Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Nissolia schottii
Species name
Nissolia schottii is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Nissolia_schottii.HI4_OMT.fa
Download
>Nissc11096S05036
MDFKSSKGSEASEVVHGQIHLYKHMFMWMSSMALNFILELGIVDKIHSHGGKPMTLPELASALELHPSRLRHLHAFMRLLTHNGFFSKSGGDAYALTPPSKLLVRSSPTCLSPMLLATFRMCPLYMWHSSKKWLTEGDKELTMFESATGETFWDLLNKEPHMMSIFHDAMATESSMFKLALNECAHVFQSLGGGSLLVDVGGGTGEMTKIIHDAFPHLKCTVFDQPQVVANCQGDENLNFVGGDMFESIPPADAVLLKWVLHNWNDEQARKILKNCKEAISGGQKEGKVIIMDIVIDEVNDDPELTEIKVYYDLTMLTLFHGKEREKKEWEKLIYEVGFTRYTINPIPGFMSLIEVYP
>Nissc12594S05353
MDFKSSKGSEASEVVRGQIHLYKHMFMWMSSMALNFILELGIVDKIHSHGGKPMTLPELASALELHPSRLRHLHAFMRLLTHNGFFSKSGGDAYALTPPSKLLVRSSPTCLSPMLLATFRMCPLYMWHSSKKWLTEGDKELTMFESATGETFWDLLNKEPHMMSIFHDAMATESSMFKLALNECAHVFQSLGGGSLLVDVGGGTGEMTKIIHDAFPHLKCTVFDQPQVVANCQGDENLNFVGGDMFESIPPADAVLLKWVLHNWNDEQARKILKNCKEVISGGQKEGKVIIMDIVIDEVNDDPELTEIKVYYDLTMLTLFHGKEREKKEWEKLIYEVGFTRYTINPIPGFMSLIEVYP
>Nissc26735S19795
MDFNGSSEEIELYHAQIHLYKHVYHWVSSMALKSAMELGIADIIHAHGKPITLPELAAALKLHPSKVDILRSFLRLLTHNGFFAKTKLGEQQEEAYALTPPSKLLVRGKPTCHMSTVCGALDATSLDMWHSSNKWFNEQKEQTLFQSATGKSFWDFLNKTPRILSLFQDAMAADSYMFKLALNECAHVFNNLGSLVDVGGGTGAVTKLIHEAFPHLQCTVFDQPEVVANCQGSQNLNFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNCKKAISGKGKDGKVIIIDIAIDEANDNFELTELKLDYDLVMRAMFNGKERDMKEWEKLIYDAGFSRYTIIPICGFKSLIEVYP
>Nissc524725S10175
MDFNGSNGSSEEIELYHAQIHLYKHVYHWVSSMALKSAMELGIADIIHAHGKPITLPELAAALKLHPSKVDILRSFLRLLTHNGFFAKTKLGEQQEEAYALTPPSKLLVRGKPTCHMSTVCGALDATSLDMWHSSNKWFNEQKEQTLFQSATGKSFWDFLNKTPRILSLFQDAMAADSYMFKLALNECAHVFNNLGSLVDVGGGTGAVTKLIHEAFPHLECTVFDQPEVVANCQGSENLNFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNCKKAISGKGKDGKVIIIDIAIDEANDNFELTELKLDYDLVMRAMFNGKERDMKEWEKLIYDAGFSRYTIIPICGFKSLIEWVLHDWNDELSLKILKNCKEAISGRGKDGKVIIIDIAIDEETDDRELTELKLDYDLVMLTMFNGKEREKKEWEKLIYEAGFSRYTITPICGFKSLIEVYP
>Nissc524725S27664
MDPKISNGGSEESELYHAQIHLYKHVYNWVSSMALKSAMELGVADIIHAHGKPITLRELAAALKLHPSKVGNLHRFLRLLTHNGFFTKTKSGEQEEEAYGLTPPSKLLIRSKPTCLGSVVRGALDASSLDMWRSSKKWFTEQKEQTLFESDTGESFWDFLNKGGPDTLSMFQDAMAADSYMFKLALNECAPVFNNLGSLVDVGGGTGGVTKLIHEAFPHLKCTVFDQPEVVANCQGSENLNFVGGDMFKSIPAADAVLLKWVLHDWNDELSLKILKNCKEAISGRGKDGKVIIIDIAIDEETDDRELTELKLDYDLVMLTMFNGKEREKKEWEKLIYEAGFSRYTITPICGFKSLIEVYP
>Nissc1780S16621
MDVNLEQGAEELFQAQSHLYKYTYHFIGSMCLKSAVHLGIPDIIHNHGKPIAINELVLALQIDPNKSSHVYRLIRLLVHAGFFVTTKVATEEVGYDLTPSSRLLLKDTVPTLSPFVRAVFHPALVHPPGFLGEWFHSNEIAPNQEFNGLFNEAMISNSGMMNLVIKDCKSVFEGLDSLVDVGSGKGAMARLVSESLPNLQCTAPDLPHVIENLQDGKNLKFVGGDMFQYIPSADAILLKLVLHAYSDEDCVKRTHFKIRQGRKIINEKNDKHELTEVKLSFYLLMMVVVTGREREEKEWEKLFLEAGFSHYKITHIFGMRSLIEKLDQKQRIEQEKKMDTNQVKGAEELFVAQSHIYNYTYHFIGAMCLKSAVHLGIPDIIHNHGKPITLNELVLALQIDPNKSSHLYRLMRFLVHAGFFVTTKAAKEEEEEEVGYDLTPSSRLLLKETVPTLSPFVRAMFYPALVHPSEFLGEWFHSNEITPFHTAFGTGFWDYLSKDQEYVFEGLDSLVDVGGGKGAVARLVSEAMPGLKCTVLDLPHVVENLQDGNNLKFVGGDMLQSIPSADAILLKLVLHAYSDEDCVKVLKKCREAISSKGNGGKVIIIDIVINEKNDDKHELTKAKLLFDVLMMVMVTGKEREEKEWEKLFLEAGFSHYKITPIFGMRSLIEVYP
MDFKSSKGSEASEVVHGQIHLYKHMFMWMSSMALNFILELGIVDKIHSHGGKPMTLPELASALELHPSRLRHLHAFMRLLTHNGFFSKSGGDAYALTPPSKLLVRSSPTCLSPMLLATFRMCPLYMWHSSKKWLTEGDKELTMFESATGETFWDLLNKEPHMMSIFHDAMATESSMFKLALNECAHVFQSLGGGSLLVDVGGGTGEMTKIIHDAFPHLKCTVFDQPQVVANCQGDENLNFVGGDMFESIPPADAVLLKWVLHNWNDEQARKILKNCKEAISGGQKEGKVIIMDIVIDEVNDDPELTEIKVYYDLTMLTLFHGKEREKKEWEKLIYEVGFTRYTINPIPGFMSLIEVYP
>Nissc12594S05353
MDFKSSKGSEASEVVRGQIHLYKHMFMWMSSMALNFILELGIVDKIHSHGGKPMTLPELASALELHPSRLRHLHAFMRLLTHNGFFSKSGGDAYALTPPSKLLVRSSPTCLSPMLLATFRMCPLYMWHSSKKWLTEGDKELTMFESATGETFWDLLNKEPHMMSIFHDAMATESSMFKLALNECAHVFQSLGGGSLLVDVGGGTGEMTKIIHDAFPHLKCTVFDQPQVVANCQGDENLNFVGGDMFESIPPADAVLLKWVLHNWNDEQARKILKNCKEVISGGQKEGKVIIMDIVIDEVNDDPELTEIKVYYDLTMLTLFHGKEREKKEWEKLIYEVGFTRYTINPIPGFMSLIEVYP
>Nissc26735S19795
MDFNGSSEEIELYHAQIHLYKHVYHWVSSMALKSAMELGIADIIHAHGKPITLPELAAALKLHPSKVDILRSFLRLLTHNGFFAKTKLGEQQEEAYALTPPSKLLVRGKPTCHMSTVCGALDATSLDMWHSSNKWFNEQKEQTLFQSATGKSFWDFLNKTPRILSLFQDAMAADSYMFKLALNECAHVFNNLGSLVDVGGGTGAVTKLIHEAFPHLQCTVFDQPEVVANCQGSQNLNFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNCKKAISGKGKDGKVIIIDIAIDEANDNFELTELKLDYDLVMRAMFNGKERDMKEWEKLIYDAGFSRYTIIPICGFKSLIEVYP
>Nissc524725S10175
MDFNGSNGSSEEIELYHAQIHLYKHVYHWVSSMALKSAMELGIADIIHAHGKPITLPELAAALKLHPSKVDILRSFLRLLTHNGFFAKTKLGEQQEEAYALTPPSKLLVRGKPTCHMSTVCGALDATSLDMWHSSNKWFNEQKEQTLFQSATGKSFWDFLNKTPRILSLFQDAMAADSYMFKLALNECAHVFNNLGSLVDVGGGTGAVTKLIHEAFPHLECTVFDQPEVVANCQGSENLNFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNCKKAISGKGKDGKVIIIDIAIDEANDNFELTELKLDYDLVMRAMFNGKERDMKEWEKLIYDAGFSRYTIIPICGFKSLIEWVLHDWNDELSLKILKNCKEAISGRGKDGKVIIIDIAIDEETDDRELTELKLDYDLVMLTMFNGKEREKKEWEKLIYEAGFSRYTITPICGFKSLIEVYP
>Nissc524725S27664
MDPKISNGGSEESELYHAQIHLYKHVYNWVSSMALKSAMELGVADIIHAHGKPITLRELAAALKLHPSKVGNLHRFLRLLTHNGFFTKTKSGEQEEEAYGLTPPSKLLIRSKPTCLGSVVRGALDASSLDMWRSSKKWFTEQKEQTLFESDTGESFWDFLNKGGPDTLSMFQDAMAADSYMFKLALNECAPVFNNLGSLVDVGGGTGGVTKLIHEAFPHLKCTVFDQPEVVANCQGSENLNFVGGDMFKSIPAADAVLLKWVLHDWNDELSLKILKNCKEAISGRGKDGKVIIIDIAIDEETDDRELTELKLDYDLVMLTMFNGKEREKKEWEKLIYEAGFSRYTITPICGFKSLIEVYP
>Nissc1780S16621
MDVNLEQGAEELFQAQSHLYKYTYHFIGSMCLKSAVHLGIPDIIHNHGKPIAINELVLALQIDPNKSSHVYRLIRLLVHAGFFVTTKVATEEVGYDLTPSSRLLLKDTVPTLSPFVRAVFHPALVHPPGFLGEWFHSNEIAPNQEFNGLFNEAMISNSGMMNLVIKDCKSVFEGLDSLVDVGSGKGAMARLVSESLPNLQCTAPDLPHVIENLQDGKNLKFVGGDMFQYIPSADAILLKLVLHAYSDEDCVKRTHFKIRQGRKIINEKNDKHELTEVKLSFYLLMMVVVTGREREEKEWEKLFLEAGFSHYKITHIFGMRSLIEKLDQKQRIEQEKKMDTNQVKGAEELFVAQSHIYNYTYHFIGAMCLKSAVHLGIPDIIHNHGKPITLNELVLALQIDPNKSSHLYRLMRFLVHAGFFVTTKAAKEEEEEEVGYDLTPSSRLLLKETVPTLSPFVRAMFYPALVHPSEFLGEWFHSNEITPFHTAFGTGFWDYLSKDQEYVFEGLDSLVDVGGGKGAVARLVSEAMPGLKCTVLDLPHVVENLQDGNNLKFVGGDMLQSIPSADAILLKLVLHAYSDEDCVKVLKKCREAISSKGNGGKVIIIDIVINEKNDDKHELTKAKLLFDVLMMVMVTGKEREEKEWEKLFLEAGFSHYKITPIFGMRSLIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University