Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Nicotiana attenuata
Species name
Nicotiana attenuata is a species of eudicot in the family Solanaceae (nightshade family).
Species info
Gene sequence info
Nicotiana_attenuata.ROMT.fa
Download
>rna-XM_019403088.1
MCALQLGIPDVLYKYGKPMSLSDLSAELSLVINPSKVCFLNQHDQDHHYSLTPASRLLLDNEPFNVRSLLLINHDPVFSKAWFESKMILPLLFILLMGNLYGIIFHVDQEPRVLGDIFNDALASDSRLNTNFLITECKHVFEGLSSLVVALCTVHVVEDCKGSGNLEFVGRDMFDNIPHANAILLKCILHDWRDEDCVKIY
>rna-XM_019393499.1
MAKKENNRADHEQLLQAEAQTWDCILSFVRPYCITCAVELGIPDILHKNGIMTISDLVASLPNLNPSKISFLPILMRILVHSGLLHYQKEVDGYSVTSAGSLLVENEPFSKRSNFLFSQNPIALKPFFSLSHWLQDDLPTAFDTTYGKSMWDYCAEEPKFGHIFNDAMASDSKLISHLLINSEQCKHVFEGLTSLVDVGGGTGTVAMAIANAFPRLNCIVLDLPHVIGDRKGITTGNLEFVAGSMFDSIPYANAILLKFILHNWSDEDCVKLLRKCRESIPSKEKGGKVIVIDIVMEDYSSNNINEQLVQVQHLMDVVMRITHGAKERTKTEWEKLFLDAGFSEYKITANLGFRSLIEIYP
>rna-XM_019396080.1
MANIVKTSSWKMASNLNGSTELLQAQTQTWNHIYNFVSSSAAKCAVQLGIPDVLYKHGKPMCLSDLSAALPLNPSKISFLPILMRSLVQSGFLNEHEDNYYSLTPASRLFVKDDPLSTRSYFLIMHDPCLQKPWFELSSWFQNDFPTAFHTANNGKSLWDYFADEPRGNDIFNDSMANDSRLIANVLITECKHVFEGLTSLVDVGGGTGTVAIAIAKAFPAINCTVLDLPHVIGDLKGSGNLEFVGGSMFEKIPNANAIILKWILHNWGDEDCVKILKKCKESIPSREKGGKVIIIDIVLENPKQKDDSVRAQHNMDMLMMVLFAAKERTKKEWVKLFTEAGFSGYEVTPTLGTRSLIEIYP
>rna-XM_019395896.1
MSRNENGSTDLLRAQAQIWNHMFSFINSSATKCAVQLGIPDVLYKHGKPMSFSDLSAELPLVHPSKVPFLTILMRFLVHSGFLNQHDQNHYSLTPVSRLLATNNLSGTRSHFLINHIQLLQNASFELTSWFQNDSPTAFHTAHGISFWDYFVEKPKLGESFNVAMASDSMLVANVLITECKYVFEGLSSLVDVGGGTGTVAIAIAKAFPMINCTVFDLPHVIGDLKGSGNLEFVGGSMFDKIPNANAILLKWILHDWSDEDCVKILKKCKEAIPCREKGGKVIIIDMVMEDPMLISDEEFVRAQHNMDLLMMVLCAAKERTKKEWEKLFTESGFTQYKITHSLGSRSLIEIYP
>rna-XM_019403901.1
MFIDIGDKAGEVLAAQAHIWNHTFNYINSMSLKCAIQLGIPDIIHSHGRAMTLSELVNALPINKAKGQDYVYRLMRILTHAGFFIQAKVNNENEECYLLSSSSYLLLKDEPMNMIPFLQAQLDPILMDPFHSLSQWFKNGDSTPFATFNGKPLFECAEKERRLNRLFNEAMSSDARLIISIMIEHCKGVFEGLKSIVDVGGGTGTVAKAIADKFPKINCIVFDLPHVIEGYEGSENLSYVRGDMFKSIPSADAILLKHWILHDWSDEECIQILKKCKEAIPSKENGGKFIIDMVLDNQKGDQKTYETQLFWDMLMMGHTSGKERTQQDWAKLFSNAGFSDYKIIPNLGLRSIIEVYP
>rna-XM_019410491.1
MALSNNIGDETREILSAQAHIANHVFNYINSMSLKCAIQLEIPDIIHNHGKPMTLSNLVNALPINKAKGQDCIYRLMRILIHSGFFMQTKINETEEEEEEGYLLTPNSRLLLKNEPLSLVPFVLAQLDPILMDPFQSLSKWLQNDDSTPFATAHGKPLFEYAGDEPKLNYLFNEAMASDARLMISVVIKNGSGIFDGLKSLVDVGGGIGTVAKAISNAFPEVKCTVFDLPHVVEGLDGSNNLSYVGGDMFKSVPSADAILLKWILHDWSDEDCVKILKKCKEAIQSKEKGGKVIIIDIVVDSQNGDNKSFETQLFSDILMMVHVSGKERNPEEWAKLFSDAGFSDYKINPILGLRSVIEVYP
>rna-XM_019377276.1
MSINIGDKAREVLAAQAHIWNHTFSYINSMSLKCAIQLGIPDIIHSHGRAMTLSELVNALPIFEYAEKESGFNCLFNEAMASDAPLIISIMIEHCKEVFEGLKSIVDVGGGTGTTAKAIADAFPKVNCIVLDLPHAGS
>rna-XM_019381194.1
MSMNENRSIDLLLAQTQTWNHMFNFISSSAAKCAIELGIPDVLYKHDKPMSLSDLTAELSLIDHSKVSFLPILMRFLVHSGFLNQHEKEHYSLTAASDLLVKDNPLSMRSYFLIIHDPVLLKAWSCLSDWFQDDSSTAFHTAHEKSLWDYYVQEPRVGDSFNESMASDSRLIANVLITECKHVFEGLTSLVDVGGGTGTVAITIAKAFPAIKCTVLDLPRVVGNLKGSGNVEFVGGSMFEKIPNANAILLKWILHNWSDEDCVKILKKCKESIPSREEGGKVIIIDIVLENPNVTNEFVRAQHYMDLLMMVCYAAKQRTKMEWERLFIDAGFNEYKITPTLGTRSLIEIYP
>rna-XM_019384391.1
MVVPIVGEHETEILHAQTHIWNHIFSFINSMSLKSAIQLNIPDIIHKHGKPMTLDQLANALSINKAKTTHLRRLMRILIHSGFFVKSRIQENDGESEGIDEGYALAPPSRLLLKDEPLSVTPFLLAMLDPILVKPWHHVSEWFENDHPTPFDTAHGRTFWDYAGHEPRLNHFFNDAMASDAKLVMNVVIKYSKDVFEGLNSIVDVGGGTGTVAKSIANAFPDLQCTVFDLPHVVEGLEGNKNLNYVGGDFFVSIPPADALLLKWILHDWSDEESVKILKKCKEAIPSREKGGKVIIIDMMVDNKIGDDESIETQIFFDMLMMVLVTGRERSEKGWAKLFSDAGFSDYKVTPILGLRSLIEVYP
MCALQLGIPDVLYKYGKPMSLSDLSAELSLVINPSKVCFLNQHDQDHHYSLTPASRLLLDNEPFNVRSLLLINHDPVFSKAWFESKMILPLLFILLMGNLYGIIFHVDQEPRVLGDIFNDALASDSRLNTNFLITECKHVFEGLSSLVVALCTVHVVEDCKGSGNLEFVGRDMFDNIPHANAILLKCILHDWRDEDCVKIY
>rna-XM_019393499.1
MAKKENNRADHEQLLQAEAQTWDCILSFVRPYCITCAVELGIPDILHKNGIMTISDLVASLPNLNPSKISFLPILMRILVHSGLLHYQKEVDGYSVTSAGSLLVENEPFSKRSNFLFSQNPIALKPFFSLSHWLQDDLPTAFDTTYGKSMWDYCAEEPKFGHIFNDAMASDSKLISHLLINSEQCKHVFEGLTSLVDVGGGTGTVAMAIANAFPRLNCIVLDLPHVIGDRKGITTGNLEFVAGSMFDSIPYANAILLKFILHNWSDEDCVKLLRKCRESIPSKEKGGKVIVIDIVMEDYSSNNINEQLVQVQHLMDVVMRITHGAKERTKTEWEKLFLDAGFSEYKITANLGFRSLIEIYP
>rna-XM_019396080.1
MANIVKTSSWKMASNLNGSTELLQAQTQTWNHIYNFVSSSAAKCAVQLGIPDVLYKHGKPMCLSDLSAALPLNPSKISFLPILMRSLVQSGFLNEHEDNYYSLTPASRLFVKDDPLSTRSYFLIMHDPCLQKPWFELSSWFQNDFPTAFHTANNGKSLWDYFADEPRGNDIFNDSMANDSRLIANVLITECKHVFEGLTSLVDVGGGTGTVAIAIAKAFPAINCTVLDLPHVIGDLKGSGNLEFVGGSMFEKIPNANAIILKWILHNWGDEDCVKILKKCKESIPSREKGGKVIIIDIVLENPKQKDDSVRAQHNMDMLMMVLFAAKERTKKEWVKLFTEAGFSGYEVTPTLGTRSLIEIYP
>rna-XM_019395896.1
MSRNENGSTDLLRAQAQIWNHMFSFINSSATKCAVQLGIPDVLYKHGKPMSFSDLSAELPLVHPSKVPFLTILMRFLVHSGFLNQHDQNHYSLTPVSRLLATNNLSGTRSHFLINHIQLLQNASFELTSWFQNDSPTAFHTAHGISFWDYFVEKPKLGESFNVAMASDSMLVANVLITECKYVFEGLSSLVDVGGGTGTVAIAIAKAFPMINCTVFDLPHVIGDLKGSGNLEFVGGSMFDKIPNANAILLKWILHDWSDEDCVKILKKCKEAIPCREKGGKVIIIDMVMEDPMLISDEEFVRAQHNMDLLMMVLCAAKERTKKEWEKLFTESGFTQYKITHSLGSRSLIEIYP
>rna-XM_019403901.1
MFIDIGDKAGEVLAAQAHIWNHTFNYINSMSLKCAIQLGIPDIIHSHGRAMTLSELVNALPINKAKGQDYVYRLMRILTHAGFFIQAKVNNENEECYLLSSSSYLLLKDEPMNMIPFLQAQLDPILMDPFHSLSQWFKNGDSTPFATFNGKPLFECAEKERRLNRLFNEAMSSDARLIISIMIEHCKGVFEGLKSIVDVGGGTGTVAKAIADKFPKINCIVFDLPHVIEGYEGSENLSYVRGDMFKSIPSADAILLKHWILHDWSDEECIQILKKCKEAIPSKENGGKFIIDMVLDNQKGDQKTYETQLFWDMLMMGHTSGKERTQQDWAKLFSNAGFSDYKIIPNLGLRSIIEVYP
>rna-XM_019410491.1
MALSNNIGDETREILSAQAHIANHVFNYINSMSLKCAIQLEIPDIIHNHGKPMTLSNLVNALPINKAKGQDCIYRLMRILIHSGFFMQTKINETEEEEEEGYLLTPNSRLLLKNEPLSLVPFVLAQLDPILMDPFQSLSKWLQNDDSTPFATAHGKPLFEYAGDEPKLNYLFNEAMASDARLMISVVIKNGSGIFDGLKSLVDVGGGIGTVAKAISNAFPEVKCTVFDLPHVVEGLDGSNNLSYVGGDMFKSVPSADAILLKWILHDWSDEDCVKILKKCKEAIQSKEKGGKVIIIDIVVDSQNGDNKSFETQLFSDILMMVHVSGKERNPEEWAKLFSDAGFSDYKINPILGLRSVIEVYP
>rna-XM_019377276.1
MSINIGDKAREVLAAQAHIWNHTFSYINSMSLKCAIQLGIPDIIHSHGRAMTLSELVNALPIFEYAEKESGFNCLFNEAMASDAPLIISIMIEHCKEVFEGLKSIVDVGGGTGTTAKAIADAFPKVNCIVLDLPHAGS
>rna-XM_019381194.1
MSMNENRSIDLLLAQTQTWNHMFNFISSSAAKCAIELGIPDVLYKHDKPMSLSDLTAELSLIDHSKVSFLPILMRFLVHSGFLNQHEKEHYSLTAASDLLVKDNPLSMRSYFLIIHDPVLLKAWSCLSDWFQDDSSTAFHTAHEKSLWDYYVQEPRVGDSFNESMASDSRLIANVLITECKHVFEGLTSLVDVGGGTGTVAITIAKAFPAIKCTVLDLPRVVGNLKGSGNVEFVGGSMFEKIPNANAILLKWILHNWSDEDCVKILKKCKESIPSREEGGKVIIIDIVLENPNVTNEFVRAQHYMDLLMMVCYAAKQRTKMEWERLFIDAGFNEYKITPTLGTRSLIEIYP
>rna-XM_019384391.1
MVVPIVGEHETEILHAQTHIWNHIFSFINSMSLKSAIQLNIPDIIHKHGKPMTLDQLANALSINKAKTTHLRRLMRILIHSGFFVKSRIQENDGESEGIDEGYALAPPSRLLLKDEPLSVTPFLLAMLDPILVKPWHHVSEWFENDHPTPFDTAHGRTFWDYAGHEPRLNHFFNDAMASDAKLVMNVVIKYSKDVFEGLNSIVDVGGGTGTVAKSIANAFPDLQCTVFDLPHVVEGLEGNKNLNYVGGDFFVSIPPADALLLKWILHDWSDEESVKILKKCKEAIPSREKGGKVIIIDMMVDNKIGDDESIETQIFFDMLMMVLVTGRERSEKGWAKLFSDAGFSDYKVTPILGLRSLIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University