Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Nicotiana attenuata
Species name
Nicotiana attenuata is a species of eudicot in the family Solanaceae (nightshade family).
Species info
Gene sequence info
Nicotiana_attenuata.COMT.fa
Download
>rna-XM_019403088.1
MCALQLGIPDVLYKYGKPMSLSDLSAELSLVINPSKVCFLNQHDQDHHYSLTPASRLLLDNEPFNVRSLLLINHDPVFSKAWFESKMILPLLFILLMGNLYGIIFHVDQEPRVLGDIFNDALASDSRLNTNFLITECKHVFEGLSSLVVALCTVHVVEDCKGSGNLEFVGRDMFDNIPHANAILLKCILHDWRDEDCVKIY
>rna-XM_019405044.1
MESSTKSQIPTQIEEERNCTYAMQLLTSSVLPFVLHSTVQLEVFEILAKANDTKLSASQIVTQIPNCKNPDAATMLDRMLFALASYSLFTCSIVELELKDAFLEGGVLFDRVHGVYAFKYSKSDPKFNDVFNKAMINHTTVVMKKILENYKGFENLKTLVDVGGGLGINLKMIASKYPTIKGTNFDLPHVVRHAPSYQGVEHVGGDMFESVPEGDAIFMKWILHDWSDSHNLKLLKNCYKALPDNGKVIVVEAILPVKPNTDTDVVGVLQCDLIVMAQYHGGKERSEQDFRALATETGFKGVNLICCFCNFWVMEFCK
>rna-XM_019370544.1
MASDSQLMSLVVKDCREIFEELDSLVDVGGGTGVMAKTILGAFPHIKCTVLDLPRVVANMPNTDNLKYVGGDMFQSIPSADAILFKVVMHNWSDEDCVKMLKRCREEVKNEGKKGKVIIIDIVLNRDDEEPDMTELIEVNEMKMILQ
>rna-XM_019378737.1
MDISKDQNKNLMSNEEEDLLSVMQLPIGLALNMVLKVTMELGIFDLLSKVGPNAQLSSSQIVSQIPTKNPQATLMIEKILSFLASQSLLSFTLNKDENGVKQSLLYSLTPLSRNLVTNEDGVSIAPTFLLMNDQAMVTPWFHLKDAILEGEIPFNKAHGMGVFEYHGKDKRYAGVTNRSTQSINRTIINKILESYNGFQEVKILVDVGGALGLTMASIVSKYPHIKGVNFDLPHVIKIAPTYPGVEHVSGDMFQTVPQGDAMFMKHVLHDWDDDDCIKILKNCWKSLPNSGKLVLVEHIKPEYPKTDMISKNALFLDIFLMIVTSGGKERTKEEFETLAKKAGFAGFKVINSAYDIWIMEFYK
>rna-XM_019390095.1
MGSTSQSQSNSLTHTEDEAFLFAMQLCSASVLPMVLKSALELDLLELMAKAGPGAAISPTELAAQLSTQNPEAPVMLDRMLRLLATYSVLNCTLRTLSDGSVERLYSLAPVCKYLTKNADGVSVAPLLLMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTMSMKKILEDYKGFEGLNSIVDVGGGTGATVNMIVSKYPSIKGINFDLPHVIGDAPAYPGVEHVGGDMFVSVPKADAIFMKWICHDWSDEHCLKFLKNCYEALPANGKVIIAECILPEAPDTSLATKNTVHVDIVMLAHNPGGKERTEKEFEALAKGAGFTGFARLVALTTLGSWNSTSN
>rna-XM_019370680.1
MDMALSMDKIVISNEEENSMMKAMHLPTGLFLNMVLKAAIELGLFEIIAKANSELSSYDIASQLPAKNPNAPLILERILRFLASQSFLTSNISKDDDGHIHSSYNLTPLGKSFISDKDGSSFAPFLLLMTDPVAVNSWFHLKVAILEGEIPFNKAHGMHAFEYHGKDSRLNMVFNKAMQNISSIEMKRILESYTGFQGVKEVIDVGGGLGVSLASIISKYPNIKGINFDLPHVIKDAPIYAGIEHVGGDMFKSIPKGEVIILKAILHDWDDDHCLKILKNCWEALPENGKVVVIEQIQPEYPENNLLSKHSFSTDMLMITMLNGAKERTKQEFEALAKEAGFSSPKIACRAYYCWVMELYKC
>rna-XM_019375123.1
MENGNGEASNNNNNARLAIMELANMISVPMSLNAVVRLKVADAIWEGGSNAPLSPTEILAKIRPSEGGGGGDAENLQRVLRMLASYGVFAEHIVDDGSQRRYSLTEVGKTLVTDENGLSYGSYVLQHHQDALMRAWTMVHEAVNDSSTEPFAKANGEPAYNYYGTKPEMNNLMLNAMSGVSVPFMKAILESYNGFQGVKTLVDIGGSGGDCLKMILEKHQSIQQGINFDLPEVVAKAPKIPRINHIGGDMFKYVPKGDAIFMKWVLTTWTDDECKQIMKSCYNALPEKGKLIACEPVLPHYTDDSKRTRALLEGDIFVMTIYRAKGKHRTEDEYRQLGRSAGFADCRSLYIDHFFTILEFHKL
>rna-XM_019377131.1
MESSTKSQIPTQSEEERNCTYAMQLLSSSVLPFVLHSTIQLEVFEILAKANDTKLSASEIVSQMPNCKNPDAATMLDRMLYVLASYSLFTCSIVEDEENGGSKRVYGLSQVGKFFVRDEDGASMGPLLALLQDKVFINSWFELKDAVLEGGVPFDRVHGVHAFEYPKSEPKFNDVFNKAMINHTTVVMKKILEKYKGFDNLKALVDVGGGLGVNLKMITTKYPTIKGTNFDLPHVVQHAPSYPGVEHVGGDMFESVPEGDAIFMKWILHDWSDSHNLKLLKNCYKALPENGKVIVVEAILPVKPDTDTAVVGVSQGDLIMMAQNHGGKERSEQEFRSLATTAGFKGVNLICFVCNLWVMEFCK
>rna-XM_019378692.1
MASDSKMMSLVVKDCKQVFEEMDSLVDVGGGTGVIAKNILGAFPHIKCTVLDLPHVVANMPETENLKYVGGDMFQSIPPADAFLFKLVMHD
>rna-XM_019380692.1
MESSTKSQIPTQSEEERNCTYAMQLLSSSVLPFVLHSTIQLEVFEILAKANDTKLSASQIVSQMPNCKNPDAATMLDRMLYVLASYSLFTCSIVEDEENGGSKRVYGLSQVGKFFVRDEDGASMGPLLALLQDKVFINSWFELKDAVLEGGVPFDRVHGVHAFEYPKSDPKFNDVFNKAMINHTTVVIKKILENYKGFDNLKTLVDVGGGLGVNLKMITAKYPTIKGTNFDLPHVVQHAPSYPGVEHVGGDMFGSVPEGDAIFMKWILHDWSDSHNLKLLKNCYKALPDNGKVIVVEAILPVKPDTDTAVVGVSQCDLIMMAQNPGGKERSEQEFRALATEAGFKGVNLICCVCNFWVMEFCK
MCALQLGIPDVLYKYGKPMSLSDLSAELSLVINPSKVCFLNQHDQDHHYSLTPASRLLLDNEPFNVRSLLLINHDPVFSKAWFESKMILPLLFILLMGNLYGIIFHVDQEPRVLGDIFNDALASDSRLNTNFLITECKHVFEGLSSLVVALCTVHVVEDCKGSGNLEFVGRDMFDNIPHANAILLKCILHDWRDEDCVKIY
>rna-XM_019405044.1
MESSTKSQIPTQIEEERNCTYAMQLLTSSVLPFVLHSTVQLEVFEILAKANDTKLSASQIVTQIPNCKNPDAATMLDRMLFALASYSLFTCSIVELELKDAFLEGGVLFDRVHGVYAFKYSKSDPKFNDVFNKAMINHTTVVMKKILENYKGFENLKTLVDVGGGLGINLKMIASKYPTIKGTNFDLPHVVRHAPSYQGVEHVGGDMFESVPEGDAIFMKWILHDWSDSHNLKLLKNCYKALPDNGKVIVVEAILPVKPNTDTDVVGVLQCDLIVMAQYHGGKERSEQDFRALATETGFKGVNLICCFCNFWVMEFCK
>rna-XM_019370544.1
MASDSQLMSLVVKDCREIFEELDSLVDVGGGTGVMAKTILGAFPHIKCTVLDLPRVVANMPNTDNLKYVGGDMFQSIPSADAILFKVVMHNWSDEDCVKMLKRCREEVKNEGKKGKVIIIDIVLNRDDEEPDMTELIEVNEMKMILQ
>rna-XM_019378737.1
MDISKDQNKNLMSNEEEDLLSVMQLPIGLALNMVLKVTMELGIFDLLSKVGPNAQLSSSQIVSQIPTKNPQATLMIEKILSFLASQSLLSFTLNKDENGVKQSLLYSLTPLSRNLVTNEDGVSIAPTFLLMNDQAMVTPWFHLKDAILEGEIPFNKAHGMGVFEYHGKDKRYAGVTNRSTQSINRTIINKILESYNGFQEVKILVDVGGALGLTMASIVSKYPHIKGVNFDLPHVIKIAPTYPGVEHVSGDMFQTVPQGDAMFMKHVLHDWDDDDCIKILKNCWKSLPNSGKLVLVEHIKPEYPKTDMISKNALFLDIFLMIVTSGGKERTKEEFETLAKKAGFAGFKVINSAYDIWIMEFYK
>rna-XM_019390095.1
MGSTSQSQSNSLTHTEDEAFLFAMQLCSASVLPMVLKSALELDLLELMAKAGPGAAISPTELAAQLSTQNPEAPVMLDRMLRLLATYSVLNCTLRTLSDGSVERLYSLAPVCKYLTKNADGVSVAPLLLMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTMSMKKILEDYKGFEGLNSIVDVGGGTGATVNMIVSKYPSIKGINFDLPHVIGDAPAYPGVEHVGGDMFVSVPKADAIFMKWICHDWSDEHCLKFLKNCYEALPANGKVIIAECILPEAPDTSLATKNTVHVDIVMLAHNPGGKERTEKEFEALAKGAGFTGFARLVALTTLGSWNSTSN
>rna-XM_019370680.1
MDMALSMDKIVISNEEENSMMKAMHLPTGLFLNMVLKAAIELGLFEIIAKANSELSSYDIASQLPAKNPNAPLILERILRFLASQSFLTSNISKDDDGHIHSSYNLTPLGKSFISDKDGSSFAPFLLLMTDPVAVNSWFHLKVAILEGEIPFNKAHGMHAFEYHGKDSRLNMVFNKAMQNISSIEMKRILESYTGFQGVKEVIDVGGGLGVSLASIISKYPNIKGINFDLPHVIKDAPIYAGIEHVGGDMFKSIPKGEVIILKAILHDWDDDHCLKILKNCWEALPENGKVVVIEQIQPEYPENNLLSKHSFSTDMLMITMLNGAKERTKQEFEALAKEAGFSSPKIACRAYYCWVMELYKC
>rna-XM_019375123.1
MENGNGEASNNNNNARLAIMELANMISVPMSLNAVVRLKVADAIWEGGSNAPLSPTEILAKIRPSEGGGGGDAENLQRVLRMLASYGVFAEHIVDDGSQRRYSLTEVGKTLVTDENGLSYGSYVLQHHQDALMRAWTMVHEAVNDSSTEPFAKANGEPAYNYYGTKPEMNNLMLNAMSGVSVPFMKAILESYNGFQGVKTLVDIGGSGGDCLKMILEKHQSIQQGINFDLPEVVAKAPKIPRINHIGGDMFKYVPKGDAIFMKWVLTTWTDDECKQIMKSCYNALPEKGKLIACEPVLPHYTDDSKRTRALLEGDIFVMTIYRAKGKHRTEDEYRQLGRSAGFADCRSLYIDHFFTILEFHKL
>rna-XM_019377131.1
MESSTKSQIPTQSEEERNCTYAMQLLSSSVLPFVLHSTIQLEVFEILAKANDTKLSASEIVSQMPNCKNPDAATMLDRMLYVLASYSLFTCSIVEDEENGGSKRVYGLSQVGKFFVRDEDGASMGPLLALLQDKVFINSWFELKDAVLEGGVPFDRVHGVHAFEYPKSEPKFNDVFNKAMINHTTVVMKKILEKYKGFDNLKALVDVGGGLGVNLKMITTKYPTIKGTNFDLPHVVQHAPSYPGVEHVGGDMFESVPEGDAIFMKWILHDWSDSHNLKLLKNCYKALPENGKVIVVEAILPVKPDTDTAVVGVSQGDLIMMAQNHGGKERSEQEFRSLATTAGFKGVNLICFVCNLWVMEFCK
>rna-XM_019378692.1
MASDSKMMSLVVKDCKQVFEEMDSLVDVGGGTGVIAKNILGAFPHIKCTVLDLPHVVANMPETENLKYVGGDMFQSIPPADAFLFKLVMHD
>rna-XM_019380692.1
MESSTKSQIPTQSEEERNCTYAMQLLSSSVLPFVLHSTIQLEVFEILAKANDTKLSASQIVSQMPNCKNPDAATMLDRMLYVLASYSLFTCSIVEDEENGGSKRVYGLSQVGKFFVRDEDGASMGPLLALLQDKVFINSWFELKDAVLEGGVPFDRVHGVHAFEYPKSDPKFNDVFNKAMINHTTVVIKKILENYKGFDNLKTLVDVGGGLGVNLKMITAKYPTIKGTNFDLPHVVQHAPSYPGVEHVGGDMFGSVPEGDAIFMKWILHDWSDSHNLKLLKNCYKALPDNGKVIVVEAILPVKPDTDTAVVGVSQCDLIMMAQNPGGKERSEQEFRALATEAGFKGVNLICCVCNFWVMEFCK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University