Enzyme
Isolated from the plants Perilla frutescens var. crispa, Verbena hybrida [1], Dahlia variabilis [2] and Gentiana triflora (clustered gentian) [3]. It will also act on anthocyanidin 3-O-(6-O-malonylglucoside) [2] and is much less active with hydroxycinnamoylglucose derivatives [3]. There is no activity in the absence of the 3-O-glucoside group.
PF3R4
Enzyme
Anthocyanidin 3-O-glucoside 5-O-glucosyltransferase 1
Enzyme name
EC:2.4.1.298
Entry
Transferases;Glycosyltransferases;Hexosyltransferases
Class
UDP-alpha-D-glucose:anthocyanidin-3-O-beta-D-glucoside 5-O-glucosyltransferase
Sysname
UDP-alpha-D-glucose + an anthocyanidin 3-O-beta-D-glucoside = UDP + an anthocyanidin 3,5-di-O-beta-D-glucoside [RN:R10262]
Reaction
UDP [CPD:C00015];anthocyanidin 3,5-di-O-beta-D-glucoside [CPD:C06361]
Product
Species information
Nicotiana attenuata
Species name
Nicotiana attenuata is a species of eudicot in the family Solanaceae (nightshade family).
Species info
Gene sequence info
Nicotiana_attenuata.PF3R4.fa
Download
>rna-XM_019393987.1
MVQPHVLLVTFPAQGHINPCIQFAMRLIRMGIEVTFATSVFAHRRMAKTATSTLPKGLNFAAFSDGYDDGFKVDEHDSRHYMSEIKSRGSETLKDIILKSSDEGRPVTSLVYSLLLPWAANVAREFHIPCALLWIQPATVLDIYYYYFNGYEDAIKGSTNDPNWCIQLPKLPLLKSQDLPSFLLSSSNNEGKYSFALPTFKEQLDTLDVEENPKVLVNTFDALEKEELNAIEKYSLIGIGPLIPSSFLDGKDPLDSSFGGDLFQKSNDYIEWLNSKANSSVVYISFGSLLNLSKNQKEEIAKGLIEIQRPFLWVIRDQENGKGDEKEEEKLSCMMELEKQGKIVPWCSQLEVLTHPSLGCFVSHCGWNSTLESLSSGVPVVAFPHWTDQGTNAKLIEDVWKIGVRLKKNEDGVVESEEIKRCIEMVMDGGEKGEEMRRNAQKWKELAREAVKEGGSSDMNLKAFVQEVGKGC
>rna-XM_019406273.1
MAKMKDNCHVLLVTFPSQGQINPSLQFAKCLIKLGVNVTFSTSLSAQNCMLNNLPSIEGLTFAPFSDGYDNGYPNVGKSLDEYREFYASFIALGSEFVSRIFTDRAKEGRPFSRIIYTTVLSWVGIVAKRLNAPATLLWIQPATLLDIYYYYFTSYKDAFKNCPDDEDKSLEIPGLPFLVGTREIPSFLLSSDGLMDWLIQTMKEHVELINSESNEIVLVNTFDALEFEALRAIKNVNMVGIGPLVPSSFLDGKNPSDTCYGCNLRQCSKDYMEWLNSKPSASVVYVAFGSYSELPKQMMEEIAKGLVQSERPFLWVVNGEKPLENLSCKDELEKQGKIVSWCSQVEVLQHPSLGCFLMHCGWNSTLESLVFGIPVVACPLWTDQGCNSTLIQDVWKTGVRVNANEEGVVEGAEFKRCIEIVMEDRKEGEELRKNAKKWRDIAKNTMKEDGSSNVNLKAYVDEVLKPCIRDYERCINS
>rna-XM_019406292.1
MKKHHFLIISLPAQGHINPTLQLAKNLARAGARCTFVTTVHGFRKMNNLPSIDGLFYASISDGHDDGKPKEMYFGDYLNDLKRVGSENLKNLLQKFTDDGYPVTCLVYTILFTWVAEVAREYHAPSAFLAIQCAAAFAIYYHLFTTNNGIYSSTTEIEPSFPIKLPESPLFSRDDIPTFLLQSDSASSFMIPVMREHIKILENDPNPRVLINTFDALEEKSLKIFEKIGVSSIGPLIPSAFCDGNDVNDKSFGCELFDKSENYSQWLDSKAEGSVVYISFGSLAVLKEEQKEEILKGLLESERPFLWVIRTSNKDDKIKNENYGLNGKGMIVPWCSQMEVLFHKSIGCFMSHCGWNSTLESIAAGVPLIGYPQFSDQVTNIKMVEEVWGTGVRARPEEGIVKREELKRCLEILMGGGEKGNEIRRNVKKYGDLAMEAVKEGGSSHYNLNKFIDSL
>rna-XM_019407746.1
MENLKNECHVLIAIFPGQGHINPSLQFSKKLINLGVKVTLSCSLTAFNRIKNLPKIEGLTFAPFSDGYDGNFKGSFDEYHLLNSAIKIHGSEFIANLIKSKAKNGCPFTRVIYTIIMDWAGLVAKKFNIPSTLFWIQPATVFDIYYYRFTNFANYFKNCDSQDQIIELPGLPSLSSSDFPSFVFDDVKSNDWAVESIKRQIEILNSEENPRILVNTFDALELNALRVLKNVTMVGIGPSIPSSFLDEKDRKDNFFVADMIESENNYMEWLDARANKSVIYIAFGSYAEISNQWMEEIGQGLLKCGRPFLWVIRETLNGEKPEENLTCKDELEKIGRIVRWCSQMEVLKHPSVGCFLTHCGWNSTLESLASGVPIVACPIWNDQVCNAKLIQDVWKIGVRVNANREGIVKRDEFERCIEIVMGGSEEGEELRKNVKKWKDLAKEAMKENGSSNVNLKAYVNEFLLGHC
>rna-XM_019369062.1
MKKHHYLVISLPAQGHVNPTLQMAKNLARAGARATFVTTLYGLKRMNNLPTQERLFYSSFSDGYDDDWMSNTDHTDYMNNLKHEGSKNLKNLVRKFSDEGHPVTFFVYTILLPWVAVVARDLHVPSAFLVIQCGTALAIYNHLFNSTNGVYGSVGDITVTPSFPIEFPELPLFSCKDIPTIVLPNAPHSSVMIPIMREHIQNLENDPNSCVLINTFDALEEKSMRIVDKLRIFSVGPLVPSAFSDGNDPKDKSFGCELFENREKNYRQWLDSKPEDSVIYVSFGSIAVLKKEQKEEILHGLLESERPFLWVMRKGKEEVEEGNNYKNEFDDILLNEKGIIIPWCAQMEVLFHKSIGCFVTHCGWNSTLESMFAGVPIVGCPQFSDQTTNAKMVEEVWGIGVRANAVEGVVGREELKSCLEILMGNGEKGEKIKRNVKKWSDLAVEAVKIGGSSHDNLKKFLEGL
>rna-XM_019369068.1
MEILKDDCHVLIITFPGQGNINPCIQFSKRLVKLGVNVTFSTSLTAFNRITNLPTIEGLTFAPFSDGFDGNFKGSMNNFHEFNNSFRTHGSEFMINLVKTRAKEGNPFKRIIYTTLTGWVGLLATSINVPSTFLWIQPATVLDIYYYFFNGYEDTIKNFPKDKFLELRGLPLLSPRDLPSFVFADSNDWTLLSMKDHVELMDSEINQRVLVNTFDALEPDALSVLKNVNMVGIGPLIPSAFLDGNDSSDTSFGADMRQDSRDYIEWLDSKAKESVIYIAFGSYSMIPNQLMEEIAQGLVLCKRPFLWVIREGQNGEKPFEKLSCKEELEELGKTVSWCSQVEVLQHPSLACFLTHCGWNSTIESLASGVPVVACPLWTDQLCNAKLIQDVWKTGVRVNANDEGVIERNEFERCIEIVVGDGEKGKELRKNAKKWRNLAKDSMKEDGSSNVNLKAYVHEVLLGE
>rna-XM_019376187.1
MEKLKNECHVLVVTFPGQGHINPSLQFSKKLVNLGVKVTFSTSLTAFNRIKNLPTIEGLTFAPFSDGYDGNFKGSVDELHSFYSSLISHGSEFVTRLVASRVAEGRPFTCIIYTTVMAWVGLVAKNINVPSTLFWIQPTTALDIYYYCFTDYNNNIFSIFPQVGSNYADYFKNCSQDQVVELPGLPQLSRRDFPSFVFTDVNSSYGWGVKSIIDHIELLNSEENPRVLVNTFDALEPNALRVLKNLTMVGIGPSIPSAFVGGKDPSDNSFGADLRRSSENYMEWLDTRSKESVIYIAFGSYSEISSQLMEEIAQGLVKCGRPFLWVIREGKEGKKLGGKLSCKEELEQKGKIVSWCSQVEVLQHPSLGCFLTHCGWNSILESLSSGIPVVACPLWTDQGCNAKLIQDVWKTGVRVNANEEGLVERDEFTRCIEIVMEGEKREELRENAKKWEDLAKEAMNENGSSNVNLKAYVNEILLGHY
MVQPHVLLVTFPAQGHINPCIQFAMRLIRMGIEVTFATSVFAHRRMAKTATSTLPKGLNFAAFSDGYDDGFKVDEHDSRHYMSEIKSRGSETLKDIILKSSDEGRPVTSLVYSLLLPWAANVAREFHIPCALLWIQPATVLDIYYYYFNGYEDAIKGSTNDPNWCIQLPKLPLLKSQDLPSFLLSSSNNEGKYSFALPTFKEQLDTLDVEENPKVLVNTFDALEKEELNAIEKYSLIGIGPLIPSSFLDGKDPLDSSFGGDLFQKSNDYIEWLNSKANSSVVYISFGSLLNLSKNQKEEIAKGLIEIQRPFLWVIRDQENGKGDEKEEEKLSCMMELEKQGKIVPWCSQLEVLTHPSLGCFVSHCGWNSTLESLSSGVPVVAFPHWTDQGTNAKLIEDVWKIGVRLKKNEDGVVESEEIKRCIEMVMDGGEKGEEMRRNAQKWKELAREAVKEGGSSDMNLKAFVQEVGKGC
>rna-XM_019406273.1
MAKMKDNCHVLLVTFPSQGQINPSLQFAKCLIKLGVNVTFSTSLSAQNCMLNNLPSIEGLTFAPFSDGYDNGYPNVGKSLDEYREFYASFIALGSEFVSRIFTDRAKEGRPFSRIIYTTVLSWVGIVAKRLNAPATLLWIQPATLLDIYYYYFTSYKDAFKNCPDDEDKSLEIPGLPFLVGTREIPSFLLSSDGLMDWLIQTMKEHVELINSESNEIVLVNTFDALEFEALRAIKNVNMVGIGPLVPSSFLDGKNPSDTCYGCNLRQCSKDYMEWLNSKPSASVVYVAFGSYSELPKQMMEEIAKGLVQSERPFLWVVNGEKPLENLSCKDELEKQGKIVSWCSQVEVLQHPSLGCFLMHCGWNSTLESLVFGIPVVACPLWTDQGCNSTLIQDVWKTGVRVNANEEGVVEGAEFKRCIEIVMEDRKEGEELRKNAKKWRDIAKNTMKEDGSSNVNLKAYVDEVLKPCIRDYERCINS
>rna-XM_019406292.1
MKKHHFLIISLPAQGHINPTLQLAKNLARAGARCTFVTTVHGFRKMNNLPSIDGLFYASISDGHDDGKPKEMYFGDYLNDLKRVGSENLKNLLQKFTDDGYPVTCLVYTILFTWVAEVAREYHAPSAFLAIQCAAAFAIYYHLFTTNNGIYSSTTEIEPSFPIKLPESPLFSRDDIPTFLLQSDSASSFMIPVMREHIKILENDPNPRVLINTFDALEEKSLKIFEKIGVSSIGPLIPSAFCDGNDVNDKSFGCELFDKSENYSQWLDSKAEGSVVYISFGSLAVLKEEQKEEILKGLLESERPFLWVIRTSNKDDKIKNENYGLNGKGMIVPWCSQMEVLFHKSIGCFMSHCGWNSTLESIAAGVPLIGYPQFSDQVTNIKMVEEVWGTGVRARPEEGIVKREELKRCLEILMGGGEKGNEIRRNVKKYGDLAMEAVKEGGSSHYNLNKFIDSL
>rna-XM_019407746.1
MENLKNECHVLIAIFPGQGHINPSLQFSKKLINLGVKVTLSCSLTAFNRIKNLPKIEGLTFAPFSDGYDGNFKGSFDEYHLLNSAIKIHGSEFIANLIKSKAKNGCPFTRVIYTIIMDWAGLVAKKFNIPSTLFWIQPATVFDIYYYRFTNFANYFKNCDSQDQIIELPGLPSLSSSDFPSFVFDDVKSNDWAVESIKRQIEILNSEENPRILVNTFDALELNALRVLKNVTMVGIGPSIPSSFLDEKDRKDNFFVADMIESENNYMEWLDARANKSVIYIAFGSYAEISNQWMEEIGQGLLKCGRPFLWVIRETLNGEKPEENLTCKDELEKIGRIVRWCSQMEVLKHPSVGCFLTHCGWNSTLESLASGVPIVACPIWNDQVCNAKLIQDVWKIGVRVNANREGIVKRDEFERCIEIVMGGSEEGEELRKNVKKWKDLAKEAMKENGSSNVNLKAYVNEFLLGHC
>rna-XM_019369062.1
MKKHHYLVISLPAQGHVNPTLQMAKNLARAGARATFVTTLYGLKRMNNLPTQERLFYSSFSDGYDDDWMSNTDHTDYMNNLKHEGSKNLKNLVRKFSDEGHPVTFFVYTILLPWVAVVARDLHVPSAFLVIQCGTALAIYNHLFNSTNGVYGSVGDITVTPSFPIEFPELPLFSCKDIPTIVLPNAPHSSVMIPIMREHIQNLENDPNSCVLINTFDALEEKSMRIVDKLRIFSVGPLVPSAFSDGNDPKDKSFGCELFENREKNYRQWLDSKPEDSVIYVSFGSIAVLKKEQKEEILHGLLESERPFLWVMRKGKEEVEEGNNYKNEFDDILLNEKGIIIPWCAQMEVLFHKSIGCFVTHCGWNSTLESMFAGVPIVGCPQFSDQTTNAKMVEEVWGIGVRANAVEGVVGREELKSCLEILMGNGEKGEKIKRNVKKWSDLAVEAVKIGGSSHDNLKKFLEGL
>rna-XM_019369068.1
MEILKDDCHVLIITFPGQGNINPCIQFSKRLVKLGVNVTFSTSLTAFNRITNLPTIEGLTFAPFSDGFDGNFKGSMNNFHEFNNSFRTHGSEFMINLVKTRAKEGNPFKRIIYTTLTGWVGLLATSINVPSTFLWIQPATVLDIYYYFFNGYEDTIKNFPKDKFLELRGLPLLSPRDLPSFVFADSNDWTLLSMKDHVELMDSEINQRVLVNTFDALEPDALSVLKNVNMVGIGPLIPSAFLDGNDSSDTSFGADMRQDSRDYIEWLDSKAKESVIYIAFGSYSMIPNQLMEEIAQGLVLCKRPFLWVIREGQNGEKPFEKLSCKEELEELGKTVSWCSQVEVLQHPSLACFLTHCGWNSTIESLASGVPVVACPLWTDQLCNAKLIQDVWKTGVRVNANDEGVIERNEFERCIEIVVGDGEKGKELRKNAKKWRNLAKDSMKEDGSSNVNLKAYVHEVLLGE
>rna-XM_019376187.1
MEKLKNECHVLVVTFPGQGHINPSLQFSKKLVNLGVKVTFSTSLTAFNRIKNLPTIEGLTFAPFSDGYDGNFKGSVDELHSFYSSLISHGSEFVTRLVASRVAEGRPFTCIIYTTVMAWVGLVAKNINVPSTLFWIQPTTALDIYYYCFTDYNNNIFSIFPQVGSNYADYFKNCSQDQVVELPGLPQLSRRDFPSFVFTDVNSSYGWGVKSIIDHIELLNSEENPRVLVNTFDALEPNALRVLKNLTMVGIGPSIPSAFVGGKDPSDNSFGADLRRSSENYMEWLDTRSKESVIYIAFGSYSEISSQLMEEIAQGLVKCGRPFLWVIREGKEGKKLGGKLSCKEELEQKGKIVSWCSQVEVLQHPSLGCFLTHCGWNSILESLSSGIPVVACPLWTDQGCNAKLIQDVWKTGVRVNANEEGLVERDEFTRCIEIVMEGEKREELRENAKKWEDLAKEAMNENGSSNVNLKAYVNEILLGHY
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University