Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Nymphaea colorata
Species name
Pocket water lily (Nymphaea colorata) is a species of flowering plant in the family Nymphaeaceae (water-lily family).
Species info
Gene sequence info
Nymphaea_colorata.HI4_OMT.fa
Download
>rna-XM_031647342.1
MEQKLDAQGRNMDVMAQGNAGRVTGSYMKSFSLKAAIELGIADIIHRHGRPLTASQLAAKIPISSTVDVSCLDRLMRFLVYTGVFAQVRKPAGEGAEEVSYGLTPVSSLLVRDNPHCFIPLAMLSLHPLAVAAWDNLGAWFRSEEGCPTAFEAKHGEPFWIKASTDAAINKVFNDAMSSTSNLSAGRMIAGCSEVFEGVRSLVDVGGGIGTLTSVIANAFPHIKCMVLDLPHVVASSPLQLPNVVAIGGDMFESVPPADAVLLKEILHDWDDKMCIKILARCKEAIPKRGGKVIVVDVVRKKCADQVFDEVQLMLDLHMMVMHNGKERSEEEWNKLFTDAGFSGYKIVTSLGAHAVIEVFP
>rna-XM_031618417.1
MEEKMNVQDQGTLKQASQAHLQNLLNSYMKSYSVKAAIELGIADVIHSHGQPLTASQLAAKIPISSSVDVLRLDRLMRLLVHTGVFAEEKRMAGEKGEEVLYGLTPVSRLMLRDSPVSMSPLAMLSLHPLYVSAWDNLAAWFRSTEGYPTAFEAKHGEPLWVKASNYPALNKLFNESMSNLSKLLMGSMIERCGEVFKGVSSLVDVGGGDGTVAGLIADAFPHIKCMVLELPHVVGSSSLQLPNVVAIGGDMFESVPPADAVLLKAIMHNWDDDKCVKILTRCKEAIPNPGGKVLIVDIVSKKWADQVFNEMQLNHDLIMTVFFDGKERNEEDWKKLIIEAGFSSYKIVTTVGVYSLIEVFP
>rna-XM_031617203.1
MERNMHVQKKTTELTVRAHLESLMISYMKSYSVKAAIELGIADIIHSAGGPLTASQLAAKIPISSTVEVPCLDRLMHLLVHTGVFAEELKLSGEEEHEVSYGLTPMSRLLVRDDPYCMSPLAMLSLHPMAVSAWDNLAAWFRSGEGHPTVFEAKHGEPFWKEVSLDPAMNKVFNDAMSSTSKQFISRMVEGCSEVFEGMSSLVDVGGGNGTVAGLIADAFPHIKCMVLDQPHVVAASSPPPNVVAIGGDMFESIPPADAVFLKRILHDWDEEECLKILRRCKEAIPKPGGKVIVADIVRKKWDDQVFNEVQLMLDLHMMVLHNGKERNEEEWKKLFMDAGFSSYKIVTTMGVHSLIELCP
>rna-XM_031627082.1
MEEKMNVQEQGTMEQASQAHLQNLFNSYMKSYSVKAALELGIADVIHSHGQPLTAPQLAAKIPISSSVDVSRLDRLMRLLVHTGVFAEEKSLAGEEGEEVLYGLTPVSKLMLRDSPVSMSPLVMLGVHPLFVSAWDNLAAWFRSTEGYPTAFEAKHGEPLWIKASHDPALNKLFNESMSSLSKLLMGSMVERCGEVFKGVSSLVDVGGGDGTVAGLIADAFPHIKCMLLDLPHVVASSSLQIPNVVAIGGDMFESVPPADAVLLKAIMHNWDDDNCVRILTRCKEAIPNPGGKVLIVDIVSKKWADQVFNEMQLIHDLIMMVFFEGKERNEEDWKKLIIDASFSSYKIVTTVGVYSLIEIFP
>rna-XM_031648817.1
MEEKNVQERPVDQQMAEAHLETLSCSYQKSYSLKAAIKLGIADIIHGHGQPLTASQLATEIPINSSADASCLDRLMRLLVHAGVFAEEKKKTGTGEVLYALTPVSRLLLREGQHNLSALTMLSLHPLIVSTWDNLAAWLQSGEGNWHRTAFEAKHGEPLWVKASLDPKINKLFNEAMSSMCKLFMGCMVERCGEVFKEVSSLVDVGGGNGTVASIIADAFPHIKCMVLDLPHVVAASPPQPNVVAIGGDMFESVPPADAVLIKGVMHDWDDESCVKILMRCKEAIPKLGGKVIVVEIVKKKWPQQVFNELQLVLDLHMMVMNNGKERNEEEWKKLFIDAGFSSYKIVTSVGIHSLIEVFP
>rna-XM_031647928.1
MDQKMNLQEGTTMDDQITAEAHLETISRSYVRSYSLKAAIDLGIPDIIHSHGQPLTASQLAAKIPINPPADASCLDRLMRLLVHAGVFAEETKQTDAEEGEEVSHYGLTPVSRLLLRDGRYNLSSLAVLNLHPLVVSAWDNLAAWLRSGESHPTAFQAKHGEPLWVKASLDPTINKLFGEAMSSVAKLFMGCMLERCGEVFEGVSSLVDVGGGNGTVAALIAEAFPHIKCMVLDLPHVVAASPPRQNVVAIGGDMLESVPPADAVLLKAILHDWDDKDCVKILRQCREAIPEPGGKVIVVDVVRKKWPHQVFNELQLLVDLQMMVMNNGRERTEEEWKKLFIDAGFSSYKIVTSVGVHSLIEVRP
MEQKLDAQGRNMDVMAQGNAGRVTGSYMKSFSLKAAIELGIADIIHRHGRPLTASQLAAKIPISSTVDVSCLDRLMRFLVYTGVFAQVRKPAGEGAEEVSYGLTPVSSLLVRDNPHCFIPLAMLSLHPLAVAAWDNLGAWFRSEEGCPTAFEAKHGEPFWIKASTDAAINKVFNDAMSSTSNLSAGRMIAGCSEVFEGVRSLVDVGGGIGTLTSVIANAFPHIKCMVLDLPHVVASSPLQLPNVVAIGGDMFESVPPADAVLLKEILHDWDDKMCIKILARCKEAIPKRGGKVIVVDVVRKKCADQVFDEVQLMLDLHMMVMHNGKERSEEEWNKLFTDAGFSGYKIVTSLGAHAVIEVFP
>rna-XM_031618417.1
MEEKMNVQDQGTLKQASQAHLQNLLNSYMKSYSVKAAIELGIADVIHSHGQPLTASQLAAKIPISSSVDVLRLDRLMRLLVHTGVFAEEKRMAGEKGEEVLYGLTPVSRLMLRDSPVSMSPLAMLSLHPLYVSAWDNLAAWFRSTEGYPTAFEAKHGEPLWVKASNYPALNKLFNESMSNLSKLLMGSMIERCGEVFKGVSSLVDVGGGDGTVAGLIADAFPHIKCMVLELPHVVGSSSLQLPNVVAIGGDMFESVPPADAVLLKAIMHNWDDDKCVKILTRCKEAIPNPGGKVLIVDIVSKKWADQVFNEMQLNHDLIMTVFFDGKERNEEDWKKLIIEAGFSSYKIVTTVGVYSLIEVFP
>rna-XM_031617203.1
MERNMHVQKKTTELTVRAHLESLMISYMKSYSVKAAIELGIADIIHSAGGPLTASQLAAKIPISSTVEVPCLDRLMHLLVHTGVFAEELKLSGEEEHEVSYGLTPMSRLLVRDDPYCMSPLAMLSLHPMAVSAWDNLAAWFRSGEGHPTVFEAKHGEPFWKEVSLDPAMNKVFNDAMSSTSKQFISRMVEGCSEVFEGMSSLVDVGGGNGTVAGLIADAFPHIKCMVLDQPHVVAASSPPPNVVAIGGDMFESIPPADAVFLKRILHDWDEEECLKILRRCKEAIPKPGGKVIVADIVRKKWDDQVFNEVQLMLDLHMMVLHNGKERNEEEWKKLFMDAGFSSYKIVTTMGVHSLIELCP
>rna-XM_031627082.1
MEEKMNVQEQGTMEQASQAHLQNLFNSYMKSYSVKAALELGIADVIHSHGQPLTAPQLAAKIPISSSVDVSRLDRLMRLLVHTGVFAEEKSLAGEEGEEVLYGLTPVSKLMLRDSPVSMSPLVMLGVHPLFVSAWDNLAAWFRSTEGYPTAFEAKHGEPLWIKASHDPALNKLFNESMSSLSKLLMGSMVERCGEVFKGVSSLVDVGGGDGTVAGLIADAFPHIKCMLLDLPHVVASSSLQIPNVVAIGGDMFESVPPADAVLLKAIMHNWDDDNCVRILTRCKEAIPNPGGKVLIVDIVSKKWADQVFNEMQLIHDLIMMVFFEGKERNEEDWKKLIIDASFSSYKIVTTVGVYSLIEIFP
>rna-XM_031648817.1
MEEKNVQERPVDQQMAEAHLETLSCSYQKSYSLKAAIKLGIADIIHGHGQPLTASQLATEIPINSSADASCLDRLMRLLVHAGVFAEEKKKTGTGEVLYALTPVSRLLLREGQHNLSALTMLSLHPLIVSTWDNLAAWLQSGEGNWHRTAFEAKHGEPLWVKASLDPKINKLFNEAMSSMCKLFMGCMVERCGEVFKEVSSLVDVGGGNGTVASIIADAFPHIKCMVLDLPHVVAASPPQPNVVAIGGDMFESVPPADAVLIKGVMHDWDDESCVKILMRCKEAIPKLGGKVIVVEIVKKKWPQQVFNELQLVLDLHMMVMNNGKERNEEEWKKLFIDAGFSSYKIVTSVGIHSLIEVFP
>rna-XM_031647928.1
MDQKMNLQEGTTMDDQITAEAHLETISRSYVRSYSLKAAIDLGIPDIIHSHGQPLTASQLAAKIPINPPADASCLDRLMRLLVHAGVFAEETKQTDAEEGEEVSHYGLTPVSRLLLRDGRYNLSSLAVLNLHPLVVSAWDNLAAWLRSGESHPTAFQAKHGEPLWVKASLDPTINKLFGEAMSSVAKLFMGCMLERCGEVFEGVSSLVDVGGGNGTVAALIAEAFPHIKCMVLDLPHVVAASPPRQNVVAIGGDMLESVPPADAVLLKAILHDWDDKDCVKILRQCREAIPEPGGKVIVVDVVRKKWPHQVFNELQLLVDLQMMVMNNGRERTEEEWKKLFIDAGFSSYKIVTSVGVHSLIEVRP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University