIAPFVPPIVLAIAKSPVVDKYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKPGSCGTVVRNAEMKIVDPDTAASLPRNQAGEICIRGNQIMKGYLNDPEATENTIDKERWLHTGDIGFIDDNDELFIVDRLKELIKYKGFQVAPAELEAMLISHPNISDAAVVPMKDDTAGEVPVAFVVKAIGSKITEDEIKQYISKQVVFYKRINRVFFTETIPKAPSGKILRKDLRAKLATGLPN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University